Relevance of pseudospin symmetry in proton-nucleus scattering

The manifestation of pseudospin-symmetry in proton-nucleus scattering is discussed. Constraints on the pseudospin-symmetry violating scattering amplitude are given which require as input cross section and polarization data, but no measurements of the spin rotation function. Application of these constraints to p-58Ni and p-208Pb scattering data in the laboratory energy range of 200 MeV to 800 MeV, reveals a significant violation of the symmetry at lower energies and a weak one at higher energies. Using a schematic model within the Dirac phenomenology, the role of the Coulomb potential in proton-nucleus scattering with regard to pseudospin symmetry is studied. Our results indicate that the existence of pseudospin-symmetry in proton-nucleus scattering is questionable in the whole energy region considered and that the violation of this symmetry stems from the long range nature of the Coulomb interaction.Comment: 22 pages including 9 figures, correction of 1 reference, revision of abstract and major modification of chapter 4, Fig. 6, and Fig. 7; addition of Fig. 8 and Fig.

a quasi-experimental study

Funding Information: This study was elaborated based on the work of Miguelhete Lisboa doctoral program, a Fundação Calouste Gulbenkian scholarship holder, and used grants obtained from The Special Programme for Research and Training in Tropical Diseases (TDR) and co-sponsored by the United Nations Children’s Fund (UNICEF), United Nations Development Programme (UNDP), World Bank, and World Health Organization (WHO)—award ID number: B40151/ 2014. The FCG and WHO/TDR were not involved in the design of the study and collection; analysis and interpretation of data; and writing the manuscript; therefore, the authors are responsible for all information. Publisher Copyright: © 2020 The Author(s).Background In-hospital logistic management barriers (LMB) are considered to be important risk factors for delays in TB diagnosis and treatment initiation (TB-dt), which perpetuates TB transmission and the development of TB morbidity and mortality. We assessed the contribution of hospital auxiliary workers (HAWs) and 24-h TB laboratory services using Xpert (24h-Xpert) on the delays in TB-dt and TB mortality at Beira Central Hospital, Mozambique. Methods A quasi-experimental design was used. Implementation strategy—HAWs and laboratory technicians were selected and trained, accordingly. Interventions—having trained HAW and TB laboratory technicians as expediters of TB LMB issues and assurer of 24h-Xpert, respectively. Implementation outcomes—time from hospital admission to sputum examination results, time from hospital admission to treatment initiation, proportion of same-day TB cases diagnosed, initiated TB treatment, and TB patient with unfavorable outcome after hospitalization (hospital TB mortality). A nonparametric test was used to test the differences between groups and adjusted OR (95% CI) were computed using multivariate logistic regression. Results We recruited 522 TB patients. Median (IQR) age was 34 (16) years, and 52% were from intervention site, 58% males, 60% new case of TB, 12% MDR-TB, 72% TB/HIV co-infected, and 43% on HIV treatment at admission. In the intervention hospital, 93% of patients had same-day TB-dt in comparison with a median (IQR) time of 15 (2) days in the control hospital. TB mortality in the intervention hospital was lower than that in the control hospital (13% vs 49%). TB patients admitted to the intervention hospital were nine times more likely to obtain an early laboratory diagnosis of TB, six times more likely to reduce delays in TB treatment initiation, and eight times less likely to die, when compared to those who were admitted to the control hospital, adjusting for other factors. Conclusion In-hospital delays in TB-dt and high TB mortality in Mozambique are common and probably due, in part, to LMB amenable to poor-quality TB care. Task shifting of TB logistic management services to HAWs and lower laboratory technicians, to ensure 24h-Xpert through “on-the-spot strategy,” may contribute to timely TB detection, proper treatment, and reduction of TB mortality.publishersversionpublishe

Supervised wavelet method to predict patient survival from gene expression data.

In microarray studies, the number of samples is relatively small compared to the number of genes per sample. An important aspect of microarray studies is the prediction of patient survival based on their gene expression profile. This naturally calls for the use of a dimension reduction procedure together with the survival prediction model. In this study, a new method based on combining wavelet approximation coefficients and Cox regression was presented. The proposed method was compared with supervised principal component and supervised partial least squares methods. The different fitted Cox models based on supervised wavelet approximation coefficients, the top number of supervised principal components, and partial least squares components were applied to the data. The results showed that the prediction performance of the Cox model based on supervised wavelet feature extraction was superior to the supervised principal components and partial least squares components. The results suggested the possibility of developing new tools based on wavelets for the dimensionally reduction of microarray data sets in the context of survival analysis

