Determination Of N-Acetylcysteine In The Presence Of Ciprofloxacin Or Levofloxacin In Microparticulate Dry Powder Inhalers

A fast and easy method was validated for simultaneous determination of ciprofloxacin hydrochloride mono hydrate (CP), levofloxacin hemihydrate (LV), and N-acetylcysteine (NAC) in samples. The analysis was performed on a C-18 column (250 x 4.6 mm, 5 mu m) (Inertsil ODS-3V) using an isocratic elution method with a mobile phase composed of 25 mM KH2 PO4 (pH 3.0) and methanol (72:28, v/v) at a flow rate of 1 mL/min. UV detection was performed at 214 nm for NAC and 293 nm for CP and LV. The method was validated for linearity, accuracy, precision (repeatability and reproducibility), specificity, sensitivity, and stability. The calibration study using several media demonstrated that the calibration curves were linear for all compounds in all media (R-2 > 0.9993). The limit of detection was 0.098 mu g/mL for CP, 0.049 mu g/mL for LV, and 0.487 mu g/mL for NAC. The limit of quantification was 0.328 mu g/mL for CP, 0.165 mu g/mL for LV, and 1.624 mu g/mL for NAC. Precision and accuracy values of the method fulfilled the required limits. All these outcomes demonstrate that the validated HPLC method is appropriate for simultaneous analysis of CP, LV, and NAC in samples for content uniformity of dry powder inhaler and permeability studies.WoSScopu

THE VALUE OF LABIAL BIOPSY IN THE DIFFERENTIAL DIAGNOSIS OF SARCOIDOSIS AND SJOGREN'S SYDROME AND IMMUNOHISTOCHEMICAL ANALYSIS

The aim of this study was the evaluation of the value of labial biopsy in the differential diagnosis of sarcoidosis and Sjogren's Syndrome and the value of immunohistochemical markers such as CD3, CD4, CD8, and CD20 at the immunopathogenesis. Fifteen labial salivary gland biopsies from patients affected by primary Sjogren's syndrome, and 15 labial salivary gland biopsies from patients affected by sarcoidosis, were included in the study. In all patients, biopsies were carried out as a diagnostic procedure either for sicca syndrome or sarcoidosis

Effect Of Particle Size And Surfactant On The Solubility, Permeability And Dissolution Characteristics Of Deferasirox

Deferasirox is an oral iron chelator used for the treatment of chronic iron overload in blood transfusions. Deferasirox is a BCS Class II drug with low solubility and high permeability. In the formulation development stage for BCS Class II compounds, one of the main approaches is solubility enhancement to achieve better dissolution profiles, increased bioavailability and in some cases, dose reduction. The aim of the study was to investigate the effect of particle size and surfactant on the solubility, permeability and dissolution characteristics of deferasirox. Ball milling method was used to reduce the particle size of deferasirox. Pluronic F127 or sodium lauril sulfate (SLS) were selected as surfactants at different concentrations. The maximum increase in the solubility was obtained with 10% SLS at pH 1.2 (from 0.9 mu g/mL to 333.7 mu g/mL), and with 5% Pluronic F127 at pH 6.8 (from 46.8 mu g/mL to 334.2 mu g/mL). Dissolution studies revealed that time to dissolve 85% of deferasirox was decreased as a function of ball milling time and particle size. Permeability studies showed that, in 100 mu M concentration, deferasirox permeability was significantly enhanced by all concentrations of SLS (p0.05). All these results clearly demonstrated that surfactant addition to the formulations was effective for solubility enhancement of deferasirox, and surfactant type in optimized concentrations was very crucial. Particle size reduction can be used as a promising approach to improve dissolution, and hence bioavailability of deferasirox.WoSScopu

