Educators’ Use and Views of Simulations as Teaching Tools within a Discipline: The Example of Hospitality and Tourism

This Scholarship of Teaching and Learning study investigated hospitality faculty members’ perceptions and use of industry-related simulations in hospitality and tourism education to help the authors understand how simulations are and can be used as educational tools. Using learning engagement theory as a framework, respondents to a survey perceived that simulations help students develop decision-making skills, increase problem-solving skills, integrate knowledge from other classes, learn to work with others, and link theory to practice, and noted that simulations are fun to use. A significant positive relationship was found between the number of semesters educators have used simulations and their satisfaction with the simulations. Perceived drawbacks to the use of simulations included that they are costly, time-consuming, too complex, and lack realism and validity. Based on the findings, suggestions are made for improving the use of simulations in hospitality and tourism education, and limitations and ideas for further research are offered

The genetics of colored sequence synesthesia: Evidence of linkage to chromosome 16q and genetic heterogeneity for the condition

Synesthesia is a perceptual condition in which normal sensory stimulation can trigger anomalous sensory experiences. For example, synesthetes may experience colors in response to sounds, tastes in response to words, or smells in response to touch. We here focus on colored sequence synesthesia, in which color experiences are triggered by learned ordinal sequences such as letters, numbers, weekdays and months. Although synesthesia has been noted in the scientific literature for over a century, it is understood only at the level of the phenomenology, and not at the molecular and neural levels. We have performed a linkage analysis to identify the first genetic loci responsible for the increased neural crosstalk underlying colored sequence synesthesia. Our analysis has identified a 23 MB region on chromosome 16 as a putative locus for the trait. Our data provide the first step in understanding neural crosstalk from its molecular basis to its behavioral consequences, opening a new inroad into the understanding of the multisensory brain

A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons.

Nanobodies (nAbs) are small, minimal antibodies that have distinct attributes that make them uniquely suited for certain biomedical research, diagnostic and therapeutic applications. Prominent uses include as intracellular antibodies or intrabodies to bind and deliver cargo to specific proteins and/or subcellular sites within cells, and as nanoscale immunolabels for enhanced tissue penetration and improved spatial imaging resolution. Here, we report the generation and validation of nAbs against a set of proteins prominently expressed at specific subcellular sites in mammalian brain neurons. We describe a novel hierarchical validation pipeline to systematically evaluate nAbs isolated by phage display for effective and specific use as intrabodies and immunolabels in mammalian cells including brain neurons. These nAbs form part of a robust toolbox for targeting proteins with distinct and highly spatially-restricted subcellular localization in mammalian brain neurons, allowing for visualization and/or modulation of structure and function at those sites

Engineering Vertical Crystal Arrtays for Efficient Solar Energy Harvesting

Figure 1 - Cross-sectional scanning electron micrograph of a vertical organic semiconductor crystal array deposited from solution onto a nanoporous scaffold. Solution-processable organic semiconductors promise to drive down the cost of optoelectronic devices by affording the large-scale, high-throughput manufacturing of photo- and electrically-active layers. Achieving optimal morphologies during rapid deposition from solution, however, has proven exceedingly difficult and has thus far limited the performance of these materials. A novel method is presented to control the earliest stages of nucleation and crystallization during solution-phase deposition of small-molecule organic semiconductors. Nanoporous scaffolds were introduced to the surface of device platforms that confine organic semiconductor nucleation at the air-solution-surface interface during a continuous dip coating process. These nuclei were found to preferentially orient with their fast growth direction aligned parallel to the long axis of the pores. Subsequent crystallization proceeded beyond the scaffold to form arrays of high-density, vertical crystals with large exposed surface area. X-ray diffraction analysis revealed that the vertical crystals oriented with the p-stack direction perpendicular to the substrate surface, the optimal orientation for light absorption and charge transport in organic solar cells and other devices with a sandwich electrode configuration. The height, diameter, and spacing of these crystals were tunable by varying the scaffold geometry and deposition conditions.1 Critically, this generalizable method is compatible with continuous processing methods that will enable the large-scale manufacturing of such materials.2 Please click Additional Files below to see the full abstract

The influence of end of day silicone hydrogel daily disposable contact lens fit on ocular comfort, physiology and lens wettability

Purpose: To quantify the end-of-day silicone-hydrogel daily disposable contact lens fit and its influence of on ocular comfort, physiology and lens wettability. Methods: Thirty-nine subjects (22.1. ±. 3.5 years) were randomised to wear each of 3 silicone-hydrogel daily-disposable contact lenses (narafilcon A, delefilcon A and filcon II 3), bilaterally, for one week. Lens fit was assessed objectively using a digital video slit-lamp at 8, 12 and 16. h after lens insertion. Hyperaemia, non-invasive tear break-up time, tear meniscus height and comfort were also evaluated at these timepoints, while corneal and conjunctival staining were assessed on lens removal. Results: Lens fit assessments were not different between brands (P > 0.05), with the exception of the movement at blink where narafilcon A was more mobile. Overall, lag reduced but push-up speed increased from 8 to 12. h (P 0.05). Movement-on-blink was unaffected by wear-time (F = 0.403, P = 0.670). A more mobile lens fit with one brand did not indicate that person would have a more mobile fit with another brand (r = -0.06 to 0.63). Lens fit was not correlated with comfort, ocular physiology or lens wettability (P > 0.01). Conclusions: Among the lenses tested, objective lens fit changed between 8. h and 12. h of lens wear. The weak correlation in individual lens fit between brands indicates that fit is dependent on more than ocular shape. Consequently, substitution of a different lens brand with similar parameters will not necessarily provide comparable lens fit

Differential Timing and Coordination of Neurogenesis and Astrogenesis in Developing Mouse Hippocampal Subregions

Funding Information: Funding: This research was funded by the National Institutes of Health, grant number R35NS097370 to G.-l.M. and grant number R35NS116843 to H.S.Peer reviewedPublisher PD

Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells.

Urea cycle disorders are incurable enzymopathies that affect nitrogen metabolism and typically lead to hyperammonemia. Arginase deficiency results from a mutation in Arg1, the enzyme regulating the final step of ureagenesis and typically results in developmental disabilities, seizures, spastic diplegia, and sometimes death. Current medical treatments for urea cycle disorders are only marginally effective, and for proximal disorders, liver transplantation is effective but limited by graft availability. Advances in human induced pluripotent stem cell research has allowed for the genetic modification of stem cells for potential cellular replacement therapies. In this study, we demonstrate a universally-applicable CRISPR/Cas9-based strategy utilizing exon 1 of the hypoxanthine-guanine phosphoribosyltransferase locus to genetically modify and restore arginase activity, and thus ureagenesis, in genetically distinct patient-specific human induced pluripotent stem cells and hepatocyte-like derivatives. Successful strategies restoring gene function in patient-specific human induced pluripotent stem cells may advance applications of genetically modified cell therapy to treat urea cycle and other inborn errors of metabolism