Genetic variability of indigenous cowpea genotypes as determined using inter-simple sequence repeats markers

Cowpea [Vigna unguiculata (L.) Walp.] is cultivated widely by small farmers in the semiarid region of Northeastern Brazil for subsistence purposes, especially to complement the family income. However, owing to the limited availability of water in this region, there is an urgent need for novel highly productive drought-tolerant cultivars. The aim of the present study was to establish the genetic variability of 14 cowpea populations (60 indigenous genotypes from 13 microregions of Rio Grande do Norte and 4 domesticated cultivars produced by Embrapa) using inter-simple sequence repeats (ISSR) markers. The set of 13 selected primers generated a total of 257 loci, 247 (96.11%) of which were polymorphic, with sizes ranging between 200 and 2000 bp. Genetic similarities between accessions were estimated from Jaccard coefficients and genetic relationships were determined from the dendrogram constructed using the unweighted pair group method with arithmetic average (UPGMA) technique. Bayesian statistics  coupled with the Markov chain Monte Carlo technique was applied to determine population structure, while the genetic variability was established by analysis of molecular variance. UPGMA analysis allowed the separation of the genotypes into three groups, but no relationship between the genetic and  geographical distances was observed. The fixation index was considered intermediary (FST = 0.0818), the average heterozygosity was low (HS = 0.39) and the coefficient of endogamy was high (f = 92.6%). The results show the presence of genetic diversity among the studied populations and revealed that such variability could be attributed mainly to intra-population variability (91.82%).Key words: Vigna unguiculata, genetic diversity, plant improvement, drought stress

Molecular landscape of esophageal cancer: implications for early detection and personalized therapy

Esophageal cancer (EC) is one of the most lethal cancers and a public health concern worldwide, owing to late diagnosis and lack of efficient treatment. Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC) are main histopathological subtypes of EC that show striking differences in geographical distribution, possibly due to differences in exposure to risk factors and lifestyles. ESCC and EAC are distinct diseases in terms of cell of origin, epidemiology, and molecular architecture of tumor cells. Past efforts aimed at translating potential molecular candidates into clinical practice proved to be challenging, underscoring the need for identifying novel candidates for early diagnosis and therapy of EC. Several major international efforts have brought about important advances in identifying molecular landscapes of ESCC and EAC toward understanding molecular mechanisms and critical molecular events driving the progression and pathological features of the disease. In our review, we summarize recent advances in the areas of genomics and epigenomics of ESCC and EAC, their mutational signatures and immunotherapy. We also discuss implications of recent advances in characterizing the genome and epigenome of EC for the discovery of diagnostic/prognostic biomarkers and development of new targets for personalized treatment and prevention

Does caffeine ingestion before a short-term sprint interval training promote body fat loss?

We investigated the effect of caffeine ingestion combined with a 2-wk sprint interval training (SIT) on training-induced reductions in body adiposity. Twenty physically-active men ingested either 5 mg/kg of cellulose as a placebo (PLA, n=10) or 5 mg/kg of caffeine (CAF, n=10) 60 min before each SIT session (13×30 s sprint/15 s of rest). Body mass and skinfold thickness were measured pre- and post-training. Energy expenditure was measured at rest, during exercise, and 45 min after exercise in the first SIT session. Body fat was similar between PLA and CAF groups at pre-training (P\u3e0.05). However, there was a significant decrease in body fat after training in the CAF group (−5.9±4.2%, P\u3c0.05) but not in PLA (1.5±8.0%, P\u3e0.05). There was no difference in energy expenditure at rest and during exercise between PLA and CAF groups (P\u3e0.05), but the post-exercise energy expenditure was 18.3±21.4% greater in the CAF than in the PLA group (P\u3c0.05). In conclusion, caffeine ingestion before SIT sessions induced a body fat loss that may be associated with higher post-exercise energy expenditure

Evaluation of the integration level of quality and environmental management systems in a tire manufacturer

Purpose: This article aims to evaluate the integration level of a quality management system (QMS) and an environmental management system (EMS) in a tire manufacturer and propose a guide to evaluate the integration of these systems in companies. Design/methodology/approach: The methodological strategies used in this research were literature review; and case study, with interviews to verify professionals' perception about benefits from integration. Data from interviews were analyzed through Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS). Findings: The results showed that the studied company has a simple level of integration, observing only some low intensity benefits. Thus, it was recommended that the company partially integrate its management systems (MSs) before evolving into something more complex. The literature and the findings of case study were used as basis for proposing a guide to evaluate MS integration. Originality/value: Lessons learned throughout the study and the suggested guide can support other companies to assess the integration level of their QMS and EMS. Thus, the findings presented here can be useful for researchers and managers

Critical analysis of engineering education focused on sustainability in supply chain management: an overview of Brazilian higher education institutions

© 2020, Emerald Publishing Limited. Purpose: This paper aims to critically analyze the engineering education focused on sustainability in supply chain management, in courses offered by Brazilian higher education institutions. Design/methodology/approach: Topics related to sustainable supply chain management were listed from the literature and used as a framework to gather professors’ opinions on how well these topics are covered in engineering courses offered in Brazil. Data analysis was performed via frequency analysis and comparative ordering using the Fuzzy technique for order preference by similarity to ideal solution technique. Findings: It was possible to evidence that most of the topics are superficially presented within other subjects and that there are few associated practical activities that enable greater learning. Comparatively, issues related to ISO standards (related to quality and environmental management systems) and compliance with environmental laws, regulations and standards were highlighted. Additionally, it was possible to verify that there is a need for further study on issues related to energy efficiency, worker training and corporate governance. Originality/value: No similar study was found in the literature. The findings presented in this paper can contribute to the improvement of engineering education in Brazil and other countries