20 research outputs found
SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in Crassostrea gigas
The self-renewal and differentiation of germ cells are essential for gametogenesis and reproduction. In mammals, the transcription factors SOHLH1 and SOHLH2, two members of the bHLH family, are specifically expressed in the gonads, and play an important role in spermatocyte and oocyte differentiation. In our previous study, we performed a phylogenetic analysis of the Lophotrochozoa bHLH genes, and two Sohlh were identified in the Pacific oyster Crassostrea gigas. Based on the genomes of other species that have complete genomic information, we further analyzed the phylogenetics of the Sohlh in this study. The results indicate that the Sohlh are ancient genes that were lost in many species during evolution, including in some invertebrates, and lower vertebrates. The phylogenetic tree shows that Sohlh1 and Sohlh2 are located in different scaffolds and that they have low similarity, suggesting early separation in invertebrates. We used RNA-seq and RT-PCR to examine the mRNA expression of the Sohlh in C. gigas (termed Cg-Sohlh), we found that Cg-Sohlh1, and Cg-Sohlh2 are specifically expressed in the gonads. During gonadal development, the mRNA expression levels of both genes increased from the proliferative stage and reached the highest level at the growth stage (P < 0.05). Then, the expression level decreased until the resting stage. In addition, immunohistochemistry was used to determine that the Cg-SOHLH1 protein was specifically expressed in the spermatogonia and spermatocytes. Cg-Sohlh2 mRNA was expressed in both the male and female gonads, while Cg-Sohlh1 mRNA was highly expressed in the female gonads at all developmental stages except for the resting stage. These data indicate that Cg-SOHLH might be gonad-specific regulatory factors, similar to mammalian SOHLH, and that Cg-SOHLH1 might be involved in spermatogonial differentiation. This study lays the foundation to further determine the functional role of SOHLH in mollusk gametogenesis and provides a foundation to better understand the regulatory mechanism of gametogenesis in invertebrates
Application and development of synchronous fluorescence spectrometry
Synchronous fluorescence spectrometry is useful in simultaneous determination of multi-component fluorescent substances. Its application and development in recent years are reviewed. Synchronous fluorescence approaches, including constant-wavelength synchronous fluorescence, constant-energy synchronous fluorescence, variable-angle synchronous fluorescence, isopotential matrix synchronous fluorescence, and the combination with the derivative technique, stoichiometry and cryogenic technique are discussed
Metagenomic insights into the abundance and composition of resistance genes in aquatic environments:Influence of stratification and geography
A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)-1.34x10(0) and 1.22x10(-3) -1.98x10(-1) copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multimetal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater
a multimodal 3d storytelling system for chinese children
storytelling by a child, as an educational activity, influences significantly the childs linguistic ability, thought process, imagination, and creativity. There presently exist many software-based storytelling applications. However, most are
Preparation of Silver Nanoparticles Loaded Photoresponsive Composite Microgels and Their Light-Controllable Catalytic Activity
Preparation of Silver Nanoparticles Loaded Photoresponsive Composite Microgels and Their Light-Controllable Catalytic Activity
A new type of smart composite microgels,
which are able to control the catalytic activity of their loaded silver
nanoparticles by light, was designed and fabricated based on the idea
of function transfer between their constituent components. First,
the surfaces of monodisperse gold nanorods (AuNRs) with strong photothermal
effect were coated with poly(<i>N</i>-isopropylacrylamide)
(PNIPAM) hydrogel by seed precipitation polymerization to prepare
the two-component composite microgels with core–shell structure
(AuNR@PNIPAM microgels). Then, Ag<sup>+</sup> ions coordinated into
the shell of AuNR@PNIPAM microgels were <i>in situ</i> reduced
by sodium borohydride to produce silver nanoparticles (AgNPs) loaded
three-component composite microgels (AuNR@(AgNPs/PNIPAM) microgels).
