The subject of the parliamentary investigation

The article examines the subject of the parliamentary investigations. A comparative legal analysis of the subject of the parliamentary investigations in foreign countries is carried out. The examples of the parliamentary investigations are given

Uticaj vrste klona prokupca i postupka vinifikacije na sadržaj rezveratrola u vinu

The focus of this study was to investigate the effects of clones (subvarieties) of autochthonous Serbian grape variety Prokupac, along with the influence of vinification method on the content of transand cis-resveratrol as well as on total phenolic content (TPC) in wines. Wines were made from four clones of Prokupac variety (PR1, 40/1, PR6 and PR7) by application of different periods of maceration duration (1, 5 and 10 days). The effects of different species of selected wine yeasts and glucosidase enzymes on the quantity of resveratrol and TPC in wine made from PR6 variety were also investigated. The content of trans-resveratrol varied from 0.27 mg/L to 1.46 mg/L. The highest content of resveratrol was determined in Prokupac clone PR6, and the lowest in PR7 clone. An increase in resveratrol and TPC content was observed in all clones when the duration of maceration was prolonged. Wine produced by application of b enzyme preparation and 299 yeast had the highest concentration of total resveratrol (4.23 mg/L). The TPC was the highest in the wine made by combined application of yeast 299 and OE enzyme. The obtained results showed that by adequate selection of varieties, prolonged duration of maceration, application of appropriate species of yeast and enzyme preparations, it is possible to increase the content of resveratrol and other phenolic compounds in wine.Cilj ovog istraživanja bio je da se ispita uticaj različitih klonova autohtone srpske sorte Prokupac kao i postupak vinifikacije na sadržaj transi cisrezveratrola i sadržaj ukupnih polifenola (SUP) u vinima. Vina su proizvedena od četiri klona sorte Prokupac (PR1, 40/1, PR6 i PR7) primenom različitih dužina trajanja maceracije (1, 5 i 10 dana) za svaki klon. Takođe je ispitivan uticaj različitih izabranih kvasaca i enzimskih preparata glikozidaza na sadržaj rezveratrola i SUP u vinima proizvedenim od klona PR6. Sadržaj transi cisrezveratrola je određen HPLC metodom sa UV detektorom uz prethodnu tečno čvrstu ekstrakciju (SPE). Određivanje SUP je urađeno metodom po FolinČokalteu. Sadržaj transrezveratrola je bio od 0,27 mg/L do 1,46 mg/L. Najviši sadržaj rezveratrola je bio u klonu PR6 dok je najniži PR7. Povećanje sadržaja rezveratrola i SUP je primećeno kod svih klonova kada je maceracija duže trajala. Vino proizvedeno primenom enzimskog preparata glikozidaza b i kvasca 299 je imalo najviši sadržaj ukupnog rezvertrola (4,23 mg/L). Najviši SUP je bio u vinima proizvedenim primenom enzimskog preparata glikozidaza OE i kvasca 299. Dobijeni rezultati ukazuju da je izborom odgovarajućeg klona, dužine maceracije, primene odgovarajućeg kvasca i enzimskog preparata moguće povećati sadržaj rezveratrola i drugih fenolnih jedinjenja u vinu

Скрининг, идентификация и характеристика бактерий Arthrobacter species, продуцирующих внеклеточную β-галактозидазу

β-Галактозидаза прокариот и, в частности, бактерий рода Arthrobacter – внутриклеточный фермент. Штамм Arthrobacter sp. БИМ B-2242 идентифицирован как A. sulfonivorans на основании анализа нуклеотидных последовательностей гена 16S рРНК. Штамм относится к грамположительным неподвижным бактериям с циклом морфогенеза клеток кокк – палочка – кокк, облигатным аэробам, мезофилам, положительным по признаку синтеза каталазы, протеазы, амилазы, уреазы и липазы. A. sulfonivorans ассимилирует лактозу, глюкозу, галактозу, рамнозу, сахарозу, арабинозу, сорбитол, пектин, крахмал в качестве источников углерода и энергии. Штамм утилизирует следуюшие источники азота: дрожжевой экстракт, пептон, бактопептон, триптон, желатин, казеин, казеинат натрия, мочевину, аминонокислоты, аммонийные соли неорганических кислот и нитраты. A. sulfonivorans продуцирует 2 изоформы истинно внеклеточных β-галактозидаз. Неочищенная β-галактозидаза проявляет максимум активности при температуре 42,5°C и pH 7,0, гидролизуя o-нитрофенил-β-D-галактопиранозид (oNPG). Удельная активность внеклеточной β-галактозидазы составила 101,2 ед/мг белка, что в 675,3 раза больше, чем внутриклеточной (0,15 ед/мг белка)

Identification and Description of a Silicic Volcaniclastic Layer in Gale Crater, Mars, Using Active Neutron Interrogation

