CD44 expression positively correlates with Foxp3 expression and suppressive function of CD4+ Treg cells

<p>Abstract</p> <p>Background</p> <p>CD4⁺CD25⁺regulatory T (T_reg) cells develop in the thymus and can suppress T cell proliferation, modulated by Foxp3 and cytokines; however, the relevance of CD44 in T_regcell development is less clear. To address this issue, we analyzed Foxp3 expression in CD44⁺T_regcells by using multiple parameters, measured the levels of the immunoregulatory cytokine interleukin (IL)-10 in various thymocyte subsets, and determined the suppressor activity in different splenic T_regcell populations.</p> <p>Results</p> <p>Within mouse thymocytes, we detected T_regcells with two novel phenotypes, namely the CD4⁺CD8^-CD25⁺CD44⁺and CD4⁺CD8^-CD25⁺CD44^-staining features. Additional multi-parameter analyses at the single-cell and molecular levels suggested to us that CD44 expression positively correlated with Foxp3 expression in thymocytes, the production of IL-10, and T_regactivity in splenic CD4⁺CD25⁺T cells. This suppressive effect of T_regcells on T cell proliferation could be blocked by using anti-IL-10 neutralizing antibodies. In addition, CD4⁺CD25⁺CD44⁺T_regcells expressed higher levels of IL-10 and were more potent in suppressing effector T cell proliferation than were CD4⁺CD25⁺CD44^-cells.</p> <p>Conclusion</p> <p>This study indicates the presence of two novel phenotypes of T_regcells in the thymus, the functional relevance of CD44 in defining T_regcell subsets, and the role of both IL-10 and Foxp3 in modulating the function of T_regcells.</p> <p>Reviewers</p> <p>This article was reviewed by Dr. M. Lenardo, Dr. L. Klein & G. Wirnsberger (nominated by Dr. JC Zungia-Pfluker), and Dr. E.M. Shevach.</p

C2H N=1-0 and N2H+ J=1-0 observations of Planck Galactic cold clumps

A survey of C2H N=1-0 and N2H+ J=1-0 toward Planck Galactic cold clumps (PGCCs) was performed using the Purple Mountain Observatory's 13.7 m telescope. C2H and N2H+ were chosen to study the chemical evolutionary states of PGCCs. Among 121 observed molecular cores associated with PGCCs, 71 and 58 are detected with C2H N=1-0 and N2H+ J=1-0, respectively. The detected lines of most sources can be fitted with a single component with compatible Vlsr and line widths, which confirms that these PGCC cores are very cold (with gas temperatures 9-21 K) and quiescent while still dominanted by turbulence. The ratio between the column densities of C2H and N2H+ (N(C2H)/N(N2H+)) is found to be a good tracer for the evolutionary states of PGCC cores. Gas-grain chemical model can reproduce the decreasing trend of N(C2H)/N(N2H+) as a function of time. The cores with the lowest abundances of N2H+ (X[N2H+] < 10^{-10}) are the youngest, and have nearly constant abundances of C2H. In evolved cores with X[N2H+] ~ 1E-9, abundances of C2H drop quickly as the exhaustion of carbon atoms. Although these PGCC cores are in different evolutionary states, they are all quite young ( N(N2H+). Mapping observations are carried out toward 20 PGCC cores. The PGCC cores in Cepheus have lower N(C2H)/N(N2H+) and larger line widths compared with those in Taurus. This implies that PGCC cores in Taurus are less chemically evolved than those in Cepheus.Comment: 23 pages, 6 figures, 5 table

A system-level mechanistic investigation of traditional Chinese medicine, Yinlai Decoction, for related diseases

