Being lonely or using substances with friends? A cross-sectional study of Hungarian adolescents’ health risk behaviours

BACKGROUND: Studying adolescents' health risk behaviours is oddly significant in Central and Eastern European countries, where the prevalence of smoking and drinking among 14-18 year old students is significantly high. The goal of our study is to examine the role of social psychological and social behavioural variables in health risk behaviours among Hungarian adolescents. METHODS: Our sample was comprised of three high schools of Debrecen (the second largest city of Hungary). In all, 501 students filled in the questionnaire from 22 classes (14-22 years old). Students aged above 18 years were excluded for the purpose of the study, giving a total sample size of 471 high school students. Descriptive statistics and binary logistic regression analyses were conducted. RESULTS: According to our results (1) social behavioural factors (namely, smoking and alcohol use of the best friend and peer group) proved to be better predictors of adolescents' health risk behaviours as compared to the included social psychological attributes (2); among the latter ones, loneliness and shyness were negatively related with both smoking and drinking, while competitiveness was a predictor of drinking prevalence among boys. CONCLUSIONS: The findings suggest that social behavioural factors, including smoking and drinking of friends, are oddly important predictors of Hungarian adolescents' health risk behaviours. According to our results, health policy should pay more attention to peer norms related to smoking and drinking during school health promotion. Developing health protective social norms may be an indispensable component of effective health promotion in high schools

The NSF REU/RET Research on Energy Absorbing 3D Printed Polymer Structures

Energy absorption capability of structures with embedded pores depends upon the amount of voids present and their configurations/distributions. In this study, the energy absorption of acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA) structures with varying pore shapes and sizes are investigated. The research was performed by two teams comprised of High School/Middle School teachers and undergraduate students as part of National Science Foundation (NSF) sponsored Research Experience for Teacher (RET)/Research Experience for Undergraduates (REU) teams. ABS samples were fabricated by Team 1 and utilized cubic unit cells with octahedral pores while Team 2 fabricated PLA samples that utilized unit cells with spherical pores. Eight sets of samples with dimensions 25mm × 25mm × 20mm were fabricated using a Makerbot Replicator 2X for ABS samples and a Lulzbot TAZ 5 for PLA samples. Each sample incorporated a 5 × 5 × 4 array of pores. All the samples were tested in compression and energy absorption per unit material volume of all the samples up to a particular maximum load was calculated from load-deflection curves. It is observed that the specific energy absorption of PLA and ABS porous structures greatly increases with increased porosity. Copyright © 2017 by ASM

Aperçu de la pathologie animale en région Pacifique Sud. Applications à la Nouvelle-Calédonie

Energy absorption capability of structures with embedded pores depends upon the amount of voids present and their configurations/distributions. In this study, the energy absorption of acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA) structures with varying pore shapes and sizes are investigated. The research was performed by two teams comprised of High School/Middle School teachers and undergraduate students as part of National Science Foundation (NSF) sponsored Research Experience for Teacher (RET)/Research Experience for Undergraduates (REU) teams. ABS samples were fabricated by Team 1 and utilized cubic unit cells with octahedral pores while Team 2 fabricated PLA samples that utilized unit cells with spherical pores. Eight sets of samples with dimensions 25mm × 25mm × 20mm were fabricated using a Makerbot Replicator 2X for ABS samples and a Lulzbot TAZ 5 for PLA samples. Each sample incorporated a 5 × 5 × 4 array of pores. All the samples were tested in compression and energy absorption per unit material volume of all the samples up to a particular maximum load was calculated from load-deflection curves. It is observed that the specific energy absorption of PLA and ABS porous structures greatly increases with increased porosity. Copyright © 2017 by ASM

Kinetic, Mutational, and Structural Studies of the Venezuelan Equine Encephalitis Virus Nonstructural Protein 2 Cysteine Protease

The Venezuelan equine encephalitis virus (VEEV) nonstructural protein 2 (nsP2) cysteine protease (EC 3.4.22.−) is essential for viral replication and is involved in the cytopathic effects (CPE) of the virus. The VEEV nsP2 protease is a member of MEROPS Clan CN and characteristically contains a papain-like protease linked to an <i>S</i>-adenosyl-l-methionine-dependent RNA methyltransferase (SAM MTase) domain. The protease contains an alternative active site motif, ⁴⁷⁵<b>N</b>V<b><u>C</u></b>WA<b>K</b>⁴⁸⁰, which differs from papain’s (CGS²⁵<b><u>C</u></b>WAFS), and the enzyme lacks a transition state-stabilizing residue homologous to Gln-19 in papain. To understand the roles of conserved residues in catalysis, we determined the structure of the free enzyme and the first structure of an inhibitor-bound alphaviral protease. The peptide-like E64d inhibitor was found to bind beneath a β-hairpin at the interface of the SAM MTase and protease domains. His-546 adopted a conformation that differed from that found in the free enzyme; one or both of the conformers may assist in leaving group departure of either the amine or Cys thiolate during the catalytic cycle. Interestingly, E64c (200 μM), the carboxylic acid form of the E64d ester, did not inhibit the nsP2 protease. To identify key residues involved in substrate binding, a number of mutants were analyzed. Mutation of the motif residue, N475A, led to a 24-fold reduction in <i>k</i>_cat/<i>K</i>_m, and the conformation of this residue did not change after inhibition. N475 forms a hydrogen bond with R662 in the SAM MTase domain, and the R662A and R662K mutations both led to 16-fold decreases in <i>k</i>_cat/<i>K</i>_m. N475 forms the base of the P1 binding site and likely orients the substrate for nucleophilic attack or plays a role in product release. An Asn homologous to N475 is similarly found in coronaviral papain-like proteases (PLpro) of the Severe Acute Respiratory Syndrome (SARS) virus and Middle East Respiratory Syndrome (MERS) virus. Mutation of another motif residue, K480A, led to a 9-fold decrease in <i>k</i>_cat and <i>k</i>_cat/<i>K</i>_m. K480 likely enhances the nucleophilicity of the Cys. Consistent with our substrate-bound models, the SAM MTase domain K706A mutation increased <i>K</i>_m 4.5-fold to 500 μM. Within the β-hairpin, the N545A mutation slightly but not significantly increased <i>k</i>_cat and <i>K</i>_m. The structures and identified active site residues may facilitate the discovery of protease inhibitors with antiviral activity