Penyimpanan in Vitro Tanaman Obat Daun Dewa Melalui Pertumbuhan Minimal

Daun dewa (Gynura Procumbens) is a medicinal crop commonly used to remedy cancer, diabetes, and dermatitis. It has a bright prospect for future used. Plant preservations through tissue culture is done to anticipate an urgent need. An experiment was carried out to in vitro preservation of Daun Dewa by the minimum growth and regeneration to examine viability of the culture after the preservation. Terminal shoots (±1 cm) were cultured on a 1/2 MS basic medium + paclobutrazol (0, 1, 2, 3, and 4 mg/l) or ABA (1, 2, and 5 mg/l). The trial was arranged in a, completely randomized with 10 replications. The results showed a three-month preservation of the culture on a medium containing ABA inhibited proliferation and expansion of the plant shoots. Increasing ABA concentrations up to 5 mg/l, according to the shoot-growth inhibition, resulted in the height of 0.6 cm. After three month preservation, the shoots were able to produce roots. After 12 month preservation, the optimum capacity of growth inhibition was shown on 1/2 MS medium + ABA (1, 3, and 5 mg/l). The application of paclobutrazol (1, 2, 3, and 4 mg/l) in the medium produced low multiplication level of shoots, the length of the shoots remains higher than those on 1/2 MS medium without paclobutrazol. Seven months after preservation, viability of the plants was still high when cultured on MS medium + 2 mg/l BA combined with paclobutrazol and ABA as previously given. In addition, the rooted culture could be directly acclimatized in the glasshouse. The lowest number of shoot and shortest shoot after 12 month preservation period was found on the medium containing 5 mg/l ABA and 4 mg/l paclobutrazol, this treatment produced two shoots of 4 cm long. The best medium for the explant regeneration after 7 month preservation was MS + 2 mg/l BA. The plant shoots produced roots directly after they were acclimatized in the glasshouse

Induksi Kalus dan Regenerasi Tunas Padi Varietas Fatmawati

Efficiency of shoot regeneration from rice callus explant is an important factor, particularly for the purpose of plant genetic improvement such as somaclonal variation, transformation and in vitro selection. In the attempt of gaining shoot regeneration, callus induction and shoot regeneration experiments were conducted in the Biological Cell and Tissue Culture Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development. Mature seed was used as an explant. The media formulation for calli induction was MS + 2.4-D (1, 3, 5 and 7 mg/l ) + prolin (0 and 100 mg/l ) and hydrolyzed casein 3000 mg/l. The media for shoot regeneration was (1) MS + BA (1.2 and 3 mg/l ) + IAA 0.8 mg/l + prolin 100 mg/l, and (2) MS + BA 2 mg/l + IAA 0.8 mg/l + zeatin (0.1; 0.2 and 0.3 mg/l). The result showed that the embryogenic calli could be produced from MS + 2.4-D 3 mg/l + hydrolyzed casein 3000 mg/l treatment and the best media for shoot induction was MS + BA 2 mg/l + IAA 0.8 mg/l + zeatin 0.2 mg/l. The obtained plantlets were successfully acclimatized in the greenhouse

Analisis Kepatuhan Perawat pada Standar Asuhan Keperawatan di Unit Rawat Inap Kelas III RSU PKU Muhammadiyah Bantul YOGYAKARTA Tahun 2010

