112 research outputs found
Hall, Seebeck, and Nernst Coefficients of Underdoped HgBa2CuO4+d: Fermi-Surface Reconstruction in an Archetypal Cuprate Superconductor
Charge density-wave order has been observed in cuprate superconductors whose
crystal structure breaks the square symmetry of the CuO2 planes, such as
orthorhombic YBa2Cu3Oy (YBCO), but not so far in cuprates that preserve that
symmetry, such as tetragonal HgBa2CuO4+d (Hg1201). We have measured the Hall
(R_H), Seebeck (S), and Nernst coefficients of underdoped Hg1201 in magnetic
fields large enough to suppress superconductivity. The high-field R_H(T) and
S(T) are found to drop with decreasing temperature and become negative, as also
observed in YBCO at comparable doping. In YBCO, the negative R_H and S are
signatures of a small electron pocket caused by Fermi-surface reconstruction,
attributed to charge density-wave modulations observed in the same range of
doping and temperature. We deduce that a similar Fermi-surface reconstruction
takes place in Hg1201, evidence that density-wave order exists in this
material. A striking similarity is also found in the normal-state Nernst
coefficient, further supporting this interpretation. Given the model nature of
Hg1201, Fermi-surface reconstruction appears to be common to all hole-doped
cuprates, suggesting that density-wave order is a fundamental property of these
materials
Semiflexible chains in confined spaces
We develop an analytical method for studying the properties of a noninteracting wormlike chain (WLC) in confined geometries. The mean-field-like theory replaces the rigid constraints of confinement with average constraints, thus allowing us to develop a tractable method for treating a WLC wrapped on the surface of a sphere, and fully encapsulated within it. The efficacy of the theory is established by reproducing the exact correlation functions for a WLC confined to the surface of a sphere. In addition, the coefficients in the free energy are exactly calculated. We also describe the behavior of a surface-confined chain under external tension that is relevant for single molecule experiments on histone-DNA complexes. The force-extension curves display spatial oscillations, and the extension of the chain, whose maximum value is bounded by the sphere diameter, scales as f−1 at large forces, in contrast to the unconfined chain that approaches the contour length as f−1∕2. A WLC encapsulated in a sphere, that is relevant for the study of the viral encapsulation of DNA, can also be treated using the mean-field approach. The predictions of the theory for various correlation functions are in excellent agreement with Langevin simulations. We find that strongly confined chains are highly structured by examining the correlations using a local winding axis. The predicted pressure of the system is in excellent agreement with simulations but, as is known, is significantly lower than the pressures seen for DNA packaged in viral capsids
Evidence for a small hole pocket in the Fermi surface of underdoped YBa2Cu3Oy
The Fermi surface of a metal is the fundamental basis from which its
properties can be understood. In underdoped cuprate superconductors, the Fermi
surface undergoes a reconstruction that produces a small electron pocket, but
whether there is another, as yet undetected portion to the Fermi surface is
unknown. Establishing the complete topology of the Fermi surface is key to
identifying the mechanism responsible for its reconstruction. Here we report
the discovery of a second Fermi pocket in underdoped YBa2Cu3Oy, detected as a
small quantum oscillation frequency in the thermoelectric response and in the
c-axis resistance. The field-angle dependence of the frequency demonstrates
that it is a distinct Fermi surface and the normal-state thermopower requires
it to be a hole pocket. A Fermi surface consisting of one electron pocket and
two hole pockets with the measured areas and masses is consistent with a
Fermi-surface reconstruction caused by the charge-density-wave order observed
in YBa2Cu3Oy, provided other parts of the reconstructed Fermi surface are
removed by a separate mechanism, possibly the pseudogap.Comment: 23 pages, 5 figure
Fermi Surface of the Electron-doped Cuprate Superconductor Nd_{2-x}Ce_xCuO_{4} Probed by High-Field Magnetotransport
We report on the study of the Fermi surface of the electron-doped cuprate
superconductor NdCeCuO by measuring the interlayer
magnetoresistance as a function of the strength and orientation of the applied
magnetic field. We performed experiments in both steady and pulsed magnetic
fields on high-quality single crystals with Ce concentrations of to
0.17. In the overdoped regime of we found both semiclassical
angle-dependent magnetoresistance oscillations (AMRO) and Shubnikov-de Haas
(SdH) oscillations. The combined AMRO and SdH data clearly show that the
appearance of fast SdH oscillations in strongly overdoped samples is caused by
magnetic breakdown. This observation provides clear evidence for a
reconstructed multiply-connected Fermi surface up to the very end of the
overdoped regime at . The strength of the superlattice potential
responsible for the reconstructed Fermi surface is found to decrease with
increasing doping level and likely vanishes at the same carrier concentration
as superconductivity, suggesting a close relation between translational
symmetry breaking and superconducting pairing. A detailed analysis of the
