Investigating long-term changes in polar stratospheric clouds above Antarctica: A temperature-based approach using spaceborne lidar detections

Polar stratospheric clouds play a significant role in the seasonal thinning of the ozone layer by facilitating the activation of stable chlorine and bromine reservoirs into reactive radicals, as well as prolonging the ozone depletion by removing HNO3 and H2O of the stratosphere by sedimentation. In a context of climate change, the cooling of the lower polar stratosphere could enhance the PSC formation and by consequence cause more ozone depletion. There is thus a need to document the evolution of the PSC cover to better understand its impact on the ozone layer. In this article we present a statistical model based on the analysis of the CALIPSO PSC product from 2006 to 2020. The model predicts the daily regionally-averaged PSC density by pressure level derived from stratospheric temperatures. Applying our model to stratospheric temperatures from the CALIPSO PSC product over the 2006–2020 period shows it is robust in the stratosphere between 10 and 150 hPa, reproducing well PSC variations over daily timescales and seasonal differences (2006–2020). The model reproduces well the PSC seasonal progression, even during disruptive events like stratospheric sudden warmings, except for years characterized by volcanic eruptions. We apply our model to gridded stratospheric temperatures from reanalyses over the complete south pole domain to evaluate changes in PSC seasons over the 1980–2021 period. We find two distinct periods in the evolution of the PSC season duration. Between 1980 and 2000, the PSC season increased by 15 days at 10–20 hPa with an increasing lengthening as we descend in altitudes to reach 30 days at 100–150 hPa. This lengthening is in possible relation with major eruptions occurring over this period. After 2000, a temporary drop mostly visible at high (10–20 hPa) and lower altitude (100–150 hPa) is followed by a progressive increase in PSC season duration. Over the 1980–2020 period, the PSC season increased by 20 days between 30–100 hPa. These changes are altitude-dependent and statistically significant. We discuss the impact of non-temperature stratospheric changes on the variations of PSC seasons

Detection of H1 swine influenza a virus antibodies in human serum samples by age group

Most H1 influenza A viruses (IAVs) of swine are derived from past human viruses. As human population immunity against these IAVs gradually decreases, the risk of reintroduction to humans increases. We examined 549 serum samples from persons 0-97 years of age collected in Belgium during 2017-2018 for hemagglutination inhibiting and virus neutralizing antibodies against 7 major H1 swine IAV (swIAV) clades and 3 human progenitor IAVs. Seroprevalence (titers >= 40) rates were >= 50% for classical swine and European human-like swIAVs, >= 24% for North American human-like delta 1a and Asian avian-like swIAVs, and <= 10% for North American human-like delta 1b and European avian-like swIAVs, but rates were age-dependent. Antibody titers against human-like swIAVs and supposed human precursor IAVs correlated with correlation coefficients of 0.30-0.86. Our serologic findings suggest that European avian-like, Glade 1C.2.1, and North American human-like delta 1b, Glade 16.2.2.2, H1 swIAVs pose the highest pandemic risk

Neutropenia as an adverse event following vaccination : results from randomized clinical trials in healthy adults and systematic review

Background : In the context of early vaccine trials aimed at evaluating the safety profile of novel vaccines, abnormal haematological values, such as neutropenia, are often reported. It is therefore important to evaluate how these trials should be planned not to miss potentially important safety signals, but also to understand the implications and the clinical relevance. Methodology : We report and discuss the results from five clinical trials (two with a new Shigella vaccine in the early stage of clinical development and three with licensed vaccines) where the absolute neutrophil counts (ANC) were evaluated before and after vaccination. Additionally, we have performed a systematic review of the literature on cases of neutropenia reported during vaccine trials to discuss our results in a more general context. Principal Findings : Both in our clinical trials and in the literature review, several cases of neutropenia have been reported, in the first two weeks after vaccination. However, neutropenia was generally transient and had a benign clinical outcome, after vaccination with either multiple novel candidates or well-known licensed vaccines. Additionally, the vaccine recipients with neutropenia frequently had lower baseline ANC than non-neutropenic vaccinees. In many instances neutropenia occurred in subjects of African descent, known to have lower ANC compared to western populations. Conclusions : It is important to include ANC and other haematological tests in early vaccine trials to identify potential safety signals. Post-vaccination neutropenia is not uncommon, generally transient and clinically benign, but many vaccine trials do not have a sampling schedule that allows its detection. Given ethnic variability in the level of circulating neutrophils, normal ranges taking into account ethnicity should be used for determination of trial inclusion/exclusion criteria and classification of neutropenia related adverse events

Validation of an enzyme-linked immunosorbent assay for the quantification of human IgG directed against the repeat region of the circumsporozoite protein of the parasite Plasmodium falciparum.

