Isolation, morphological characterization, and screening virulence of Beauveria bassiana and Metarhizium robertsii fungal isolates in Galleria mellonella [version 4; peer review: 2 approved]

Background Entomopathogenic fungi exists naturally in plants as an asymptote and have the potential to reduce the population of insect pests through indirect interactions. This study was conducted to detect and characterize the endophytic fungi Beauveria bassiana and Metarhizium robertsii from the rhizosphere soil of tomato plants and their virulence effect on Galleria melonella. Methods From the rhizosphere soil of 40 tomato fields, three Beauveria bassiana and seven Metarhizium robertsii isolates were isolated using the galleria bait method. All fungi isolate were morphologically characterized by their colony color, shape, and surface texture. Isolates with the highest percentages of germination, conidial yield, and radial growth were selected, and their virulence was evaluated on second instar larvae of Galleria melonella under laboratory conditions. Results In this study, Beauveria bassiana showed white colony color and aseptate hyphae, whereas Metarhizium robertsii showed dark green to light green colony color and septate hyphal structures. Maximum spore production and conidial length were obtained by Beauveria bassiana isolate APPRC-27 with 2.67x107 spores ml-1 and 3.24 µm, respectively. Colony radial growth rates ranged from 1.73 to 3.24 mm day-1. The results revealed that the highest mortality rate of Galleria melonella (100%) was obtained by Metarhizium robertsii isolates K-61 and K-102 at a concentration of 1x108 conidial ml-1 at 7 days post-inoculation. The lowest mortality rate was registered by Metarhizium robertsii isolate RST-11. Conclusions In the present study, isolates that produced the most spores and had the highest germination rates were the most virulent to Galleria mellonella second instar larvae. Therefore, virulent entomopathogenic fungi, Beauveria bassiana and Metarhizium robertsii, are promising bioagents for the control of insect pests

Determinants of Child Labour and Schooling in Rural Households of Ethiopia:

Parallel to other low-income countries, child labour remains a multi-dimensional grave problem and policy issue in Ethiopia. It has long been recognized as an important barrier to national human capital development and thus magnifies the risks of turning a country’s most prized assets into its biggest liabilities. This study attempts to comprehensively investigate the various determinants of child labour and schooling for children aged 7 to 17 using the data collected from rural households of Kuyu woreda, Oromia Regional National State. The study employed multinomial logit model to achieve the main objectives. The results from the empirical analysis suggest that socio-economic characteristics of both child and household are found to be significant determinants of child labour and school participation among rural households of Kuyu woreda. The study has revealed the existence of positive and significant relationship between child school and being the direct off-spring of the household head, the household head education attainment and being free of recurrent health shocks of the household head, whereas negative association between child school, and age and sex of the child, the presence of female child aged between 7 and 17 in the household and loose of livestock. Besides, it has also revealed the existence of positive and significant relationship between child work, and age of the child and household size, whereas, negative association between sex and birth order of the child, sex of the household head, household land size and inaccessibility to welfare programs. From policy perspectives, improving the livelihood of rural communities, extending rural communities’ access to welfare programs, developing micro-insurance programs including micro-health insurance programs and working in collaboration were recommended to hold back child labour in turn enhancing child schooling

Application of Linear Programming to Analyze Profit of Flour Factory, in the Case of Sanate Flour Factory, at Robe Town

The purpose of this study was to analyze the total production and profit of Sanate flour factory located in Ethiopia, Oromia regional state, Bale zone, Robe town, by applying linear programming. A factory is situated within Robe town about 430 KM, from Addis Ababa (Capital of Ethiopia). Today linear programming was the most popular method of manipulating a large amount of data. Hence, Different studies bring out the necessity of using quantitative techniques for utilization in the factory. So, in this paper to analyze the production and profit of this factory, the study incorporates different steps; the first step is collecting data. A data collecting formats prepared and circulated among factory staff to executive managers, co-managers, sellers, machine operators, and technicians to determine the production, sales, and profit during five months of November 30, 2018- June 18, 2019. In the second step, a collected data is modeled to mathematical form, particularly modeled to linear program. In the third step the mathematical modeled data was solved (analyzed). Finally, depending on the empirical results (the solution of a modeled data) some problem facing the factory was indicated and the solution for the problem has been recommended

Longitudinal profile of antibody response to SARS-CoV-2 in patients with COVID-19 in a setting from Sub-Saharan Africa: A prospective longitudinal study.

