Dynamic Release of Bending Stress in Short dsDNA by Formation of a Kink and Forks

Bending with high curvature is one of the major mechanical properties of double-stranded DNA (dsDNA) that is essential for its biological functions. The emergence of a kink arising from local melting in the middle of dsDNA has been suggested as a mechanism of releasing the energy cost of bending. Herein, we report that strong bending induces two types of short dsDNA deformations, induced by two types of local melting, namely, a kink in the middle and forks at the ends, which we demonstrate using D-shaped DNA nanostructures. The two types of deformed dsDNA structures dynamically interconvert on a millisecond timescale. The transition from a fork to a kink is dominated by entropic contribution (anti-Arrhenius behavior), while the transition from a kink to a fork is dominated by enthalpic contributions. The presence of mismatches in dsDNA accelerates kink formation, and the transition from a kink to a fork is removed when the mismatch size is three base pairs.1157Ysciescopu

Multi-pulse addressing of a Raman quantum memory: configurable beam splitting and efficient readout

Quantum memories are vital to the scalability of photonic quantum information processing (PQIP), since the storage of photons enables repeat-until-success strategies. On the other hand the key element of all PQIP architectures is the beam splitter, which allows to coherently couple optical modes. Here we show how to combine these crucial functionalities by addressing a Raman quantum memory with multiple control pulses. The result is a coherent optical storage device with an extremely large time-bandwidth product, that functions as an array of dynamically configurable beam splitters, and that can be read out with arbitrarily high efficiency. Networks of such devices would allow fully scalable PQIP, with applications in quantum computation, long-distance quantum communications and quantum metrology.Comment: 4 pages, 3 figure

A brief cognitive behavioural intervention for regular amphetamine users. A treatment guide.

A brief intervention using motivational and cognitive behavioural approaches to help change drug use. Also offer alternative brief interventions for clients not suited to the current approach. This manual is divided into five sections: Section 1. Context • Key points from the National Drug Strategy Monograph No 51. Models of Intervention and Care for Psychostimulant Users are included to present the evidence supporting this type of intervention for regular amphetamine users. • A flow-chart to place the intervention in a treatment context. Section 2. Brief background to the study and summary of results of evaluation • A brief description of how the study was developed, undertaken and evaluated. • A brief description of the evaluation outcome data (detailed results will be published separately). Section 3. The intervention • The CBT intervention is presented in a clear and easy to use format for practitioners. Section 4. Suggested alternative brief interventions for those not suitable for the current intervention • This section provides an overview of recommendations for alternative interventions for psychostimulant users who are unsuitable for the CBT intervention (e.g. those who are not considering change, experimental users etc). Section 5. Other available resources • This section lists a range of other resources that are currently available for practitioners working with psychostimulant users. This treatment guide has not been designed to stand alone. Rather, practitioners are encouraged to: 1. Acquaint themselves with the current research and clinical literature. The recently completed monograph Models of Intervention and Care for Psychostimulant Users is an excellent resource for current evidence supporting practice in this area. 2. Undertake training in CBT and motivational enhancement techniques if unfamiliar with these approaches. 3. Obtain ongoing clinical supervision

Resource recovery using whey permeate to cultivate Phellinus linteus mycelium: Solid-state and submerged liquid fermentation

The growth characteristics of Phellinus linteus mycelium were assessed and compared under solid-state fermentation (SSF) and submerged liquid fermentation (SLF) systems on whey permeate medium. Response surface methodology was used to investigate the growth rates of mycelia under various conditions of operating temperature (T-O), initial pH, and substrate concentration ([S]). The optimal growth conditions of P. linteus mycelium were determined to be 26.1 degrees C, pH 4.6, and 60.3 g of lactose/L in the SSF system, and 29.0 degrees C, pH 5.0, and 65.3 g of lactose/L in the SLF system. The maximum growth rates were predicted to be 1.92 +/- 0.01 mm/d in SSF and 192.1 +/- 0.0 mg/L per day in SLF. Random trials were conducted to experimentally validate the evaluated optimal conditions. The differences between the modeled and observed values were only 5.3% in the SSF system and 6.1% in the SLF system. Significant engineering factors differed between the fermentation techniques; T-O was significant in both cultivation systems, whereas initial pH was significant in SSF but [51 was significant in SLF. Our findings can be used to guide the operation of the bioconversion process for cultivating P. linteus mycelium using whey permeate wastewater.1111Ysciescopu

Enhancing Stratified Graph Sampling Algorithms based on Approximate Degree Distribution