The Dynamic Albedo of Neutrons instrument aboard the Mars Science Laboratory rover, Curiosity, has been used to map a stratigraphically conformable layer of high‐SiO² material in Gale crater. Previous work has shown that this material contains tridymite, a high‐temperature/low‐pressure felsic mineral, interpreted to have a volcanic source rock. We describe several characteristics including orientation, extent, hydration, and geochemistry, consistent with a volcaniclastic material conformably deposited within a lacustrine mudstone succession. Relationships with widely dispersed alteration features and orbital detections of hydrated SiO² suggest that this high‐SiO² layer extends at least 17 km laterally. Mineralogical abundances previously reported for this high‐SiO² material indicated that hydrous species were restricted to the amorphous (non‐crystalline) fraction, which is dominated by SiO². The low mean bulk hydration of this high‐SiO² layer (1.85 ± 0.13 wt.% water‐equivalent hydrogen) is consistent with silicic glass in addition to opal‐A and opal‐CT. Persistent volcanic glass and tridymite in addition to opal in an ancient sedimentary unit indicates that the conversion to more ordered forms of crystalline SiO² has not proceeded to completion and that this material has had only limited exposure to water since it originally erupted, despite having been transported in a fluviolacustrine system. Our results, including the conformable nature, large areal extent, and presence of volcanic glass, indicate that this high‐SiO² material is derived from the product of evolved magma on Mars. This is the first identification of a silicic volcaniclastic layer on another planet and has important implications for magma evolution mechanisms on single‐plate planets

Hydrogen and chlorine abundances in the Kimberley formation of Gale crater measured by the DAN instrument on board the Mars Science Laboratory Curiosity rover

The Dynamic Albedo of Neutron (DAN) instrument on board the Mars Science Laboratory Curiosity rover acquired a series of measurements as part of an observational campaign of the Kimberley area in Gale crater. These observations were planned to assess the variability of bulk hydrogen and neutron‐absorbing elements, characterized as chlorine‐equivalent concentration, in the geologic members of the Kimberley formation and in surface materials exposed throughout the area. During the traverse of the Kimberley area, Curiosity drove primarily over the “Smooth Hummocky” unit, a unit composed primarily of sand and loose rocks, with occasional stops at bedrock of the Kimberley formation. During the Kimberley campaign, DAN detected ranges of water equivalent hydrogen (WEH) and chlorine‐equivalent concentrations of 1.5–2.5 wt % and 0.6–2 wt %, respectively. Results show that as the traverse progressed, DAN observed an overall decrease in both WEH and chlorine‐equivalent concentration measured over the sand and loose rocks of the Smooth Hummocky unit. DAN measurements of WEH and chlorine‐equivalent concentrations in the well‐exposed sedimentary bedrock of the Kimberley formation show fluctuations with stratigraphic position. The Kimberley campaign also provided an opportunity to compare measurements from DAN with those from the Sample Analysis at Mars (SAM) and the Alpha‐Particle X‐ray Spectrometer (APXS) instruments. DAN measurements obtained near the Windjana drill location show a WEH concentration of ~1.5 wt %, consistent with the concentration of low‐temperature absorbed water measured by SAM for the Windjana drill sample. A comparison between DAN chlorine‐equivalent concentrations measured throughout the Kimberley area and APXS observations of corresponding local surface targets and drill fines shows general agreement between the two instruments

Hydrogen and chlorine abundances in the Kimberley formation of Gale crater measured by the DAN instrument on board the Mars Science Laboratory Curiosity rover

The Dynamic Albedo of Neutron (DAN) instrument on board the Mars Science Laboratory Curiosity rover acquired a series of measurements as part of an observational campaign of the Kimberley area in Gale crater. These observations were planned to assess the variability of bulk hydrogen and neutron‐absorbing elements, characterized as chlorine‐equivalent concentration, in the geologic members of the Kimberley formation and in surface materials exposed throughout the area. During the traverse of the Kimberley area, Curiosity drove primarily over the “Smooth Hummocky” unit, a unit composed primarily of sand and loose rocks, with occasional stops at bedrock of the Kimberley formation. During the Kimberley campaign, DAN detected ranges of water equivalent hydrogen (WEH) and chlorine‐equivalent concentrations of 1.5–2.5 wt % and 0.6–2 wt %, respectively. Results show that as the traverse progressed, DAN observed an overall decrease in both WEH and chlorine‐equivalent concentration measured over the sand and loose rocks of the Smooth Hummocky unit. DAN measurements of WEH and chlorine‐equivalent concentrations in the well‐exposed sedimentary bedrock of the Kimberley formation show fluctuations with stratigraphic position. The Kimberley campaign also provided an opportunity to compare measurements from DAN with those from the Sample Analysis at Mars (SAM) and the Alpha‐Particle X‐ray Spectrometer (APXS) instruments. DAN measurements obtained near the Windjana drill location show a WEH concentration of ~1.5 wt %, consistent with the concentration of low‐temperature absorbed water measured by SAM for the Windjana drill sample. A comparison between DAN chlorine‐equivalent concentrations measured throughout the Kimberley area and APXS observations of corresponding local surface targets and drill fines shows general agreement between the two instruments

