Evaluating the Progress of the Liberalization of International Aviation toward Open Skies

The United States has engaged in well over 100 Open Skies Agreements with other ICAO member state partners reaching all parts of the globe. These Open Skies Agreements have established a practice of liberalization for airlines to have the most freedom to choose when, where, how often, and for how much they fly to locations. Despite a majority of ICAO member state partners engaging in Open Skies, there has been a reluctance of the member states to engage in the same practices with other aviation partners for similar access. A similar pattern is also evident for liberalization through the Freedoms of the Air, a key philosophical understanding set forth through ICAO practices describing the ways in which airlines can fly between the member states in the interest of international aviation. This paper evaluates the trend among the member states to engage in more liberalized aviation through their granted access to reduced government oversight of foreign airline access to sovereign airspace and the number of rights granted to their respective operational international partners. While the overwhelming number of agreements may not be fully liberalized Open Skies, there does appear to be an increasing desire to promote practices that connect member states at greater efficiencies and give travelers more options and more access to airline choice

Implementing Jet Aircraft Training in a University Setting: Instructor Perceptions and Lessons Learned

This paper presents the findings of a phenomenological study of instructor pilot first-hand experiences when conducting training for collegiate flight students in a jet aircraft. While jet training has been conducted in simulators in the past, this was one of the first instances of training in an actual aircraft. A total of 22 students completed training in a very light jet aircraft during the spring semester of their junior year at the subject university. A group of four instructors conducted both simulator and flight training with the students. Surveys were used to collect data from instructors longitudinally throughout the length of the 16-week semester. At the conclusion of the training period, participants completed a structured interview. The results of those interviews suggested that students excelled in areas such as avionics programming, use of standardized operating procedures, and checklist usage. Students were challenged by the increased operating speeds of the jet aircraft, descent planning, and lesson preparedness. The instructors offered suggestions to enhance the course and provided a summary of the lessons learned

ICAO safety management systems (SMS) development in environmental contexts: A field study of greater China

This was a mixed-methods exploratory study to investigate association between environmental context and the implementation status of Safety Management Systems (SMS) at airports in Greater China. Using a framework of Institutional Theory, this study looked at three regions of Greater China and explored internal and external environments of SMS at airports within each region. It used ICAO standards to evaluate the implementation status of SMS at those airports based on the perceptions of 126 participants. This research also employed snowballing technique to spread a survey tool to participants in Greater China through several key gatekeepers, and then applied the Delphi method for interviews with key gatekeepers themselves. Analysis of the data suggested several associations between various sub-concepts of the external environment and different components of SMS in the three regions. In addition, the data suggested a relationship between the internal environment as a whole and the overall status of SMS implementation in each region. Lastly, the study makes several recommendations for future research regarding global standards implemented in local environments, the evaluation of SMS implementation status, and the theoretical implications of this study

Evaluating the Progress of the Liberalization of International Aviation toward Open Skies

The United States has engaged in well over 100 Open Skies Agreements with other ICAO member state partners reaching all parts of the globe. These Open Skies Agreements have established a practice of liberalization for airlines to have the most freedom to choose when, where, how often, and for how much they fly to locations. Despite a majority of ICAO member state partners engaging in Open Skies, there has been a reluctance of the member states to engage in the same practices with other aviation partners for similar access. A similar pattern is also evident for liberalization through the Freedoms of the Air, a key philosophical understanding set forth through ICAO practices describing the ways in which airlines can fly between the member states in the interest of international aviation. This paper evaluates the trend among the member states to engage in more liberalized aviation through their granted access to reduced government oversight of foreign airline access to sovereign airspace and the number of rights granted to their respective operational international partners. While the overwhelming number of agreements may not be fully liberalized Open Skies, there does appear to be an increasing desire to promote practices that connect member states at greater efficiencies and give travelers more options and more access to airline choice

Implementing Jet Aircraft Training in a University Setting: Instructor Perceptions and Lessons Learned