high-resolution SdH data allowed us to determine the effective cyclotron mass
and Dingle temperature, as well as to estimate the magnetic breakdown field in
the overdoped regime.Comment: 23 pages, 8 figure
Cryo-electron microscopy of viruses
Thin vitrified layers of unfixed, unstained and unsupported virus suspensions can be prepared for observation by cryo-electron microscopy in easily controlled conditions. The viral particles appear free from the kind of damage caused by dehydration, freezing or adsorption to a support that is encountered in preparing biological samples for conventional electron microscopy. Cryo-electron microscopy of vitrified specimens offers possibilities for high resolution observations that compare favourably with any other electron microscopical method
On the Effect of Thermodynamic Equilibrium on the Assembly Efficiency of Complex Multi-Layered Virus-Like Particles (VLP): the Case of Rotavirus VLP
Previous studies have reported the production of malformed virus-like-particles (VLP) in recombinant host systems. Here we computationally investigate the case of a large triple-layered rotavirus VLP (RLP). In vitro assembly, disassembly and reassembly data provides strong evidence of microscopic reversibility of RLP assembly. Light scattering experimental data also evidences a slow and reversible assembly untypical of kinetic traps, thus further strengthening the fidelity of a thermodynamically controlled assembly. In silico analysis further reveals that under favourable conditions particles distribution is dominated by structural subunits and completely built icosahedra, while other intermediates are present only at residual concentrations. Except for harshly unfavourable conditions, assembly yield is maximised when proteins are provided in the same VLP protein mass composition. The assembly yield decreases abruptly due to thermodynamic equilibrium when the VLP protein mass composition is not obeyed. The latter effect is more pronounced the higher the Gibbs free energy of subunit association is and the more complex the particle is. Overall this study shows that the correct formation of complex multi-layered VLPs is restricted to a narrow range of association energies and protein concentrations, thus the choice of the host system is critical for successful assembly. Likewise, the dynamic control of intracellular protein expression rates becomes very important to minimize wasted proteins
High Distribution of CD40 and TRAF2 in Th40 T Cell Rafts Leads to Preferential Survival of this Auto-Aggressive Population in Autoimmunity
CD40-CD154 interactions have proven critical in autoimmunity, with the identification of CD4(lo)CD40(+) T cells (Th40 cells) as harboring an autoaggressive T cell population shedding new insights into those disease processes. Th40 cells are present at contained levels in non-autoimmune individuals but are significantly expanded in autoimmunity. Th40 cells are necessary and sufficient in transferring type 1 diabetes in mouse models. However, little is known about CD40 signaling in T cells and whether there are differences in that signaling and subsequent outcome depending on disease conditions. When CD40 is engaged, CD40 and TNF-receptor associated factors, TRAFs, become associated with lipid raft microdomains. Dysregulation of T cell homeostasis is emerging as a major contributor to autoimmune disease and thwarted apoptosis is key in breaking homeostasis.Cells were sorted into CD4(hi) and CD4(lo) (Th40 cells) then treated and assayed either as whole or fractionated cell lysates. Protein expression was assayed by western blot and Nf-kappaB DNA-binding activity by electrophoretic mobility shifts. We demonstrate here that autoimmune NOD Th40 cells have drastically exaggerated expression of CD40 on a per-cell-basis compared to non-autoimmune BALB/c. Immediately ex-vivo, untreated Th40 cells from NOD mice have high levels of CD40 and TRAF2 associated with the raft microdomain while Th40 cells from NOR and BALB/c mice do not. CD40 engagement of Th40 cells induces Nf-kappaB DNA-binding activity and anti-apoptotic Bcl-X(L) expression in all three mouse strains. However, only in NOD Th40 cells is anti-apoptotic cFLIP(p43) induced which leads to preferential survival and proliferation. Importantly, CD40 engagement rescues NOD Th40 cells from Fas-induced death.CD40 may act as a switch between life and death promoting signals and NOD Th40 cells are poised for survival via this switch. This may explain how they expand in autoimmunity to thwart T cell homeostasis
Protection against live rotavirus challenge in mice induced by parenteral and mucosal delivery of VP6 subunit rotavirus vaccine
Live oral rotavirus (RV) vaccines are part of
routine childhood immunization but are associated with
adverse effects, particularly intussusception. We have developed
a non-live combined RV – norovirus (NoV) vaccine
candidate consisting of human RV inner-capsid rVP6
protein and NoV virus-like particles. To determine the
effect of delivery route on induction of VP6-specific protective
immunity, BALB/c mice were administered a vaccine
containing RV rVP6 intramuscularly, intranasally or a
combination of both, and challenged with murine RV. At
least 65 % protection against RV shedding was observed
regardless of delivery route. The levels of post-challenge
serum VP6-specific IgA titers correlated with protection
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