Моделирование микрополосковой антенны радиовысотомера для летательного аппарата

The purpose of the article is to carry out mathematical modeling of the main characteristics and parameters of a microstrip antenna, the dielectric substrate of which is created from a domestic ceramic sample and to substantiate the use of a microstrip antenna as a transmitting and receiving antenna in a radio altimeter. It is shown that a microstrip antenna on a ceramic material substrate with a relative permittivity ε = 9.6 and a thickness of 0.2 mm has good electrical parameters in the frequency range of 31.5–33.5 GHz. Including the width of the antenna radiation pattern at the level of –3 dB is 12.5 degrees in the horizontal plane and 26.7 degrees in the vertical plane. In this case, the antenna gain is 17 dB and the level of the first side lobe is –20 dB relative to the main antenna lobe. Also the practical application of microstrip array antennas inphase as a transmitting and receiving antenna for a radio altimeter installed on aircraft is described in the article. The range of the altimeter reaches 300 m.Целью статьи является проведение математического моделирования основных характеристик и параметров микрополосковой антенны, диэлектрическая подложка которой создана из отечественного образца керамики, и обоснование применения микрополосковой антенны в качестве приемо-передающей антенны в радиовысотомере. Показано, что микрополосковая антенна имеет хорошие электрические параметры в частотном диапазоне 31,5–33,5 ГГц в случае размещения ее на подложке из керамического материала с относительной диэлектрической проницаемостью ε = 9,6 и толщиной 0,2 мм. В том числе ширина диаграммы направленности антенны по уровню –3 дБ составляет 12,5 град в горизонтальной плоскости и 26,7 град в вертикальной плоскости. При этом коэффициент усиления антенны равен 17 дБ, а уровень первого бокового лепестка составляет –20 дБ относительно главного лепестка антенны. Также в статье предложено практическое применение антенной решетки синфазных микрополосковых антенн в качестве приемо-передающей антенны радиовысотомера, устанавливаемого на летательные аппараты. Дальность действия высотомера достигает 300 м

Методические подходы к валидации технологических процессов получения терапевтических рекомбинатных белков на основе концепции «Quality by Design»

Validation of production processes based on the Quality by Design (QbD) principles calls for thorough scientific understanding of the processes and enhancement of their stability by implementation of new technologies. The aim of the study consisted in substantiating a QbD-based technological approach to validation of commercial production of dornase alfa. For this purpose a design space was established in a scale-down model, i.e. 2 L reactors; the model was shown to be representative in terms of all parameters except for the reactor size; the similarity of hydrodynamic conditions, design characteristics and operation modes of laboratory, pilot and commercial scale reactors was established; the process scalability was demonstrated by using the PCA (Principal Component Analysis) multivariate mathematical model including the volume range of 2–1000 L, input and output process parameters and product quality attributes for a number of recombinant therapeutic products derived from the same CHO cell line and expression construction as dornase alfa producer. The article demonstrates the applicability of engineering space, which includes bioreactor design features and production process parameters, to different production scales by implementing 3 processes at the pilot scale (100 L) and 2 processes at the commercial scale (1000 L) and building a PCA model based on the obtained data.Валидация технологических процессов на основе концепции «качество, встроенное при разработке» (Quality by Design, QbD) требует глубокого научного понимания процессов и повышения их устойчивости путем внедрения новых технологий. Цель данной работы заключалась в обосновании методологического подхода на основе концепции QbD к валидации промышленного производства активной фармацевтической субстанции (АФС) дорназы альфа. Для этого было определено технологическое пространство процесса в демасштабированной модели — реакторах объемом 2 л; доказана репрезентативность данной модели по независимым от масштаба реактора параметрам; установлено сходство гидродинамических условий, конструкционных особенностей и режимов работы реакторов лабораторных, опытно-промышленных и промышленного объемов; показана масштабируемость процесса посредством демонстрации многовариантной математической модели РСА (Principal Component Analysis), перекрывающей объемы 2–1000 л, включающей входные, выходные параметры процесса и параметры качества продукта для ряда продуцентов рекомбинантных терапевтических белков, созданных на основе той же клеточной линии CHO и экспрессионной конструкции, что и продуцент дорназы альфа. Обоснована применимость инженерного пространства, которое определяется сочетанием конструкционных особенностей биореакторов и технологических параметров процесса, к различным масштабам путем проведения трех процессов в опытно-промышленном масштабе 100 л и двух процессов в промышленном масштабе 1000 л и построении на основе полученных данных модели РСА