Sampling technique has become one of the recent research focuses in the graph-related fields. Most of the existing graph sampling algorithms tend to sample the high degree or low degree nodes in the complex networks because of the characteristic of scale-free. Scale-free means that degrees of different nodes are subject to a power law distribution. So, there is a significant difference in the degrees between the overall sampling nodes. In this paper, we propose an idea of approximate degree distribution and devise a stratified strategy using it in the complex networks. We also develop two graph sampling algorithms combining the node selection method with the stratified strategy. The experimental results show that our sampling algorithms preserve several properties of different graphs and behave more accurately than other algorithms. Further, we prove the proposed algorithms are superior to the off-the-shelf algorithms in terms of the unbiasedness of the degrees and more efficient than state-of-the-art FFS and ES-i algorithms.Comment: 10 pages, 23 figures, the concept of approximate degree distribution, scale-free networks, graph sampling methods, stratified technolog

Modeling Resolution of Resources Contention in Synchronous Data Flow Graphs

Synchronous Data Flow graphs are widely adopted in the designing of streaming applications, but were originally formulated to describe only how an application is partitioned and which data are exchanged among different tasks. Since Synchronous Data Flow graphs are often used to describe and evaluate complete design solutions, missing information (e.g., mapping, scheduling, etc.) has to be included in them by means of further actors and channels to obtain accurate evaluations. To address this issue preserving the simplicity of the representation, techniques that model data transfer delays by means of ad-hoc actors have been proposed, but they model independently each communication ignoring contentions. Moreover, they do not usually consider at all delays due to buffer contentions, potentially overestimating the throughput of a design solution. In this paper a technique to extend Synchronous Data Flow graphs by adding ad-hoc actors and channels to model resolution of resources contentions is proposed. The results show that the number of added actors and channels is limited but that they can significantly increase the Synchronous Data Flow graph accuracy

Real-time submillisecond single-molecule FRET dynamics of freely diffusing molecules with liposome tethering

Single-molecule fluorescence resonance energy transfer (smFRET) is one of the powerful techniques for deciphering the dynamics of unsynchronized biomolecules. However, smFRET is limited in its temporal resolution for observing dynamics. Here, we report a novel method for observing real-time dynamics with submillisecond resolution by tethering molecules to freely diffusing 100-nm-sized liposomes. The observation time for a diffusing molecule is extended to 100 ms with a submillisecond resolution, which allows for direct analysis of the transition states from the FRET time trace using hidden Markov modelling. We measure transition rates of up to 1,500 s(-1) between two conformers of a Holliday junction. The rapid diffusional migration of Deinococcus radiodurans single-stranded DNA-binding protein (SSB) on single-stranded DNA is resolved by FRET, faster than that of Escherichia coli SSB by an order of magnitude. Our approach is a powerful method for studying the dynamics and movements of biomolecules at submillisecond resolution.open112420sciescopu

A cross validation of Consumer-Based Brand Equity (CBBE) with Private Labels in Spain

Molinillo,S., Ekinci, Y., Japutra, A. (2014)'A cross validation of Consumer-Based Brand Equity (CBBE) with Private Labels in Spain'. in Martínez-López, Gázquez-Abad, J.C. and Sethuraman, R. J.A. (eds.) Advances in National Brand and Private Label Marketing. Second International Conference, 2015. Springer Proceedings in Business and Economics, pp. 113-125In recent years a number of Consumer-Based Brand Equity (CBBE) models and measurement scales have been introduced in the branding literature. However, examinations of brand equity in Private Labels (PL) are rather limited. This study aims to compare the validity of the two prominent CBBE models those introduced by Yoo and Donthu (2001) and Nam et al. (2011). In order to test the models and make this comparison, the study collected data from 236 respondents who rated private labels in Spain. A list of 30 different fashion and sportswear PL was introduced to respondents. These brands do not make any reference to the retail store in which they are sold. Research findings suggest that the extended CBBE model introduced by Nam et al. (2011) and Ciftci et al. (2014) is more reliable and valid than Yoo and Donthu’s model for assessing PL. Theoretical contributions and managerial implications are discussed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Use of an Anaerobic Chamber Environment for the Assay of Endogenous Cellular Protein-Tyrosine Phosphatase Activities

Protein-tyrosine phosphatases (PTPases) have a catalytic cysteine residue whose reduced state is integral to the reaction mechanism. Since exposure to air can artifactually oxidize this highly reactive thiol, PTPase assays have typically used potent reducing agents to reactivate the enzymes present; however, this approach does not allow for the measurement of the endogenous PTPase activity directly isolated from the in vivo cellular environment. Here we provide a method for using an anaerobic chamber to preserve the activity of the total PTPase complement in a tissue lysate or of an immunoprecipitated PTPase homolog to characterize their endogenous activation state. Comparison with a sample treated with biochemical reducing agents allows the determination of the activatable (reducible) fraction of the endogenous PTPase pool