BACKGROUND Serological testing for SARS-CoV-2 plays an important role for epidemiological studies, in aiding the diagnosis of COVID-19, and assess vaccine responses. Little is known on dynamics of SARS-CoV-2 serology in African settings. Here, we aimed to characterize the longitudinal antibody response profile to SARS-CoV-2 in Ethiopia. METHODS In this prospective study, a total of 102 PCR-confirmed COVID-19 patients were enrolled. We obtained 802 plasma samples collected serially. SARS-CoV-2 antibodies were determined using four lateral flow immune-assays (LFIAs), and an electrochemiluminescent immunoassay. We determined longitudinal antibody response to SARS-CoV-2 as well as seroconversion dynamics. RESULTS Serological positivity rate ranged between 12%-91%, depending on timing after symptom onset. There was no difference in positivity rate between severe and non-severe COVID-19 cases. The specificity ranged between 90%-97%. Agreement between different assays ranged between 84%-92%. The estimated positive predictive value (PPV) for IgM or IgG in a scenario with seroprevalence at 5% varies from 33% to 58%. Nonetheless, when the population seroprevalence increases to 25% and 50%, there is a corresponding increases in the estimated PPVs. The estimated negative-predictive value (NPV) in a low seroprevalence scenario (5%) is high (>99%). However, the estimated NPV in a high seroprevalence scenario (50%) for IgM or IgG is reduced significantly to 80% to 85%. Overall, 28/102 (27.5%) seroconverted by one or more assays tested, within a median time of 11 (IQR: 9-15) days post symptom onset. The median seroconversion time among symptomatic cases tended to be shorter when compared to asymptomatic patients [9 (IQR: 6-11) vs. 15 (IQR: 13-21) days; p = 0.002]. Overall, seroconversion reached 100% 5.5 weeks after the onset of symptoms. Notably, of the remaining 74 COVID-19 patients included in the cohort, 64 (62.8%) were positive for antibody at the time of enrollment, and 10 (9.8%) patients failed to mount a detectable antibody response by any of the assays tested during follow-up. CONCLUSIONS Longitudinal assessment of antibody response in African COVID-19 patients revealed heterogeneous responses. This underscores the need for a comprehensive evaluation of seroassays before implementation. Factors associated with failure to seroconvert needs further research

Prevalence of Trachoma in Pre-validation Surveillance Surveys in 11 Evaluation Units (Covering 12 Districts) in Oromia Regional State, Ethiopia: Results from 2018−2020

PURPOSE: Interventions to reduce the prevalence of trachoma and transmission of ocular Chlamydia trachomatis have been implemented in Oromia Region, Ethiopia. Following an impact survey in which the trachomatous inflammation—follicular (TF) prevalence in 1–9-year-olds is <5%, a surveillance survey is recommended 2 years later, without additional antibiotic treatment. We report results of surveillance surveys in 11 evaluation units (EUs) covering 12 districts in Oromia Region, to plan whether future interventions are needed. METHOD: We use a two-stage cluster-sampling cross-sectional survey design. In each EU, 26 clusters (villages) were systematically selected with probability proportional to size; from each cluster, 30 households were selected using compact segment sampling. Water, sanitation and hygiene (WASH) access was assessed in all selected households. All residents of selected households aged ≥1 year were examined for TF and trachomatous trichiasis (TT) by certified graders. RESULT: Of 31,991 individuals enumerated, 29,230 (91% of) individuals were examined. Eight EUs had an age-adjusted TF prevalence in 1−9-year-olds of ≥5% and seven had a TT prevalence unknown to the health system among adults aged ≥15 years of ≥0.2%. About one-third of visited households had access to an improved water source for drinking, and 5% had access to an improved latrine. CONCLUSION: Despite TF reductions to <5% at impact survey, prevalence recrudesced to ≥5% in all but three of the 11 EUs. Operational research is needed to understand transmission dynamics and epidemiology, in order to optimise elimination strategies in high-transmission settings like these

Prevalence of Trachoma after Implementation of Trachoma Elimination Interventions in Oromia Regional State, Ethiopia: Results of Impact Surveys in 131 Evaluation Units Covering 139 Districts

