Protein refolding in an oscillatory flow reactor

We demonstrate that an oscillatory flow reactor is a viable reactor for protein refolding via direct dilution. The mixing characteristics of the oscillatory flow reactor are well described and controllable and, importantly, can be scaled-up to process scale without a loss of mixing efficiency. This makes the oscillatory flow reactor an attractive alternative to conventional stirred-tank reactors for process-scale renaturation

XML schema design and management for e-Government data interoperability

Open Access JournalThis journal issue entitled: ECEG 2009postprin

Elastin is Localised to the Interfascicular Matrix of Energy Storing Tendons and Becomes Increasingly Disorganised With Ageing

Tendon is composed of fascicles bound together by the interfascicular matrix (IFM). Energy storing tendons are more elastic and extensible than positional tendons; behaviour provided by specialisation of the IFM to enable repeated interfascicular sliding and recoil. With ageing, the IFM becomes stiffer and less fatigue resistant, potentially explaining why older tendons become more injury-prone. Recent data indicates enrichment of elastin within the IFM, but this has yet to be quantified. We hypothesised that elastin is more prevalent in energy storing than positional tendons, and is mainly localised to the IFM. Further, we hypothesised that elastin becomes disorganised and fragmented, and decreases in amount with ageing, especially in energy storing tendons. Biochemical analyses and immunohistochemical techniques were used to determine elastin content and organisation, in young and old equine energy storing and positional tendons. Supporting the hypothesis, elastin localises to the IFM of energy storing tendons, reducing in quantity and becoming more disorganised with ageing. These changes may contribute to the increased injury risk in aged energy storing tendons. Full understanding of the processes leading to loss of elastin and its disorganisation with ageing may aid in the development of treatments to prevent age related tendinopathy

Using AdaBoost-based Multiple Functional Neural Fuzzy Classifiers Fusion for Classification Applications

© The Authors, published by EDP Sciences, 2018. In this study, two intelligent classifiers, the AdaBoost-based incremental functional neural fuzzy classifier (AIFNFC) and the AdaBoost-based fixed functional neural fuzzy classifier (AFFNFC), are proposed for solving the classification problems. The AIFNFC approach will increase the amount of functional neural fuzzy classifiers based on the corresponding error during the training phase; while the AFNFC approach is equipped with a fixed amount of functional neural fuzzy classifiers. Then, the weights of AdaBoost procedure are assigned for classifiers. The proposed methods are applied to different classification benchmarks. Results of this study demonstrate the effectiveness of the proposed AIFNFC and AFFNFC methods

Calycosin and Formononetin Induce Endothelium-Dependent Vasodilation by the Activation of Large-Conductance Ca2+-Activated K+ Channels (BKCa)

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C. elegans maximum velocity correlates with healthspan and is maintained in worms with an insulin receptor mutation

Ageing is marked by physical decline. Caenorhabditis elegans is a valuable model for identifying genetic regulatory mechanisms of ageing and longevity. Here we report a simple method to assess C. elegans' maximum physical ability based on the worms' maximum movement velocity. We show maximum velocity declines with age, correlates well with longevity, accurately reports movement ability and, if measured in mid-adulthood, is predictive of maximal lifespan. Contrary to recent findings, we observe that maximum velocity of worm with mutations in daf-2(e1370) insulin/IGF-1 signalling scales with lifespan. Because of increased odorant receptor expression, daf-2(e1370) mutants prefer food over exploration, causing previous on-food motility assays to underestimate movement ability and, thus, worm health. Finally, a disease-burden analysis of published data reveals that the daf-2(e1370) mutation improves quality of life, and therefore combines lifespan extension with various signs of an increased healthspan.114232Ysciescopu

NGC 5775: Anatomy of a disk-halo interface

We present the first high-resolution study of the disk-halo interface in an edge-on galaxy (NGC 5775) in which every component of the interstellar medium is represented and resolved (though not all to the same resolution). New single-dish CO J=2-1 and CO J=1-0 data, ROSAT X-ray data, and HIRES IRAS data are presented along with HI data which emphasizes the high latitude features. In conjunction with previously published radio continuum (6 and 20 cm) and H data, we find spatial correlations between various ISM components in that all components of the ISM are present in the disk-halo features (except for CO for which there is insufficient spatial coverage). The HI features extend to ~7 kpc above the plane, form loops in position-position space, in one case, form a loop in position-velocity space, and are also observed over a large velocity range. This implies that the disk-halo features represent expanding supershells. However, the shells may be incomplete and partially open-topped, suggesting that we are observing the breakup of the supershells as they traverse the disk-halo interface. There is some evidence for acceleration with z and both redshifted and blueshifted velocities are present, although the gas which is lagging with respect to galactic rotation dominates. The radio continuum spectral index is flatter around the shell rims and we show that this cannot be due to a contribution from thermal gas but rather is due to intrinsic flattening of the non-thermal spectral index, suggesting that shocks may be important in these regions. The H emission is located interior to the HI. For feature F3, the H emission forms the interior skin of the HI shell, yet there appears to be a minimum of in-disk star formation immediately below the feature. We present a picture of a typical HI supershell which accelerates and breaks up through the disk-halo interface. Such a feature is likely internally generated via an energetic event in the disk

Impact of disturbance on population and genetic structure of tropical forest trees

Effects of selective logging on the population and genetic structure of Shorea curtisii, Dryobalanops aromatica and Scaphium macropodum were examined via two approaches:(1) to investigate the immediate effects by studying a same population before and after logging and,(2) to determine the long term effects by comparing regenerated stands with an adjacent unlogged stand, assuming that they were genetically identical before logging. Reduction in basal area of trees from different size classes due to a single ..