CMOS optoelectronics: Implementation and application.

An investigation of three different photo sensitive devices (PSD) that can be fabricated by using Northern Telecom\u27s 1.2 and 3.0 micro CMOS technology is described in the thesis. A MOSFET has been optimized to enhance the photocurrent and use a parasitic photodiode formed between the source and substrate as a PSD. A conventional BJT structure with a small emitter area has been optimized to enhance the photocurrent and utilize the parasitic photodiode formed at the base-collector junction as a PSD. In addition, a field effect modified (FEM) vertical BJT with a collector-connected annular ring around a small emitter area has been used to create a more sensitive and faster responding parasitic photodiode at the base-collector junction that can be used as a PSD. Each of the three distinct structures has been fabricated in both 1.2 and 3.0 micro technology in a number of different geometries as part of a parameter optimization study. A number of experiments have been carried out on the test cells to measure photocurrent as a function of light intensity using incandescent and LASER light sources. The most sensitive PSD was formed using a 3.0 micro FEM BJT design. The device is being used to create a photo sensitive array that will act as input nodes for an artificial neural network that is being employed as an intelligent sensor for process control based on non-contact measurement. As the array will be used to image LASER generated patterns formed by object-oriented beam steering, high sensitivity is not necessary, whereas a structure that can be readily integrated into a regular array is most important. Source: Masters Abstracts International, Volume: 33-04, page: 1307. Adviser: W. C. Miller. Thesis (M.Sc.A.)--University of Windsor (Canada), 1994

The nanoscale phase distinguishing of PCL-PB-PCL blended in epoxy resin by tapping mode atomic force microscopy

In this work, we investigated the bulk phase distinguishing of the poly(ε-caprolactone)-polybutadiene-poly(ε-caprolactone) (PCL-PB-PCL) triblock copolymer blended in epoxy resin by tapping mode atomic force microscopy (TM-AFM). We found that at a set-point amplitude ratio (rsp) less than or equal to 0.85, a clear phase contrast could be obtained using a probe with a force constant of 40 N/m. When rsp was decreased to 0.1 or less, the measured size of the PB-rich domain relatively shrank; however, the height images of the PB-rich domain would take reverse (translating from the original light to dark) at rsp = 0.85. Force-probe measurements were carried out on the phase-separated regions by TM-AFM. According to the phase shift angle vs. rsp curve, it could be concluded that the different force exerting on the epoxy matrix or on the PB-rich domain might result in the height and phase image reversion. Furthermore, the indentation depth vs. rsp plot showed that with large tapping force (lower rsp), the indentation depth for the PB-rich domain was nearly identical for the epoxy resin matrix

Genotyping of TRIM5 locus in northern pig-tailed macaques (Macaca leonina), a primate species susceptible to Human Immunodeficiency Virus type 1 infection

<p>Abstract</p> <p>Background</p> <p>The pig-tailed macaques are the only Old World monkeys known to be susceptible to human immunodeficiency virus type 1 (HIV-1) infection. We have previously reported that the <it>TRIM5-Cyclophilin A </it>(<it>TRIMCyp</it>) fusion in pig-tailed macaques (<it>Macaca nemestrina</it>) is dysfunctional in restricting HIV-1, which may explain why pig-tailed macaques are susceptible to HIV-1 infection. Similar results have also been reported by other groups. However, according to the current primate taxonomy, the previously reported <it>M. nemestrina </it>are further classified into three species, which all belong to the <it>Macaca spp</it>. This calls for the need to look into the previous studies in more details.</p> <p>Results</p> <p>The local species Northern pig-tailed macaque (<it>M. leonina</it>) was analyzed for the correlation of <it>TRIM5 </it>structure and HIV-1 infection. Eleven <it>M. leonina </it>animals were analyzed, and all of them were found to possess <it>TRIM5-CypA </it>fusion at the <it>TRIM5 </it>locus. The transcripts encoding the dysfunctional <it>TRIM5-CypA </it>should result from the G-to-T mutation in the 3'-splicing site of intron 6. Polymorphism in the putative TRIMCyp recognition domain was observed. The peripheral blood mononuclear cells (PBMCs) of <it>M. leonina </it>were susceptible to HIV-1 infection. Consistent with the previous results, expression of the <it>M. leonina </it>TRIMCyp in HeLa-T4 cells rendered the cells resistant to HIV-2_RODbut not to SIVmac239 infection.</p> <p>Conclusion</p> <p>The susceptibility of <it>M. leonina </it>to HIV-1 infection is due to the dysfunctional <it>TRIM5-CypA </it>fusion in the <it>TRIM5 </it>locus. This finding should broaden our perspective in developing better HIV/AIDS non-human primate animal models.</p

