Effects of WY14643 and fenofibrate on acetylcholine-induced contractions in aortic rings from spontaneously hypertensive rats

Conference Theme: Bridging Translational GapChaired Posters: no. CP5INTRODUCTION AND OBJECTIVE: Oxidative stress is implicated in the release of endothelium-derived contracting factors, the release of which is associated with endothelial dysfunction. Peroxisome proliferator-activated receptor (PPAR) –alpha agonists, Wy14643 (WY) and fenofibrate (FF) have been found improving endothelial function in cardiovascular diseases. The present study was designed to examine whether or not WY and FF inhibit endothelium-dependent contractions through reduction of free ...postprin

Genistein acutely potentiates acetylcholine-induced relaxation through a G-protein coupled pathway in spontaneously hypertensive rats

Abstracts for Oral Communications: OC5OBJECTIVES: Genistein, a phytoestrogen rich in soy beans and soy products, was reported to be a vasorelaxant. This study examined the receptor and related signaling pathways in the ...published_or_final_versionThe 12th Annual Scientific Meeting of Institute of the Cardiovascular Science and Medince (ICSM), Hong Kong, 13-14 December 2008. In Journal of the Hong Kong College of Cardiology, 2008, v. 16 n. 2, p. 59, abstract no. OC

Epac1-selective cyclic-AMP analog 8-pCPT-2'-O-Me-cAMP induces vasodilation on porcine coronary artery in endothelium-independent pathway

BACKGROUND: Exchange protein directly activated by cyclic AMP (cAMP) 1, Epac1, is a newly discovered cAMP target which acts beyond the classical cAMP effector protein kinase A (PKA). The identification of Epac1 overthrows the concept that cAMP activatez single effector. Epac1 is a guanine nucleotide exchange factor (GEF) probably activates small G-protein Rap1. Epac1 ubiquitously expresses in all tissues and therefore should play important roles in cellular signaling. Appreciation of the potential importance of Epac1 has come with the current development of Epac1-selective-cAMP analogs for discriminating the activation from PKA to Epac1. Among those analogs …postprintThe 2011 Winter Meeting of the British Pharmacological Society (BPS), London, U.K., 13-15 December 2011, abstract no. P04

Wy14643 and fenofibrate reduce contractions to hydrogen peroxide in aortae of spontaneously hypertensive rats

The First joint meeting between the Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists (ASCEPT) and the British Pharmacological Society (BPS) , in association with the Hong Kong Pharmacology Society (HKPS) and the Asia Pacific Federation of Pharmacologists (APFP).Conference Theme: Tomorrow's medicines: pharmacology, patients and populationspublished_or_final_versio

1,25-Dihydroxyvitamin D3 acutely reduces acetylcholine-induced endothelium-dependent contraction in hypertensive rat aorta through activation of protein kinase C

This journal suppl. entitled: EDHF 2012 - 10th Anniversary MeetingVitamin D derivatives affect the regulation of cardiovascular system. Recent study from our lab indicated that 1,25-dihydroxyvitamin D3, the major metabolite of vitamin D, acutely reduced endothelium-dependent contraction to acetylcholine in spontaneously hypertensive rats (SHR) aorta. This reduction was associated with reduced cytosolic-free calcium in endothe...postprin

Vascular nitric oxide: Beyond eNOS

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Effect of isoflavones on the vascular actions of phosphodiesterase inhibitors

This journal suppl. entitled: EDHF 2012 - 10th Anniversary MeetingIsoflavones are present in high concentrations in soy products, the intake of which is associated with lower incidence of cardiovascular diseases. They can enhance contraction and inhibit relaxation through cyclic adenosine monophosphate (cAMP)-dependent pathway. The present study aimed to examine whether or not the major soy isoflav...postprin

Concentration-dependent vascular effects of divalent manganese

Oral Presentation: no. OP9OBJECTIVES: Divalent manganese is a cofactor for soluble guanylyl cyclase (sGC), the enzyme producing cyclic GMP in vascular smooth muscle cells that causes relaxation. Manganese competes with magnesium to activate sGC. These divalent cations can bias the activity of the enzyme to produce cyclic nucleotides other than cyclic GMP, in particular cyclic AMP and cyclic IMP. Cyclic IMP, preferably produced in the presence of magnesium, can cause contraction in ...postprin

Antiretroviral treatment can affect the release of NO and EDCF, but EDH in rat arteries

This journal suppl. entitled: EDHF 2012 - 10th Anniversary MeetingDespite improving clinical outcomes, highly active antiretroviral therapy (HAART) is an independent potential risk factor for cardiovascular diseases. Currently the recommended HAART regimen commonly comprises a protease inhibitor (PI) with ritonavir (RTV)-boosting or a nonnucleoside reverse transcriptase inhibitor (NNRTI), and two nucleoside reverse transcriptase inhibitors. The present study examined whether or not boosted lopin...postprin

siRNA Versus miRNA as Therapeutics for Gene Silencing

Discovered a little over two decades ago, small interfering RNAs (siRNAs) and microRNAs (miRNAs) are noncoding RNAs with important roles in gene regulation. They have recently been investigated as novel classes of therapeutic agents for the treatment of a wide range of disorders including cancers and infections. Clinical trials of siRNA- and miRNA-based drugs have already been initiated. siRNAs and miRNAs share many similarities, both are short duplex RNA molecules that exert gene silencing effects at the post-transcriptional level by targeting messenger RNA (mRNA), yet their mechanisms of action and clinical applications are distinct. The major difference between siRNAs and miRNAs is that the former are highly specific with only one mRNA target, whereas the latter have multiple targets. The therapeutic approaches of siRNAs and miRNAs are therefore very different. Hence, this review provides a comparison between therapeutic siRNAs and miRNAs in terms of their mechanisms of action, physicochemical properties, delivery, and clinical applications. Moreover, the challenges in developing both classes of RNA as therapeutics are also discussed.published_or_final_versio