PURPOSE: To determine the prevalence of trachomatous inflammation—follicular (TF), trachomatous trichiasis (TT), water, sanitation, and hygiene (WASH) access in 131 evaluation units (EUs) after implementation of trachoma elimination interventions in Oromia Region, Ethiopia. METHODOLOGY: A population-based cross-sectional survey was conducted in each EU using the World Health Organization-recommended two-stage cluster-sampling methodology. Twenty-six clusters, each with a mean of 30 households were enumerated in each EU. All residents aged ≥1 year in selected households were examined for TF and TT. Information on WASH access in surveyed households was also collected through questioning the household head and direct observation. RESULTS: A total of 419,858 individuals were enumerated in 131 EUs, of whom 396,134 (94%) were examined, 54% being female. Age-adjusted EU-level prevalence of TF in children aged 1–9 years ranged from 0.15% (95% confidence interval [CI]: 0.0–0.4) to 37.5% (95% CI: 31.1–43.7). The TF prevalence was <5% in 73/131 (56%) EUs. The EU-level age- and gender-adjusted prevalence of TT unknown to the health system among people aged ≥15 years ranged from 0.001% (95% CI: 0.00–0.02) to 2.2% (95% CI: 1.1–3.1) with 37/131 (28%) EUs having a prevalence <0.2%. Only 48% of all households surveyed had access to improved water sources for drinking. Approximately 96% of households did not have an improved latrine. CONCLUSION: Oromia is on the path towards elimination of trachoma as a public health problem

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance.

Investment in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing in Africa over the past year has led to a major increase in the number of sequences that have been generated and used to track the pandemic on the continent, a number that now exceeds 100,000 genomes. Our results show an increase in the number of African countries that are able to sequence domestically and highlight that local sequencing enables faster turnaround times and more-regular routine surveillance. Despite limitations of low testing proportions, findings from this genomic surveillance study underscore the heterogeneous nature of the pandemic and illuminate the distinct dispersal dynamics of variants of concern-particularly Alpha, Beta, Delta, and Omicron-on the continent. Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve while the continent faces many emerging and reemerging infectious disease threats. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

The evolving SARS-CoV-2 epidemic in Africa: Insights from rapidly expanding genomic surveillance

INTRODUCTION Investment in Africa over the past year with regard to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sequencing has led to a massive increase in the number of sequences, which, to date, exceeds 100,000 sequences generated to track the pandemic on the continent. These sequences have profoundly affected how public health officials in Africa have navigated the COVID-19 pandemic. RATIONALE We demonstrate how the first 100,000 SARS-CoV-2 sequences from Africa have helped monitor the epidemic on the continent, how genomic surveillance expanded over the course of the pandemic, and how we adapted our sequencing methods to deal with an evolving virus. Finally, we also examine how viral lineages have spread across the continent in a phylogeographic framework to gain insights into the underlying temporal and spatial transmission dynamics for several variants of concern (VOCs). RESULTS Our results indicate that the number of countries in Africa that can sequence the virus within their own borders is growing and that this is coupled with a shorter turnaround time from the time of sampling to sequence submission. Ongoing evolution necessitated the continual updating of primer sets, and, as a result, eight primer sets were designed in tandem with viral evolution and used to ensure effective sequencing of the virus. The pandemic unfolded through multiple waves of infection that were each driven by distinct genetic lineages, with B.1-like ancestral strains associated with the first pandemic wave of infections in 2020. Successive waves on the continent were fueled by different VOCs, with Alpha and Beta cocirculating in distinct spatial patterns during the second wave and Delta and Omicron affecting the whole continent during the third and fourth waves, respectively. Phylogeographic reconstruction points toward distinct differences in viral importation and exportation patterns associated with the Alpha, Beta, Delta, and Omicron variants and subvariants, when considering both Africa versus the rest of the world and viral dissemination within the continent. Our epidemiological and phylogenetic inferences therefore underscore the heterogeneous nature of the pandemic on the continent and highlight key insights and challenges, for instance, recognizing the limitations of low testing proportions. We also highlight the early warning capacity that genomic surveillance in Africa has had for the rest of the world with the detection of new lineages and variants, the most recent being the characterization of various Omicron subvariants. CONCLUSION Sustained investment for diagnostics and genomic surveillance in Africa is needed as the virus continues to evolve. This is important not only to help combat SARS-CoV-2 on the continent but also because it can be used as a platform to help address the many emerging and reemerging infectious disease threats in Africa. In particular, capacity building for local sequencing within countries or within the continent should be prioritized because this is generally associated with shorter turnaround times, providing the most benefit to local public health authorities tasked with pandemic response and mitigation and allowing for the fastest reaction to localized outbreaks. These investments are crucial for pandemic preparedness and response and will serve the health of the continent well into the 21st century

