Association of the LEP gene with immune infiltration as a diagnostic biomarker in preeclampsia

Objective: Preeclampsia (PE) is a serious condition in pregnant women and hence an important topic in obstetrics. The current research aimed to recognize the potential and significant immune-related diagnostic biomarkers for PE.Methods: From the Gene Expression Omnibus (GEO) data sets, three public gene expression profiles (GSE24129, GSE54618, and GSE60438) from the placental samples of PE and normotensive pregnancy were downloaded. Differentially expressed genes (DEGs) were selected and determined among 73 PE and 85 normotensive control pregnancy samples. The DEGs were used for Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Disease Ontology (DO) enrichment analysis, and Gene Set Enrichment Analysis (GSEA). The candidate biomarkers were identified by the least absolute shrinkage and selection operator (LASSO) and support vector machine recursive feature elimination (SVM-RFE) analysis. The receiver operating characteristic curve (ROC) was applied to evaluate diagnostic ability. For further confirmation, the expression levels and diagnostic value of biomarkers in PE were verified in the GSE75010 data set (80 PE and 77 controls) and validated by qRT-RCR, Western blot, and immunohistochemistry (IHC). The CIBERSORT algorithm was used to calculate the compositional patterns of 22 types of immune cells in PE.Results: In total, 15 DEGs were recognized. The GO and KEGG analyses revealed that the DEGs were enriched in the steroid metabolic process, receptor ligand activity, GnRH secretion, and neuroactive ligand−receptor interaction. The recognized DEGs were primarily implicated in cell-type benign neoplasm, kidney failure, infertility, and PE. Gene sets related to hormone activity, glycosylation, multicellular organism process, and response to BMP were activated in PE. The LEP gene was distinguished as a diagnostic biomarker of PE (AUC = 0.712) and further certified in the GSE75010 data set (AUC = 0.850). The high expression of LEP was associated with PE in clinical samples. In addition, the analysis of the immune microenvironment showed that gamma delta T cells, memory B cells, M0 macrophages, and regulatory T cells were positively correlated with LEP expression (P < 0.05).Conclusion:LEP expression can be considered to be a diagnostic biomarker of PE and can offer a novel perspective for future studies regarding the occurrence and molecular mechanisms of PE

Research Methods of Main Parameter Sensitivity Differences in China’s Dynamic Oil and Gas Reserve Estimation under SEC Standards

International oil and gas companies listed in New York must publish the information of oil and gas reserves under the SEC (United States Securities and Exchange Commission) standards every year. For greatly improving the SEC reserve, the SEC reserve value and the SEC reserve substitution rate, in this article not only the SEC reserve equations have been determined but also the SEC reserve value models have been established. The SEC reserve value models have been verified as correct. Based on these models, the multivariate function calculus method, the multivariate function limit method and the function recurrence method have been adopted to research parameter sensitivity differences rules, parameter adjustment directions, parameter adjustment degrees and SEC reserve parameter linkage adjustment rules. The research is significant, because there are great differences between SEC standards and China’s in reserve management mode, reserve estimation method system and financial management system. It is just these differences that cause the frequent adjustment of SEC reserve parameters during the process of SEC reserve submissions each year. As a result, this article reaches some conclusions. Above all, the article has clarified the parameter quantitative conditions that lead to the sensitivity between the SEC reserve and the initial production to begin stronger and weaker than the sensitivity between the SEC reserve and the price in production exponential, hyperbolic and harmonic decline types. Furthermore, the article has clarified the parameter quantitative conditions that lead to the sensitivity between the SEC reserve value and the initial production to begin stronger and weaker than the sensitivity between the SEC reserve value and the price in common production exponential decline types. Moreover, the article has clarified reserve parameter linkage adjustment rules and found the most significant parameter whose least adjustment will cause the largest reserve increase. In addition, the function calculus method adopted to disclose reserve parameter sensitivity rules will expand the parameter sensitivity analysis method that took the previous statistical mapping method as the main analysis method

Research Methods of Main Parameter Sensitivity Differences in China’s Dynamic Oil and Gas Reserve Estimation under SEC Standards

International oil and gas companies listed in New York must publish the information of oil and gas reserves under the SEC (United States Securities and Exchange Commission) standards every year. For greatly improving the SEC reserve, the SEC reserve value and the SEC reserve substitution rate, in this article not only the SEC reserve equations have been determined but also the SEC reserve value models have been established. The SEC reserve value models have been verified as correct. Based on these models, the multivariate function calculus method, the multivariate function limit method and the function recurrence method have been adopted to research parameter sensitivity differences rules, parameter adjustment directions, parameter adjustment degrees and SEC reserve parameter linkage adjustment rules. The research is significant, because there are great differences between SEC standards and China’s in reserve management mode, reserve estimation method system and financial management system. It is just these differences that cause the frequent adjustment of SEC reserve parameters during the process of SEC reserve submissions each year. As a result, this article reaches some conclusions. Above all, the article has clarified the parameter quantitative conditions that lead to the sensitivity between the SEC reserve and the initial production to begin stronger and weaker than the sensitivity between the SEC reserve and the price in production exponential, hyperbolic and harmonic decline types. Furthermore, the article has clarified the parameter quantitative conditions that lead to the sensitivity between the SEC reserve value and the initial production to begin stronger and weaker than the sensitivity between the SEC reserve value and the price in common production exponential decline types. Moreover, the article has clarified reserve parameter linkage adjustment rules and found the most significant parameter whose least adjustment will cause the largest reserve increase. In addition, the function calculus method adopted to disclose reserve parameter sensitivity rules will expand the parameter sensitivity analysis method that took the previous statistical mapping method as the main analysis method

