Pengelolaan Laboratorium Kimia Kimia di SMAN 1 Kupang Nusa Tenggara Timur (NTT)

Abstract                This PKM is conducted at SMA Negeri I Kupang, which is located at JLN. Cak Doko, No. 59, Oetete Village, Oebobo District, Kupang City, NTT Province.                The output in this PKM is a well-organized chemical laboratory, producing types of laboratory administration, analyzing the increase in teacher knowledge and skills regarding laboratory administration and governance.                The result of this PKM activity was an increase in the value of the ability of teachers in laboratory management and administration which had an impact on the conditions of the chemistry laboratory at SMAN 1 Kupang on 5 governance indicators indicated by N-Gain values ​​and criteria consecutively as follows, planning indicator 0, 62 (moderate), organizing indicator 0.33 (moderate), administration indicator 0.78 (high), laboratory instrument arrangement indicator 1 (high), laboratory material structuring indicator 0.93 (high), and safety indicator 0.67 ( moderate). Thus it can be concluded that this PKM activity can improve teachers' knowledge and skills regarding laboratory governance

Increasing trend of multiple resistance and genomic mobility of Neisseria gonorrhoeae to penicillin and quinolone

A significant decline of gonorrhea incidence has been observed during the years 1990-99. However, a slight increase in the number of cases has been reported in 2000. In addition, an increase in resistant strains has been found in Thailand. In this study, 207 isolates of N. gonorrhoeae from patients attending Bangrak hospital (National Centre of Sexually Transmitted Infections), 67 isolates obtained during January-March 2000, 74 isolates obtained during January-March 2002, and 66 isolates obtained during October-December 2002, were tested. All isolates were susceptible to ceftriaxone while 71.5% and 74.4% were resistant to penicillin and quinolone, respectively. The high level of ciprofloxacin resistance (MIC =4 mg/L) also increased from 13.4% during January-March 2000 to 25.8% during October-December 2002. Multiple resistance determinants commonly coexisted in a single isolate so that the level of resistance was increased. The incidence of double resistance determinants, penicillin and quinolone resistance, were significantly increased from 34.3% among isolates during January to March 2000 up to 77.3% among isolates during October to December 2002 (P 1.024 g/L). Several plasmid patterns have been identified and various patterns of the plasmid can be artificially transferred and maintained their expression in Escherichia coli transformants. Such evidences infer the high mobility of resistant genome among microorganisms in the region. Moreover, the significant increase in penicillin and quinolone resistance herein, indicates the selective pressure and the diversity of genomic distribution among N. gonorrhoeae in Thailand. Primers JDA (5-TAC TCA ATC GGT AAT TGG CTT C-3) and JDB (5-CCA TAT CAC CGT CGG TAC TG-3) have been designed from sequences of the Asia, the Africa, and the Toronto ß-lactamase plasmids. By using the JDA and the JDB as PCR primers, our data reveal the highest prevalence and a significantly increasing trend of the epidemic Africa type of genomic ß-lactamase

Pengaruh Respon Tentang Media Video Pembelajaran Terhadap Hasil Belajar Materi Larutan Elektrolit Non Elektrolit SMA Gelekat Lewo Boru Kecamatan Wulanggitang Kabupaten Flores Timur Tahun 2020/2021