Evaluation of preparation methods for orally disintegrating tablets

Oral disintegrating tablets (ODT) are orally administered solid dosage forms commonly used in pediatric and geriatric patients with difficulty in swallowing. The lack of need for water during the use of ODTs is another advantage that increases patient compliance. Many methods are used for the production of ODTs such as direct compression (DC), freeze-drying (FD), spray drying, 3-D printing, melt granulation, phase transition process, molding, sublimation, mass extrusion, cotton candy process. Since the ODTs produced are aimed to disintegrate and dissolve rapidly, and consequently act quickly, the production method parameters need to be optimized in line with the critical product parameters. In this study, the most widely used manufacturing methods (especially DC and FD) for ODT and in vitro quality control tests of ODT are evaluated. [Med-Science 2020; 9(1.000): 265-9

Surgical treatment of Peyronie's disease: A critical analysis

Objective: The present paper reviews surgical treatment alternatives for patients with Peyronie's disease using knowledge obtained from the contemporary literature

Pulmonary Anaplastic Large Cell Lymphoma - a Rare Case

Anaplastic large cell lymphoma (ALCL) is a rare NHL, representing only 2-3% of all lymphomas. Pulmonary involvement is rare (5-15%). A thirty one year old female was admitted to a center with purulent fistulized lesions on the neck and axilla and enlargement of the breasts. There was no improvement with antibiotics and she had a fever. Pyogenic granulation was detected in the soft tissue biopsy of the axillary and breast. All the cultures were negative. Antituberculosis therapy was given for three months. This patient was admitted to our clinic due to clinical progression. Bilaterally painful, purulent flowing lesions on the neck and axilla, tension and sensitive breasts and high fever were found. Anemia, thrombocytosis, neutrophilic leukocytosis, low iron level and iron binding capacity and high CRP levels were detected in the laboratory tests. Anaerobic, actinomycosis, mycobacteria, nocardia and tularemia cultures were negative. Thorax CT showed a mediastinal conglomerate LN, left upper apicoposterior cavitary lesion and millimetric nodules. Abdominal CT was normal. Neck CT showed masses which erased the fat plain and submandibular LN. LN biopsy diagnosed an inflammatory variant of CD30(+) ALCL. It was considered to be stage IV due to pulmonary parenchyma, mediastinum, neck, axilla and breast involvement. After five chemotherapy sessions, there was a significant improvement in the lesions

Lymphoma and Pulmonary Involvement in Primary Sjogren's Syndrome: A Case Report

Here, we report a case of nodal marginal zone lymphoma in primary Sjogren's syndrome (SS) presenting with findings of pulmonary involvement. A 46-year-old woman was admitted to our hospital with fatigue, cough, dyspnea, xerostomia and weight loss. On physical examinations cervical and supraclavicular lymphadenopathies were noted. Chest X-P and computed tomographic films demonstrated reticular shadows in the bilateral lower lung and transbronchial lung biopsy revealed desquamative interstitial pneumonitis (DIP). Although immunoserological tests were negative, this patient was diagnosed as primary SS by sicca symptoms, positive Schirmer tear test and labial gland biopsy. Excisional biopsy of cervical lymph node revealed the presence of nodal marginal zone lymphoma

Exon 2: Is it the good police in familial mediterranean fever?

WOS: 000463722100007PubMed ID: 30489254Objective: Familial Mediterranean fever (FMF) is the most common autoinflammatory disease. Most of the identified disease-causing mutations are located on exon 10. As the number of studies about the effect of the exonal location of the mutation and its phenotypic expression is limited, we aimed to investigate whether the exonic location of the Mediterranean fever (MEFV) mutation has an effect on the clinical manifestation in patients with FMF. Methods: Study population was derived from the main FMF registry that included 2246 patients from 15 different rheumatology clinics. We categorized the mutations according to their exon locations and retrieved the clinical and demographic information from the database. Results: Patients having the MEFV mutations on exon 2 or 10 (n: 1526) were divided into three subgroups according to the location of the MEFV mutations: Group 1 (exon 2 mutations), Group 2 (exon 10 mutations), and Group 3 (both exon 2 and exon 10 mutations). Group 2 patients were of a significantly younger age at onset, and erysipel-like erythema, arthritis, amyloidosis, and a family history of FMF were more common in this group. Conclusion: Patients with FMF and exon 10 mutations show more severe clinical symptoms and outcome. Exon 2 mutations tend to have a better outcome