Preliminary study on density-dependent flow and transport in the Lake Karachai area (UE RaCoS project): adequacy of CODESA-3D to model a plume of contaminant brine

One of the main objectives in this preliminary study was to implement CODESA-3D (COupled variable DEnsity and SAturation) code for the Lake Karachai site and to run preliminary simulations under a variety of conditions, but with density ratio coeffcient no larger than 0.07 (density variations of maximum 7%). Because CODESA-3D is a finite element numerical code for coupled flow and transport that is normally used for problems of seawater intrusion, where density variations are around 3%, part of our work has been devoted to determine the limit of applicability of the code

Diving lice: The exception to the rule that there are no insects in the (deep) ocean

Insects are present in virtually all possible habitats on the mainland but virtually absent in the ocean which constitutes 90% of the biosphere. Only a few species are present in the sea, but remaining at the surface, salt marshes, estuaries, or shallow waters. Remarkably, a group of species manages to endure long immersion periods in the open sea, as well as deep dives, i.e. seal lice. We discuss here some of the adaptations that allow seal lice to cope with the amphibious habits of their hosts and how they can help understanding why are insect so rare in the ocean.Fil: Leonardi, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; ArgentinaFil: Crespo, José Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; ArgentinaFil: Soto, Florencia Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Instituto de Biología de Organismos Marinos; ArgentinaFil: Lazzari, Claudio R.. Universite de Tours; Francia. Centre National de la Recherche Scientifique; FranciaThe 1st International Electronic Conference on EntomologySuizaInsects JournalMultidisciplinary Digital Publishing Institut

Specific Inhibition of the Redox Activity of Ape1/Ref-1 by E3330 Blocks Tnf-A-Induced Activation of Il-8 Production in Liver Cancer Cell Lines

APE1/Ref-1 is a main regulator of cellular response to oxidative stress via DNA-repair function and co-activating activity on the NF-κB transcription factor. APE1 is central in controlling the oxidative stress-based inflammatory processes through modulation of cytokines expression and its overexpression is responsible for the onset of chemoresistance in different tumors including hepatic cancer. We examined the functional role of APE1 overexpression during hepatic cell damage related to fatty acid accumulation and the role of the redox function of APE1 in the inflammatory process. HepG2 cells were stably transfected with functional and non-functional APE1 encoding plasmids and the protective effect of APE1 overexpression toward genotoxic compounds or FAs accumulation, was tested. JHH6 cells were stimulated with TNF-α in the presence or absence of E3330, an APE1 redox inhibitor. IL-8 promoter activity was assessed by a luciferase reporter assay, gene expression by Real-Time PCR and cytokines (IL-6, IL-8, IL-12) levels measured by ELISA. APE1 over-expression did not prevent cytotoxicity induced by lipid accumulation. E3330 treatment prevented the functional activation of NF-κB via the alteration of APE1 subcellular trafficking and reduced IL-6 and IL-8 expression induced by TNF-α and FAs accumulation through blockage of the redox-mediated activation of NF-κB. APE1 overexpression observed in hepatic cancer cells may reflect an adaptive response to cell damage and may be responsible for further cell resistance to chemotherapy and for the onset of inflammatory response. The efficacy of the inhibition of APE1 redox activity in blocking TNF-α and FAs induced inflammatory response opens new perspectives for treatment of inflammatory-based liver diseases

Clip-Air, a flexible air transportation system

We introduce a concept of flexible air transportation system called Clip-Air. It is a modular innovative aircraft. The flexibility provided by Clip-Air is due to the decoupling of load and carrying units. In this paper, we introduce the concept, and analyze the impacts from the airlines perspective. An integrated schedule design and fleet assignment model is developed for both standard airline fleets and Clip-Air. The model considers spill and recapture effects to represent the demand in case of capacity shortage. Recapture ratios between available itineraries in each market segment are appropriately calculated through an itinerary choice model. The comparative analysis is carried out under different scenarios which are selected with the purpose of understanding the effects of the network structure, fleet size, fleet configuration and the estimated cost figures for the Clip-Air system. It is observed that Clip-Air is able to carry on the average 5-10 % more passengers by using 20-30 % less overall capacity. Moreover, Clip-Air is found to deal better with insufficient transportation capacity. Furthermore, the scheduling decisions are robust to the estimated cost figures of Clip-Air. For the analyzed range of costs Clip-Air is always carrying more passengers with less allocated capacity compared to standard fleet

Systems engineering and design of a Mars Polar Research Base with a human crew

Mars Polar Ice caps have been known ever since they were first observed by Cassini. Robotic exploration mis- sions, starting with Mariner 9, have confirmed that they are composed of water ice. During later missions, instruments such as Mars Global Surveyor's MOLA have established a detailed topography and have estimated their depth at about 3 km in the thickest part, while detailed internal structure has been investigated by MARSIS from Mars Express and SHARAD from the Mars Reconnaissance Orbiter. This analysis proposes to establish a base near North Polar Layered Deposits to investigate Mars' climate, hydrological processes and to test for possible traces of life. The objectives of the mission are to sustain a crew for nine months on the surface of Mars, near the North Pole, and to bring the crew back to Earth safely. During the surface mission, the crew will drill and analyze Polar Layered Deposits in ice samples. Furthermore, because the North Polar region provides an easy access to water ice, this area has the potential of sustaining a long-term human presence. The Mars Polar Research mission shall therefore prepare for long term missions, spanning over multiple crew generations. Indeed, longer duration missions and larger crews should be facilitated by this first mission. This paper describes a mission design for a Mars Polar Research base using systems engineering approach and scenario testing. The goal of the work is to establish a strategy composed of various technologies that have been selected accordingly. The requirements related to crew composition, human physiology and psychology adaptation, quality of com- munication, challenges and prospects of advancing science, as well as optimum habitat design and its usability, are derived and compiled into mass, volume, data and power consumption. A design for the base and mission scenario is also proposed. Given the identified requirements, possible technologies for life support systems, radiation protection, in-situ propellant production, thermal control, air pressure difference compensation and availability of power are discussed and solutions to focus on are recommended. Furthermore, the requirements for a long-term mission preparation are also identified and solutions to include in a first Mars mission with crew are recommended. In conclusion, approximately 110 metric tons and 160 kW are required to enable a Mars Polar mission with a human crew. A two-phase mission is recommended for enabling the testing of key in-situ resource utilization technologies allowing to minimize mass, while ensuring the security of the crew. The use of optimal payload and fairing, a Mars orbit crane system and deployable structures are recommended. Finally, in pre- paration for a long-term presence of humans on Mars, including in-situ testing of key technologies enabling the production of consumables facilitating autonomy from Earth is suggested. The consumables that have been identified as not being able to be tested before a first crew is sent to Mars are food and energy production. These developments may serve as priorities for current Mars settlement programs

