Selective and “Veiled” Demarketing from the Perspective of Black Female Consumers

This study sheds light on the perspective of Black female consumers in regards to certain effects of marketing initiatives adopting the literature on demarketing as a framework. The context examined is their experience with the market of hair beauty and care. Media actions are analyzed along narrative interviews in order to understand the phenomenon. The findings reveal a structural dominant pattern which perpetuates the marginalized status of Black women’s natural traits. Emerging market initiatives point to movements concerning the visibility of such female consumers, who despite being eager to consume, have their demand discouraged. This suggests that they perceive a selective and veiled demarketing, as one of the results of marketing actions. The analysis invites for theoretical reflections on demarketing and veiled racism in Brazil

The Web Experiment List: A Web service for the recruitment of participants and archiving of Internet-based experiments

The Web Experiment List (http://genpsylabwexlist.unizh.ch/), a free Web-based service for the recruitment of participants in Internet-based experiments, is presented. The Web Experiment List also serves as a searchable archive for the research community. It lists more than 250 links to and descriptions of current and past Web experiments. Searches can be conducted by area of research, language, type of study, date, and status (active vs. archived). Data from log file analyses reveal an increasing use of the Web Experiment List and provide a picture of the distribution of the use of the Web experiment method across disciplines. On a general theoretical note, Web services are discussed as a viable software alternative to the traditional program format

Optimisation of the foot-and-mouth disease virus 2A co-expression system for biomedical applications

This work was supported by the Medical Research Council.Background Many biomedical applications require the expression or production of therapeutic hetero-multimeric proteins/protein complexes: in most cases only accomplished by co-ordinated co-expression within the same cell. Foot-and-mouth disease virus 2A (F2A) and ‘2A-like’ sequences are now widely used for this purpose. Since 2A mediates a co-translational ‘cleavage’ at its own C-terminus, sequences encoding multiple proteins (linked via 2As) can be concatenated into a single ORF: a single transgene. It has been shown that in some cases, however, the cleavage efficiency of shorter versions of F2A may be inhibited by the C-terminus of certain gene sequences immediately upstream of F2A. This paper describes further work to optimise F2A for co-expression strategies. Results We have inserted F2A of various lengths in between GFP and CherryFP ‘reporter’ proteins (in reciprocal or tandem arrangements). The co-expression of these proteins and cleavage efficiencies of F2As of various lengths were studied by in vitro coupled transcription and translation in rabbit reticulocyte lysates, western blotting of HeLa cell lysates and fluorescence microscopy. Conclusions Optimal and suboptimal lengths of F2A sequences were identified as a result of detailed ‘fine-tuning’ of the F2A sequence. Based on our data and the model according to which 2A activity is a product of its interaction with the exit tunnel of the ribosome, we suggest the length of the F2A sequence which is not ‘sensitive’ to the C-terminus of the upstream protein that can be successfully used for co-expression of two proteins for biomedical applications.Publisher PDFPeer reviewe