Background: A hospital is an organization of health professionals through an organized and permanent medical facilities conducting medical services, continuous nursing care, diagnosis and treatment of illnesses suffered by patients. Nurses require documentation standards as guidance and direction in maintenance recording / documentation of activities and instructions for making patterns / recording formats appropriate, evidence recording and reporting on the status of the client and used as a communication tools that is accurate and complete. Nurses are not only required to improve the quality of nursing care, but also claimed to be properly documented. Nurses often do not obey and do the routine without regard to their legality aspect, although many factors are influence documentation. This study aims to determine compliance with standards of nursing care nurse at PKU Muhammadiyah Hospital in Bantul. Methods: This was a qualitative descriptive research with a view to charging documents compliance with nursing standards of nursing care PKU Muhammadiyah Hospital in Bantul. This research was conducted with a view of nursing care standards document in May 2010. Implementation process that includes data collection, data processing, and presentation of comprehensive data and information accurately, timely, and trustworthy in the hospitalization. Results: he results seen from the documentation of nursing care standards shows that the nurses in the inpatient unit class PKU Muhammadiyah Hospital in Bantul III comply with the standards of nursing care that is 100% obedient to categorize nurses in assessment, diagnosis and planning, while 94.7% of nurses in the implementation of compliance and categorize 89.5% nurses in the evaluation categorize obedient. Conclusion: Compliance with the standards of nursing care nurse in the Inpatient Unit RSU PKU Muhammadiyah class III Bantul, has been running quite well because the nurse dutifully on the charging document nursing care standards

Asuhan Kebidanan pada Ibu Bersalin dengan Rangsangan Puting Susu di Bpm Lilik Kustono Diwek Jombang

  Pendahuluan: Proses persalinan normal ditentukan oleh tiga faktor utama, yaitu power (his dan tenaga mengejan), passanger (janin, plasenta dan selaput ketuban) dan passage (jalan lahir). Ketiga faktor utama ini sangat menetukan jalannya persalinan. Upaya untuk meningkatkan kontraksi dapat dilakukan secara farmakologis dan non farmakologis. Salah satu upaya untuk menigkatkan kontraksi non farmakologis yaitu dengan stimulasi puting susu. WHO memperkirakan 70% mengalami peningkatan kontraksi uterus setelah dilakukan stimulasi dan 30% tidak mengalami peningkatan karena kurangnya penanganan gerakan putar-putar puting susu. Dinas Kesehatan Jawa Timur melaporkan adanya peningkatan karena rangsangan puting susu sebesar 29 orang atau 380/100.000 kelahiran hidup pada tahun 2010. Laporan studi kasus ini bertujuan untuk melaksanakan asuhan kebidanan pada ibu bersalin dengan rangsangan puting susu di BPM Lilik Kustono, Amd.Keb Desa Ceweng. Metode: Jenis penelitian studi kasus, lokasi studi kasus di BPM Lilik Kustono, Amd.Keb Desa Ceweng Kecamatan Diwek Kabupaten Jombang. Subjek kasus pada ibu bersalin fisiologi multigravida. Hasil: Setelah dilakukan asuhan kebidanan pada ibu inpartu kala I fase aktif yang diberikan rangsangan puting susu selama 2 menit didapat bahwa kedua pasien mengalami penambahan intensitas kontraksi uterus. Dari 2 kali dalam 10 menit 30 detik menjadi 2 kali dalam 10 menit 50 detik. Ibu dapat bersalin dengan normal tanpa ada komplikasi. Keadaan ibu dan bayi baik. Pembahasan: Kesimpulan yang didapat dari kedua pasien bahwa stimulasi puting susu hanya meningkatkan intensitas kontraksi uterus tetapi tidak cukup kuat untuk meningkatkan frekuensi nya. Diharapkan untuk kehamilan selanjutnya pasien dapat menggunakan rangsangan puting susu sebagai salah satu upaya untuk meningkatkan kontraksi.   Kata Kunci: Persalinan, Rangsangan Puting Susu, Kontraksi &nbsp

Komunikasi Pendek Perbanyakan Tanaman Artemisia Annua Secara in Vitro

Artemisinin, an anti-malarial medicineisolated from the annual wormwood Artemisia annua,has a marked activity against chloroquine-resistant andchloroquine-sensitive strains of Plasmodium falciparum.This compound is useful for treatment of cerebral malaria.An in vitro propagation system for A. annua has been developed.Shoots were induced by culturing seeds of A.annua on a MS medium containing BAP (0, 0.1, 0.3, 0.5mg/l). Shoots were also formed on each seedling culturedon the same medium. Root formations were obtained fromshoots that were subcultured on a MS medium containingIBA (0, 1.0, 1.5, 2 mg/l). The results showed that MS mediumsupplemented with BAP 0.3 mg/l was the best medium forinduction and multiplication of the shoots, while the MSmedium supplemented with IBA (1 mg/l) was good for rootformations