HIV-1 can infect northern pig-tailed macaques (Macaca leonina) and form viral reservoirs in vivo

Viral reservoirs of HIV-1 are a major obstacle for curing AIDS. The novel animal models that can be directly infected with HIV-1 will contribute to develop effective strategies for eradicating infections. Here, we inoculated 4 northern pig-tailed macaques (NPM) with the HIV-1 strain HIV-1NL4.3 and monitored the infection for approximately 3 years (150 weeks). The HIV-1-infected NPMs showed transient viremia for about 10 weeks after infection. However, cell-associated proviral DNA and viral RNA persisted in the peripheral blood and lymphoid organs for about 3 years. Moreover, replication-competent HIV-1 could be successfully recovered from peripheral blood mononuclear cells (PBMCs) during long-term infection. The numbers of resting CD4+ T cells in HIV-1 infected NPMs harboring proviruses fell within a range of 2- to 3-log10 per million cells, and these proviruses could be reactivated both ex vivo and in vivo in response to co-stimulation with the latency-reversing agents JQ1 and prostratin. Our results suggested that NPMs can be infected with HIV-1 and a long-term viral reservoir was formed in NPMs, which might serve as a potential model for HIV-1 reservoir research

Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases

The genus Coronavirus contains about 25 species of coronaviruses (CoVs), which are important pathogens causing highly prevalent diseases and often severe or fatal in humans and animals. No licensed specific drugs are available to prevent their infection. Different host receptors for cellular entry, poorly conserved structural proteins (antigens), and the high mutation and recombination rates of CoVs pose a significant problem in the development of wide-spectrum anti-CoV drugs and vaccines. CoV main proteases (M(pro)s), which are key enzymes in viral gene expression and replication, were revealed to share a highly conservative substrate-recognition pocket by comparison of four crystal structures and a homology model representing all three genetic clusters of the genus Coronavirus. This conclusion was further supported by enzyme activity assays. Mechanism-based irreversible inhibitors were designed, based on this conserved structural region, and a uniform inhibition mechanism was elucidated from the structures of M(pro)-inhibitor complexes from severe acute respiratory syndrome-CoV and porcine transmissible gastroenteritis virus. A structure-assisted optimization program has yielded compounds with fast in vitro inactivation of multiple CoV M(pro)s, potent antiviral activity, and extremely low cellular toxicity in cell-based assays. Further modification could rapidly lead to the discovery of a single agent with clinical potential against existing and possible future emerging CoV-related diseases

Large-Scale Estimation of Hourly Surface Air Temperature Based on Observations from the FY-4A Geostationary Satellite

Spatially continuous surface air temperature (SAT) is of great significance for various research areas in geospatial communities, and it can be reconstructed by the SAT estimation models that integrate accurate point measurements of SAT at ground sites with wall-to-wall datasets derived from remotely sensed observations of spaceborne instruments. As land surface temperature (LST) strongly correlates with SAT, estimation models are typically developed with LST as a primary input. Geostationary satellites are capable of observing the Earth’s surface across large-scale areas at very high frequencies. Compared to the substantial efforts to estimate SAT at daily or monthly scales using LST derived from MODIS, very limited studies have been performed to estimate SAT at high-temporal scales based on LST from geostationary satellites. Estimation models for hourly SAT based on the LST derived from FY-4A, the first geostationary satellite in China’s new-generation meteorological observation mission, were developed for the first time in this study. The models were fully cross-validated for a very large-scale region with diverse geographic settings using random forest, and specified differently to explore the influence of time and location variables on model performance. Overall predictive performance of the models is about 1.65–2.08 K for sample-based cross-validation, and 2.22–2.70 K for site-based cross-validation. Incorporating time or location variables into the hourly models significantly improves predictive performance, which is also confirmed by the analysis of predictive errors at temporal scales and across sites. The best-performing model with an average RMSE of 2.22 K was utilized for reconstructing maps of SAT for each hour. The hourly models developed in this study have general implications for future studies on large-scale estimating of hourly SAT based on geostationary LST datasets

Existence of Solutions for Weighted<inline-formula> <graphic file="1029-242X-2010-751709-i1.gif"/></inline-formula>-Laplacian Impulsive Integro-Differential System Periodic-Like Boundary Value Problems

Abstract This paper investigates the existence of solutions for weighted -Laplacian impulsive integro-differential system periodic-like boundary value problems via Leray-Schauder's degree. The sufficient conditions for the existence of solutions are given.</p