Tujuan utama penelitian ini adalah (1) Mengetahui validitas media video pembelajaran materi larutan elektrolit dan non elektrolit. (2) Mengetahui respon peserta didik tentang video pembelajaran pada materi larutan elektrolit dan non elektrolit. (3) Mengetahui hasil belajar peserta didik yang menggunakan video pembelajaran pada materi larutan elektrolit dan non elektrolit. (4) Mengetahui hubungan respon peserta didik tentang media video pembelajaran terhadap hasil belajar peserta didik setelah menggunakan video pembelajaran pada materi larutan elektrolit dan non elektrolit. (5) Mengetahui pengaruh respon peserta didik tentang media video pembelajaran terhadap hasil belajar peserta didik setelah menggunakan video pembelajaran pada materi larutan elektrolit dan non elektrolit. Berdasarkan hasil analisis data dan pembahasan dapat disimpulkan bahwa (1) Media video pembelajaran dinyatakan valid dengan nilai validitasnya sebesar 92,5.(2)Respon peserta didik kelas X IPA SMA Gelekat Lewo Boru Tahun Ajaran 2020/2021 termasuk dalam kategori sangat baik dengan presentase rata-rata sebesar 83%.(3)Hasil belajar peserta didik kelas X IPA SMA Gelekat Lewo Boru Tahun Ajaran 2020/2021 dinyatakan tuntas dengan nilai rata-rata sebesar 85.(4)Ada hubungan yang signifikan antara respon dengan hasil belajar peserta didik kelas X IPA SMA Gelekat Lewo Boru Tahun Ajaran 2020/2021 dengan koefisien korelasi sebesar 0,55(5)Ada pengaruh yang signifikan antara respon dengan hasil belajar peserta didik kelas X IPA SMA Gelekat Lewo Boru Tahun Ajaran 2020/2021 dengan persamaan regresi sederhana sebesar  = 57,64+ 0,33x. &nbsp

Activities of thiotetrahydropyridines as antioxidant and antimicrobial agents

Tetrahydropyridines have been reported previously as important medicinal agents. The present study, thiotetrahydropyridines were prepared and tested for antioxidants (DPPH and SOD assays) and antimicrobials (agar dilution method). The results show that 1-acetyl-1,2,3,4- and 1,2,3,6-thiotetrahydropyridines 15a-b, 16, 17 and 18a are new antioxidants that scavenge superoxide and free radicals. Whereas the analogs 15a and 16 are novel antimicrobials. Significantly, 1-acetyl-2-(1-adamantylthio)-3,4-diacetoxy-1,2,3,4-tetrahydropyridine (15a) is the most potent compound that inhibits the growth of Streptococcus pyogenes and Moraxella catarrhalis with MIC of 32 µg/mL, of Corynebacterium diphtheriae NCTC 10356 and of Vibrio cholerae (MIC of 64 µg/mL). Remarkably, the analog 15a is the most potent antioxidant and antimicrobial agent. This finding reveals a new and unique group of 1-acetyl-1,2,3,4-thiotetrahydropyridines as interesting lead compound with potential to be further developed for medicinal applications

Retinol-binding protein 4 and its potential roles in hypercholesterolemia revealed by proteomics

Effects of hypercholesterolemia on alterations of serum proteins have not been fully elucidated. Herein, using two-dimensional gel electrophoresis (2-DE) in conjunction with LC-MS searching has successfully been carried out to investigate the change of protein expression profiles as consequences of raised blood cholesterol at different levels (normal group: total cholesterol < 200 mg/dL; borderline high group: total cholesterol 200-239 mg/dL; and high group: total cholesterol ≥ 240 mg/dL) (n = 45). Results revealed that down-regulation of retinol-binding protein 4 (RBP4) (-2.26 fold), transthyretin (-1.25 fold) and gelsolin (-1.47 fold) was observed in the high group. Meanwhile, the other proteins such as haptoglobin, complement factor B and CD5 antigen-like protein were upregulated upto +3.24, +1.96 and +2.04 fold, respectively. Confirmation by Western blotting revealed a significant reduction of RBP4 (approximately 50 %) in individual samples derived from the high group. Presumptive conclusion can be drawn that down-regulation of RBP4 might be attributable to the inflammation of adipocytes caused by the release of proinflammatory cytokines (e.g. tumor necrosis factor α and interleukin-1β) from adipose tissues. Moreover, the decrease of transthyretin might also be taken into accounts since it is known that the transthyretin usually forms complex with RBP4 to prevent glomerular filtration and excretion through the kidney. The suppressing effect on RBP4 should be potentiated by the increase of complement factor B and CD5 antigen-like protein, which rendered the adipose tissues to overwhelm the liberation of RBP4 to blood circulation by metabolic and inflammatory processes. Such inflammation could further modulate the induction of cytokine release (e.g. IL-6 and IL-1β), resulting in the synthesis of acute phase protein, in particular, haptoglobin and Creactive proteins from hepatocytes. However, the mechanism of gelsolin reduction remains unclear. Among these differentially expressed proteins, the RBP4 has been proposed as a major linkage between hypercholesterolemia, adipose tissues, liver and kidney, which is believed to be a potential biomarker for metabolic and cardiovascular disorders associated with dyslipidemia in the future

