Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

The cerebellar cortex is a well described structure that provides unique opportunities for studying neuronal properties and development1,2. Of the cerebellar neuronal types (granule cells, Purkinje cells and inhibitory interneurons), granule neurons are by far the most numerous and are the most abundant type of neurons in the mammalian brain. In rodents, cerebellar granule neurons are generated during the first two post-natal weeks from progenitor cells in the outermost layer of the cerebellar cortex, the external granule layer (EGL). The protocol presented here describes techniques to enrich and culture granule neurons and their progenitor cells from post-natal mouse cerebellum. We will describe procedures to obtain cultures of increasing purity3,4, which can be used to study the differentiation of proliferating progenitor cells into granule neurons5,6. Once the progenitor cells differentiate, the cultures also provide a homogenous population of granule neurons for experimental manipulation and characterization of phenomena such as synaptogenesis, glutamate receptor function7, interaction with other purified cerebellar cells8,9 or cell death7

Evidence for ablated flows in the shell of nova DQ Her

High-resolution longslit Halpha spectra of the shell of the old nova DQ Her have been obtained with the William Herschel Telescope using the ISIS spectrograph. An equatorial expansion velocity of 370+/-14 km/s is derived from the spectra which, in conjunction with a narrowband Halpha image of the remnant, allows a distance estimate of 525+/-28 pc. An equatorial ring which exhibits enhanced [NII] emission has also been detected and the inclination angle of the shell is found to be 86.8+/-0.2 degrees with respect to the line of sight. The spectra also reveal tails extending from the clumps in the shell, which have a radial velocity increasing along their length. This suggests the presence of a stellar wind, collimated in the polar direction, which ablates fragments of material from the clumps and accelerates them into its stream up to a terminal velocity of order 800-900 km/s.Comment: 7 pages, 6 figure

RF coupler for high-power CW FEL photoinjector

A high-current emittance-compensated RF photoinjector is a key enabling technology for a high-power CW FEL. The design presently under way is a 100-mA 2.5-cell {pi}-mode, 700-MHz, normal conducting demonstration CW RF photoinjector. This photoinjector will be capable of accelerating 3 nC per bunch with an emittance at the wiggler less than 10 mm-mrad. The paper presents results for the RF coupling from ridged wave guides to hte photoinjector RF cavity. The LEDA and SNS couplers inspired this 'dog-bone' design. Electromagnetic modeling of the coupler-cavity system has been performed using both 2-D and 3-D frequency-domain calculations, and a novel time-domain approach with MicroWave Studio. These simulations were used to adjust the coupling coefficient and calculate the power-loss distribution on the coupling slot. The cooling of this slot is a rather challenging thermal management project

DIVERSITY OF STAPHYLOCOCCAL SPECIES CULTURED FROM CAPTIVE LIVINGSTONE’S FRUIT BATS (PTEROPUS LIVINGSTONII) AND THEIR ENVIRONMENT

Livingstone's fruit bats (Pteropus livingstonii) are critically endangered and a captive population has been established as part of the International Union for Conservation of Nature Species Action Plan. The largest colony, in Jersey Zoo, was sampled for staphylococcal carriage and at infection sites, as disease associated with staphylococci had previously been found. Staphylococci were cultured from swabs from 44 bats (skin, oropharynx, mouth ejecta, skin lesions) and from their enclosure. The isolates were identified by matrix-assisted laser desorption-ionization time of flight mass spectrometry; antimicrobial susceptibility testing was performed by disc diffusion and screening for mecA and mecC. Seventeen species of coagulase-negative staphylococci including Staphylococcus xylosus, S. kloosii, S. nepalensis, and S. simiae were isolated. Staphylococcus aureus was identified from both carriage and lesional sites. These findings suggest S. nepalensis may be part of the normal carriage flora of bats. Antimicrobial resistance rates were low and methicillin-resistant Staphylococcus aureus (MRSA) was not identified. Sampling of mouth ejecta for staphylococci may provide results representative for carriage sites

Finite temperature quantum simulation of stabilizer Hamiltonians

We present a scheme for robust finite temperature quantum simulation of stabilizer Hamiltonians. The scheme is designed for realization in a physical system consisting of a finite set of neutral atoms trapped in an addressable optical lattice that are controllable via 1- and 2-body operations together with dissipative 1-body operations such as optical pumping. We show that these minimal physical constraints suffice for design of a quantum simulation scheme for any stabilizer Hamiltonian at either finite or zero temperature. We demonstrate the approach with application to the abelian and non-abelian toric codes.Comment: 13 pages, 2 figure

