Effects of DDT and permethrin on rat hepatocytes cultivated in microfluidic biochips: Metabolomics and gene expression study

Dichlorodiphenyl-trichloroethane (DDT) and permethrin (PMT) are amongst most prevalent pesticides in the environment. Although their toxicity has been extensively studied, molecular mechanisms and metabolic effects remain unclear, including in liver where their detoxification occurs. Here, we used metabolomics, coupled to RT-qPCR analysis, to examine effects of DDT and PMT on hepatocytes cultivated in biochips. At 150 μM, DDT caused cell death, cytochrome P450 induction and modulation of estrogen metabolism. Metabolomics analysis showed an increase in some lipids and sugars after 6 h, and a decrease in fatty acids (tetradecanoate, octanoate and linoleate) after 24 h exposure. We also found a change in expression associated with genes involved in hepatic estrogen, lipid, and sugar metabolism. PMT at 150 μM perturbed lipid/sugar homeostasis and estrogen signaling pathway, between 2 and 6 h. After 24 h, lipids and sugars were found to decrease, suggesting continuous energy demand to detoxify PMT. Finally, at 15 μM, DDT and PMT appeared to have a small effect on metabolism and were detoxified after 24 h. Our results show a time-dependent perturbation of sugar/lipid homeostasis by DDT and PMT at 150 μM. Furthermore, DDT at high dose led to cell death, inflammatory response and oxidative stress.The study was supported by the French Agency for Food, Environmental and Occupational Health & Safety (ANSES, project IMITOMICS-N°EST-2014/1/093). The molds to fabricate the PDMS devices used in this study were built by the LAAS in the frame of the RENATECH French network

The 474-Kilobase-Pair Complete Genome Sequence of CeV-01B, a Virus Infecting Haptolina (Chrysochromulina) ericina(Prymnesiophyceae)

We report the complete genome sequence of CeV-01B, a large double-stranded DNA virus infecting the unicellular marine phytoplankton Haptolina (formerly Chrysochromulina) ericina. CeV-01B and its closest relative Phaeocystis globosa virus define an emerging subclade of the Megaviridae family with smaller genomes and particles than the originally described giant Mimiviridae infecting Acanthamoeba

Control of replication of hepatitis B and C virus improves patient and graft survival in kidney transplantation

International audienceBackground & aims: Before antiviral therapy, kidney transplant recipients infected with hepatitis B virus (HBV) or hepatitis C virus (HCV) had poor outcomes. Since the 90s, nucleos(t)ide analogues have been widely used in HBV-infected patients, while interferon-based therapy was rarely used in HCV-infected patients. The aim of this study was to assess the impact of HBV and HCV on patient and graft survival, according to viral replication status.Methods: Data from January 1993 to December 2010 were extracted from the French national database CRISTAL. A total of 31,433 kidney transplant recipients were included, of whom 575, 1,060 and 29,798 had chronic hepatitis B, C, or were not infected, respectively.Results: Ten-year survival was lower in HCV-infected (71.3%) than in HBV-infected (81.2%, p = 0.0004) or non-infected kidney transplant recipients (82.7%, p <0.0001). Ten-year kidney graft survival was lower in HCV-infected (50.6%) than in HBV-infected (62.3%, p <0.0001) or non-infected kidney transplant recipients (64.7%, p <0.0001). A random analysis of the medical records of 184 patients with HBV and 504 patients with HCV showed a control of viral replication in 94% and 35% of cases, respectively. Ten-year patient and graft survival in patients with detectable HCV RNA was lower than in their matching controls. Conversely, patients with HCV and undetectable HCV RNA had higher 10-year survival than their matched controls without significant differences in graft survival.Conclusions: Chronic HBV infection does not impact 10-year patient and kidney graft survival thanks to control of viral replication with nucleos(t)ide analogues. In kidney transplant recipients infected with HCV, patients with detectable RNA had worse outcomes, whereas the outcomes of those with undetectable RNA were at least as good as non-infected patients. Thus, direct-acting antivirals should be systematically offered to HCV-infected patients.Lay summary: Previously, infections with hepatitis B or hepatitis C virus led to poor outcomes in kidney transplant recipients. However, the outcomes of kidney transplants in patients with viral suppression are as good as those for kidney transplants in non-infected patients. Antiviral therapy should be systematically proposed to hepatitis B and/or hepatitis C-infected kidney transplant recipients or candidates to prevent the deleterious hepatic and extrahepatic impact of chronic viral replication. Recent access to direct-acting antivirals in patients with hepatitis C virus and renal dysfunction provides exciting new opportunities

Control of replication of hepatitis B and C virus improves patient and graft survival in kidney transplantation