EFEK PEMBERIAN MINUMAN SOPI DIBANDINGKAN ALKOHOL JENIS LAINNYA TERHADAP GAMBARAN HISTOPATOLOGI HATI TIKUS PUTIH (Rattus norvegicus) GALUR Sprague dawley

Sopi merupakan&nbsp; minuman alkohol hasil fermentasi dari nira atau sadapan lontar. Penelitian menunjukan bahwa mengkonsumsi alkohol 80 g perhari selama beberapa tahun dapat menyebabkan terjadinya hepatitis alkoholik dan dapat menyebabkan terjadinya sirosis hepatis. Selain itu&nbsp; hasil penelitian juga menunjukan bahwa orang dengan sirosis hepatis yang gagal berhenti mengkonsumsi alkohol memilki kurang dari 50% kesempatan hidup selama 5 tahun. Tujuan penelitian ini untuk menganalisis dan membandingkan gambaran mikroskopis hati antara Sopi dibandingkan dengan Vodka dan Bir. Metode penelitian ini merupakan penelitian eksperimental laboratorik dengan rancangan post-test controlled group design dengan menggunakan 24 ekor tikus yang dibagi menjadi 4 kelompok. K merupakan kelompok kontrol yang hanya diberi aquades. P1,P2 dan P3 merupakan kelompok eksperimental yang diberi Bir (4,7%), Vodka (40%) dan Sopi (53%) dengan dosis 8ml/kgBB selama 10 hari. Perubahan diamati secara mikroskopis dengan menggunakan kriteria Manja Roenigk yang dibagi menjadi 4 skor: sel normal, degenerasi parenkimatosa, degenerasi hidropik dan nekrosis. Data hasil penelitian diuji menggunakan uji statistik yaitu uji parametrik ANOVA dan uji Post Hoc LSD. Hasil pada kelompok P1 beberapa sel mengalami degenerasi parenkimatosa dengan nilai rata-rata skor 1,3 sedangkan kelompok P2 dan P3 beberapa sel mengalami degenerasi hidropik dengan nilai rata-rata skor 2,43. Hasil uji ANOVA didapatkan&nbsp; perbedaan bermakna (p=0,000). Hasil uji LSD didapatkan perbedaan bermakna antara K-P1 (p=0,000) K-P2 (p=0,000) K-P3 (p=0,000) P1-P2 (p=0,000) P1-P3 (p=0,000). Kesimpulan dari penelitian ini &nbsp;terdapat perbandingan gambaran mikroskopis hati yang bermakna antara pemberian Sopi dibandingkan Bir. Namun tidak terdapat perbandingan gambaran mikroskopis hati yang bermakna antara pemberian Sopi dibandingkan Vodk

PENINGKATAN PEMAHAMAN TERHADAP ILMU KIMIA MELALUI KEGIATAN PRAKTIKUM KIMIA SEDERHANA DI KOTA SOE

This community service was carried out on two topiks, there are a simple chemical practicum and the socialization of the risk of Plastik waste in Soe City, East Nusa Tenggara Province. This activity aims to increase the people's understanding of the chemical science which is considered dangerous for the organism. The mistake in understanding of chemical concept causes the chemistry science is to difficult to study, even though chemistry has comprehensive  benefits in life, such as in agriculture for fertilizer production, in pharmacy to synthesis the medicine, for culinary to nutrient content estimated, in the livestock sector to make vaccines, in the marine sector to manage the marine product and for environment to overcome the environmental pollution due to the waste. This activity was initiated by the students and lecturers in the Chemical Education Study Program, of Widya Mandira Catholic University with the target being Junior High School students in the Soe city. Simple practicum is done using natural materials around in order to create an easy chemistry lesson. In addition to practicum activities, there are also has a socialization activities about the risk  of Plastik waste, because Plastik waste is an environment problem in the future

