Multiwavelength Observations of a Flare from Markarian 501

We present multiwavelength observations of the BL Lacertae object Markarian 501 (Mrk 501) in 1997 between April 8 and April 19. Evidence of correlated variability is seen in very high energy (VHE, E > 350 GeV) gamma-ray observations taken with the Whipple Observatory gamma-ray telescope, data from the Oriented Scintillation Spectrometer Experiment of the Compton Gamma-Ray Observatory, and quicklook results from the All-Sky Monitor of the Rossi X-ray Timing Explorer while the Energetic Gamma-Ray Experiment Telescope did not detect Mrk 501. Short term optical correlations are not conclusive but the U-band flux observed with the 1.2m telescope of the Whipple Observatory was 10% higher than in March. The average energy output of Mrk 501 appears to peak in the 2 keV to 100 keV range suggesting an extension of the synchrotron emission to at least 100 keV, the highest observed in a blazar and ~100 times higher than that seen in the other TeV-emitting BL Lac object, Mrk 421. The VHE gamma-ray flux observed during this period is the highest ever detected from this object. The VHE gamma-ray energy output is somewhat lower than the 2-100 keV range but the variability amplitude is larger. The correlations seen here do not require relativistic beaming of the emission unless the VHE spectrum extends to >5 TeV.Comment: 10 pages, 2 figures, accepted for publication in ApJ Letter

Complimentary protein extraction methods increase the identification of the Park Grass Experiment metaproteome

Although the Park Grass Experiment is an important international reference soil for temperate grasslands, it still lacks the direct extraction of its metaproteome. The identification of these proteins can be crucial to our understanding of soil ecology and major biogeochemical processes. However, the extraction of protein from soil is a technically fraught process due to difficulties with co-extraction of humic material and lack of compatible databases to identify proteins. To address these issues, we combined two protein extraction techniques on Park Grass experiment soil, one based on humic acid removal, namely a modified freeze-dry, heat/thaw/phenol/chloroform (HTPC) method and another which co-extracts humic material, namely an established surfactant method. A broad range of proteins were identified by matching the mass spectra of extracted soil proteins against a tailored Park Grass proteome database. These were mainly in the categories of “protein metabolism”, “membrane transport”, “carbohydrate metabolism”, “respiration” “ribosomal and nitrogen cycle” proteins, enabling reconstitution of specific processes in grassland soil. Protein annotation using NCBI and EBI databases inferred that the Park Grass soil is dominated by Proteobacteria, Actinobacteria, Acidobacteria and Firmicutes at phylum level and Bradyrhizobium, Rhizobium, Acidobacteria, Streptomyces and Pseudolabrys at genus level. Further functional enrichment analysis enabled us to connect protein identities to regulatory and signalling networks of key biogeochemical cycles, notably the nitrogen cycle. The newly identified Park Grass metaproteome thus provides a baseline on which future targeted studies of important soil processes and their control can be built

Search for TeV Gamma-Rays from Shell-Type Supernova Remnants

If cosmic rays with energies <100 TeV originate in the galaxy and are accelerated in shock waves in shell-type supernova remnants (SNRs), gamma-rays will be produced as the result of proton and electron interactions with the local interstellar medium, and by inverse Compton emission from electrons scattering soft photon fields. We report on observations of two supernova remnants with the Whipple Observatory's 10 m gamma-ray telescope. No significant detections have been made and upper limits on the >500 GeV flux are reported. Non-thermal X-ray emission detected from one of these remnants (Cassiopeia A) has been interpreted as synchrotron emission from electrons in the ambient magnetic fields. Gamma-ray emission detected from the Monoceros/Rosette Nebula region has been interpreted as evidence of cosmic-ray acceleration. We interpret our results in the context of these observations.Comment: 4 pages, 2 figures, to appear in the proceedings of 26th International Cosmic Ray Conference (Salt Lake City, 1999

Detection of Gamma Rays with E greater than 300 GeV From Markarian 501

The detection of gamma rays of energy greater than 300 GeV from the BL Lacertae object Mrk 501 demonstrates that extragalactic TeV emission is not unique to Mrk 421. During 66 hr of observations between 1995 March and July we measured an average flux of 8.1 +/- 1.4 x 10(exp -12) cm(exp -2)/s above 300 GeV, a flux that is only 20 percent of the average Mrk 421 flux. The new gamma-ray source has not been reported by the Compton Gamma Ray Observatory as an emitter of gamma rays at lower energies. There is evidence for variability on timescales of days

TeV Observations of the Variability and Spectrum of Markarian 501

Markarian 501 is only the second extragalactic source to be detected with high statistical certainty at TeV energies; it is similar in many ways to Markarian 421. The Whipple Observatory gamma-ray telescope has been used to observe the AGN Markarian 501 in 1996 and 1997, the years subsequent to its initial detection. The apparent variability on the one-day time-scale observed in TeV gamma rays in 1995 is confirmed and compared with the variability in Markarian 421. Observations at X-ray and optical wavelengths from 1997 are also presented.Comment: 4 pages, 2 figures, to appear in proceedings of 25th ICRC (Durban