Influence of turbulence on the dynamo threshold

We use direct and stochastic numerical simulations of the magnetohydrodynamic equations to explore the influence of turbulence on the dynamo threshold. In the spirit of the Kraichnan-Kazantsev model, we model the turbulence by a noise, with given amplitude, injection scale and correlation time. The addition of a stochastic noise to the mean velocity significantly alters the dynamo threshold. When the noise is at small (resp. large) scale, the dynamo threshold is decreased (resp. increased). For a large scale noise, a finite correlation time reinforces this effect

Characteristics of pedaling muscle stiffness among cyclists of different performance levels

Background and Objectives: The aim of the present study was to compare the impact of an incremental exercise test on muscle stiffness in the rectus femoris (RF), vastus lateralis (VL), biceps femoris (BF), and gastrocnemius (GL) among road cyclists of three performance levels. Materials and Methods: The study group consisted of 35 cyclists grouped according to their performance level; elite (n = 10; professional license), sub-elite (n = 12; amateur license), and recreational (n = 13; cyclosportive license). Passive muscle stiffness was assessed using myometry before and after an incremental exercise test. Results: There was a significant correlation between time and category in the vastus lateralis with stiffness increases in the sub-elite (p = 0.001, Cohen’s d = 0.88) and elite groups (p = 0.003, Cohen’s d = 0.72), but not in the recreational group (p = 0.085). Stiffness increased over time in the knee extensors (RF, p < 0.001; VL, p < 0.001), but no changes were observed in the knee flexors (GL, p = 0.63, BF, p = 0.052). There were no baseline differences among the categories in any muscle. Conclusions: Although the performance level affected VL stiffness after an incremental exercise test, no differences in passive stiffness were observed among the main muscles implicated in pedaling in a resting state. Future research should assess whether this marker could be used to differentiate cyclists of varying fitness levels and its potential applicability for the monitoring of training load. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Étude expérimentale des transferts d'eau provoqués par l'irrigation sur une parcelle en moyenne vallée du fleuve Sénégal

Alors que l'irrigation paraît être le recours essentiel face aux besoins croissants de la population mondiale en céréales, la dégradation des sols et des eaux annihile presque totalement les efforts d'aménagement. Aussi l'étude de la dégradation des sols dans la moyenne vallée du fleuve Sénégal constitue l'un des quatre objectifs du pôle de recherches sur les systèmes irrigués sahéliens qui regroupe quatre pays dont le Mali, la Mauritanie, le Niger et le Sénégal. Le suivi de l'évolution des eaux et des sols sous culture apparaît indispensable pour garantir une agriculture irriguée rentable et durable dans la zone. Dans cette région, les risques de dégradation des sols font suite à l'effet de deux processus de dégradation que sont l'alcalinisation et la salinisation des sols. Ces types de dégradation sont fortement corrélés à la remontée des nappes d'eaux souterraines. Dans cet article, l'étude des transferts d'eau dans les parcelles irriguées par le biais d'un suivi expérimental " in situ " met en évidence les relations existantes entre les eaux d'irrigation et les mouvements de la nappe. L'étude a permis une meilleure compréhension de la dynamique hydrique au sein de la parcelle : saturation du profil et processus de recharge de la nappe. Elle a également permis de montrer que les échanges avec la nappe pendant la période d'irrigation sont négligeables. Cette pratique de la riziculture inondée, entraîne une variation de stock importante qui représente plus de 40% des apports et qui se traduit par une remontée de nappe de près de 2 mètres.Whereas irrigation appears to be the main approach to satisfy the growing worldwide demand for cereal, soil and water degradation continues to be an on-going problem in agriculture development. One of the main four concerns of the regional group for research on Sahelian irrigated systems (including Mali, Mauritania, Niger and Senegal) is soil degradation in the middle Senegal River valley. These soils are subject to various forms of degradation, mainly from salinisation and/or alkalinisation. These degradation processes are strongly correlated with water table dynamics, with water level fluctuations being significant. Therefore, to guarantee sustainable development of irrigated agriculture in the area, irrigation must be coupled with complete and permanent monitoring of soil and water quality.In this paper, we present a complete study concerning water transfer in irrigated plots and its effects on the groundwater table. The experimental site is located in the Podor region, at 16°.37'N, 14°.52'W in the Donaye irrigated area. The surface area is about 50 ha. Water supply is assured by filling a main channel using a group of pumps on the Doué River. Irrigation of the parcels is performed with siphons from this channel. The experimental plot of 0.33 ha is used for an underwater rice crop. One or two rice crop production harvests are made every year, with the decision been taken by the farmers. There is no drainage system in the area.The experimental plot was equipped with 8 piezometers located along a stream line. One is situated between the plot and the river in order to study the water movements caused by the exchange between the groundwater and the water in the river. Three are situated in the plot, one close to the Doué River, one in the middle of the plot and one near a dam, which is the opposite boundary compared to the river. Four other piezometers are located beyond the dam to estimate groundwater input and output at this boundary. Five tensiometers at 20, 40, 60, 95 and 135 cm depth are placed close to the piezometers located in the plot. Four water content profiles were measured during the irrigation period at depths 20, 40, 60 95 and 135 cm and the irrigation was performed over 84 days.The water table level variations at 2 meters were recorded. The groundwater inflow decreased during the first part of the irrigation period due to infiltration below the irrigated experimental plot. This inflow increased during a second period due to water level variations in the river close to this site. At the output, the head gradient did not vary appreciably and the outflow was assumed to be constant during the observation period. Moreover, this gradient is quite low and the flow rate is very low. The water content and pressure profiles clearly show the infiltration of water in the soil during irrigation. The head gradients show the water movement in the unsaturated soil during infiltration and evaporation. Upward flow due to evaporation is observed at about 10 days after irrigation. The last measured water profile (76 days after the end of irrigation) shows that evaporation modifies the water content profile until at least a depth of 120 cm.The water balance during the irrigation period showed that the input due to irrigation and precipitation was equal to 4150 m3. The evapotranspiration output was estimated to be 2370 m3. Groundwater exchange at the downstream boundary can be neglected during the duration of irrigation (84 days), since the average hydraulic gradient remained low (less than 0.8 %) and the hydraulic conductivity of the aquifer was not important (about 250 cm/day). It was assumed that the water storage quantified with the rise of the groundwater level was equal to the difference between surface input and evapotranspiration (more than 40 % of the contributions), leading to a 27 % change in water content, which is quite reasonable for this type of soil (clay).The stored water was then recovered by evapotranspiration and groundwater outflow at the plot boundaries. We are away from a reasonable irrigation that would reduce the used water quantity and decrease the risk of soil degradation. This study allows a better understanding of the water dynamics in the experimental plot, which includes soil saturation, recharge processes, and exchanges between the aquifer and the river