This paper presents the findings of a phenomenological study of instructor pilot first-hand experiences when conducting training for collegiate flight students in a jet aircraft. While jet training has been conducted in simulators in the past, this was one of the first instances of training in an actual aircraft. A total of 22 students completed training in a very light jet aircraft during the spring semester of their junior year at the subject university. A group of four instructors conducted both simulator and flight training with the students. Surveys were used tocollect data from instructors longitudinally throughout the length of the 16-week semester. At the conclusion of the training period, participants completed a structured interview. The results of those interviews suggested that students excelled in areas such as avionics programming, use of standardized operating procedures, and checklist usage. Students were challenged by the increased operating speeds of the jet aircraft, descent planning, and lesson preparedness. The instructors offered suggestions to enhance the course and provided a summary of the lessons learned

Major Histocompatibility Complex-Restricted CD8\u3csup\u3e+\u3c/sup\u3e Cytotoxic T Lymphocytes from Horses with Equine Infectious Anemia Virus Recognize Env and Gag/PR Proteins

Cytotoxic T lymphocytes (CTL) can control some viral infections and may be important in the control of lentiviruses, including human immunodeficiency virus type 1. Since there is limited evidence for an in vivo role of CTL in control of lentiviruses, dissection of immune mechanisms in animal lentiviral infections may provide needed information. Horses infected with equine infectious anemia virus (EIAV), a lentivirus, have acute plasma viremia which is terminated in immunocompetent horses. Viremic episodes may recur, but most horses ultimately control infection and become asymptomatic carriers. To begin dissection of the immune mechanisms involved in EIAV control, peripheral blood mononuclear cells (PBMC) from infected horses were evaluated for CTL to EIAV-infected cells. By using noninfected and EIAV-infected autologous equine kidney (EK) cells in Cr-release assays, EIAV-specific cytotoxic activity was detected in unstimulated PBMC from three infected horses. The EIAV-specific cytotoxic activity was major histocompatibility complex (MHC) restricted, as determined by assaying EIAV-infected heterologous EK targets, and was mediated by CD8+ T lymphocytes, as determined by depleting these cells by a panning procedure with an anti-CD8 monoclonal antibody. MHC-restricted CD8+ CTL in unstimulated PBMC from infected horses caused significant specific lysis of autologous EK cells infected with recombinant vaccinia viruses expressing EIAV genes, either env or gag plus 5\u27 pol. The EIAV-specific MHC-restricted CD8+ CTL were detected in two EIAV-infected horses within a few days after plasma viremia occurred and were present after viremia was terminated. The detection of these immune effector cells in EIAV-infected horses permits further studies to determine their in vivo role

Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses

Efficacious lentiviral vaccines designed to induce cytotoxic T lymphocytes (CTL) in outbred populations with a diverse repertoire of MHC class I molecules should contain or express multiple viral proteins. To determine the equine infectious anaemia virus (EIAV) proteins with epitopes most frequently recognized by CTL from seven horses infected for 0.5 to 7 years, retroviral vector-transduced target cells expressing viral proteins were used in CTL assays. Gag p15 was recognized by CTL from 100% of these infected horses. p26 was recognized by CTL from 86%, SU and the middle third of Pol protein were each recognized by 43%, TM by 29%, and S2 by 14%. Based on these results, it is likely that a construct expressing the 359 amino acids constituting p15 and p26 would contain epitopes capable of stimulating CTL in most horses

Proteomics of Primary Cilia by Proximity Labeling.

While cilia are recognized as important signaling organelles, the extent of ciliary functions remains unknown because of difficulties in cataloguing proteins from mammalian primary cilia. We present a method that readily captures rapid snapshots of the ciliary proteome by selectively biotinylating ciliary proteins using a cilia-targeted proximity labeling enzyme (cilia-APEX). Besides identifying known ciliary proteins, cilia-APEX uncovered several ciliary signaling molecules. The kinases PKA, AMPK, and LKB1 were validated as bona fide ciliary proteins and PKA was found to regulate Hedgehog signaling in primary cilia. Furthermore, proteomics profiling of Ift27/Bbs19 mutant cilia correctly detected BBSome accumulation inside Ift27(-/-) cilia and revealed that β-arrestin 2 and the viral receptor CAR are candidate cargoes of the BBSome. This work demonstrates that proximity labeling can be applied to proteomics of non-membrane-enclosed organelles and suggests that proteomics profiling of cilia will enable a rapid and powerful characterization of ciliopathies