Toxic effect of three imidazole ionic liquids on two terrestrial plants

To determine the toxic effect of three imidazole ionic liquids (IILs) in terrestrial monocotyledonous and dicotyledonous plants, three IILs (1-butyl-3-methylimidazole tetrafluoroborate, 1-butyl-3-methylimidazole hexafluorophosphate, and butyl-3-methylimidazolium bi-[(trifluoromethyl)sulfonyl]imide) were investigated using rice and capsicum as target toxicity models. In hydroponic experiments, increasing the concentration of the IILs led to a decrease in the seed germination rate, a decrease in the reduced stem and root lengths, and an increase in the inhibition rate of the stem and root lengths; in addition, as the concentration increased, the reducing sugar content of rice and capsicum seedling leaves and roots first increased and then decreased, while permeability of the cell membranes of the stems and roots of the two plants also gradually increased. In terms of the effects on these indices in rice, the ranking of these three IIL anions was [TF2N]- > [PF6]- > [BF4]-; in terms of the effects on capsicum, the sequence was [BF4]- > [TF2N]- > [PF6]-. These findings provide a theoretical reference for the next step in the synthesis and the use of green ionic liquids

Validation and psychometric testing of the Chinese version of the prenatal body image questionnaire

Abstract Background The body image during pregnancy potentially affects both short- and long-term maternal and child health outcomes, including pregnancy mood, postpartum weight recovery, and the quality of mother–child interactions. However, research on the impact of body changes during pregnancy in the Chinese population is scarce. A comprehensive, practical, and reliable tool for assessing pregnant women is needed to detect, intervene in, and implement the reduction of physical dissatisfaction risk. This study translated the Prenatal Body Image Questionnaire (PBIQ) into the Chinese version (PBIQ-C) to assess the body image of pregnant women and evaluated its reliability and validity. Methods An improved Brislin translation model was used for the translation. A panel of experts determined the content validity. A convenience sample of 429 pregnant women was chosen from three third-class hospitals in different regions of Fujian Province, China. Factor analysis, Pearson’s correlation, retest reliability, and Cronbach’s alpha were employed to evaluate structural validity and reliability. Results The final PBIQ-C had five dimensions with 21 items. Exploratory factor analysis obtained a five-factor solution, which accounted for a total of 60.34%. Confirmatory factor analysis showed that the model fit of the five-factor model also reached a satisfactory model fit after modifying: The Comparative Fit Index was 0.93, and the Tucker-Lewis Index was 0.92; the Root Mean Square Error of Approximation was 0.079. The content validity index of the scale ranged from 0.63 ~ 1.00. The Cronbach’s alpha coefficient was 0.95 for the total scale, and the test–retest reliability was 0.80. Conclusions The findings indicated that the PBIQ-C is a valid and reliable instrument for assessing women’s body image during pregnancy, which helps in the early identification of body dissatisfaction during pregnancy and enables the early prevention of postpartum depression

Volumetric Compression Induces Intracellular Crowding to Control Intestinal Organoid Growth via Wnt/β-Catenin Signaling

© 2020 Elsevier Inc. Enormous amounts of essential intracellular events are crowdedly packed inside picoliter-sized cellular space. However, the significance of the physical properties of cells remains underappreciated because of a lack of evidence of how they affect cellular functionalities. Here, we show that volumetric compression regulates the growth of intestinal organoids by modifying intracellular crowding and elevating Wnt/β-catenin signaling. Intracellular crowding varies upon stimulation by different types of extracellular physical/mechanical cues and leads to significant enhancement of Wnt/β-catenin signaling by stabilizing the LRP6 signalosome. By enhancing intracellular crowding using osmotic and mechanical compression, we show that expansion of intestinal organoids was facilitated through elevated Wnt/β-catenin signaling and greater intestinal stem cell (ISC) self-renewal. Our results provide an entry point for understanding how intracellular crowdedness functions as a physical regulator linking extracellular physical cues with intracellular signaling and potentially facilitate the design of engineering approaches for expansion of stem cells and organoids

Plasma Metabolomics Reveals β-Glucan Improves Muscle Strength and Exercise Capacity in Athletes

The present study aimed to assess the changes in muscle strength and plasma metabolites in athletes with β-glucan supplementation. A total of 29 athletes who met the inclusion criteria were recruited for this study (ChiCTR2200058091) and were randomly divided into a placebo group (n = 14) and β-glucan group (n = 15). During the trial, the experimental group received β-glucan supplementation (2 g/d β-glucan) for 4 weeks and the control group received an equal dose of placebo supplementation (0 g/d β-glucan), with both groups maintaining their regular diet and exercise habits during the trial. The athletes’ exercise performance, muscle strength, and plasma metabolome changes were analyzed after 4 weeks of β-glucan supplementation. The results showed a significant increase in mean grip strength (kg), right hand grip strength (kg), left triceps strength (kg), and upper limb muscle mass (kg) in the experimental group after the 4-week intervention compared to the preintervention period (p < 0.05). A comparison of the difference between the two groups after the intervention showed that there were significant differences between the control group and the experimental group in mean grip strength (kg) and right-hand grip strength (kg) (p < 0.05). Athletes in the experimental group showed significant improvements in 1 min double rocking jump (pcs), VO2max (ml/kg-min) (p < 0.05). The β-glucan intake increased the creatine-related pathway metabolites in plasma. Overall, these results suggest that 4 weeks of β-glucan supplementation can improve muscle strength in athletes, with the potential to increase aerobic endurance and enhance immune function, possibly by affecting creatine-related pathways