Whistle source levels of free-ranging beluga whales in Saguenay-St. Lawrence marine park

International audienceWild beluga whistle source levels (SLs) are estimated from 52 three-dimensional (3D) localized calls using a 4-hydrophone array. The probability distribution functions of the root-mean-square (rms) SL in the time domain, and the peak, the strongest 3-dB, and 10-dB SLs from the spectrogram, were non-Gaussian. The average rms SL was 143.8 +- 6.7 dB re 1microPa at 1m. SL spectral metrics were, respectively, 145.8 +- 8 dB, 143.2 +-7.1 dB, and 138.5 +-6.9 dB re 1 microPa. 1microPa / Hz at 1m

TAC-1, a Regulator of Microtubule Length in the C. elegans Embryo

AbstractRegulation of microtubule growth is critical for many cellular processes, including meiosis, mitosis, and nuclear migration. We carried out a genome-wide RNAi screen in Caenorhabditis elegans to identify genes required for pronuclear migration, one of the first events in embryogenesis requiring microtubules. Among these, we identified and characterized tac-1 a new member of the TACC (Transforming Acidic Coiled-Coil) family [1]. tac-1(RNAi) embryos exhibit very short microtubules nucleated from the centrosomes as well as short spindles. TAC-1 is initially enriched at the meiotic spindle poles and is later recruited to the sperm centrosome. TAC-1 localization at the centrosomes is regulated during the cell cycle, with high levels during mitosis and a reduction during interphase, and is dependent on aurora kinase 1 (AIR-1), a protein involved in centrosome maturation [2]. tac-1(RNAi) embryos resemble mutants of zyg-9[3], which encodes a previously characterized centrosomal protein of the XMAP215 family and was also found in our screen. We show that TAC-1 and ZYG-9 are dependent on one another for their localization at the centrosome, and this dependence suggests that they may function together as a complex. We conclude that TAC-1 is a major regulator of microtubule length in the C. elegans embryo

Transfluthrin indoor air concentration and inhalation exposure during application of electric vaporizers

International audienceDifferent household insecticide applications via two electric vaporizers emitting transfluthrin were realized in a full-scale experimental room under controlled air exchange rate conditions. On-line high-time resolved measurements of the gas-phase concentrations of the active substance during and immediately after the spreading periods were performed with a High Sensitivity Proton-Transfer-Reaction Mass Spectrometer (HS-PTR-MS). Experimental and modelled data from the ConsExpo 4.0 software were also compared to evaluate the sources of differences. Different application scenarios were also compared. Averaged inhaled concentrations over 1h, 1week, and 5months were estimated to be 8.3, 1.8, and 1.8μg.m(-3), respectively. Corresponding margins of exposures range from 1000 to 10,000, claiming for the absence of effect. Dermal and dust ingestion pathways, although roughly estimated, seems being non-negligible. This claims for a more in-depth integrated risk assessment

Involvement of fatty acid pathways and cortical interaction of the pronuclear complex in Caenorhabditis elegans embryonic polarity.

BACKGROUND: Cell polarity is essential for many decisions made during development. While investigation of polarity-specific factors has yielded great insights into the polarization process, little is known on how these polarity-specific factors link to the basic cellular mechanisms that function in non-polarity aspects of the cell. To better understand the mechanisms that establish embryonic polarity, we investigated genes required for polarity in the one-cell C. elegans embryo that are also required for other non-polarity functions. This has led to the identification of the Pod-class of mutants that are characterized by osmosensitive embryos and defects in anterior-posterior polarity. RESULTS: Mutation in either of two loci of this class, emb-8 and pod-2, disrupts embryonic polarization and results in osmotically-sensitive embryos. Loss of emb-8, a previously uncharacterized polarity gene, causes mislocalization of PAR-3 and PAR-2 that molecularly mark the anterior and posterior cortices. emb-8 encodes NADPH-cytochrome P450 reductase, a protein supplying electrons to cytochrome P450-family enzymes, some of which catalyze fatty acid modifications. Cloning of the previously characterized polarity gene pod-2 reveals it encodes acetyl-CoA carboxylase, an enzyme that catalyzes the first step in de novo fatty acid synthesis. Depletion of fatty acid synthase, the next enzyme in the biosynthetic pathway, by RNA-interference (RNAi) also causes similar loss of one-cell polarity. Furthermore, pod-2 polarity defects can be rescued by addition of exogenous fatty acids. By following the behavior of the pronucleus in emb-8 and pod-2 mutant embryos, we demonstrate that loss of polarity correlates with impaired interaction between the pronucleus-centrosome complex and the posterior cortex. CONCLUSIONS: The characterization of emb-8 and pod-2 mutant embryos suggests that the pronucleus-centrosome complex interaction with the cortex plays a direct role in establishing polarity and that fatty acid pathways are important for this polarizing event.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

