Relationships of in situ spectral absorption, pigment ratios and environmental parameters for phytoplankton assemblages in coastal waters

In situ optical measurements of spectral absorption and beam attenuation provide information on the fine scale horizontal and vertical variations in phytoplankton pigments and other measures of phytoplankton photophysiology and ecology in coastal waters. Phytoplankton pigment ratios from discrete sample analyses with High Performance Liquid Chromatography were compared to in situ spectral absorption, hydrography, light and nutrients in protected waters in East Sound, Washington and in coastal waters off Oregon. Clear linear relationships were seen between ratios of photoprotective: photosynthetic carotenoids (PPC: PSC) and the shape of the in situ phytoplankton absorption (aph) spectra in East Sound. Linear relationships between PPC: PSC ratios and aph spectra were also found for Oregon coastal waters within groups of samples (grouped by collection date, location and temperature salinity characteristics). Inshore samples showed a similar relationship as East Sound samples. Diatoms were dominant in East Sound and Oregon shelf waters, with prokaryotes and prymnesiophytes important in Oregon waters further offshore. Environmental parameters were associated with variations in PPC: PSC ratios. Light was clearly an important factor in East Sound with a strong positive association seen between PPC: PSC ratios and recent light history. In Oregon waters, PPC: PSC ratios varied with prior light exposure, nutrients and temperature. Light exposure and temperature predicted 61% of the variability in PPC: PSC ratios for samples with low nutrients (dissolved inorganic nitrogen <2 μ M). In situ optical measurements indicated considerable spatial variation in phytoplankton photophysiology and taxonomic composition in the Oregon costal region. Phytoplankton assemblages often had lower PPC: PSC ratios, flatter particle size distributions slopes suggesting greater contributions by large particles, higher chlorophyll a per particle, and more packaging in the nutrient-rich upwelled waters near shore compared to further offshore. These results indicate that in situ measurements of spectral absorption and beam attenuation can predict PPC: PSC ratios and other photophysiological and taxonomic indices in coastal waters. Such data provide high-resolution information on phytoplankton characteristics on the same temporal and spatial scales as physical properties such as temperature and salinity, and offer important insights into light history and the transfer of absorbed light within phytoplankton cells

Copepod life strategy and population viability in response to prey timing and temperature : testing a new model across latitude, time, and the size spectrum

A new model ("Coltrane": Copepod Life-history Traits and Adaptation to Novel Environments) describes environmental controls on copepod populations via (1) phenology and life history and (2) temperature and energy budgets in a unified framework. The model tracks a cohort of copepods spawned on a given date using a set of coupled equations for structural and reserve biomass, developmental stage, and survivorship, similar to many other individual-based models. It then analyzes a family of cases varying spawning date over the year to produce population-level results, and families of cases varying one or more traits to produce community-level results. In an idealized global-scale testbed, the model correctly predicts life strategies in large Calanus spp. ranging from multiple generations per year to multiple years per generation. In a Bering Sea testbed, the model replicates the dramatic variability in the abundance of Calanus glacialis/marshallae observed between warm and cold years of the 2000s, and indicates that prey phenology linked to sea ice is a more important driver than temperature per se. In a Disko Bay, West Greenland testbed, the model predicts the viability of a spectrum of large-copepod strategies from income breeders with a adult size ~100 μgC reproducing once per year through capital breeders with an adult size > 1000 μgC with a multiple-year life cycle. This spectrum corresponds closely to the observed life histories and physiology of local populations of Calanus finmarchicus, C. glacialis, and Calanus hyperboreus. Together, these complementary initial experiments demonstrate that many patterns in copepod community composition and productivity can be predicted from only a few key constraints on the individual energy budget: the total energy available in a given environment per year; the energy and time required to build an adult body; the metabolic and predation penalties for taking too long to reproduce; and the size and temperature dependence of the vital rates involved

Contrastive Decoding: Open-ended Text Generation as Optimization

Likelihood, although useful as a training loss, is a poor search objective for guiding open-ended generation from language models (LMs). Existing generation algorithms must avoid both unlikely strings, which are incoherent, and highly likely ones, which are short and repetitive. We propose contrastive decoding (CD), a more reliable search objective that returns the difference between likelihood under a large LM (called the expert, e.g. OPT-13b) and a small LM (called the amateur, e.g. OPT-125m). CD is inspired by the fact that the failures of larger LMs are even more prevalent in smaller LMs, and that this difference signals exactly which texts should be preferred. CD requires zero training, and produces higher quality text than decoding from the larger LM alone. It also generalizes across model types (OPT and GPT2) and significantly outperforms four strong decoding algorithms in automatic and human evaluations

Spatial and temporal changes in assemblage structure of zooplankton and pelagic fish in the eastern Bering Sea across varying climate conditions

