Influence of dietary Chlorella vulgaris and carbohydrate-active enzymes on growth performance, meat quality and lipid composition of broiler chickens

Article in pressHerein, we investigated the effect of Chlorella vulgaris as ingredient (10% of incorporation) in broiler diets, supplemented or not with 2 formulations of Carbohydrate-Active enZymes (CAZymes; Rovabio Excel AP and a mixture of recombinant CAZymes, composed by an exo-b-glucosaminidase, an alginate lyase, a peptidoglycan N-acetylmuramic acid deacetylase and a lysozyme), on growth performance, meat quality, fatty acid composition, oxidative stability, and sensory traits. One hundred twenty 1-day-old Ross 308 male birds were randomly assigned to one of the 4 experimental diets (n 5 30): corn-soybean meal–basal diet (control), basal diet with 10% C. vulgaris (CV), CV supplemented with 0.005% of a commercialCAZymecocktail (Rovabio Excel AP), (CV1R), and CV supplemented with 0.01% of a 4- CAZyme mixture previously selected (CV 1 M) during the experimental period lasted from day 21 to day 35. Body weight gain and feed conversion rate of broilers were not affected by C. vulgaris but digesta viscosity increased more than 2-fold (P , 0.001) relative to the control. In addition, neither cooking loss, shear force, juiciness, flavor nor off-flavor was impaired by dietary treatments (P.0.05). By contrast, the dietary C. vulgaris increased tenderness, yellowness (b*) and total carotenoids in breast and thigh meats. However, no additional protective effect against lipid oxidation was observed in meat with the inclusion of microalga. Chlorella vulgaris, independently of CAZymes, had a minor impact on meat fatty acid composition but improved the proportion of some beneficial fatty acids. In summary, our data indicate a slight improvement of broiler meat quality and lipid nutritional value, without impairment of broilers’ growth performance, thus supporting the usefulness of this microalga in poultry diets, up to this high level of incorporation. By contrast, the selected CAZyme mixtures used do not significantly improve the release of microalga nutrients in poultry diets, through the disruption of microalga cell wall, which warrants further researchinfo:eu-repo/semantics/acceptedVersio

Farinha de mandioca enriquecida com bioproteínas (Saccharomyces cerevisiae), em associação ao feijão e arroz, na dieta de ratos em crescimento Cassava flour enriched with yeast (Saccharomyces cerevisiae) protein, in association with beans and rice, in the diet of growing rats

Avaliou-se o efeito da mistura de feijão, arroz e farinha de mandioca enriquecida com bioproteína (Saccharomyces cerevisiae), em ratos wistar machos recém-desmamados (n=60), durante 28 dias. Foram utilizadas as seguintes dietas: experimentais (feijão, arroz e farinha de mandioca enriquecida com leveduras; feijão, arroz e farinha de mandioca comum); controle (farinha de mandioca enriquecida com levedura); e padrão (caseína). Determinaram-se os testes biológicos. Os orgãos foram removidos para análise de pesos úmido e seco (rim esquerdo, baço e amostras do fígado e cérebro), teor de proteína (fígado e cérebro) e histopatologia (fígado, coração e rim direito). Foram ainda quantificados os lipídios totais da carcaça dos animais. Os dados foram estatisticamente avaliados pelo teste Não Paramétrico de Kruskal-Wallis e pelo teste de Comparações Múltiplas (p<0,05). Em todos os parâmetros foram evidenciados melhores resultados com a dieta padrão, seguida das experimentais. Conclui-se que a farinha de mandioca enriquecida com bioproteína poderá ser usada como complemento alimentar para humanos, visando combater as deficiências nutricionais de segmentos populacionais carentes.<br>The effect of a mixture of beans, rice and cassava flour enriched with yeast (Saccharomyces cerevisiae) protein was assessed in weanling male Wistar rats (n=60), during 28 days. The following diets were used: experimental (beans, rice and manioc flour with yeast protein; beans, rice and cassava flour without yeast protein); control (cassava flour with yeast protein); and standard (casein). The biological test were determined. The organs were removed for evaluation of wet and dry weights (left kidney, spleen and liver and brain samples), protein levels (liver and brain), and histopathology (heart, right kidney and liver). Carcass total lipids were also recorded. Results were statistically analyzed by the Nonparametric Test of Kruskal-Wallis and the Test of Multiple Comparisons (p<0.05). The highest values for all investigated parameters were found in the casein-fed group, followed by the experimental groups. Data suggest that flour enriched with yeast protein can be recommended as a dietary supplement to eradicate the nutritional deficiency in the poor population