Archival light curves from the Bamberg Sky Patrol - CFOctantis, 1964-1976

We use the archive of the Bamberg Sky Patrol to obtain light curves of the active K subgiant CF Octantis for the interval 1964-1976. Digitised images of the field near CF Oct were obtained with a flat-bed scanner. Aperture photometry was performed of photo-positives of these images. Using a transformation to second order in plate magnitude, and first order in B-V, for 9 field stars for each plate, the B magnitudes of CF Octantis were obtained for just over 350 plates. The estimated precision of an individual determination of the B magnitude of CF Oct is 0.05 mag. Analysis of the resulting data reveals the known 20 d rotational variation of this star, and shows the evolution of the light curves from year to year. We obtain light curves with good phase coverage for 1964 to 1969 inclusive, partial light curves for 1970 and 1976, and a few data points from 1971. The amplitude of variation ranges from ~0.2 to \~0.4 mag. There is evidence that the characteristic rotation period of the star in the 1960s was slightly less than that measured from photoelectric photometry in the 1980s.Comment: 11 pages, 11 figures, accepted for publication in MNRA

Near-Infrared Photometry of Carbon Stars

Near-infrared, JHKL, photometry of 239 Galactic carbon-rich variable stars is presented and discussed. From these and published data the stars were classified as Mira or non-Mira variables and amplitudes and pulsation periods, ranging from 222 to 948 days for the Miras, were determined for most of them. A comparison of the colour and period relations with those of similar stars in the Large Magellanic Cloud indicates minor differences, which may be the consequence of sample selection effects. Apparent bolometric magnitudes were determined by combining the mean JHKL fluxes with mid-infrared photometry from IRAS and MSX. Then, using the Mira period luminosity relation to set the absolute magnitudes, distances were determined -- to greater accuracy than has hitherto been possible for this type of star. Bolometric corrections to the K magnitude were calculated and prescriptions derived for calculating these from various colours. Mass-loss rates were also calculated and compared to values in the literature. Approximately one third of the C-rich Miras and an unknown fraction of the non-Miras exhibit apparently random obscuration events that are reminiscent of the phenomena exhibited by the hydrogen deficient RCB stars. The underlying cause of this is unclear, but it may be that mass loss, and consequently dust formation, is very easily triggered from these very extended atmospheres.Comment: 35 pages, 21 figs, accepted for publication in MNRAS. Large data table will be available on-line onl

Why alternative teenagers self-harm: exploring the link between non-suicidal self-injury, attempted suicide and adolescent identity

Background: The term ‘self-harm’ encompasses both attempted suicide and non-suicidal self-injury (NSSI). Specific adolescent subpopulations such as ethnic or sexual minorities, and more controversially, those who identify as ‘Alternative’ (Goth, Emo) have been proposed as being more likely to self-harm, while other groups such as ‘Jocks’ are linked with protective coping behaviours (for example exercise). NSSI has autonomic (it reduces negative emotions) and social (it communicates distress or facilitates group ‘bonding’) functions. This study explores the links between such aspects of self-harm, primarily NSSI, and youth subculture.<p></p> Methods: An anonymous survey was carried out of 452 15 year old German school students. Measures included: identification with different youth cultures, i.e. Alternative (Goth, Emo, Punk), Nerd (academic) or Jock (athletic); social background, e.g. socioeconomic status; and experience of victimisation. Self-harm (suicide and NSSI) was assessed using Self-harm Behavior Questionnaire and the Functional Assessment of Self-Mutilation (FASM).<p></p> Results: An “Alternative” identity was directly (r ≈ 0.3) and a “Jock” identity inversely (r ≈ -0.1) correlated with self-harm. “Alternative” teenagers self-injured more frequently (NSSI 45.5% vs. 18.8%), repeatedly self-injured, and were 4–8 times more likely to attempt suicide (even after adjusting for social background) than their non-Alternative peers. They were also more likely to self-injure for autonomic, communicative and social reasons than other adolescents.<p></p> Conclusions: About half of ‘Alternative’ adolescents’ self-injure, primarily to regulate emotions and communicate distress. However, a minority self-injure to reinforce their group identity, i.e. ‘To feel more a part of a group’

Leukocytes are primed in peripheral blood for activation during term and preterm labour†

We hypothesized that the priming and activation of maternal leukocytes in peripheral blood is a key component of parturition, and that inappropriate preterm priming of leukocytes might initiate preterm labour and delivery. The purpose of this study was to characterize peripheral blood leukocyte activation during human term and preterm labour. We obtained blood samples from pregnant women at term and preterm, both in labour and not in labour. Leukocytes were characterized according to cell subtype and cell surface marker expression. Additionally, we quantified leukocyte cytokine mRNA production, migratory ability and reactive oxygen species production of neutrophils and macrophages. We found that both term and preterm labour were associated with an increase in monocyte and neutrophil proportion or number—neutrophil migratory ability and cell surface marker expression indicating activation. Messenger RNA expression of IL-1β and IL-8, MCP-1 and TLR-2 was also increased. We conclude that leukocytes in peripheral blood are primed in preparation for activation during term and preterm labour, and that this may contribute to the pathophysiological events of parturition. These data may lead to novel therapies and diagnostic tools for the prevention and/or diagnosis of preterm birth