Human Metapneumovirus, Peru

We retrospectively studied 420 pharyngeal swab specimens collected from Peruvian and Argentinean patients with influenzalike illness in 2002 and 2003 for evidence of human metapneumovirus (HMPV). Twelve specimens (2.3%) were positive by multiple assays. Six specimens yielded HMPV isolates. Four of the 6 isolates were of the uncommon B1 genotype

Mechanisms for cooperative shared memory

This paper explores the complexity of implementing directory protocols by examining their mechanisms - primitive operations on directories, caches, and network interfaces. We compare the following protocols: Dir1B, Dir4B, Dir4NB, DirnNB, Dir1SW and an improved version of Dir1SW (Dir1SW+). The comparison shows that the mechanisms and mechanism sequencing of Dir1SW and Dir1SW+ are simpler than those for other protocols. We also compare protocol performance by running eight benchmarks on 32 processor systems. Simulations show that Dir1SW+'s performance is comparable to more complex directory protocols. The significant disparity in hardware complexity and the small difference in performance argue that Dir1SW+ may be a more effective use of resources. The small performance difference is attributable to two factors: the low degree of sharing in the benchmarks and Check-In/Check-Out (CICO) directives

High-throughput gene discovery in the rat

The rat is an important animal model for human diseases and is widely used in physiology. In this article we present a new strategy for gene discovery based on the production of ESTs from serially subtracted and normalized cDNA libraries, and we describe its application for the development of a comprehensive nonredundant collection of rat ESTs. Our new strategy appears to yield substantially more EST clusters per ESTs sequenced than do previous approaches that did not use serial subtraction. However, multiple rounds of library subtraction resulted in high frequencies of otherwise rare internally primed cDNAs, defining the limits of this powerful approach. To date, we have generated >200,000 3′ ESTs from >100 cDNA libraries representing a wide range of tissues and developmental stages of the laboratory rat. Most importantly, we have contributed to ∼50,000 rat UniGene clusters. We have identified, arrayed, and derived 5′ ESTs from >30,000 unique rat cDNA clones. Complete information, including radiation hybrid mapping data, is also maintained locally at http://genome.uiowa.edu/clcg.html. All of the sequences described in this article have been submitted to the dbEST division of the NCBI

Genotype Prevalence and Risk Factors for Severe Clinical Adenovirus Infection, United States 2004-2006

Recently, epidemiological and clinical data have revealed important changes with regard to clinical adenovirus infection, including alterations in antigenic presentation, geographical distribution, and virulence of the virus

The effect of time-dependent γ-pumping on buoyant magnetic structures

In this paper, we explore for the first time the interactions of the net downward, time-dependent, γ-pumping overlying an imposed layer of magnetic fluid, in a polytropic atmosphere. Our calculations show that an equipartition of energy, between the magnetic and kinetic components, must be reached for buoyancy-driven magnetic structures to rise into the pumping region. However, structures do not rise unhindered, as in a previous investigation. We show that the evolution and other features of the emerging magnetic flux structures are significantly affected by the temporal variation of the γ-pumping. The rate of emerging structures, the strength of magnetic concentrations and the extent to how far magnetic field can travel were all found to depend on the timescale of the γ-pumping

The Wisconsin Wind Tunnel: Virtual Prototyping of Parallel Computers

We have developed a new technique for evaluating cache coherent, shared-memory computers. The Wisconsin Wind Tunnel (WWT) runs a parallel shared-memory program on a parallel computer (CM-5) and uses execution-driven, distributed, discrete-event simulation to accurately calculate program execution time. WWT is a virtual prototype that exploits similarities between the system under design (the target) and an existing evaluation platform (the host). The host directly executes all target program instructions and memory references that hit in the target cache. WWT's shared memory uses the CM-5 memory's error-correcting code (ECC) as valid bits for a fine-grained extension of shared virtual memory. Only memory references that miss in the target cache trap to WWT, which simulates a cache-coherence protocol. WWT correctly interleaves target machine events and calculates target program execution time. WWT runs on parallel computers with greater speed and memory capacity than uniprocessors. WWT's simulation time decreases as target system size increases for fixed-size problems and holds roughly constant as the target system and problem scale

The Wisconsin Wind Tunnel: Virtual Prototyping of Parallel Computers

We have developed a new technique for evaluating cache coherent, shared-memory computers. The Wisconsin Wind Tunnel (WWT) runs a parallel sharedmemory program on a parallel computer (CM-5) and uses execution-driven, distributed, discrete-event simulation to accurately calculate program execution time. WWT is a virtual prototype that exploits similarities between the system under design (the target) and an existing evaluation platform (the host). The host directly executes all target program instructions and memory references that hit in the target cache. WWT's shared memory uses the CM-5 memory 's error-correcting code (ECC) as valid bits for a fine-grained extension of shared virtual memory. Only memory references that miss in the target cache trap to WWT, which simulates a cache-coherence protocol. WWT correctly interleaves target machine events and calculates target program execution time. WWT runs on parallel computers with greater speed and memory capacity than uniprocessors. WWT'..