BACKGROUND: Several pre-erythrocytic malaria vaccines based on the circumsporozoite protein (CSP) antigen of Plasmodium falciparum are in clinical development. Vaccine immunogenicity is commonly evaluated by the determination of anti-CSP antibody levels using IgG-based assays, but no standard assay is available to allow comparison of the different vaccines. METHODS: The validation of an anti-CSP repeat region enzyme-linked immunosorbent assay (ELISA) is described. This assay is based on the binding of serum antibodies to R32LR, a recombinant protein composed of the repeat region of P. falciparum CSP. In addition to the original recombinant R32LR, an easy to purify recombinant His-tagged R32LR protein has been constructed to be used as solid phase antigen in the assay. Also, hybridoma cell lines have been generated producing human anti-R32LR monoclonal antibodies to be used as a potential inexhaustible source of anti-CSP repeats standard, instead of a reference serum. RESULTS: The anti-CSP repeats ELISA was shown to be robust, specific and linear within the analytical range, and adequately fulfilled all validation criteria as defined in the ICH guidelines. Furthermore, the coefficient of variation for repeatability and intermediate precision did not exceed 23%. Non-interference was demonstrated for R32LR-binding sera, and the assay was shown to be stable over time. CONCLUSIONS: This ELISA, specific for antibodies directed against the CSP repeat region, can be used as a standard assay for the determination of humoral immunogenicity in the development of any CSP-based P. falciparum malaria vaccine

Water-repellent and biocide treatments: Assessment of the potential combinations

International audienceIt is a common practice to use several chemical products during restoration projects of monuments or sculptures. However, care must be taken when combining the products to avoid a misuse. For example, it is well-known that applying a biocide on stone before a water-repellent leads to a diminishment of the hydrophobic effect of the treatment. But the application of biocide after a water-repellent treatment has been poorly analysed, although studies have proven that the stone looses its hydrophobicity after the application of the biocide. Henceforth, this study investigates the effects of biocide application on a water-repellent film and focuses on the possibilities to restore the efficiency of the previous water-repellent treatment (after the application of the biocide). At first, the tests were performed on glass slides to understand the mechanisms, with the subsequent results revealing that the biocide product deposits on the water-repellent film. Then, the study focuses on determining methods to remove the remains of biocide on limestone samples, previously treated with a water-repellent. The water-repellent used in the study is an alkylpolysiloxane, Rhodorsil H224 from Rhodia

Impact of Complex-Logic Cell Layout on the Single-Event Transient Sensitivity

International audienceThe design methodology based on standard cells is widely used in a broad range of VLSI applications. Further, several optimization algorithms can be employed to address different constraints such as power consumption or reliability. This work evaluates the implications of the usage of complex-logic cells from a 45 nm Standard-Cell library to the Single-Event Transient sensitivity under heavy ions. Results show that even though a reduction in the layout area is obtained when adopting complex-logic gates, a slight reduction in the total sensitive area of the circuit is observed. Moreover, the effectiveness of logical masking can be suppressed, leading to a higher SET cross-section

Lung progenitors from lambs can differentiate into specialized alveolar or bronchiolar epithelial cells.

26International audienceBACKGROUND: Airways progenitors may be involved in embryogenesis and lung repair. The characterization of these important populations may enable development of new therapeutics to treat acute or chronic lung disease. In this study, we aimed to establish the presence of bronchioloalveolar progenitors in ovine lungs and to characterize their potential to differentiate into specialized cells. RESULTS: Lung cells were studied using immunohistochemistry on frozen sections of the lung. Immunocytochemistry and flow cytometry were conducted on ex-vivo derived pulmonary cells. The bronchioloalveolar progenitors were identified by their co-expression of CCSP, SP-C and CD34. A minor population of CD34pos/SP-Cpos/CCSPpos cells (0.33% +/- 0.31) was present ex vivo in cell suspensions from dissociated lungs. Using CD34 magnetic positive-cell sorting, undifferentiated SP-Cpos/CCSPpos cells were purified (>80%) and maintained in culture. Using synthetic media and various extracellular matrices, SP-Cpos/CCSPpos cells differentiated into either club cells (formerly named Clara cells) or alveolar epithelial type-II cells. Furthermore, these ex vivo and in vitro derived bronchioloalveolar progenitors expressed NANOG, OCT4 and BMI1, specifically described in progenitors or stem cells, and during lung development. CONCLUSIONS: We report for the first time in a large animal the existence of bronchioloalveolar progenitors with dual differentiation potential and the expression of specialized genes. These newly described cell population in sheep could be implicated in regeneration of the lung following lesions or in development of diseases such as cancers

Spectral Phase Control of Interfering Chirped Pulses for High-Energy Narrowband Terahertz Generation

Highly-efficient optical generation of narrowband terahertz (THz) radiation enables unexplored technologies and sciences from compact electron acceleration to charge manipulation in solids. State-of-the-art conversion efficiencies are currently achieved using difference-frequency generation (DFG) driven by temporal beating of chirped pulses but remain, however, far lower than desired or predicted. Here we show that high-order spectral phase fundamentally limits the efficiency of narrowband DFG using chirped-pulse beating and resolve this limitation by introducing a novel technique based on tuning the relative spectral phase of the pulses. For optical terahertz generation, we demonstrate a 13-fold enhancement in conversion efficiency for 1%-bandwidth, 0.361 THz pulses, yielding a record energy of 0.6 mJ and exceeding previous optically-generated energies by over an order of magnitude. Our results prove the feasibility of millijoule-scale applications like terahertz-based electron accelerators and light sources and solve the long-standing problem of temporal irregularities in the pulse trains generated by interfering chirped pulses.Comment: 25 pages, 5 figures, updated to the state before review at Nature Communications (updated the affiliations, title, some content, methods, etc.