International audienceBackground & aims: Before antiviral therapy, kidney transplant recipients infected with hepatitis B virus (HBV) or hepatitis C virus (HCV) had poor outcomes. Since the 90s, nucleos(t)ide analogues have been widely used in HBV-infected patients, while interferon-based therapy was rarely used in HCV-infected patients. The aim of this study was to assess the impact of HBV and HCV on patient and graft survival, according to viral replication status.Methods: Data from January 1993 to December 2010 were extracted from the French national database CRISTAL. A total of 31,433 kidney transplant recipients were included, of whom 575, 1,060 and 29,798 had chronic hepatitis B, C, or were not infected, respectively.Results: Ten-year survival was lower in HCV-infected (71.3%) than in HBV-infected (81.2%, p = 0.0004) or non-infected kidney transplant recipients (82.7%, p <0.0001). Ten-year kidney graft survival was lower in HCV-infected (50.6%) than in HBV-infected (62.3%, p <0.0001) or non-infected kidney transplant recipients (64.7%, p <0.0001). A random analysis of the medical records of 184 patients with HBV and 504 patients with HCV showed a control of viral replication in 94% and 35% of cases, respectively. Ten-year patient and graft survival in patients with detectable HCV RNA was lower than in their matching controls. Conversely, patients with HCV and undetectable HCV RNA had higher 10-year survival than their matched controls without significant differences in graft survival.Conclusions: Chronic HBV infection does not impact 10-year patient and kidney graft survival thanks to control of viral replication with nucleos(t)ide analogues. In kidney transplant recipients infected with HCV, patients with detectable RNA had worse outcomes, whereas the outcomes of those with undetectable RNA were at least as good as non-infected patients. Thus, direct-acting antivirals should be systematically offered to HCV-infected patients.Lay summary: Previously, infections with hepatitis B or hepatitis C virus led to poor outcomes in kidney transplant recipients. However, the outcomes of kidney transplants in patients with viral suppression are as good as those for kidney transplants in non-infected patients. Antiviral therapy should be systematically proposed to hepatitis B and/or hepatitis C-infected kidney transplant recipients or candidates to prevent the deleterious hepatic and extrahepatic impact of chronic viral replication. Recent access to direct-acting antivirals in patients with hepatitis C virus and renal dysfunction provides exciting new opportunities

An electronic signature of hydrolysation in the X-ray absorption spectrum of aqueous formaldehyde

Formaldehyde in aqueous solution is hydrolysed and forms methanediol. Using X-ray absorption spectroscopy we show that the hydrolysation product can be identified by a distinct electronic signature in the spectra. This is manifested by the disappearance of the oxygen 1s -> pi* absorption line. The X-ray absorption spectrum of aqueous formaldehyde is compared with those of the structurally similar formamide and urea, which are in contrast not hydrolysed in aqueous solution. We thereby demonstrate the exceptional sensitivity and simplicity of the technique to monitor this fundamental process in the aqueous phase. (c) 2008 Elsevier B.V. All rights reserved

Projet OGIVE. Outils d'aide à la Gestion et à la Valorisation des Ecosystèmes conchylicoles de Basse-Normandie. Rapport final de phase III (2011-2013)

L’objectif général du projet OGIVE est d’acquérir des connaissances sur le fonctionnement des écosystèmes conchylicoles de Basse-Normandie dans le but d’établir une plateforme de données communes à l’ensemble des partenaires du projet. Ces connaissances sont ensuite utilisées pour développer des outils permettant de fournir des avis et expertises aux gestionnaires du Domaine Public Maritime. Le projet a ainsi pour objectif de contribuer au développement durable de la filière conchylicole en Basse-Normandie en optimisant la production conchylicole et en préservant la qualité environnementale des écosystèmes qui supportent cette activité. Le projet OGIVE a été structuré initialement autour de trois actions : 1) Acquisition de données : cette action a le double objectif d’assurer une veille sur l’acquisition de données classiques (évolution de l’environnement, performances d’élevage des filtreurs) et de mettre en place de nouvelles expérimentations pour compléter le champ de nos connaissances. En plus de l’écosystème conchylicole en mer, cette acquisition peut également concerner des données amont (bassins versants) & aval (socio-économie de l’activité conchylicole. 2) Développement d’outils : Il s’agit principalement d’outils de modélisation couplant les processus physiques & biologiques. Le transfert d’information cartographique est également abordé. 3) Livrables : mise en œuvre des outils pour de la gestion, transfert de connaissances, valorisation scientifique & technique. Le présent rapport est dédié à la présentation des résultats acquis au cours de la phase III du projet (2011-2013). Il constitue ainsi un rapport de fin de contrat pour la Direction des Pêches Maritimes et de l’Aquaculture (Fonds FEP, convention n°37504 pour la période 2011-2013) et de l’Agence de l’Eau Seine-Normandie avec qui la contractualisation a été établie sur les 2 dernières années de la troisième phase (convention n°1033671(1) 2012 pour la période janvier 2012 - septembre 2013). La troisième phase du projet a permis de poursuivre des tâches d’acquisition de nouvelles données : finalisation de l’évaluation des biomasses en élevage réalisée en 2011, poursuite du réseau SUMO concernant les mortalités d’huîtres adultes en baie des Veys et identification de nouveaux sites propices au développement de l’activité conchylicole avec le site de Fermanville dans le nord Cotentin. Par ailleurs, un important travail a été réalisé sur le secteur mytilicole de l’Ouest Cotentin qui couvre une campagne d’acquisition de données et des développements en modélisation écosystémique. Enfin, un portail régional d’accès aux données produites dans le cadre du projet OGIVE a également été créé sous Sextant

Auger Electron Spectroscopy as a Probe of the Solution of Aqueous Ions

Aqueous potassium chloride has been studied by synchrotron-radiation excited core-level photoelectron and Auger electron spectroscopy. In the Auger spectrum of the potassium ion, the main feature comprises the final states where two outer valence holes are localized on potassium. This spectrum exhibits also another feature at a higher kinetic energy which is related to final states where outer valence holes reside on different subunits. Through ab initio calculations for microsolvated clusters, these subunits have been assigned as potassium ions and the surrounding water molecules. The situation is more complicated in the Auger spectrum of the chloride anion. One-center and multicenter final states are present here as well but overlap energetically