Zooplankton and pelagic fish samples collected on the eastern Bering Sea shelf in late summer 2003-2010 were used to evaluate spatial and temporal changes in the plankton and nekton community structure. The zooplankton were sampled by vertical towing of a Juday net (168 μm mesh) and oblique towing of a Bongo net (505 μm mesh), and pelagic fish were caught by midwater rope trawl. The communities were compared across climate regimes (in relatively warm and cold years), by latitude (in the northern and southern parts of the shelf), and by water depth (in the inner, middle, and outer domains of the shelf). Zooplankton were dominated by the small copepod Oithona spp. in warm years but relatively larger copepods Pseudocalanus spp. and Acartia spp. in cold years. Notably, the large copepod Calanus spp., an important energy-rich prey for fish, were more abundant in cold years than warm years. Age-0 walleye pollock Gadus chalcogrammus were more abundant in warm years, while capelin Mallotus villosus were abundant within cold-year communities over the northern shelf. Latitudinal variations in communities were more prominent in the cold years, particularly in 2007 and 2010. Cross-shelf variations were evident, particularly for large zooplankton and fish, with communities corresponding to specific oceanographic domains. Outer shelf communities varied less than inner and middle shelf communities between warm and cold periods, suggesting that this region may be less impacted by climate variability. An understanding of the overlap of zooplankton (prey) and fish communities within specific shelf regions or climate regimes may provide information for ecosystem-based approaches to fisheries management

Nutrient and Phytoplankton Dynamics on the Inner Shelf of the Eastern Bering Sea

In the Bering Sea, the nitrogen cycle near Nunivak Island is complicated due to limited nutrient replenishment across this broad shelf, and substantial nitrogen loss through sedimentary processes. While diffusion at the inner front may periodically support new production, the inner shelf in this region is generally described as a regenerative system. This study combines hydrographic surveys with measurements of nitrogen assimilation and benthic fluxes to examine nitrogen cycling on the inner shelf, and connectivity between the middle and inner shelves of the southern and central Bering Sea. Results establish the inner shelf as primarily a regenerative system even in spring, although new production can occur at the inner front. Results also identify key processes that influence nutrient supply to the inner shelf and reveal coupling between the middle shelf nutrient pool and production on the inner shelf

Early marine growth in relation to marine-stage survival rates for Alaska sockeye salmon (Oncorhynchus nerka)

We tested the hypothesis that larger juvenile sockeye salmon (Oncorhynchus nerka) in Bristol Bay, Alaska, have higher marine-stage survival rates than smaller juvenile salmon. We used scales from returning adults (33 years of data) and trawl samples of juveniles (n= 3572) collected along the eastern Bering Sea shelf during August through September 2000−02. The size of juvenile sockeye salmon mirrored indices of their marine-stage survival rate (e.g., smaller fish had lower indices of marine-stage survival rate). However, there was no relationship between the size of sockeye salmon after their first year at sea, as estimated from archived scales, and brood-year survival size was relatively uniform over the time series, possibly indicating size-selective mortality on smaller individuals during their marine residence. Variation in size, relative abundance, and marine-stage survival rate of juvenile sockeye salmon is likely related to ocean conditions affecting their early marine migratory pathways along the eastern Bering Sea shelf

Effects of short-term treatment with atorvastatin in smokers with asthma - a randomized controlled trial

&lt;b&gt;Background&lt;/b&gt; The immune modulating properties of statins may benefit smokers with asthma. We tested the hypothesis that short-term treatment with atorvastatin improves lung function or indices of asthma control in smokers with asthma.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Methods&lt;/b&gt; Seventy one smokers with mild to moderate asthma were recruited to a randomized double-blind parallel group trial comparing treatment with atorvastatin (40 mg per day) versus placebo for 4 weeks. After 4 weeks treatment inhaled beclometasone (400 ug per day) was added to both treatment arms for a further 4 weeks. The primary outcome was morning peak expiratory flow after 4 weeks treatment. Secondary outcome measures included indices of asthma control and airway inflammation.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Results&lt;/b&gt; At 4 weeks, there was no improvement in the atorvastatin group compared to the placebo group in morning peak expiratory flow [-10.67 L/min, 95% CI -38.70 to 17.37, p=0.449], but there was an improvement with atorvastatin in asthma quality of life score [0.52, 95% CI 0.17 to 0.87 p=0.005]. There was no significant improvement with atorvastatin and inhaled beclometasone compared to inhaled beclometasone alone in outcome measures at 8 weeks.&lt;p&gt;&lt;/p&gt; &lt;b&gt;Conclusions&lt;/b&gt; Short-term treatment with atorvastatin does not alter lung function but may improve asthma quality of life in smokers with mild to moderate asthma. Clinicaltrials.gov identifier: NCT0046382