109 research outputs found
Improving the indoor thermal environment with ceiling radiant terminals
A CFD (computational Fluid Dynamics) simulation model of the porous ceiling radiant air-conditioning system was established to study the influence of the ceiling temperature and envelope temperature (including the temperature of the walls and the floor of a room) on the thermal environment in the room equipped with such a system. The results showed that, for the summer condition, higher ceiling temperatures would result in higher indoor air temperature and higher Predicted Percentage Dissatisfied (PPD), which meant potential discomfort of occupants in the room. For the winter condition, however, a higher ceiling temperature within 28°C would result in a lower PPD, thus improved the thermal comfort. Considering the energy-conservation, the thermal comfort could be assured if the ceiling temperature was not more than 28°C. As for the effect of envelope temperature, the result showed that the increase in the envelope temperature during summer could result in a higher indoor air temperature, but the thermal comfort of occupants could still be ensured under such condition. Considering both the thermal comfort and the energyconservation, a ceiling temperature of 18°C (underside surface temperature of the ceiling) and an envelope temperature between 26°C and 32°C were proved appropriate for the summer. Similarly, based on the simulation results, a ceiling temperature of 26°C, and an envelope temperature between 8°C and 11°C were found appropriate for the winter. The results indicated that for the porous ceiling radiant air-conditioning system, ceiling temperature should be controlled to increase the ratio of radiant heat transfer in the summer, and the envelope temperature should be lowered to improve the energy-conservation of the system. In the winter, the heat transfer by radiation of the porous ceiling would account for a larger ratio, therefore the system showed good heating capacity and energyconservation performance in winter.publishedVersio
The microRNA ame-miR-279a regulates sucrose responsiveness of forager honey bees (Apis mellifera)
Increasing evidence demonstrates that microRNAs (miRNA) play an important role in the regulation of animal behaviours. Honey bees (Apis mellifera) are eusocial insects, with honey bee workers displaying age-dependent behavioural maturation. Many different miRNAs have been implicated in the change of behaviours in honey bees and ame-miR-279a was previously shown to be more highly expressed in nurse bee heads than in those of foragers. However, it was not clear whether this difference in expression was associated with age or task performance. Here we show that ame-miR-279a shows significantly higher expression in the brains of nurse bees relative to forager bees regardless of their ages, and that ame-miR-279a is primarily localized in the Kenyon cells of the mushroom body in both foragers and nurses. Overexpression of ame-miR-279a attenuates the sucrose responsiveness of foragers, while its absence enhances their sucrose responsiveness. Lastly, we determined that ame-miR-279a directly target the mRNA of Mblk-1. These findings suggest that ame-miR-279a plays important roles in regulating honey bee division of labour.This work was supported by grants of National Natural Science Foundation of China (31302039), Education Department Research Project of Anhui Province (2013SQRL018ZD), and the Open Fund of Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding (AKLGRCB2017007)
Dopamine and Serotonin Modulate Free Amino Acids Production and Na+/K+ Pump Activity in Chinese Mitten Crab Eriocheir sinensis Under Acute Salinity Stress
The Chinese mitten crab Eriocheir sinensis lives in saline or fresh water during different life stages and exhibits a complex life history, making it an ideal model to study the salinity adaptation of euryhaline animals. In this study, RNA-seq techniques, and determinations of free amino acids (FAAs), monoamine neurotransmitters, and Na+/K+ pump activity, were employed to understand the osmoregulatory mechanism in Chinese mitten crab. A total of 15,138 differentially expressed genes were obtained from 12 transcriptome libraries. GO enrichment analysis revealed that the mRNA expression profiles were completely remodeled from 12 to 24 h after salinity stress. The neuroendocrine system was activated under stimulation, and the monoamine neurotransmitters including dopamine (DA) and serotonin (5-HT) were released to modulate osmoregulation. Furthermore, the Na+/K+ pump in crab hemocytes was significantly inhibited post salinity stress, resulting in increased intracellular ion concentrations and osmotic pressure