Effect of antimicrobial use on the resistance of Escherichia coli in faecal flora of pigs

The antimicrobial use in veterinary medicine is of concern because of possible transmisston of resistant bacteria to humans. However the relation between use and occurrence of resistance is poorly documented in the field. Sixteen farrow-to-fimsh herds were selected and classified on the frequency of antimicrobial administrations (low (LU), medium (MU) and high (HU) users). lndtcative Eschenchia coli strains were tsolated from faeces of sows (5 per herd) and young pigs (3 per sow) at several ttmes during animals\u27 hfe and tested for reststance to amoxicillin, gentamicin, trimethoprim-sulfamids and tetracyclin. The percentages of resistant strams were compared between herd groups

Characterization of microbulk detectors in argon- and neon-based mixtures

A recent Micromegas manufacturing technique, so called Microbulk, has been developed, improving the uniformity and stability of this kind of detectors. Excellent energy resolutions have been obtained, reaching values as low as 11% FWHM at 5.9 keV in Ar+5%iC4H10. This detector has other advantages like its flexible structure, low material budget and high radio-purity. Two microbulk detectors with gaps of 50 and 25 um have been characterized in argon- and neon-based mixtures with ethane, isobutane and cyclohexane. The results will be presented and discussed. The gain curves have been fitted to the Rose-Korff gain model and dependences of the electron mean free path and the threshold energy for ionization have been obtained. The possible relation between these two parameters and the energy resolution will be also discussed.Comment: Submitted to the Journal of Instrumentatio

Autologous skeletal muscle derived cells expressing a novel functional dystrophin provide a potential therapy for Duchenne Muscular Dystrophy

Autologous stem cells that have been genetically modified to express dystrophin are a possible means of treating Duchenne Muscular Dystrophy (DMD). To maximize the therapeutic effect, dystrophin construct needs to contain as many functional motifs as possible, within the packaging capacity of the viral vector. Existing dystrophin constructs used for transduction of muscle stem cells do not contain the nNOS binding site, an important functional motif within the dystrophin gene. In this proof-of-concept study, using stem cells derived from skeletal muscle of a DMD patient (mdcs) transplanted into an immunodeficient mouse model of DMD, we report that two novel dystrophin constructs, C1 (ΔR3-R13) and C2 (ΔH2-R23), can be lentivirally transduced into mdcs and produce dystrophin. These dystrophin proteins were functional in vivo, as members of the dystrophin glycoprotein complex were restored in muscle fibres containing donor-derived dystrophin. In muscle fibres derived from cells that had been transduced with construct C1, the largest dystrophin construct packaged into a lentiviral system, nNOS was restored. The combination of autologous stem cells and a lentivirus expressing a novel dystrophin construct which optimally restores proteins of the dystrophin glycoprotein complex may have therapeutic application for all DMD patients, regardless of their dystrophin mutation

