Understanding the Design and Performance of Distributed Tri-Generation Systems for Home and Neighborhood Refueling

The lack of a hydrogen infrastructure remains a major barrier for hydrogen fuel cell vehicle (FCV) adoption and commercialization. The high cost of building an extensive hydrogen station network and low utilization in the near term discourages private and public investment. Innovative, distributed, small-volume hydrogen refueling methods such as home and neighborhood tri-generation systems that produce electricity and heat for buildings, as well as hydrogen for vehicles may provide a solution because the technology is available, initial capital investment is modest, and it has potential to alleviate consumer’s fuel availability concerns. This research provides analytical tools for policy makers, manufacturers and consumers to analyze tri-generation and similar energy systems in a systematic way; and to apply these tools to case studies to understand the design and technical, economic, and environmental performances of tri-generation systems for home and neighborhood refueling.These analytical tools are applied to case studies in two categories: home refueling trigeneration systems for a single-family residence; and neighborhood refueling trigeneration systems for multiple nearby households. Overall tri-generation is economically competitive with early public stations for fueling hydrogen cars

A Lexical Semantic Model of Verb-Directional Construction in Chinese - A case study of V-Shang

Master'sMASTER OF ART

Automatic Robot Hand-Eye Calibration Enabled by Learning-Based 3D Vision

Hand-eye calibration, as a fundamental task in vision-based robotic systems, aims to estimate the transformation matrix between the coordinate frame of the camera and the robot flange. Most approaches to hand-eye calibration rely on external markers or human assistance. We proposed Look at Robot Base Once (LRBO), a novel methodology that addresses the hand-eye calibration problem without external calibration objects or human support, but with the robot base. Using point clouds of the robot base, a transformation matrix from the coordinate frame of the camera to the robot base is established as I=AXB. To this end, we exploit learning-based 3D detection and registration algorithms to estimate the location and orientation of the robot base. The robustness and accuracy of the method are quantified by ground-truth-based evaluation, and the accuracy result is compared with other 3D vision-based calibration methods. To assess the feasibility of our methodology, we carried out experiments utilizing a low-cost structured light scanner across varying joint configurations and groups of experiments. The proposed hand-eye calibration method achieved a translation deviation of 0.930 mm and a rotation deviation of 0.265 degrees according to the experimental results. Additionally, the 3D reconstruction experiments demonstrated a rotation error of 0.994 degrees and a position error of 1.697 mm. Moreover, our method offers the potential to be completed in 1 second, which is the fastest compared to other 3D hand-eye calibration methods. Code is released at github.com/leihui6/LRBO.Comment: 17 pages, 19 figures, 6 tables, submitted to MSS

KCNK9 mediates the inhibitory effects of genistein on hepatic metastasis from colon cancer

Objective: The tyrosine-protein kinase inhibitor, genistein, can inhibit cell malignant transformation and has an antitumor effect on various types of cancer. It has been shown that both genistein and KNCK9 can inhibit colon cancer. This research aimed to investigate the suppressive effects of genistein on colon cancer cells and the association between the application of genistein and KCNK9 expression level. Methods: The Cancer Genome Atlas (TCGA) database was used to study the correlation between the KCNK9 expression level and the prognosis of colon cancer patients. HT29 and SW480 colon cancer cell lines were cultured to examine the inhibitory effects of KCNK9 and genistein on colon cancer in vitro, and a mouse model of colon cancer with liver metastasis was established to verify the inhibitory effect of genistein in vivo. Results: KCNK9 was overexpressed in colon cancer cells and was associated with a shorter Overall Survival (OS), a shorter Disease-Specific Survival (DFS), and a shorter Progression-Free Interval (PFI) of colon cancer patients. In vitro experiments showed that downregulation of KCNK9 or genistein application could suppress cell proliferation, migration, and invasion abilities, induce cell cycle quiescence, promote cell apoptosis, and reduce epithelial-mesenchymal transition of the colon cancer cell line. In vivo experiments revealed that silencing of KCNK9 or application of genistein could inhibit hepatic metastasis from colon cancer. Additionally, genistein could inhibit KCNK9 expression, thereby attenuating Wnt/β-catenin signaling pathway. Conclusion: Genistein inhibited the occurrence and progression of colon cancer through Wnt/β-catenin signaling pathway that could be mediated by KCNK9

Global gene expression analysis reveals reduced abundance of putative microRNA targets in human prostate tumours