to sustain the osmotic balance. Moreover, six key FAAs, including alanine (Ala), proline (Pro), glycine (Gly), glutamate (Glu), arginine (Arg), and aspartate (Asp), were overexpressed to modulate the extracellular osmotic balance during salinity adaptation. Interestingly, the immune genes were not enriched in the GO analysis, implying that the immune system might not contribute fundamentally to the tolerance upon fluctuating ambient salinity in the Chinese mitten crab. These results collectively demonstrated that the Chinese mitten crab had evolved an efficient regulation mechanism by modulating the FAAs production and Na+/K+ pump activity to sustain the osmotic balance independent of the immune system, in which the neuroendocrine modulation, especially generated by the monoamine neurotransmitter, played an indispensable role
Fusion of hyperspectral imaging (HSI) and RGB for identification of soybean kernel damages using ShuffleNet with convolutional optimization and cross stage partial architecture
Identification of soybean kernel damages is significant to prevent further disoperation. Hyperspectral imaging (HSI) has shown great potential in cereal kernel identification, but its low spatial resolution leads to external feature infidelity and limits the analysis accuracy. In this study, the fusion of HSI and RGB images and improved ShuffleNet were combined to develop an identification method for soybean kernel damages. First, the HSI-RGB fusion network (HRFN) was designed based on super-resolution and spectral modification modules to process the registered HSI and RGB image pairs and generate super-resolution HSI (SR-HSI) images. ShuffleNet improved with convolution optimization and cross-stage partial architecture (ShuffleNet_COCSP) was used to build classification models with the optimal image set of effective wavelengths (OISEW) of SR-HSI images obtained by support vector machine and ShuffleNet. High-quality fusion of HSI and RGB with the obvious spatial promotion and satisfactory spectral conservation was gained by HRFN. ShuffleNet_COCSP and OISEW obtained the optimal recognition performance of ACCp=98.36%, Params=0.805 M, and FLOPs=0.097 G, outperforming other classification methods and other types of images. Overall, the proposed method provides an accurate and reliable identification of soybean kernel damages and would be extended to analysis of other quality indicators of various crop kernels
Mesoporous bioactive glass surface modified poly(lactic-co-glycolic acid) electrospun fibrous scaffold for bone regeneration
Chronic Myeloid Leukemia Patients Sensitive and Resistant to Imatinib Treatment Show Different Metabolic Responses
The BCR-ABL tyrosine kinase inhibitor imatinib is highly effective for chronic myeloid leukemia (CML). However, some patients gradually develop resistance to imatinib, resulting in therapeutic failure. Metabonomic and genomic profiling of patients' responses to drug interventions can provide novel information about the in vivo metabolism of low-molecular-weight compounds and extend our insight into the mechanism of drug resistance. Based on a multi-platform of high-throughput metabonomics, SNP array analysis, karyotype and mutation, the metabolic phenotypes and genomic polymorphisms of CML patients and their diverse responses to imatinib were characterized. The untreated CML patients (UCML) showed different metabolic patterns from those of healthy controls, and the discriminatory metabolites suggested the perturbed metabolism of the urea cycle, tricarboxylic acid cycle, lipid metabolism, and amino acid turnover in UCML. After imatinib treatment, patients sensitive to imatinib (SCML) and patients resistant to imatinib (RCML) had similar metabolic phenotypes to those of healthy controls and UCML, respectively. SCML showed a significant metabolic response to imatinib, with marked restoration of the perturbed metabolism. Most of the metabolites characterizing CML were adjusted to normal levels, including the intermediates of the urea cycle and tricarboxylic acid cycle (TCA). In contrast, neither cytogenetic nor metabonomic analysis indicated any positive response to imatinib in RCML. We report for the first time the associated genetic and metabonomic responses of CML patients to imatinib and show that the perturbed in vivo metabolism of UCML is independent of imatinib treatment in resistant patients. Thus, metabonomics can potentially characterize patients' sensitivity or resistance to drug intervention
Heavy Metals in Soil and Crops of an Intensively Farmed Area: A Case Study in Yucheng City, Shandong Province, China