Cumulative indoor exposures to Semi-Volatile Organic Compounds (SVOCs) in France: the ECOS project.

International audienceSemivolatile organic compounds (SVOCs) are widely used indoor as plasticizers, flame retardants, or pesticides. They are present in indoor environments both in air (as gas and suspended particles) and settled dust. Thus exposition to possibly harmful agents: (phthalates, polybromodiphenylethers, organophosphorous compounds...) occurs (Mitchell et al. 2007;Wu et al. 2007). Here is presented the framework of the ECOS project, which objective is to assess domestic cumulative exposure to these substances and associated health risks

L'argent des familles : pratiques et régulations sociales en Occident aux XIXe et XXe siècles

International audienceSi la monétarisation de la vie sociale est un phénomène d’origine très ancienne, elle prend une ampleur sans précédent avec l’industrialisation massive amorcée au XIXe siècle, transformant sur son passage la manière dont les familles voient à leur subsistance et tentent d’assurer, le cas échéant, la reproduction de ressources et patrimoines aux contours divers. Le recul des économies fondées sur le monde de la terre et la montée en force d’une nouvelle institutionnalisation du travail (sous l’égide du louage d’ouvrage et du salariat) fragilisent en effet bien des ménages alors que l’idéologie libérale, au même moment, fait de l’initiative individuelle et de la propriété les piliers des rapports socioéconomiques. Ces rapports vont désormais dépendre plus étroitement du maniement de l’argent et de divers véhicules de création et fructification de la valeur, lesquels se multiplient et étendent leurs ramifications avec le développement du capitalisme. À ce titre, les familles doivent composer de plus en plus avec différents marchés dont les formes et les degrés d’institutionnalisation sont variables : pour le travail, dont le salaire devient le mode dominant de rétribution, mais aussi en ce qui a trait au crédit et à la consommation courante, au logement locatif et à la propriété résidentielle, à la protection sociale, à la rémunération de l’épargne et à l’assurance, pour ne nommer que ceux-là. De quelle manière le processus de monétarisation de la vie sociale a-t-il été vécu du point de vue des ménages et des lignées familiales aux XIXe et XXe siècles? Cette question historique d’importance a réuni une trentaine de conférenciers venus du Canada, des États-Unis et d’Europe à Trois-Rivières (Québec) en juin 2016, événement dont le présent ouvrage collectif est le résultat

Characterization of organic contaminants in hair for biomonitoring purposes

Biological monitoring is one way to assess human exposure to contaminants. Blood and urine are often used as biological matrices, but hair is an innovative and effective tool for quantifying more biomarkers over a wider exposure window. In order to improve the use of hair in exposure assessment, this article identifies relevant compounds in the literature to investigate hair contamination. Statistical analysis was performed to correlate the physical-chemical properties of the relevant compounds and their concentration levels in hair. Phthalates, pyrethroids and organophosphate flame retardants were chosen for further study of the interpretation of hair measurements for exposure assessment. No significant correlation was found between the average concentration levels in the literature and the physical-chemical properties of the selected compounds. This work also explores the properties of hair and the analytical process that may impact the quantification of organic contaminants in hair. The sample preparation method (sampling, storage, washing) were also studied and adaptations were suggested to improve the existing methods