Effect of drug incorporation technique and polymer combination on the performance of biopolymeric antifungal buccal films

Numerous films with a dissolved or dispersed active principle within a polymeric matrix have been described in literature. However, the incorporation of solid crystals into the films may influence several relevant properties. Additionally, it has been reported that different polymeric matrices lead to films presenting a different performance. The aim of this work was to evaluate the effect of the combination of chitosan with carrageenan (κ-, λ-, and ι-) as matrices, and of the miconazole nitrate incorporation method, on the films behavior. Mechanical properties, drug release and antifungal activity were evaluated. The state of the drug in the films was analyzed by different techniques. Films showed a homogeneous surface and a thermal protective effect on the drug. The combination of chitosan and λ-carrageenan leads to films with the highest values of tensile and mucoadhesive strength. Films with solubilized drug displayed slightly higher elongation at break, tensile and mucoadhesive strength and faster drug release than those with suspended miconazole nitrate. However, no differences were found regarding the antifungal activity of the different formulations including time-to-kill curves.Fil: Tejada Jacob, Guillermo Ivan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Lamas, Maria Celina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Svetaz, Laura Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; ArgentinaFil: Salomon, Claudio Javier. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alvarez, Vera Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leonardi, Darío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentin

Recent advances in the treatment of Chagas disease

Descoberta há cem anos, a doença de Chagas afeta a mais de quinze milhões de pessoas em toda a América Latina e, ainda hoje, não há tratamentoeficaz. O fármaco benznidazol, utilizado como única opção de tratamento no Brasil, é ineficaz na fase crônica da doença. Problemas relacionados à biodisponibilidade do medicamento comercial limitam sua eficácia, principalmente na fase crônica, quando os parasitos estão confinados em tecidos profundos e em lenta replicação. Nesse contexto, pesquisas lideradas por grupos brasileiros e argentinos vêm sendo conduzidas com o objetivo de desenvolver formulações de benznidazol mais eficientes. Diversas formas farmacêuticas sólidas e líquidas foram propostas nos últimos anos com resultados pré-clínicos promissores, sendo descritas melhorias acentuadas nas características farmacocinéticas desse fármaco. Espera-se que as formas inovadoras apresentadas possam ser avaliadas em ensaios clínicos e incorporadas à produção industrial em breve.Discovered about a hundred years ago, Chagas disease currently affects more than fifteen million people in Latin America, and it still remains without any effective treatment. Although benznidazole has been used as the only pharmacotherapeutic option to treat Chagas disease in Brazil, it is ineffective in the chronic phase of the disease, when the parasites are confined to deep tissue layers and slowly replicate. This happens mainly due to problems related to the bioavailability of the drug, which is currently in the market. In this context, Brazilian and Argentinean research groups have conducted studies to develop more efficient benznidazole formulations. Several solid and liquid formulations have been proposed over the last few years with promising preclinical results. Improvements in the pharmacokinetic properties of this drug have been described. Therefore, it is expected, that such innovative drugs and formulations be assessed in clinical trials and soon incorporated to industrial production.Fil: da Cunha Filho, Marcílio Sérgio Soares . Universidade Do Brasilia; BrasilFil: de Sá-Barreto, Lívia Cristina lira . Universidade Do Brasilia; BrasilFil: Leonardi, Darío. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; ArgentinaFil: Lamas, Maria Celina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; ArgentinaFil: Salomon, Claudio Javier. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Química Rosario; Argentin

Exploiting the Reducing Properties of Lignin for the Development of an Effective Lignin@Cu2O Pesticide

Lignin is a natural polymer produced in huge amounts by the paper industry. Innovative applications of lignin, especially in agriculture, represent a valuable way to develop a more sustainable economy. Its antioxidant and antimicrobial properties, combined with its biodegradability, make it particularly attractive for the development of plant protection products. Copper is an element that has long been used as a pesticide in agriculture. Despite its recognized antimicrobial activity, the concerns derived from its negative environmental impact is forcing research to move toward the development of more effective and sustainable copper-based pesticides. Here a simple and sustainable way of synthesizing a new hybrid material composed of Cu2O nanocrystals embedded into lignin, named Lignin@Cu2O is presented. The formation of cuprite nanocrystals leaves the biopolymer intact, as evidenced by infrared spectroscopy, gel permeation chromatography, and Pyrolysis-GC analysis. The combined activity of lignin and cuprite make Lignin@Cu2O effective against Listeria monocytogenes and Rhizoctonia solani at low copper dosage, as evidenced by in vitro and in vivo tests conducted on tomato plants