healthcare workers’ perspective from Beira, Mozambique

Funding Information: This study was elaborated based on the work of first Miguelhete Lisboa?s doctoral program, a Funda??o Calouste Gulbenkian (FCG) scholarship holder (ID: P-135647/SBG/2014) and, used grants obtained from World Health Organization, The Special Programme for Research and Training in Tropical Diseases (WHO/TDR) and co-sponsored by the United Nations Children?s Fund, United Nations Development Programme, World Bank and WHO ? award ID number: B40151/2014. The FCG and WHO/TDR were neither involved in the design of the study and collection, analysis, interpretation of data, nor in the writing of manuscript or decision to publish. Therefore, the authors are responsible for all information. The authors acknowledge the Tutorial Commission of the doctoral program of Miguelhete Lisboa (Professors Sonia Dias and Miguel Viveiros at Instituto de Higiene e Medicina Tropical Universidade Nova de Lisboa, Portugal); people who helped in the collection and data management: Marques Nhamonga, Joaquim Lequechane and Estefano Colove; the Centro de Investiga??o Operacional da Beira directorate and all colleagues, the Beira Central Hospital directorate and all healthcare workers. Publisher Copyright: © 2020 Lisboa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Background: Mozambique is one of the countries with the deadly implementation gaps in the tuberculosis (TB) care and services delivery. In-hospital delays in TB diagnosis and treatment, transmission and mortality still persist, in part, due to poor-quality of TB care cascade. Objective: We aimed to assess, from the healthcare workers’ (HCW) perspective, factors associated with poor-quality TB care cascade and explore local sustainable suggestions to improve in-hospital TB management. Methods: In-depth interviews and focus group discussions were conducted with different categories of HCW. Audio-recording and written notes were taken, and content analysis was performed through atlas.ti7. Results: Bottlenecks within hospital TB care cascade, lack of TB staff and task shifting, centralized and limited time of TB laboratory services, and fear of healthcare workers getting infected by TB were mentioned to be the main factors associated with implementation gaps. Interviewees believe that task shifting from nurses to hospital auxiliary workers, and from higher and well-trained to lower HCW are accepted and feasible. The expansion and use of molecular TB diagnostic tools are seen by the interviewees as a proper way to fight effectively against both sensitive and MDR TB. Ensuring provision of N95 respiratory masks is believed to be an essential requirement for effective engagement of the HCW on high-quality in-hospital TB care. For monitoring and evaluation, TB quality improvement teams in each health facility are considered to be an added value. Conclusion: Shortage of resources within the national TB control programme is one of the potential factors for poor-quality of the TB care cascade. Task shifting of TB care and services delivery, decentralization of the molecular TB diagnostic tools, and regular provision of N95 respiratory masks should contribute not just to reduce the impact of resource scarceness, but also to ensure proper TB diagnosis and treatment to both sensitive and MDR TB.publishersversionpublishe

Pharmacological characterisation of arthritis induced by Bothrops jararaca venom in rabbits: a positive cross talk between bradykinin, nitric oxide and prostaglandin E2.

BACKGROUND: Our previous results showed that nitric oxide (NO) and bradykinin (BK) mediate the arthritis induced by Bothrops jararaca venom (BjV) in rabbits. In this study, we investigated the contribution of each receptor of BK as well as the inter-relationship between NO and eicosanoids in BjV-induced arthritis. METHODS: The arthritis was induced in rabbits with 16 microg of BjV injected intra-articularly. Prostaglandin E2 (PGE2), thromboxane B2 (TxB2), leukotriene B4 (LTB4) (radioimmunoassay) and nitrite/nitrate concentrations (NO2/NO3) (Griess reaction) were evaluated in the synovial fluid 4 h later. The animals were prior treated with NO synthase inhibitor (L-NAME; 20 mg/kg/day for 14 days), the B2 antagonist of BK (HOE-140) and the B1 antagonist of BK (des-Arg9[Leu8]-bradykinin), both at a dose of 0.3mg/kg, 30 min prior to the venom injection. RESULTS: Data show that L-NAME and HOE-140 treatment were equally able to reduce PGE2 and NO2/NO3 levels without interfering with TxB2 and LTB4 production. On the contrary, the B1 antagonist of BK inhibited TxB2 and LTB4 production, and did not alter PGE2 and NO metabolites levels in the inflamed joint. DISCUSSIONS: The results presented clarify the contribution of the kinin system, mainly through the B2 receptor, to the local inflammatory response induced by BjV, as well as its positive interaction with PGE2 and NO production

Specific heat and magnetic measurements in Nd0.5Sr0.5MnO3, Nd0.5Ca0.5MnO3 and Ho0.5Ca0.5MnO3 samples

We studied the magnetization as a function of temperature and magnetic field in the compounds Nd0.5Sr0.5MnO3, Nd0.5Ca0.5MnO3 and Ho0.5Ca0.5MnO3. It allowed us to identify the ferromagnetic, antiferromagnetic and charge ordering phases in each case. The intrinsic magnetic moments of Nd3+ and Ho3+ ions experienced a short range order at low temperatures. We also did specific heat measurements with applied magnetic fields between 0 and 9 T and temperatures between 2 and 300 K in all three samples. Close to the charge ordering and ferromagnetic transition temperatures the specific heat curves showed peaks superposed to the characteristic response of the lattice oscillations. Below 10 K the specific heat measurements evidenced a Schottky-like anomaly for all samples. However, we could not successfully fit the curves to either a two level nor a distribution of two-level Schottky anomaly. Our results indicated that the peak temperature of the Schottky anomaly was higher in the compounds with narrower conduction band.Comment: submitted to PR

Fractional Synthesis Rates of Individual Proteins in Rat Soleus and Plantaris Muscles.

Differences in the protein composition of fast- and slow-twitch muscle may be maintained by different rates of protein turnover. We investigated protein turnover rates in slow-twitch soleus and fast-twitch plantaris of male Wistar rats (body weight 412 ± 69 g). Animals were assigned to four groups (n = 3, in each), including a control group (0 d) and three groups that received deuterium oxide (D2O) for either 10 days, 20 days or 30 days. D2O administration was initiated by an intraperitoneal injection of 20 μL of 99% D2O-saline per g body weight, and maintained by provision of 4% (v/v) D2O in the drinking water available ad libitum. Soluble proteins from harvested muscles were analysed by liquid chromatography-tandem mass spectrometry and identified against the SwissProt database. The enrichment of D2O and rate constant (k) of protein synthesis was calculated from the abundance of peptide mass isotopomers. The fractional synthesis rate (FSR) of 44 proteins in soleus and 34 proteins in plantaris spanned from 0.58%/day (CO1A1: Collagen alpha-1 chain) to 5.40%/day NDRG2 (N-myc downstream-regulated gene 2 protein). Eight out of 18 proteins identified in both muscles had a different FSR in soleus than in plantaris (p < 0.05)