The characterization results obtained by transmission electron microscopy
show that the gold nanorod is located at the center of the three-component
composite microgels and AgNPs with the average particle diameters
of 6–10 nm are evenly distributed within its shell. The hydrodynamic
diameters of the composite microgels, measured by dynamic light scattering
before or after exposure of their aqueous dispersion to near-infrared
(NIR) laser of 808 nm wavelength, indicate that they have photoresponsive
property. The AgNPs and AuNR inside AuNR@(AgNPs/PNIPAM) microgels
hold their respective localized surface plasmon resonance (LSPR) optical
property, and the longitudinal LSPR wavelength of the latter is blue-shifted
with increasing content of the former. Moreover, the LSPR efficiency
of the AgNPs and the longitudinal LSPR wavelength of the AuNR are
capable of being changed in response to the NIR illumination, and
the stimulus-responsive behavior is reversible. AuNR@(AgNPs/PNIPAM)
microgels are able to be used as the smart microreactor for reducing
4-nitrophenol by NaBH<sub>4</sub>, and the reaction rate can be modulated
by power density of the NIR light, demonstrating that the three-component
composite microgels have light-controllable catalytic activity
Medical Visual Question Answering: A Survey
Medical Visual Question Answering~(VQA) is a combination of medical
artificial intelligence and popular VQA challenges. Given a medical image and a
clinically relevant question in natural language, the medical VQA system is
expected to predict a plausible and convincing answer. Although the
general-domain VQA has been extensively studied, the medical VQA still needs
specific investigation and exploration due to its task features. In the first
part of this survey, we collect and discuss the publicly available medical VQA
datasets up to date about the data source, data quantity, and task feature. In
the second part, we review the approaches used in medical VQA tasks. We
summarize and discuss their techniques, innovation, and potential improvement.
In the last part, we analyze some medical-specific challenges for the field and
discuss future research directions. Our goal is to provide comprehensive
information for researchers interested in medical artificial intelligence
Macromolecules with Different Charges, Lengths, and Coordination Groups for the Coprecipitation Synthesis of Magnetic Iron Oxide Nanoparticles as <i>T</i><sub>1</sub> MRI Contrast Agents
Considerable efforts have been focused on the exploitation of macromolecule ligands for synthesis of magnetic Fe3O4 nanoparticles as T1 magnetic resonance imaging (MRI) contrast agents, but studies that concern macromolecule ligands with different charges and coordination groups are still limited. Herein, we used poly(acrylic acid) (PAA), poly(allylamine hydrochloride) (PAH), and polyvinyl alcohol (PVA), which possess negative, positive and neutral charges with carboxylic acid, amino and hydroxyl groups respectively, as templates and stabilizers to fabricate Fe3O4 nanoparticles through coprecipitation reaction. The obtained Fe3O4-PAA, Fe3O4-PAH, and Fe3O4-PVA nanoparticles showed T1 contrast performance with r1 relaxivities of 23.4, 60.3, and 30.6 mM s−1 at 0.5 T (25 °C), and a r2/r1 ratio of 2.62, 3.82, and 7.26, respectively. The cell viability assay revealed that Fe3O4-PAA and Fe3O4-PVA exhibited good biocompatibility, while Fe3O4-PAH displayed high cytotoxicity. In vivo T1-weighted (1 T) mice showed that both Fe3O4-PAA and Fe3O4-PVA were able to display remarkably brighten the contrast enhancement for the mice tumor and kidney sites, but Fe3O4-PAA had better contrast performance. This work highlights that the macromolecule ligands play an important role in the biocompatibility and T1 contrast performance of magnetic Fe3O4 nanoparticles
Ancestral niche separation and evolutionary rate differentiation between sister marine flavobacteria lineages
Marine flavobacteria are specialists for polysaccharide degradation. They dominate in habitats enriched with polysaccharides, but are also prevalent in pelagic environments where polysaccharides are less available. These niches are likely occupied by distinct lineages, but evolutionary processes underlying their niche differentiation remain elusive. Here, genomic analyses and physiological assays indicate that the sister flavobacteria lineages Leeuwenhoekiella and Nonlabens likely explore polysaccharide-rich macroalgae and polysaccharide-poor pelagic niches respectively. Phylogenomic analyses inferred that the niche separation likely occurred anciently and coincided with increased sequence evolutionary rate in Nonlabens compared with Leeuwenhoekiella. Further analyses ruled out the known mechanisms likely driving evolutionary rate acceleration, including reduced selection efficiency, decreased generation time and increased mutation rate. In particular, the mutation rates were determined using an unbiased experimental method, which measures the present-day populations and may not reflect ancestral populations. These data collectively lead to a new hypothesis that an ancestral and transient mutation rate increase resulted in evolutionary rate increase in Nonlabens. This hypothesis was supported by inferring that gains and losses of genes involved in SOS response, a mechanism known to drive transiently increased mutation rate, coincided with evolutionary rate acceleration. Our analyses highlight the evolutionary mechanisms underlying niche differentiation of flavobacteria lineages.</p