IDENTIFIKASI AKTINOMISETES SEDIMEN AIR TAWAR MAMASA, SULAWESI BARAT DAN AKTIVITASNYA SEBAGAI ANTIBAKTERI DAN PELARUT FOSFAT

Identification of Actinomycetes in Freshwater Sediments from Mamasa, West Sulawesi and Their Antibacterial and Phosphate Solubilizing ActivitiesABSTRACTA large number of actinomycetes that have been isolated and screened were obtained from soil and marine samples. Consequently, the possibility of isolating novel Actinomycetes and secondary metabolites compounds strains from soil and marine samples have limited. Exploration of actinomycetes from freshwater sediment is rare. In this study, 30 isolates of Actinomycetes from freshwater sediments in Mamasa District, West Sulawesi were isolated, identified, and screened for their antibacterial and phosphate solubilizing activity. Actinomycetes were isolated by serial dilution method and were identified based on morphological and 16S rRNA gene sequence. Antibiotic activity was screened using the agar plug diffusion method, while soluble phosphate ability was observed by clear zone ratio in PKA medium. Most of the isolates belong to the genus Streptomyces (80%). Out of 30 isolates, 56.6% showed antibacterial activity and 36.6% had potential as solubilizing phosphate which belong to genus Streptomyces, Actinomadura, and Kitasatospora.Keywords: 16S rRNA, Actinomycetes, antibacterial, freshwater sediment, phosphate solubilizing ABSTRAKSebagian besar aktinomisetes yang telah diisolasi dan dilakukan penapisan metabolit sekundernya berasal dari sampel tanah dan laut. Konsekuensinya, kesempatan untuk menemukan aktinomisetes jenis baru maupun yang menghasilkan metabolit sekunder baru dari tanah dan laut semakin berkurang. Eksplorasi aktinomisetes dari lingkungan lain seperti sedimen air tawar jarang dilakukan. Pada penelitian ini, 30 isolat aktinomisetes yang diisolasi dari sedimen air tawar di Kabupaten Mamasa, Sulawesi Barat, telah diidentifikasi dan dilakukan penapisan antibakteri dan kemampuan isolat dalam melarutkan fosfat. Aktinomisetes diisolasi dengan metode pengenceran secara langsung dan selanjutnya diidentifikasi secara morfologi dan molekular berdasarkan gen 16S rRNA. Metode yang digunakan dalam penapisan aktivitas antibakteri adalah agar plug diffusion method, sedangkan kemampuan aktinomisetes dalam melarutkan fosfat diuji dengan cara menumbuhkan isolat pada media PKA. Isolat yang paling banyak diisolasi termasuk ke dalam marga Streptomyces (80%). Dari 30 isolat, 56,6% isolat menunjukkan adanya aktivitas antibakteri dan 36,6% dari isolat berpotensi sebagai pelarut fosfat, yang termasuk ke dalam marga Streptomyces, Actinomadura, dan Kitasatospora.Kata Kunci: 16S rRNA, Aktinomisetes, sedimen air tawar, antibakteri, pelarut fosfa

Effect of urease enzyme and clay mixture in shear strength properties of sand

The utilization of environmentally friendly materials in soil stabilization has grown in the last decade. Recent studies have shown that adding urease enzyme in calcium chloride and urea solution will improve the shear strength properties of sand. The purpose of this paper is to introduce the usage of clay to improve the performance of calcite produced in the mixture of urease enzyme and calcium chloride-urea solution. This mixture is then mixed manually with the clay-sand mixture and its effect is further tested using a direct shear test conducted at every two weeks of curing. The test is carried out to determine the development of the shear strength properties of the stabilized sand. The results from the test show that there is a rise in the cohesion value of the sand due to the addition of the clay mixture