Yucheng City is located in northwestern Shandong Province, China, and is situated on the Huang-Huai-Hai Plain, the largest alluvial plain in China. In this study, 86 surface soil samples were collected in Yucheng City and analyzed for cation exchange capacity (CEC), soil organic matter (SOM), pH, available phosphorus (avail. P), phosphorus (P), aluminum (Al), and iron (Fe). These soils were also analyzed for ‘total’ chromium (Cr), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), mercury (Hg), cadmium (Cd), and lead (Pb), together with 92 wheat samples and 37 corn samples. There was no obvious heavy metal contamination in the soil and irrigation water. But the long-term accumulation of heavy metals in soil has lead to an increase of Ni, As, Hg and Pb concentrations in some of wheat and corn samples and Cd in wheat samples. Because of the numerous sources of soil heavy metals and the lower level of heavy metal in irrigation water, there is no significant relation between soil heavy metal concentrations and irrigation water concentrations. Cr, Ni were mainly from the indigenous clay minerals according to multivariate analysis. Little contribution to soil heavy metal contents from agricultural fertilizer use was found and the local anomalies of As, Cd, Hg, Pb in wheat and corn grain are attributed to the interactive effects of irrigation and fertilizer used. Aerial Hg, however may also be the source of Hg for soil, wheat and corn
Urinary Arsenic Metabolites of Subjects Exposed to Elevated Arsenic Present in Coal in Shaanxi Province, China
In contrast to arsenic (As) poisoning caused by naturally occurring inorganic arsenic-contaminated water consumption, coal arsenic poisoning (CAP) induced by elevated arsenic exposure from coal combustion has rarely been reported. In this study, the concentrations and distributions of urinary arsenic metabolites in 57 volunteers (36 subjects with skin lesions and 21 subjects without skin lesions), who had been exposed to elevated levels of arsenic present in coal in Changshapu village in the south of Shaanxi Province (China), were reported. The urinary arsenic species, including inorganic arsenic (iAs) [arsenite (iAsIII) and arsenate (iAsV)], monomethylarsonic acid (MMAV) and dimethylarsinic acid (DMAV), were determined by high-performance liquid chromatography (HPLC) combined with inductively coupled plasma mass spectroscopy (ICP-MS). The relative distributions of arsenic species, the primary methylation index (PMI = MMAV/iAs) and the secondary methylation index (SMI = DMAV/MMAV) were calculated to assess the metabolism of arsenic. Subjects with skin lesions had a higher concentration of urinary arsenic and a lower arsenic methylation capability than subjects without skin lesions. Women had a significantly higher methylation capability of arsenic than men, as defined by a higher percent DMAV and SMI in urine among women, which was the one possible interpretation of women with a higher concentration of urinary arsenic but lower susceptibility to skin lesions. The findings suggested that not only the dose of arsenic exposure but also the arsenic methylation capability have an impact on the individual susceptibility to skin lesions induced by coal arsenic exposure
The Asian arowana (<i>Scleropages formosus</i>) genome provides new insights into the evolution of an early lineage of teleosts
The Asian arowana (Scleropages formosus), one of the world’s most expensive cultivated ornamental fishes, is an endangered species. It represents an ancient lineage of teleosts: the Osteoglossomorpha. Here, we provide a high-quality chromosome-level reference genome of a female golden-variety arowana using a combination of deep shotgun sequencing and high-resolution linkage mapping. In addition, we have also generated two draft genome assemblies for the red and green varieties. Phylogenomic analysis supports a sister group relationship between Osteoglossomorpha (bonytongues) and Elopomorpha (eels and relatives), with the two clades together forming a sister group of Clupeocephala which includes all the remaining teleosts. The arowana genome retains the full complement of eight Hox clusters unlike the African butterfly fish (Pantodon buchholzi), another bonytongue fish, which possess only five Hox clusters. Differential gene expression among three varieties provides insights into the genetic basis of colour variation. A potential heterogametic sex chromosome is identified in the female arowana karyotype, suggesting that the sex is determined by a ZW/ZZ sex chromosomal system. The high-quality reference genome of the golden arowana and the draft assemblies of the red and green varieties are valuable resources for understanding the biology, adaptation and behaviour of Asian arowanas
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