A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish.

Zebrafish are a popular vertebrate model for human neurological disorders and drug discovery. Although fecundity, breeding convenience, genetic homology and optical transparency have been key advantages, laborious and invasive procedures are required for electrophysiological studies. Using an electrode-integrated microfluidic system, here we demonstrate a novel multichannel electrophysiology unit to record multiple zebrafish. This platform allows spontaneous alignment of zebrafish and maintains, over days, close contact between head and multiple surface electrodes, enabling non-invasive long-term electroencephalographic recording. First, we demonstrate that electrographic seizure events, induced by pentylenetetrazole, can be reliably distinguished from eye or tail movement artifacts, and quantifiably identified with our unique algorithm. Second, we show long-term monitoring during epileptogenic progression in a scn1lab mutant recapitulating human Dravet syndrome. Third, we provide an example of cross-over pharmacology antiepileptic drug testing. Such promising features of this integrated microfluidic platform will greatly facilitate high-throughput drug screening and electrophysiological characterization of epileptic zebrafish

Reversible Self-Actuated Thermo-Responsive Pore Membrane.

Smart membranes, which can selectively control the transfer of light, air, humidity and temperature, are important to achieve indoor climate regulation. Even though reversible self-actuation of smart membranes is desirable in large-scale, reversible self-regulation remains challenging. Specifically, reversible 100% opening/closing of pore actuation showing accurate responsiveness, reproducibility and structural flexibility, including uniform structure assembly, is currently very difficult. Here, we report a reversible, thermo-responsive self-activated pore membrane that achieves opening and closing of pores. The reversible, self-actuated thermo-responsive pore membrane was fabricated with hybrid materials of poly (N-isopropylacrylamide), (PNIPAM) within polytetrafluoroethylene (PTFE) to form a multi-dimensional pore array. Using Multiphysics simulation of heat transfer and structural mechanics based on finite element analysis, we demonstrated that pore opening and closing dynamics can be self-activated at environmentally relevant temperatures. Temperature cycle characterizations of the pore structure revealed 100% opening ratio at T = 40 °C and 0% opening ratio at T = 20 °C. The flexibility of the membrane showed an accurate temperature-responsive function at a maximum bending angle of 45°. Addressing the importance of self-regulation, this reversible self-actuated thermo-responsive pore membrane will advance the development of future large-scale smart membranes needed for sustainable indoor climate control

End-to-end Neural Coreference Resolution

We introduce the first end-to-end coreference resolution model and show that it significantly outperforms all previous work without using a syntactic parser or hand-engineered mention detector. The key idea is to directly consider all spans in a document as potential mentions and learn distributions over possible antecedents for each. The model computes span embeddings that combine context-dependent boundary representations with a head-finding attention mechanism. It is trained to maximize the marginal likelihood of gold antecedent spans from coreference clusters and is factored to enable aggressive pruning of potential mentions. Experiments demonstrate state-of-the-art performance, with a gain of 1.5 F1 on the OntoNotes benchmark and by 3.1 F1 using a 5-model ensemble, despite the fact that this is the first approach to be successfully trained with no external resources.Comment: Accepted to EMNLP 201

Efficacy of Using Virtual Reality to Reduce Perceived Exertion During Therapy When Compared to Traditional OT Intervention

This project looked at the differences in perceived and actual exertion in virtual reality therapy interventions and upper extremity ergometer. The goal of this project was to quantify the efficacy of virtual reality interventions as an alternative to more traditional upper extremity interventions. This project wanted to address the lack of research focused on perceived exertion in virtual reality interventions. The student objectives focused on conducting research, familiarizing oneself with the use of virtual reality in the in-patient setting, and dissemination of the results. This study found that there was no significant difference in perceived exertion scores, changes in heart rate, or changes in systolic blood pressure between virtual reality interventions and upper extremity ergometer interventions. However, virtual reality interventions were associated with a slight decrease in perceived exertion and a slight increase in heart rate and systolic blood pressure changes when compared to the upper extremity ergometer. This project shows that virtual reality interventions may be a viable alternative to more traditional upper extremity interventions as it does not show any adverse effects in perceived exertion

Synthesis method of asymmetric gold particles.

Asymmetric particles can exhibit unique properties. However, reported synthesis methods for asymmetric particles hinder their application because these methods have a limited scale and lack the ability to afford particles of varied shapes. Herein, we report a novel synthetic method which has the potential to produce large quantities of asymmetric particles. Asymmetric rose-shaped gold particles were fabricated as a proof of concept experiment. First, silica nanoparticles (NPs) were bound to a hydrophobic micro-sized polymer containing 2-chlorotritylchloride linkers (2-CTC resin). Then, half-planar gold particles with rose-shaped and polyhedral structures were prepared on the silica particles on the 2-CTC resin. Particle size was controlled by the concentration of the gold source. The asymmetric particles were easily cleaved from the resin without aggregation. We confirmed that gold was grown on the silica NPs. This facile method for synthesizing asymmetric particles has great potential for materials science

International Law And Antipersonnel Land Mines

Antipersonnel (A/P) land mines are devastating weapons not only during, but also after, warfare or armed conflicts. There still exist an estimated 85 million mines, or one for every 50 people on earth, scattered in 62 countries that kill and maim some 26,000 innocent civilians each year. In Cambodia and Angola, for example, there are more than 30,000 and 20,000 amputees, respectively, who are victims of mine incidents