951 research outputs found
The Evolution of Geotechnical Earthquake Engineering Practice in North America: 1954-1994
This paper traces the evolution of geotechnical earthquake engineering practice in North America from 1954 to 1994. The development of the state-of-the-art has been shaped strongly by four areas of practice: assessment of seismic hazard, estimation of liquefaction potential, seismic response analysis of earth structures and seismic safety evaluation and remediation of existing dams with potentially liquefiable zones. Evolution of practice in each of these areas will be traced and the current state-of-the-art evaluated. Present capabilities in practice will be illustrated by examples from the areas of seismic response of dams, liquefaction potential and seismic safety evaluation and remediation of potentially liquefiable embankment dams
CD40 Ligand Expression is Defective in a Subset of Patients with Common Variable Immunodeficiency.
Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play a prominent role in the failure of CVI B cells to produce specific antibody. We have previously shown that B-cell proliferation and IgE production after stimulation with anti-CD40 and interleukin (IL) 4 were normal in 22 CVI patients evaluated, indicating that CVI B cells respond to signals delivered via CD40. Here we report that CD40 ligand (gp39) mRNA expression by activated lymphocytes from CVI patients (n = 31) as a group was significantly depressed (P \u3c 0.0001) compared with normal controls (n = 32). gp39 mRNA expression by activated lymphocytes from 13 CVI patients fell below the normal control range. T-cell surface expression of functional gp39 protein was correspondingly low in those patients with gp39 mRNA levels below normal control range and normal in patients with gp39 mRNA levels within normal control range. In CVI patients as a group, gp39 mRNA levels correlated with IL-2 mRNA levels (P \u3c 0.002, r = 0.6) and production (P \u3c 0.001, r = 0.7) but not with gene expression or production of other lymphokines evaluated, suggesting an as-yet-undetermined association between gp39 and IL-2 gene regulation. Of the 13 patients whose activated T cells exhibited gp39 mRNA expression below the normal control range, 2 had normal T-cell-derived lymphokine production, whereas the remaining 11 exhibited broader T-cell dysfunction, resulting in IL-2 deficiency, and in some patients deficient production of other lymphokines as well, reflecting a heterogeneity in the underlying mechanisms leading to depressed gp39 expression in these patients. The observation that both gene and surface expression of gp39 by activated T cells is depressed in a subgroup of CVI patients suggests that inefficient signaling via CD40 may be responsible, in part, for failure of B-cell differentiation in these patients
A strategy for the characterization of minute chromosome rearrangements using multiple color fluorescence in situ hybridization with chromosome-specific DNA libraries and YAC clones
The identification of marker chromosomes in clinical and tumor cytogenetics by chromosome banding analysis can create problems. In this study, we present a strategy to define minute chromosomal rearrangements by multicolor fluorescence in situ hybridization (FISH) with whole chromosome painting probes derived from chromosome-specific DNA libraries and Alu-polymerase chain reaction (PCR) products of various region-specific yeast artificial chromosome (YAC) clones. To demonstrate the usefulness of this strategy for the characterization of chromosome rearrangements unidentifiable by banding techniques, an 8p+ marker chromosome with two extra bands present in the karyotype of a child with multiple anomalies, malformations, and severe mental retardation was investigated. A series of seven-color FISH experiments with sets of fluorochrome-labeled DNA library probes from flow-sorted chromosomes demonstrated that the additional segment on 8p+ was derived from chromosome 6. For a more detailed characterization of the marker chromosome, three-color FISH experiments with library probes specific to chromosomes 6 and 8 were performed in combination with newly established telomeric and subtelomeric YAC clones from 6q25, 6p23, and 8p23. These experiments demonstrated a trisomy 6pter6p22 and a monosomy 8pter8p23 in the patient. The present limitations for a broad application of this strategy and its possible improvements are discusse
Genetic analysis of indefinite division in human cells: evidence for a cell senescence-related gene(s) on human chromosome 4.