Purpose: To systemically explore the pharmacological mechanisms of traditional Chinese medicine, Yinlai Decoction (YD), used in the clinical management of pediatric diseases such as pneumonia and recurrent respiratory tract infections.Methods: An ingredient-target-disease database of YD was constructed using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). First, the molecular targets related to lung and stomach diseases were searched and screened to avoid duplication. Second, the associations between these molecular targets were evaluated via Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and Gene Ontology (GO) and Pathway enrichment analysis in STRING.Results: A total of 627 chemical ingredients and 654 protein targets in YD were obtained. After further screening, 38 molecular targets linked to respiratory diseases, inflammatory responses and various infections were identified. Finally, 576 GO terms and 75 KEGG pathway terms were obtained by analyzing gene functional annotation clusters and abundance value of these targets. Most of these terms were closely related to the inflammatory response.Conclusion: Based on these in silico findings, the use of YD for treating respiratory diseases, inflammation and various infections, most probably via the suppression of inflammation, has been established. The approach adopted in this study can serve as a model methodology to develop an innovative TCM candidate drug at a network pharmacology level.Keywords: Yinlai Decoction, Network (System) pharmacology, Inflammation, Interacting genes/proteins, Gene ocntology, Pathway enrichment analysi

Gas emissions in Planck cold dust clumps---A Survey of the J=1-0 Transitions of 12^{12}CO, 13^{13}CO, and C18^{18}O

A survey toward 674 Planck cold clumps of the Early Cold Core Catalogue (ECC) in the J=1-0 transitions of $^{12}$CO, $^{13}$CO and C$^{18}$O has been carried out using the PMO 13.7 m telescope. 673 clumps were detected with the $^{12}$CO and $^{13}$CO, and 68% of the samples have C$^{18}$O emission. Additional velocity components were also identified.A close consistency of the three line peak velocities was revealed for the first time. Kinematic distances are given out for all the velocity components and half of the clumps are located within 0.5 and 1.5 kpc. Excitation temperatures range from 4 to 27 K, slightly larger than those of $T_d$. Line width analysis shows that the majority of ECC clumps are low mass clumps. Column densities N$_{H_{2}}$ span from 10$^{20}$ to 4.5$\times10^{22}$ cm$^{-2}$ with an average value of (4.4$\pm$3.6)$\times10^{21}$ cm$^{-2}$. N$_{H_{2}}$ cumulative fraction distribution deviates from the lognormal distribution, which is attributed to optical depth. The average abundance ratio of the $^{13}$CO to C$^{18}$O in these clumps is 7.0$\pm$3.8, higher than the terrestrial value. Dust and gas are well coupled in 95% of the clumps. Blue profile, red profile and line asymmetry in total was found in less than 10% of the clumps, generally indicating star formation is not developed yet. Ten clumps were mapped. Twelve velocity components and 22 cores were obtained. Their morphologies include extended diffuse, dense isolated, cometary and filament, of which the last is the majority. 20 cores are starless.Only 7 cores seem to be in gravitationally bound state. Planck cold clumps are the most quiescent among the samples of weak-red IRAS, infrared dark clouds, UC H{\sc ii} region candidates, EGOs and methanol maser sources, suggesting that Planck cold clumps have expanded the horizon of cold Astronomy.Comment: Accepted to Ap

A simulation study on the measurement of D0-D0bar mixing parameter y at BES-III

We established a method on measuring the \dzdzb mixing parameter $y$ for BESIII experiment at the BEPCII $e^+e^-$ collider. In this method, the doubly tagged $\psi(3770) \to D^0 \overline{D^0}$ events, with one $D$ decays to CP-eigenstates and the other $D$ decays semileptonically, are used to reconstruct the signals. Since this analysis requires good $e/\pi$ separation, a likelihood approach, which combines the $dE/dx$, time of flight and the electromagnetic shower detectors information, is used for particle identification. We estimate the sensitivity of the measurement of $y$ to be 0.007 based on a $20fb^{-1}$ fully simulated MC sample.Comment: 6 pages, 7 figure

Food Markets with Live Birds as Source of Avian Influenza

A patient may have been infected with highly pathogenic avian influenza virus H5N1 in Guangzhou, People's Republic of China, at a food market that had live birds. Virus genes were detected in 1 of 79 wire cages for birds at 9 markets. One of 110 persons in the poultry business at markets had neutralizing antibody against H5N1.link_to_subscribed_fulltex