Annual mean soil temperature measured at the surface and 5 cm depth at five adjacent experimental sites.

<p>HP = tea garden with high production, MP = tea garden with medium production, LP = tea garden with low production, WL = woodland, VF = vegetable field. Vertical bars indicate standard error. Bars labelled with the different lowercase letters at the same site denote significant difference at <i>p</i><0.05.</p

Seasonal change of soil temperature measured (a) at the surface and (b) at 5 cm depth, (c) soil water content in the 0–15 cm soil layer, (d) soil respiration rate at the five experimental sites.

<p>HP = tea garden with high production, MP = tea garden with medium production, LP = tea garden with low production, WL = woodland, VF = vegetable field. Vertical bars indicate standard error.</p

Annual mean soil respiration efflux in five adjacent experimental sites.

<p>HP = tea garden with high production, MP = tea garden with medium production, LP = tea garden with low production, WL = woodland, VF = vegetable field. Vertical bars indicate standard error. Bars labelled with the different lowercase letters at the same site denote significant difference at <i>p</i><0.05.</p

Variation of the soil respiration residual with temperature.

<p>HP = tea garden with high production, MP = tea garden with medium production, LP = tea garden with low production, WL = woodland, VF = vegetable field.</p

Seasonal mean precipitation and air temperature during the experimental period.

<p>Seasonal mean precipitation and air temperature during the experimental period.</p

Seasonal change of soil temperature measured (a) at the surface and (b) at 5 cm depth, (c) soil water content in the 0–15 cm soil layer, (d) soil respiration rate at the five experimental sites.

<p>HP = tea garden with high production, MP = tea garden with medium production, LP = tea garden with low production, WL = woodland, VF = vegetable field. Vertical bars indicate standard error.</p

Correlation of Q10 with the C/N ratio and pH.

<p>Correlation of Q10 with the C/N ratio and pH.</p

Evaluation of a New Cryptococcal Antigen Lateral Flow Immunoassay in Serum, Cerebrospinal Fluid and Urine for the Diagnosis of Cryptococcosis: A Meta-Analysis and Systematic Review

<div><p>Background</p><p>A new lateral flow immunoassay (LFA) for the detection of cryptococcal antigen was developed.</p><p>Objective</p><p>We aimed to systematically review all relevant studies to evaluate the diagnostic accuracy of the cryptococcal antigen LFA on serum, CSF and urine specimens.</p><p>Methods</p><p>We searched public databases including PubMed, Web of Science, Elsevier Science Direct and Cochrane Library for the English-language literature published up to September 2014. We conducted meta-analyses of sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR) and diagnostic odds ratios (DOR) and SROC of LFA in serum and CSF, respectively. The sensitivity of LFA in urine was also analyzed. Subgroup analyses were carried out to analyze the potential heterogeneity.</p><p>Results</p><p>12 studies were included in this study. The pooled sensitivity and specificity values of LFA in serum were 97.6% (95% CI, 95.6% to 98.9%) and 98.1% (95% CI, 97.4% to 98.6%), respectively. The average PLR of LFA in serum was 43.787 (95% CI, 22.60–84.81) and the NLR was 0.03 (95% CI, 0.01–0.09). The pooled DOR was 2180.30 (95% CI, 868.92–5471.00) and the AUC was 0.9968. The pooled sensitivity and specificity values of LFA in CSF were 98.9% (95% CI, 97.9% to 99.5%) and 98.9% (95% CI, 98.0% to 99.5%), respectively. The average PLR of LFA in serum was 48.83 (95% CI, 21.59–110.40) and the NLR was 0.02 (95% CI, 0.01–0.04). The pooled DOR was 2931.10 (95% CI, 1149.20–7475.90) and the AUC was 0.9974. The pooled sensitivity value of LFA in urine was 85.0% (95% CI, 78.7% to 90.1%)</p><p>Conclusions</p><p>The study demonstrates a very high accuracy of LFA in serum and CSF for the diagnosis of cryptococcosis in patients at risk. LFA in urine can be a promising sample screening tool for early diagnosis of cryptococcosis.</p></div

Subgroup analyses of LFA for the diagnosis of cryptococcal infection in serum, CSF and urine.

<p>Subgroup analyses of LFA for the diagnosis of cryptococcal infection in serum, CSF and urine.</p

Characteristics of studies included in the meta-analysis (n = 12).

<p>Abbreviations: HIV, hunman immunodeficiency virus; CM, cryptococcal meningitis; SC, suspected cryptococcosis; SM, suspected meningitis</p><p>Characteristics of studies included in the meta-analysis (n = 12).</p