Comparison of the volatiles composition between healthy and buprestid infected Juglans regia (Juglandaceae).

Meliboeus ohbayashii primoriensis (Coleoptera: Buprestidae) is an important pest of the walnut tree Juglans regia (Juglandaceae), but the volatiles mediating this plant–herbivore interaction are unknown. In this study, volatiles emitted by healthy J. regia and by plants infested with M. ohbayashii primoriensis (Coleoptera: Buprestidae) were obtained by a dynamic headspace method and analyzed by gas chromatography-mass spectrometry (Shanxi, China). We identified 26 major compounds and compared the volatile composition of healthy and buprestid-infected J. regia. Green leaf volatiles were detected in all damaged plants, including the monoterpenoids β-phellandrene and (E)-β-ocimene, the sesquiterpenoids (-)-β-bourbonene, β-ylangene, and (E,E)-α-farnesene, the alcohols linalool, myrtenol, and (E)-(-)-pinocarveol, the ketones (E)-pinocamphone and (Z)-pinocamphone, and the ester methyl salicylate. The major volatiles detected in healthy plants were β-pinene (36.26 %), α-pinene (23.81 %), D-limonene (12.03 %), sabinene (8.63 %), and β-myrcene (4.35 %). The main volatiles from M. ohbayashii primoriensis larva-infested plants were β-pinene (37.82 %), α-pinene (20.36 %), D-limonene (14.71 %), germacrene D (5.24 %), sabinene (4.52 %), and β-phellandrene (3.80 %). These results enrich our understanding of volatiles of healthy plants and plants infested with M. ohbayashii primoriensis. Furthermore, they provide a theoretical basis and scientific foundation for integrated pest management and for effective ecologically sustainable pest control strategie

Comparación de la composición de los volátiles de Juglans regia (Juglandaceae) sanos e infestadas por un escarabajo bupréstido

Meliboeus ohbayashii primoriensis (Coleoptera: Buprestidae) is an important pest of the walnut tree Juglans regia (Juglandaceae), but the volatiles mediating this plant–herbivore interaction are unknown. In this study, volatiles emitted by healthy J. regia and by plants infested with M. ohbayashii primoriensis (Coleoptera: Buprestidae) were obtained by a dynamic headspace method and analyzed by gas chromatography-mass spectrometry (Shanxi, China). We identified 26 major compounds and compared the volatile composition of healthy and buprestid-infected J. regia. Green leaf volatiles were detected in all damaged plants, including the monoterpenoids β-phellandrene and (E)-β-ocimene, the sesquiterpenoids (-)-β-bourbonene, β-ylangene, and (E,E)-α-farnesene, the alcohols linalool, myrtenol, and (E)-(-)-pinocarveol, the ketones (E)-pinocamphone and (Z)-pinocamphone, and the ester methyl salicylate. The major volatiles detected in healthy plants were β-pinene (36.26 %), α-pinene (23.81 %), D-limonene (12.03 %), sabinene (8.63 %), and β-myrcene (4.35 %). The main volatiles from M. ohbayashii primoriensis larva-infested plants were β-pinene (37.82 %), α-pinene (20.36 %), D-limonene (14.71 %), germacrene D (5.24 %), sabinene (4.52 %), and β-phellandrene (3.80 %). These results enrich our understanding of volatiles of healthy plants and plants infested with M. ohbayashii primoriensis. Furthermore, they provide a theoretical basis and scientific foundation for integrated pest management and for effective ecologically sustainable pest control strategies.Meliboeus ohbayashii primoriensis (Coleoptera: Buprestidae) es una plaga importante del nogal Juglans regia (Juglandaceae), pero se desconocen los compuestos volátiles que median esta interacción planta-herbívoro. En este estudio, se obtuvieron los volátiles emitidos por plantas de J. regia tanto sanas como infestadas con M. ohbayashii primoriensis (Coleoptera: Buprestidae), mediante un método de espacio de cabeza dinámico y se analizaron por cromatografía de gases-espectrometría de masas (Shanxi, China). Se identificaron 26 compuestos principales y se comparó la composición volátil de J. regia sana e infectada con bupréstido. Se detectaron volátiles de hojas verdes en todas las plantas dañadas, incluidos los monoterpenoides β-felandreno y (E)-β-ocimeno, los sesquiterpenoides (-)-β-bourboneno, β-ylangeno y (E,E)-α-farnesano, los alcoholes linalool, mirtenol y (E)-(-)-pinocarveol, las cetonas (E)-pinocamfono y (Z)-pinocamfono, y el éster salicilato de metilo. Los principales volátiles detectados en plantas sanas fueron β-pineno (36,26 %), α-pineno (23,81 %), D-limoneno (12,03 %), sabineno (8,63 %) y β-mirceno (4,35 %). Los principales volátiles de las plantas infestadas de larvas de M. ohbayashii primoriensis fueron β-pineno (37,82 %), α-pineno (20,36 %), D-limoneno (14,71 %), germacreno D (5,24 %), sabineno (4,52 %), y β-felandreno (3,80 %). Estos resultados enriquecen nuestra comprensión de los volátiles de plantas sanas e infestadas con M. ohbayashii primoriensis. Además, proporcionan una base teórica y una científica para el manejo integrado de plagas y para estrategias efectivas, ecológicamente sostenibles, para el control de plagas