4,203 research outputs found
Design and Analysis of the High-Order Mode Dispersion Compensating Fiber
We discussed how to design the typical trip-clad high-order mode fiber (HOMF) profiles to achieve the required dispersion properties based on LP(02) mode, to compensate all modern transmission fibers, without sacrificing other important properties, such as effective area. Finally, HOMF compensating 100km eLEAF (R) fiber has been designed. Its dispersion at 1550nm is -1217ps/nm/km, and the relative dispersion slope (RDS) is 0.02nm(-1). Only similar to 345m of HOMF is needed to achieve full dispersion and dispersion slope compensation of the span, while maintaining effective area above 52 mu m(2) over the entire C-band
Mutational analysis of the Ve1 immune receptor that mediates Verticillium resistance in tomato
Pathogenic Verticillium species are economically important plant pathogens that cause vascular wilt diseases in hundreds of plant species. The Ve1 gene of tomato confers resistance against race 1 strains of Verticillium dahliae and V. albo-atrum. Ve1 encodes an extracellular leucine-rich repeat (eLRR) receptor-like protein (RLP) that serves as a cell surface receptor for recognition of the recently identified secreted Verticillium effector Ave1. To investigate recognition of Ave1 by Ve1, alanine scanning was performed on the solvent exposed ß-strand/ß-turn residues across the eLRR domain of Ve1. In addition, alanine scanning was also employed to functionally characterize motifs that putatively mediate protein-protein interactions and endocytosis in the transmembrane domain and the cytoplasmic tail of the Ve1 protein. Functionality of the mutant proteins was assessed by screening for the occurrence of a hypersensitive response upon co-expression with Ave1 upon Agrobacterium tumefaciens-mediated transient expression (agroinfiltration). In order to confirm the agroinfiltration results, constructs encoding Ve1 mutants were transformed into Arabidopsis and the transgenes were challenged with race 1 Verticillium. Our analyses identified several regions of the Ve1 protein that are required for functionality
Hot Jupiters from Secular Planet--Planet Interactions
About 25 per cent of `hot Jupiters' (extrasolar Jovian-mass planets with
close-in orbits) are actually orbiting counter to the spin direction of the
star. Perturbations from a distant binary star companion can produce high
inclinations, but cannot explain orbits that are retrograde with respect to the
total angular momentum of the system. Such orbits in a stellar context can be
produced through secular (that is, long term) perturbations in hierarchical
triple-star systems. Here we report a similar analysis of planetary bodies,
including both octupole-order effects and tidal friction, and find that we can
produce hot Jupiters in orbits that are retrograde with respect to the total
angular momentum. With distant stellar mass perturbers, such an outcome is not
possible. With planetary perturbers, the inner orbit's angular momentum
component parallel to the total angular momentum need not be constant. In fact,
as we show here, it can even change sign, leading to a retrograde orbit. A
brief excursion to very high eccentricity during the chaotic evolution of the
inner orbit allows planet-star tidal interactions to rapidly circularize that
orbit, decoupling the planets and forming a retrograde hot Jupiter.Comment: accepted for publication by Nature, 3 figures (version after proof -
some typos corrected
The Role of Biomethylation in Toxicity and Carcinogenicity of Arsenic: A Research Update
Recent research of the metabolism and biological effects of arsenic has profoundly changed our understanding of the role of metabolism in modulation of toxicity and carcinogenicity of this metalloid. Historically, the enzymatic conversion of inorganic arsenic to mono- and dimethylated species has been considered a major mechanism for detoxification of inorganic arsenic. However, compelling experimental evidence obtained from several laboratories suggests that biomethylation, particularly the production of methylated metabolites that contain trivalent arsenic, is a process that activates arsenic as a toxin and a carcinogen. This article summarizes this evidence and provides new data on a) the toxicity of methylated trivalent arsenicals in mammalian cells, b) the effects of methylated trivalent arsenicals on gene transcription, and c) the mechanisms involved in arsenic methylation in animal and human tissues
Invariant Distribution of Promoter Activities in Escherichia coli
Cells need to allocate their limited resources to express a wide range of genes. To understand how Escherichia coli partitions its transcriptional resources between its different promoters, we employ a robotic assay using a comprehensive reporter strain library for E. coli to measure promoter activity on a genomic scale at high-temporal resolution and accuracy. This allows continuous tracking of promoter activity as cells change their growth rate from exponential to stationary phase in different media. We find a heavy-tailed distribution of promoter activities, with promoter activities spanning several orders of magnitude. While the shape of the distribution is almost completely independent of the growth conditions, the identity of the promoters expressed at different levels does depend on them. Translation machinery genes, however, keep the same relative expression levels in the distribution across conditions, and their fractional promoter activity tracks growth rate tightly. We present a simple optimization model for resource allocation which suggests that the observed invariant distributions might maximize growth rate. These invariant features of the distribution of promoter activities may suggest design constraints that shape the allocation of transcriptional resources
Identification of hip fracture patients from radiographs using Fourier analysis of the trabecular structure: a cross-sectional study
Peer reviewedPublisher PD
General Argyres-Douglas Theory
We construct a large class of Argyres-Douglas type theories by compactifying
six dimensional (2,0) A_N theory on a Riemann surface with irregular
singularities. We give a complete classification for the choices of Riemann
surface and the singularities. The Seiberg-Witten curve and scaling dimensions
of the operator spectrum are worked out. Three dimensional mirror theory and
the central charges a and c are also calculated for some subsets, etc. Our
results greatly enlarge the landscape of N=2 superconformal field theory and in
fact also include previous theories constructed using regular singularity on
the sphere.Comment: 55 pages, 20 figures, minor revision and typos correcte
Specific β-Tubulin Isotypes Can Functionally Enhance or Diminish Epothilone B Sensitivity in Non-Small Cell Lung Cancer Cells
Epothilones are a new class of microtubule stabilizing agents with promising preclinical and clinical activity. Their cellular target is β-tubulin and factors influencing intrinsic sensitivity to epothilones are not well understood. In this study, the functional significance of specific β-tubulin isotypes in intrinsic sensitivity to epothilone B was investigated using siRNA gene knockdown against βII-, βIII- or βIVb-tubulins in two independent non-small cell lung cancer (NSCLC) cell lines, NCI-H460 and Calu-6. Drug-treated clonogenic assays showed that sensitivity to epothilone B was not altered following knockdown of βII-tubulin in both NSCLC cell lines. In contrast, knockdown of βIII-tubulin significantly increased sensitivity to epothilone B. Interestingly, βIVb-tubulin knockdowns were significantly less sensitive to epothilone B, compared to mock- and control siRNA cells. Cell cycle analysis of βIII-tubulin knockdown cells showed a higher percentage of cell death with epothilone B concentrations as low as 0.5 nM. In contrast, βIVb-tubulin knockdown cells displayed a decrease in epothilone B-induced G2-M cell cycle accumulation compared to control siRNA cells. Importantly, βIII-tubulin knockdowns displayed a significant dose-dependent increase in the percentage of apoptotic cells upon treatment with epothilone B, as detected using caspase 3/7 activity and Annexin-V staining. Higher concentrations of epothilone B were required to induce apoptosis in the βIVb-tubulin knockdowns compared to control siRNA, highlighting a potential mechanism underlying decreased sensitivity to this agent. This study demonstrates that specific β-tubulin isotypes can influence sensitivity to epothilone B and may influence differential sensitivity to this promising new agent
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