SARS-COV-2 (COVID-19) EXAMINATION USES ANTIGEN SWAB FOR COMPANY’S EMPLOYEES IN SURABAYA ON JULY – AUGUST 2021

Background of the problem: Since 2020, all the countries in the world has been rocked by Covid-19 (Sars-Cov-2) pandemic. Since July 2021, Indonesia became Covid-19 epicentrum with the highest mortality 2.048 people in the world on 10th August 2021. Covid-19 supporting diagnosis checkup uses RT-PCR and Antigen rapid test, this was based on the Indonesian Health Minister instruction about the uses of antigen test for 2019 Corona Virus Disease checkup. Method: This research uses cross sectional, retrospective analytic study by seeing the data that was taken from SARS-Cov-2 antigen rapid test from some location of companies in Surabaya. The swab data is from Sentra Medika Clinic Surabaya by using nasopharyngeal and nasal antigen SARS-CoV-2 rapid test kit (immunochromatography method). Result: The main data of labor’s characteristics is 6033 people. The highest number was on July to August 2021 on the age range of 30-39 years old (39,70%), meanwhile at the age of 60-69 years old is 0,45%. In the case of Covid-19 virus infection, the highest number of positive antigen was at the age range of 30-39 years old was 64 people (41,30%), whereas 2 people (1,30%) were at the age range of 60-69 years old. At the age more than 60, the number of case is not many but the statistic analytic result shows that age influences P0,05 (P 0,260) Covid-19 Antigen swab test result. The case spike happened on July 2021, 153 people were positive of Antigen swab test: 141 people were men (92,16%) and 12 people were women (7,84%). On August 2021, 2 men were positive (100%). Conclusion: The result of the research in July 2021 shows that the highest numbers of labor are at the age of 30-39 years old. The risk of Covid-19 infection mostly at men. Whereas Covid-19 infection risk is at the age of 60-69 years old are in small amount. Age influences the result of Covid-19 Antigen swab test. Meanwhile, gender does not influence the result of Covid-19 Antigen swab test

Positioning Dan Segmentasi Pasar Produk Sarang Infus (Sarung Tangan Infus) Di Rumah Sakit Persyarikatan Muhammadiyah Daerah Surabaya-Sidoarjo