Groundwater Potential Assessment Using GIS and Remote Sensing Techniques: Case Study of West Arsi Zone, Ethiopia

Groundwater is a crucial source of water supply due to its continuous availability, reasonable natural quality, and being easily diverted directly to the poor community more cheaply and quickly. The West Arsi Zone residents remain surface water dependent due to traditional exploration of groundwater, which is a tedious approach in terms of resources and time. This study uses remote sensing data and geographic information system techniques to evaluate the groundwater potential of the study area. This technique is a fast, accurate, and feasible technique. Groundwater potential and recharge zone influencing parameters were derived from Operational Land Imager 8, digital elevation models, soil data, lithological data, and rainfall data. Borehole data were used for results validation. With spatial analysis tools, the parameters affecting groundwater potential (LULC, soil, lithology, rainfall, drainage density, lineament density, slope, and elevation) were mapped and organized. The weight of the parameters according to percent of influence on groundwater potential and recharge was determined by Analytical Hierarchy Process according to their relative influence. For weights allocated to each parameter, the consistency ratio obtained was 0.033, which is less than 0.1, showing the weight allocated to each parameter is acceptable. In the weighted overlay analysis, from a percent influence point of view, slope, land use/cover, and lithology are equally important and account for 24% each, while the soil group has the lowest percent of influence, which accounts only 2% according to this study. The generated groundwater potential map has four ranks, 2, 3, 4, and 5, in which its classes are Low, Moderate, High, and Very High, respectively, based on its groundwater potential availability rank and class. The area coverage is 9825.84 ha (0.79%), 440,726.49 ha (35.46%), 761,438.61 ha (61.27%), and 30,748.68 ha (2.47%) of the study area, respectively. Accordingly, the western part of district is expected to have very high groundwater potential. High groundwater potential is concentrated in the central and western parts whereas moderate groundwater potential distribution is dominant in the eastern part of the area. The validation result of 87.61% confirms the very good agreement among the groundwater record data and groundwater potential classes delineated

Groundwater Potential Assessment Using GIS and Remote Sensing Techniques: Case Study of West Arsi Zone, Ethiopia

Groundwater is a crucial source of water supply due to its continuous availability, reasonable natural quality, and being easily diverted directly to the poor community more cheaply and quickly. The West Arsi Zone residents remain surface water dependent due to traditional exploration of groundwater, which is a tedious approach in terms of resources and time. This study uses remote sensing data and geographic information system techniques to evaluate the groundwater potential of the study area. This technique is a fast, accurate, and feasible technique. Groundwater potential and recharge zone influencing parameters were derived from Operational Land Imager 8, digital elevation models, soil data, lithological data, and rainfall data. Borehole data were used for results validation. With spatial analysis tools, the parameters affecting groundwater potential (LULC, soil, lithology, rainfall, drainage density, lineament density, slope, and elevation) were mapped and organized. The weight of the parameters according to percent of influence on groundwater potential and recharge was determined by Analytical Hierarchy Process according to their relative influence. For weights allocated to each parameter, the consistency ratio obtained was 0.033, which is less than 0.1, showing the weight allocated to each parameter is acceptable. In the weighted overlay analysis, from a percent influence point of view, slope, land use/cover, and lithology are equally important and account for 24% each, while the soil group has the lowest percent of influence, which accounts only 2% according to this study. The generated groundwater potential map has four ranks, 2, 3, 4, and 5, in which its classes are Low, Moderate, High, and Very High, respectively, based on its groundwater potential availability rank and class. The area coverage is 9825.84 ha (0.79%), 440,726.49 ha (35.46%), 761,438.61 ha (61.27%), and 30,748.68 ha (2.47%) of the study area, respectively. Accordingly, the western part of district is expected to have very high groundwater potential. High groundwater potential is concentrated in the central and western parts whereas moderate groundwater potential distribution is dominant in the eastern part of the area. The validation result of 87.61% confirms the very good agreement among the groundwater record data and groundwater potential classes delineated.</jats:p