Étude in vitro du transfert intestinal des fusariotoxines majeures (interactions avec les transporteurs membranaires de la famille ABC)

Deoxynivalenol (DON), nivalenol (NIV) and zearalenone (ZEA) are fusariotoxins contaminating a large variety of cereals while growing on crops. As human and animal contamination mainly occurs by the oral route, fusariotoxins must pass through the intestinal epithelial barrier to produce any potential health effects. In vitro studies of DON and NIV transfer in Caco-2 cells has shown that these toxins are efficiently absorbed by passive diffusion and actively secreted by efflux transporters ABCB1 and ABCC2. Caco-2 and LS174T intestinal cells exposed to ZEA during 72h has lead to induction of ABCC2, ABCG2 genes and ABCB1, ABCC1 genes respectively. However, protein level and transport activity of the corresponding transporters have not been induced by ZEA. Transport of fusariotoxins as unavoidable food contaminants by ABC transporters may modify the absorption of other transporters substrates, by competition.LYON1-BU.Sciences (692662101) / SudocSudocFranceF

The mycotoxin zearalenone and its metabolites specifically interact with transporter proteins ABCC1 ABCC2 and ABCC3

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Zearalenone exposure modulates the expression of ABC transporters and nuclear receptors in pregnant rats and fetal liver

International audienceThe mycotoxin zearalenone (ZEN) is produced by a variety of Fusarium fungi and contaminates numerous cereals, fruits and vegetables. Interacting with the estrogen receptors, ZEN and reduced metabolites zearalenols cause hormonal effects in animals. Few data are available on the effects of repeated exposure to ZEN, particularly during pregnancy. The aim of our work was to assess the impact of this toxin on the expression of ABC transporters and nuclear receptors in fetal liver and pregnant rats that were exposed daily (gestation day 7-20) to 1 mg/kg ZEN. Significant variations were observed, depending on the tissue type, the tissue origin (maternal or fetal), and the time of analysis after the last exposure to ZEN (4 h or 24 h). The modulations of expression were independent of the magnitude of tissue impregnation by ZEN and its metabolites. The maternal uterus was the most sensitive tissue: Abcb1a, Abcb1b and Abcg2 mRNA and protein expressions were induced at both times, while Abcc1, Abcc3 and Esr1 mRNA and protein expressions were inhibited then induced 4 h and 24 h after exposure, respectively. In the fetal liver, Abcb1a and Esr1 protein expression was inhibited at both times, while mRNA expression was induced 24 h after the last exposure to ZEN. These results suggested that ZEN exposure could impact maternal and fetal exposure to ABC transporters substrates, and influence fetus development through nuclear receptor modulation. (c) 2012 Elsevier Ireland Ltd. All rights reserved

Effect of Zearalenone gestational exposure: modulation of placenta cell differentiation and transport function

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The anthelmintic ivermectin: a substrate of Breast-Cancer Resistant Protein (BCRP)

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