ABSTRACT  Occurrence at risk of infection, risk of injury and stress of hospitalization are three events that often occur in children who are hospitalized with infusion. During this time the efforts of these events have only been overcome by the installation of a buffer on the hand and fixation with gauze rolls that are looped on the child's arm only so that this can only reduce the risk of infection. Provision of infusion nest products (infusion gloves) is an effort to prevent and reduce the occurrence of these three events. Infusion nest products are creative innovations that have been made because so far there are no similar products in hospitals.Marketing research on consumers of nest infusion products is carried out to determine the potential of the market and the behaviors that develop in consumers so that clear information about the customers of infusion nest products can be obtained, especially in hospitals that are sheltered by Muhammadiyah Hospital in Surabaya-Sidoarjo. One of the important data to analyze is data about certain segments of the customer and how the infusion nest producers are able to place their product position on consumer perceptions. This is very important to determine the marketing strategy. appropriate.Customers from hospitals that are in large numbers in Surabaya and scattered are certainly not possible to analyze all, so we need a marketing research procedure by taking samples. samples designed to obtain data on demographic characteristics, psychography, behavior and consumer preferences. In addition, information about perceptual maping from consumers on infusion nest products is needed to find out the map of respondents' perceptions of the nest infusion product attributes. From consumer preference data on marketing attributes of nest infusion products customer segmentation can be done to obtain information about groups of customers based on the choice of the most important attributes. In addition, each segment can be described the characteristics of psychography, demographics and behavior to see the characteristics of each segment. The data about positioning provides information on product position in customer perceptions based on marketing attributes that are superior to infusion nest products.From the data obtained from filling out the questionnaire after validation through Focus Group Discussion with child nurse nurses in two Muhammadiyah Hospital (RS) namely Siti Khodijah Hospital Along and RSIA Aisyiah Pacarkeling Surabaya it was found that infusion nest customers, can be grouped into 2 groups with preference characteristics, demographics, psychography, and different behaviors. While the positioning of infusion nest products in consumer perceptions is still relatively close to a number of superior attributes, such as there are no similar products on the market, products are comfortable for children, products can make it easier for nurses to observe infusion conditions in accordance with hospital accreditation but prices offered by producers can increase the cost of installing IVs so that they can only be temporarily reached by patient patients non BPJS users.Keywords                   : Infusion nest products, positioningCorrespondence to       : [email protected] ABSTRAK  Kejadian Resiko Infeksi, resiko cidera dan stress hospitalisasi merupakan tiga kejadian yang seringkali terjadi pada anak yang mengalami rawat inap yang terpasang infus. Selama ini upaya kejadian tersebut hanya diatasi dengan pemasangan penyangga pada tangan dan fiksasi dengan kasa gulung yang dilingkarkan pada lengan anak saja sehingga hal ini hanya dapat mengurangi kejadian resiko infeksi saja. Pemberian produk sarang infus (sarung tangan infus) merupakan upaya untuk mencegah dan mengurangi terjadinya  ketiga kejadian tersebut. Produk sarang infus merupakan inovasi kreatif yang telah dibuat karena selama ini tidak ada produk serupa yang ada di rumah sakit.Riset pemasaran terhadap konsumen produk sarang infus dilakukan untuk mengetahui potensi – potensi pasar dan prilaku – prilaku yang berkembang pada konsumen sehingga dapat diperoleh informasi yang jelas tentang pelanggan produk sarang infus khususnya di Rumah Sakit yang bernaung pada persyarikatan Muhammadiyah di Surabaya-Sidoarjo. Salah satu data yang penting untuk di analisis adalah data tentang segmen – segmen tertentu dari pelanggan serta bagaimana produsen sarang infus tersebut mampu menempatkan posisi produknya pada persepsi konsumen. Hal ini sangat penting untuk menentukan strategi pemasaran. yang sesuai. Pelanggan dari rumah sakit yang jumlahnya banyak di Surabaya dan terpencar – pencar tentunya tidak mungkin kita analisis semua, sehingga diperlukan prosedur riset pemasaran dengan melakukan pengambilan sampel. sampel yang didesain untuk men -dapatkan data tentang karakteristik demografi, psikografi, prilaku dan preferensi konsumen. Selain itu informasi tentang perceptual maping dari konsumen terhadap produk sarang infus sangat diperlukan untuk mengetahui peta persepsi responden terhadap atribut produk sarang infus. Dari data preferensi konsumen terhadap atribut pemasaran produk sarang infus dapat dilakukan segmentasi pelanggan untuk memperoleh informasi tentang kelompok – kelompok pelanggan berdasarkan pilihan terhadap atribut yang paling dipentingkan. Selain itu masing – masing segmen dapat dideskripsikan karakteristik psikografi, demografi dan prilakunya untuk melihat ciri – ciri yang dimiliki masing – masing segmen. Adapun data tentang positioning memberikan informasi posisi produk dalam persepsi pelanggan berdasarkan atribut pemasaran yang diunggulkan pleh produk sarang infus.Dari data yang diperoleh dari pengisian kuisioner setelah validasi melalui Fokus Group Discussion dengan perawat perawat anak di dua Rumah sakit (RS) Muhammadiyah yaitu RS Siti Khodijah Sepanjang dan RSIA Aisyiah Pacarkeling Surabaya didapatkan bahwa pelanggan sarang infus, dapat dikelompokkan menjadi 2 kelompok dengan karakteristik preferensi, demografi, psikografi, dan prilaku yang berbeda – beda. Sedangkan positioning produk sarang infus dalam persepsi konsumen masih  relatif berdekatan untuk beberapa atribut yang diunggulkan, seperti belum ada produk serupa di pasar, produk nyaman dipakai oleh anak-anak, produk dapat memudahkan perawat untuk mengobservasi kondisi infus sesuai dengan tujuan akreditasi rumah sakit namun harga yang ditawarkan produsen dapat meningkatkan biaya pasang infus sehingga untuk sementara hanya dapat dijangkau oleh pasien pasien non pengguna BPJS.Kata kunci                  : produk sarang infus, positioning,segmentasiKorespondensi             : [email protected]

Effect of urease enzyme and clay mixture in shear strength properties of sand

The utilization of environmentally friendly materials in soil stabilization has grown in the last decade. Recent studies have shown that adding urease enzyme in calcium chloride and urea solution will improve the shear strength properties of sand. The purpose of this paper is to introduce the usage of clay to improve the performance of calcite produced in the mixture of urease enzyme and calcium chloride-urea solution. This mixture is then mixed manually with the clay-sand mixture and its effect is further tested using a direct shear test conducted at every two weeks of curing. The test is carried out to determine the development of the shear strength properties of the stabilized sand. The results from the test show that there is a rise in the cohesion value of the sand due to the addition of the clay mixture