Strong electron-phonon coupling in delta-phase stabilized Pu
Heat capacity measurements of the delta-phase stabilized alloy Pu-Al suggest
that strong electron-phonon coupling is required to explain the moderate
renormalization of the electronic density of states near the Fermi energy. We
calculate the heat capacity contributions from the lattice and electronic
degrees of freedom as well as from the electron-lattice coupling term and find
good overall agreement between experiment and theory assuming a dimensionless
electron-phonon coupling parameter of order unity, lambda ~ 0.8. This large
electron-phonon coupling parameter is comparable to reported values in other
superconducting metals with face-centered cubic crystal structure, for example,
Pd (lambda ~ 0.7) and Pb (lambda ~ 1.5). Further, our analysis shows evidence
of a sizable residual low-temperature entropy contribution, S_{res} ~ 0.4 k_B
(per atom). We can fit the residual specific heat to a two-level system.
Therefore, we speculate that the observed residual entropy originates from
crystal-electric field effects of the Pu atoms or from self-irradiation induced
defects frozen in at low temperatures.Comment: 9 pages, 11 figures, to appear in Phys. Rev.
Studies of Vibrational Properties in Ga Stabilized d-Pu by Extended X-ray Absorption Fine Structure
Temperature dependent extended x-ray absorption fine structure (EXAFS)
spectra were measured for a 3.3 at% Ga stabilized Pu alloy over the range T= 20
- 300 K at both the Ga K-edge and the Pu L_III-edge. The temperature dependence
of the pair-distance distribution widths, \sigma(T) was accurately modeled
using a correlated-Debye model for the lattice vibrational properties,
suggesting Debye-like behavior in this material. We obtain pair- specific
correlated-Debye temperatures, \Theta_cD, of 110.7 +/- 1.7 K and 202.6 +/- 3.7
K, for the Pu-Pu and Ga-Pu pairs, respectively. These results represent the
first unambiguous determination of Ga-specific vibrational properties in PuGa
alloys, and indicate the Ga-Pu bonds are significantly stronger than the Pu-Pu
bonds. This effect has important implications for lattice stabilization
mechanisms in these alloys.Comment: 7 pages, 4 figures, Phys. Rev. B in pres
Detailed electronic structure studies on superconducting MgB and related compounds
In order to understand the unexpected superconducting behavior of MgB
compound we have made electronic structure calculations for MgB and closely
related systems. Our calculated Debye temperature from the elastic properties
indicate that the average phonon frequency is very large in MgB compared
with other superconducting intermetallics and the exceptionally high in
this material can be explained through BCS mechanism only if phonon softening
occurs or the phonon modes are highly anisotropic. We identified a
doubly-degenerate quasi-two dimensional key-energy band in the vicinity of
along -A direction of BZ which play an important role in
deciding the superconducting behavior of this material. Based on this result,
we have searched for similar kinds of electronic feature in a series of
isoelectronic compounds such as BeB, CaB, SrB, LiBC and
MgBC and found that MgBC is one potential material from the
superconductivity point of view. There are contradictory experimental results
regarding the anisotropy in the elastic properties of MgB ranging from
isotropic, moderately anisotropic to highly anisotropic. In order to settle
this issue we have calculated the single crystal elastic constants for MgB
by the accurate full-potential method and derived the directional dependent
linear compressibility, Young's modulus, shear modulus and relevant elastic
properties. We have observed large anisotropy in the elastic properties. Our
calculated polarized optical dielectric tensor shows highly anisotropic
behavior even though it possesses isotropic transport property. MgB
possesses a mixed bonding character and this has been verified from density of
states, charge density and crystal orbital Hamiltonian population analyses
Direct Observation of Propagating Gigahertz Coherent Guided Acoustic Phonons in Free Standing Single Copper Nanowires
We report on gigahertz acoustic phonon waveguiding in free-standing single
copper nanowires studied by femtosecond transient reflectivity measurements.
The results are discussed on the basis of the semianalytical resolution of the
Pochhammer and Chree equation. The spreading of the generated Gaussian wave
packet of two different modes is derived analytically and compared with the
observed oscillations of the sample reflectivity. These experiments provide a
unique way to independently obtain geometrical and material characterization.
This direct observation of coherent guided acoustic phonons in a single
nano-object is also the first step toward nanolateral size acoustic transducer
and comprehensive studies of the thermal properties of nanowires
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