Relationship Between Jump Capacity and Performance in BMX Cycling

The objective of this study is to assess the relationship between the results obtained on different vertical jump tests and the top score recorded during a BMX (Bicycle Moto-Cross) test and the rider''s performance. To do so, 10 BMX pilots participated in this study; 5 regarded as the elite group (EG) (age: 18.8 +/- 3.7, weight: 68.4 +/- 8.5 kg, height: 174 +/- 9 cm and previous BMX experience: 8 +/- 3.8 years) and 5 regarded as the recreational group (RG) (age: 19.8 +/- 4.8, weight: 69.2 +/- 11.7 kg, height: 170 +/- 9 cm, previous BMX experience: 4.2 +/- 1.3 years). Vertical jump capacity was obtained using the Bosco protocol, i.e. vertical squat jump (SJ), vertical countermovement jump (CMJ), drop jump (DJ) and repetitive jump (RJ), and time in race in a BMX circuit was determined. The results indicate a direct relationship between the time used to complete the circuit and the height of the jump reached in SJ (r: -.801; p:.017), CMJ (r : -.798; p :.018) and DJ (r : -.782; p:.022). This all suggests that assessing jump capacity using the Bosco test may be a useful tool for assessing BMX performance

Gap, a mycobacterial specific integral membrane protein, is required for glycolipid transport to the cell surface

The cell envelope of mycobacteria is a complex multilaminar structure that protects the cell from stresses encountered in the environment, and plays an important role against the bactericidal activity of immune system cells. The outermost layer of the mycobacterial envelope typically contains species-specific glycolipids. Depending on the mycobacterial species, the major glycolipid localized at the surface can be either a phenolglycolipid or a peptidoglycolipid (GPL). Currently, the mechanism of how these glycolipids are addressed to the cell surface is not understood. In this study, by using a transposon library of Mycobacterium smegmatis and a simple dye assay, six genes involved in GPLs synthesis have been characterized. All of these genes are clustered in a single genomic region of approximately 60 kb. We show by biochemical analyses that two non-ribosomal peptide synthetases, a polyketide synthase, a methyltransferase and a member of the MmpL family are required for the biosynthesis of the GPLs backbone. Furthermore, we demonstrate that a small integral membrane protein of 272 amino acids named Gap (gap: GPL addressing protein) is specifically required for the transport of the GPLs to the cell surface. This protein is predicted to contain six transmembrane segments and possesses homologues across the mycobacterial genus, thus delineating a new protein family. This Gap family represents a new paradigm for the transport of small molecules across the mycobacterial envelope, a critical determinant of mycobacterial virulence

Evolutionary history of tuberculosis shaped by conserved mutations in the PhoPR virulence regulator

Although the bovine tuberculosis (TB) agent, Mycobacterium bovis, may infect humans and cause disease, long-term epidemiological data indicate that humans represent a spill-over host in which infection with M. bovis is not self-maintaining. Indeed, human-to-human transmission of M. bovis strains and other members of the animal lineage of the tubercle bacilli is very rare. Here, we report on three mutations affecting the two-component virulence regulation system PhoP/PhoR (PhoPR) in M. bovis and in the closely linked Mycobacterium africanum lineage 6 (L6) that likely account for this discrepancy. Genetic transfer of these mutations into the human TB agent, Mycobacterium tuberculosis, resulted in down-regulation of the PhoP regulon, with loss of biologically active lipids, reduced secretion of the 6-kDa early antigenic target (ESAT-6), and lower virulence. Remarkably, the deleterious effects of the phoPR mutations were partly compensated by a deletion, specific to the animal-adapted and M. africanum L6 lineages, that restores ESAT-6 secretion by a PhoPR-independent mechanism. Similarly, we also observed that insertion of an IS6110 element upstream of the phoPR locus may completely revert the phoPR-bovis–associated fitness loss, which is the case for an exceptional M. bovis human outbreak strain from Spain. Our findings ultimately explain the long-term epidemiological data, suggesting that M. bovis and related phoPR-mutated strains pose a lower risk for progression to overt human TB, with major impact on the evolutionary history of TB