Screening and activity of yeast-associated with cocoa-bean fermentation against phytopathogenic yeast and fungi

Cocoa-bean fermentation has been associated with the involvement of diverse microbial assemblages which consist of a wide array of bacteria and yeast. We attempted to screen and to identify the potential antifungal yeast from this assemblage against phytopathogenic fungi. We employed in-vitro antagonism assay using agar plug methods to performpreliminary screening from 35 yeast isolates followed by total protein production and measurement with Bradford methods. We found three yeast strains that were effective against Trichoderma sp. T009, and two moulds associated with cocoa (Penicilliumsp. Cocoa2 and Fusariumsp.Cocoa 1). The three bioactive yeast strains were identified as Saccharomyces cerevisiae IDI-002, Hanseniasporauvarum IDE-056 and Hanseniasporauvarum IDE-271 based on molecular identification and phylogenetic analysis. The production of antifungal protein from pure cultures on YEPG media resulted intotal protein concentration between 6.20 - 8.17 mg/L. Cell suspension showed higherinhibitory activity compared to thecell-free supernatant gave indication that antifungal proteins in the bulk fermentation was below the minimal inhibitory concentration to cause the inhibitory effect. Further characterization, purification, and optimization are still needed before the up-scale production of antifungal metabolites and its biological control application

SARS-COV-2 (COVID-19) Examination Uses Antigen Swab For Company’s Employees In Surabaya On July – August 2021

Background of the problem: Since 2020, all the countries in the world has been rocked by Covid-19 (Sars-Cov-2) pandemic. Since July 2021, Indonesia became Covid-19 epicentrum with the highest mortality 2.048 people in the world on 10th August 2021. Covid-19 supporting diagnosis checkup uses RT-PCR and Antigen rapid test, this was based on the Indonesian Health Minister instruction about the uses of antigen test for 2019 Corona Virus Disease checkup. Method: This research uses cross sectional, retrospective analytic study by seeing the data that was taken from SARS-Cov-2 antigen rapid test from some location of companies in Surabaya. The swab data is from Sentra Medika Clinic Surabaya by using nasopharyngeal and nasal antigen SARS-CoV-2 rapid test kit (immunochromatography method). Result: The main data of labor’s characteristics is 6033 people. The highest number was on July to August 2021 on the age range of 30-39 years old (39,70%), meanwhile at the age of 60-69 years old is 0,45%. In the case of Covid-19 virus infection, the highest number of positive antigen was at the age range of 30-39 years old was 64 people (41,30%), whereas 2 people (1,30%) were at the age range of 60-69 years old. At the age more than 60, the number of case is not many but the statistic analytic result shows that age influences P0,05 (P 0,260) Covid-19 Antigen swab test result. The case spike happened on July 2021, 153 people were positive of Antigen swab test: 141 people were men (92,16%) and 12 people were women (7,84%). On August 2021, 2 men were positive (100%). Conclusion: The result of the research in July 2021 shows that the highest numbers of labor are at the age of 30-39 years old. The risk of Covid-19 infection mostly at men. Whereas Covid-19 infection risk is at the age of 60-69 years old are in small amount. Age influences the result of Covid-19 Antigen swab test. Meanwhile, gender does not influence the result of Covid-19 Antigen swab test

XVI.C.1_REGULER 101_GANASARI

Kuliah Kerja Nyata (KKN) Reguler periode 101 Divisi XIV Kelompok C 1 Universitas Ahmad Dahlan tahun akademik 2022/2023, berlokasi di Dusun Ganasari, Kalurahan Banjarasri, Kapanewon Kalibawang, Kabupaten Kulon Progo, Daerah Istimewa Yogyakarta. Sebelum dilakukan penerjunan KKN di Dusun Ganasari mahasiswa telah melakukan survei melalui kegiatan observasi dan wawancara terhadap Dukuh Ganasari. Dari hasil survei yang telah dilakukan adapun permasalahan�permasalahan antara lain, masih kurangnya kesadaran warga untuk memilah sampah ditingkat rumah tangga, masih kurangnya pemahaman warga mengenai cara pengelolahan sampah dengan baik dan masih mengatasi pembuangan sampah dengan cara membakar, menimbun serta masih tercampur antara organik dan anorganik