Metal chelates as anti-cancer agents. II cytotoxic action of palladium and platinum complexes of 6-mercaptopurine and thioguanine.

The metal complexes Pd(MP)2.2H2O, Pt(MP)2H2O (MPH=6-mercaptopurine), Pt(AMP2.3H2O and Pd3(AMP)4Cl2(AMPH).4H2O (AMPH=thioguanine) have been isolated. They were screened for anti-tumour activity in the L-1210 lymphoid leukaemia test system in mice. All 4 show marked anti-tumour activity, the complex Pt(AMP)2.3H2O giving a T/C of 185 at the optimum dosage. However, the anti-tumour activity of the metal complexes is somewhat less than that shown by the parent purines under the same conditions

A Survey of 56 Mid-latitude EGRET Error Boxes for Radio Pulsars

We have conducted a radio pulsar survey of 56 unidentified gamma-ray sources from the 3rd EGRET catalog which are at intermediate Galactic latitudes (5 deg. < |b| < 73 deg.). For each source, four interleaved 35-minute pointings were made with the 13-beam, 1400-MHz multibeam receiver on the Parkes 64-m radio telescope. This covered the 95% error box of each source at a limiting sensitivity of about 0.2 mJy to pulsed radio emission for periods P > 10 ms and dispersion measures < 50 pc cm-3. Roughly half of the unidentified gamma-ray sources at |b| > 5 deg. with no proposed active galactic nucleus counterpart were covered in this survey. We detected nine isolated pulsars and four recycled binary pulsars, with three from each class being new. Timing observations suggest that only one of the pulsars has a spin-down luminosity which is even marginally consistent with the inferred luminosity of its coincident EGRET source. Our results suggest that population models, which include the Gould belt as a component, overestimate the number of isolated pulsars among the mid-latitude Galactic gamma-ray sources and that it is unlikely that Gould belt pulsars make up the majority of these sources. However, the possibility of steep pulsar radio spectra and the confusion of terrestrial radio interference with long-period pulsars (P > 200 ms) having very low dispersion measures (< 10 pc cm-3, expected for sources at a distance of less than about 1 kpc) prevent us from strongly ruling out this hypothesis. Our results also do not support the hypothesis that millisecond pulsars make up the majority of these sources. Non-pulsar source classes should therefore be further investigated as possible counterparts to the unidentified EGRET sources at intermediate Galactic latitudes.Comment: 24 pages, including 4 figures and 3 tables. Accepted for publication in Ap

Within-species variation in OMV cargo proteins:The Myxococcus xanthus OMV pan-proteome

Extracellular membrane vesicles are produced by all domains of life (bacteria, archaea and eukaryotes). Bacterial extracellular vesicles (outer membrane vesicles or OMVs) are produced by outer membrane blebbing, and contain proteins, nucleic acids, virulence factors, lipids and metabolites. OMV functions depend on their internal composition, therefore understanding the proteome of OMVs, and how it varies between organisms, is imperative. Here, we report a comparative proteomic profiling of OMVs from strains of Myxococcus xanthus, a predatory species of Gram-negative myxobacteria whose secretions include secondary metabolites and hydrolytic enzymes, thought to be involved in prey lysis. Ten strains were chosen for study, of which seven had genome sequences available. The remaining three strains were genome sequenced allowing definition of the core and accessory genes and genome-derived proteins found within the pan-genome and pan-proteome respectively. OMVs were isolated from each strain and proteins identified using mass spectrometry. The M. xanthus OMV pan-proteome was found to contain tens of ‘core’ and hundreds of ‘accessory’ proteins. Properties of the OMV pan-proteome were compared with those of the pan-proteome deduced from the M. xanthus pan-genome. On average, 80% of ‘core’ OMV proteins are encoded by genes of the core genome, yet the OMV proteomes of individual strains contain subsets of core genome-derived proteins which only partially overlap. In addition, the distribution of characteristics of vesicle proteins does not correlate with the genome-derived proteome characteristic distribution. We hypothesize that M. xanthus cells package a personalized subset of proteins whose availability is only partially dictated by the presence/absence of encoding genes within the genome

An automated method for mapping geomorphological expressions of former subglacial meltwater pathways (hummock corridors) from high resolution digital elevation data

Elongated tracts of hummocks or ‘hummock corridors’, exposed on palaeo-ice sheet beds, are believed to represent former subglacial meltwater pathways. Here, we present a method, coded in MATLAB, for automatically detecting and mapping hummock corridors from high-resolution digital elevation models (DEMs). Initially the DEM is filtered to remove bed roughness outside the size range of hummocks. A Fast Fourier Transform is then performed to determine the dominant orientation of hummock corridors and remove misaligned features. Finally, image segmentation is used to isolate and extract the hummock corridors as a binary mask. We tested this automated approach visually and statistically against detailed manual mapping in three areas of Canada and northern Scandinavia. Results show that while the automated method does not perfectly reproduce the manual mapping, it successfully captures the general configuration, morphometry (length, width) and location of hummock corridors, despite variation in expression across and between sites. This technique is ideally suited to take advantage of newly available high-resolution digital elevation data (e.g. the ArcticDEM), whose enormous volume makes large-scale manual mapping prohibitively time consuming. Its application will enable efficient and comprehensive mapping of the spatial distribution of hummock corridors across palaeo-beds that is necessary for deriving insights into their formation and the organisation of subglacial meltwater flow beneath ice sheets

Researching the Ends of Identity: Birth and Death on Social Media

This paper argues that expanding the scope of social media studies to examine birth and early life at one end, and death and memorialisation at the other, demonstrates that social media is never just about an individual, but also the way individuals are always already joined together as families, groups, communities and more. Mapping these ends of identity also reveals more of the nuances of everyday social media use and its impact

The participation paradigm in audience research

As today's media simultaneously converge and diverge, fusing and hybridizing across digital services and platforms, some researchers argue that audiences are dead-long live the user! But for others, it is the complex interweaving of continuities and changes that demands attention, especially now that audiencing has become a vital mode of engaging with all dimensions of daily life. This article asks how we should research audiences in a digital networked age. I argue that, while many avenues are being actively pursued, many researchers are concentrating on the notion of participation, asking, on the one hand, what modes of participation are afforded to people by the particular media and communication infrastructures which mediate social, cultural or political spheres of life? And, on the other hand, how do people engage with, accede to, negotiate or contest this as they explore and invent new ways of connecting with each other through and around media? The features of this emerging participation paradigm of audience research are examined in this article