Roles of kininogen-1, basement membrane specific heparan sulfate proteoglycan core protein, and roundabout homolog 4 as potential urinary protein biomarkers in diabetic nephropathy

Diabetic nephropathy, a major complication of diabetes mellitus (DM), is increasing worldwide and the large majority of patients have type 2 DM. Microalbuminuria has been used as a diagnostic marker of diabetic nephro­pathy. But owing to its insufficient sensitivity and specificity, other biomarkers are being sought. In addition, the pathophysiology of diabetic nephropathy is not fully understood and declines in renal function occur even without microalbuminuria. In this study, we investigated urinary proteins from three study groups (controls, and type 2 diabetic subjects with or without microalbuminuria). Non-targeted label-free Nano-LC QTOF analysis was conducted to discover underlying mechanisms and protein networks, and targeted label-free Nano-LC QTOF with SWATH was performed to qualify discovered protein candidates. Twenty-eight proteins were identified as candidates and functionally analyzed via String DB, gene ontology and pathway analysis. Four predictive mechanisms were analyzed: i) response to stimulus, ii) platelet activation, signaling and aggregation, iii) ECM-receptor interaction, and iv) angiogenesis. These mechanisms can provoke kidney dysfunction in type 2 diabetic patients via endothelial cell damage and glomerulus structural alteration. Based on these analyses, three proteins (kininogen-1, basement membrane-specific heparan sulfate proteoglycan core protein, and roundabout homolog 4) were proposed for further study as potential biomarkers. Our findings provide insights that may improve methods for both prevention and diagnosis of diabetic nephropathy

Pigmen Umbi Ubi Jalar Ungu dan Rimpang Kunyit: Indikator Ramah Lingkungan dan Murah untuk Eksperimen Titrasi Asam-Basa

[Pigments of Purple Sweet Potato and Turmeric Rhizome: Eco-Friendly and Cheap Indicators for Acid-Base Titration Experiment] Tujuan dari penelitian ini adalah mengkaji karakter pigmen umbi ubi jalar ungu (PUUJU) dan pigmen rimpang kunyit (PRK) pada larutan uji dan penggunaannya sebagai indikator titrasi asam basa dibandingkan dengan indikator fenolftalein. PRK diekstraksi dengan pelarut etanol sedangkan PUUJU diekstraksi dengan pelarut etanol dalam suasana asam. PUUJU dan PRK diuji karakter perubahan warnanya dan digunakan sebagai indikator titrasi HCl-NaOH dan CH3COOH-NaOH. Hasil penelitian menunjukkan PUUJU berwarna merah pada pH 1, pink pada pH 2-6, ungu pada pH 7, biru pada pH 8-9, hijau pada pH 10-11 dan kuning pada pH 12-14. PRK menunjukkan warna kuning pada pH 1-7, merah bata pudar pada pH 8 dan semakin pekat seiring naiknya nilai pH larutan. Kedua pigmen ini mampu menunjukkan perubahan warna yang jelas pada titik akhir titrasi layaknya indikator fenolftalien. Diperoleh konsentrasi HCl pada titrasi menggunakan indikator PP, PUUJU dan PRK berturut-turut adalah 0,1031 M, 0,1045 M dan 0,1025 M sedangkan konsentrasi CH3COOH berturut-turut adalah 0,0876 M, 0,0869 M dan 0,0853 M. Berdasarkan data-data ini, PUUJU dan PRK dapat digunakan sebagai indikator titrasi HCl-NaOH dan CH3COOOH-NaOH dalam praktikum kimia di kelas. Kata kunci/keyword: Volumetri, pigmen alami, ubi jalar ungu, kunyit, volumetry, natural pigment, purple sweet potato, turmeric rhizome