354 research outputs found

    On the isolation of TI-plasmid from Agrobacterium tumefaciens

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    An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA, followed by Sarkosyl, after growth in the presence of carbenicillin. We also present a procedure for the isolation of the TI-plasmid on a large scale, that might be used for the mass isolation of other large plasmids which like the TI-plasmid, can not be cleared with earlier described procedures. The purity of the plasmid preparations was determined with DNA renaturation kinetics, which method has the advantage that the plasmid need not to be in the supercoiled or open circular form

    Evaluation of spectra VRE, a new chromogenic agar medium designed to screen for vancomycin-resistant Enterococcus faecalis and Enterococcus faecium

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    Spectra VRE (Remel, Lenexa, KS) is a chromogenic medium designed to recover and differentiate vancomycin-resistant Enterococcus faecium and Enterococcus faecalis (VRE). This medium was compared to bile esculin azide agar (BEAV) and was 98.2% sensitive and 99.3% specific compared to BEAV, which was 87.6% sensitive and 87.1% specific at 24 h

    Language Partners at the YWCA: UNI Spanish, English and TESOL students collaborating with English students in community ESOL classes

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    In 2016, a collaboration began between UNI faculty and students in service learning courses in the Department of Languages & Literatures and classes in English for speakers of other languages (ESOL) at the YWCA of Black Hawk County. Since that time, students in two Spanish courses (Latinos in the U.S. and Advanced Conversation & Reading) and one English linguistics course (Structure of English) have worked as language partners with adult learners of English in full semester and partial semester community-based learning projects over the course of five consecutive semesters (Spring 2016 to Spring 2018). Members of the L&L faculty, Elise DuBord, Jennifer Cooley and Caroline Ledeboer, have facilitated this collaboration with ESOL instructors and Multicultural Services Coordinators, Alejandra Huesca and Umaru Balde. As institutional partners, Department of Languages & Literatures and the YWCA have developed a positive and sustainable working relationship. Over one hundred UNI students have worked at the YWCA through coursework in their majors with approximately fifty community English students in this informal setting. These adult language learners come from a wide range of educational and language backgrounds, ranging from francophone and Portuguese-speaking Africa, Spanish-speaking Latin America, Serbo-Croatian Bosnia, and several language groups from Myanmar (Burma). Typical class sessions in the ESOL classes include informal English lessons on practical topics, such as food, shopping, the public library, and medical appointments, but the central components of this project are the communication skills and intercultural competency that all participants develop

    A multi-mRNA host-response molecular blood test for the diagnosis and prognosis of acute infections and sepsis: Proceedings from a clinical advisory panel

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    Current diagnostics are insufficient for diagnosis and prognosis of acute infections and sepsis. Clinical decisions including prescription and timing of antibiotics, ordering of additional diagnostics and level-of-care decisions rely on understanding etiology and implications of a clinical presentation. Host mRNA signatures can differentiate infectious from noninfectious etiologies, bacterial from viral infections, and predict 30-day mortality. The 29-host-mRNA blood-based InSe

    Culture and PCR detection of Haemophilus influenzae and Haemophilus haemolyticus in Australian indigenous children with bronchiectasis

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    A PCR for protein D (hpd#3) was used to differentiate nontypeable Haemophilus influenzae (NTHI) from Haemophilus haemolyticus. While 90% of nasopharyngeal specimens and 100% of lower-airway specimens from 84 Indigenous Australian children with bronchiectasis had phenotypic NTHI isolates confirmed as H. influenzae, only 39% of oropharyngeal specimens with phenotypic NTHI had H. influenzae. The nasopharynx is therefore the preferred site for NTHI colonization studies, and NTHI is confirmed as an important lower-airway pathogen
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