The Implementation of Good Corporate Governance: Required Quality of Management Accounting Information System? (Empirical Evidence on Sharia Banking Institutions in Bandung City, Indonesia)

The aim of this study is to explain that the quality of management accounting information systems is able to influence the implementation of good corporate governance on sharia banking institutions in Bandung city, Indonesia. A contribution of thought to the Financial Services Authority (OJK) in enforcing regulations on the implementation of good governance on sharia banking institutions is the result from this study. The research method used was a data collection survey through questionnaires as the instrument of research to the respondents who are managers in sharia banking institutions. The population was 42 sharia banking institutions in Bandung city, Indonesia. The target of population was all 12 institutions of sharia commercial banks in Bandung city in 2017 period. All targets of population are taken as samples by using nonprobability sampling technique with convenience sampling method. And analysis of simple linear regression method is as the hypothesis testing. The quality of management accounting information system positively and significantly influences to the implementation of good corporate governance on sharia banking institutions in Bandung city, Indonesia, as it is shown by the results. In other words, it can be said that the better quality of management accounting information system is needed as it will make the better implementation of good corporate governance on sharia banking institutions in Bandung city, Indonesia as well.     Keywords: quality of management accounting information system, good corporate governance, sharia banking institutio

Uji Efektivitas Rebusan Belimbing Wuluh (Averrhoa Bilimbi) Sebagai Pengawet Alami Pada Ikan Teri Jengki (Stolephorus Heterolobus) Asin Kering

This study aims to look at the effect of the boiling water treatment by dosage variation star fruit and storage of dried salted anchovy dungarees from microbiological aspects. This research used experimental laboratories methods, using factorial RAL. The results showed that the higher the dosage of boiling water star fruit significantly affect the number of bacteria (

A quantitative assessment of distributions and sources of tropospheric halocarbons measured in Singapore.

This work reports the first ground-based atmospheric measurements of 26 halocarbons in Singapore, an urban-industrial city-state in Southeast (SE) Asia. A total of 166 whole air canister samples collected during two intensive 7 Southeast Asian Studies (7SEAS) campaigns (August-October 2011 and 2012) were analyzed for C1-C2 halocarbons using gas chromatography-electron capture/mass spectrometric detection. The halocarbon dataset was supplemented with measurements of selected non-methane hydrocarbons (NMHCs), C1-C5 alkyl nitrates, sulfur gases and carbon monoxide to better understand sources and atmospheric processes. The median observed atmospheric mixing ratios of CFCs, halons, CCl4 and CH3CCl3 were close to global tropospheric background levels, with enhancements in the 1-17% range. This provided the first measurement evidence from SE Asia of the effectiveness of Montreal Protocol and related national-scale regulations instituted in the 1990s to phase-out ozone depleting substances (ODS). First- and second-generation CFC replacements (HCFCs and HFCs) dominated the atmospheric halocarbon burden with HFC-134a, HCFC-22 and HCFC-141b exhibiting enhancements of 39-67%. By combining near-source measurements in Indonesia with receptor data in Singapore, regionally transported peat-forest burning smoke was found to impact levels of several NMHCs (ethane, ethyne, benzene, and propane) and short-lived halocarbons (CH3I, CH3Cl, and CH3Br) in a subset of the receptor samples. The strong signatures of these species near peat-forest fires were potentially affected by atmospheric dilution/mixing during transport and by mixing with substantial urban/regional backgrounds at the receptor. Quantitative source apportionment was carried out using positive matrix factorization (PMF), which identified industrial emissions related to refrigeration, foam blowing, and solvent use in chemical, pharmaceutical and electronics industries as the major source of halocarbons (34%) in Singapore. This was followed by marine and terrestrial biogenic activity (28%), residual levels of ODS from pre-Montreal Protocol operations (16%), seasonal incidences of peat-forest smoke (13%), and fumigation related to quarantine and pre-shipment (QPS) applications (7%)