<p>Abstract</p> <p>Background</p> <p>Recently, microRNAs (miRNAs) have taken centre stage in the field of human molecular oncology. Several studies have shown that miRNA profiling analyses offer new possibilities in cancer classification, diagnosis and prognosis. However, the function of miRNAs that are dysregulated in tumours remains largely a mystery. Global analysis of miRNA-target gene expression has helped illuminate the role of miRNAs in developmental gene expression programs, but such an approach has not been reported in cancer transcriptomics.</p> <p>Results</p> <p>In this study, we globally analysed the expression patterns of miRNA target genes in prostate cancer by using several public microarray datasets. Intriguingly, we found that, in contrast to global mRNA transcript levels, putative miRNA targets showed a reduced abundance in prostate tumours relative to benign prostate tissue. Additionally, the down-regulation of these miRNA targets positively correlated with the number of types of miRNA target-sites in the 3' untranslated regions of these targets. Further investigation revealed that the globally low expression was mainly driven by the targets of 36 specific miRNAs that were reported to be up-regulated in prostate cancer by a miRNA expression profiling study. We also found that the transcript levels of miRNA targets were lower in androgen-independent prostate cancer than in androgen-dependent prostate cancer. Moreover, when the global analysis was extended to four other cancers, significant differences in transcript levels between miRNA targets and total mRNA backgrounds were found.</p> <p>Conclusion</p> <p>Global gene expression analysis, along with further investigation, suggests that miRNA targets have a significantly reduced transcript abundance in prostate cancer, when compared with the combined pool of all mRNAs. The abnormal expression pattern of miRNA targets in human cancer could be a common feature of the human cancer transcriptome. Our study may help to shed new light on the functional roles of miRNAs in cancer transcriptomics.</p

Study of Resource Recovery and Epidemiology in an Anaerobic Digester

Three 4-liter packed bed anaerobic digesters were fabricated and operated at 35 degrees C, pH around 7, and hydraulic retention time (HRT) of 20, 10 and 5 days to study the resource recovery and epidemiology in a controlled ecological life support system (CELSS). A simulated wastewater, consisted of shower water, clothwash water, dishwasher water, handwash water, and urine flush water was used as the feeding solution. Under steady-state operation, chemical oxygen demand (COD), total organic carbon (TOC), pH, nitrogen, phosphorus, and potassium wer monitored in the digester input and output solutions. The volume and the CH4/CO2 ratios in the biogas produced from the anaerobic digesters were measured. The results indicate about 90 percent of TOC is converted while only 5-8 percent of N-P-K are consumed in the digester. A multi-drug resistant strain of Salmonella choleraesuis was used as the indicator bacterium in the epidemiology study. The levels of Salmonella choleraesuis in the influent and the effluent wer determined and decimal decay rate constants, k(d), were estimated. The k(d) values were greater at higher initial doses than lower doses for the same HR, and greater for batch digestion (7.89/d) than for continuous digestion (4.28, 3.82, and 3.82/d for 20, 10, and 5 d HRT, respectively)

Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease

<p>Abstract</p> <p>Background</p> <p>New strategies for the treatment of Parkinson's disease (PD) are shifted from dopamine (DA) replacement to regeneration or restoration of the nigro-striatal system. A cell therapy using human retinal pigment epithelial (RPE) cells as substitution for degenerated dopaminergic (DAergic) neurons has been developed and showed promising prospect in clinical treatment of PD, but the exact mechanism underlying this therapy is not fully elucidated. In the present study, we investigated whether the beneficial effects of this therapy are related to the trophic properties of RPE cells and their ability to synthesize DA.</p> <p>Methods</p> <p>We evaluated the protective effects of conditioned medium (CM) from cultured RPE cells on the DAergic cells against 6-hydroxydopamine (6-OHDA)- and rotenone-induced neurotoxicity and determined the levels of glial cell derived neurotrophic factor (GDNF) and brain derived neurotrophic factor (BDNF) released by RPE cells. We also measured the DA synthesis and release. Finally we transplanted microcarriers-RPE cells into 6-OHDA lesioned rats and observed the improvement in apomorphine-induced rotations (AIR).</p> <p>Results</p> <p>We report here: (1) CM from RPE cells can secret trophic factors GDNF and BDNF, and protect DAergic neurons against the 6-OHDA- and rotenone-induced cell injury; (2) cultured RPE cells express L-dopa decarboxylase (DDC) and synthesize DA; (3) RPE cells attached to microcarriers can survive in the host striatum and improve the AIR in 6-OHDA-lesioned animal model of PD; (4) GDNF and BDNF levels are found significantly higher in the RPE cell-grafted tissues.</p> <p>Conclusion</p> <p>These findings indicate the RPE cells have the ability to secret GDNF and BDNF, and synthesize DA, which probably contribute to the therapeutic effects of RPE cell transplantation in PD.</p