Mosaic fungal individuals have the potential to evolve within a single generation

Although cells of mushroom-producing fungi typically contain paired haploid nuclei (n + n), most Armillaria gallica vegetative cells are uninucleate. As vegetative nuclei are produced by fusions of paired haploid nuclei, they are thought to be diploid (2n). Here we report finding haploid vegetative nuclei in A. gallica at multiple sites in southeastern Massachusetts, USA. Sequencing multiple clones of a single-copy gene isolated from single hyphal filaments revealed nuclear heterogeneity both among and within hyphae. Cytoplasmic bridges connected hyphae in field-collected and cultured samples, and we propose nuclear migration through bridges maintains this nuclear heterogeneity. Growth studies demonstrate among- and within-hypha phenotypic variation for growth in response to gallic acid, a plant-produced antifungal compound. The existence of both genetic and phenotypic variation within vegetative hyphae suggests that fungal individuals have the potential to evolve within a single generation in response to environmental variation over time and space

Consequences Matter : Compassion in Conservation Means Caring for Individuals, Populations and Species

Funding This research received no external funding. Acknowledgments The manuscript benefitted from significant input from Dan Brockington, J.B. Callicott, Peter Coals, Tim Hodgetts, David Macdonald and Jeremy Wilson. Conflicts of Interest The authors declare no conflict of interest.Peer reviewedPublisher PD

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Acquired brain injury, parenting, social work, and rehabilitation: supporting parents to support their children

Growing numbers of adults live with the consequences of acquired brain injury (ABI). Those affected frequently require medical input, rehabilitation, and social care. Individuals could suffer from a range of impairments that affect functional abilities. Limited attention has been paid to parenting with an ABI both within the social work and ABI literature. Parents with ABI present specific challenges to social workers and rehabilitationists. Case studies are used to illustrate how services can work to protect and support all parties, facilitating engagement with rehabilitation. The article concludes by considering the knowledge needed to facilitate engagement with rehabilitation and support

Is career advancement important to disadvantaged jobseekers?

For many jobseekers low-paid work is in itself not a good stepping stone towards a better job, so the policy focus has thus shifted from helping welfare recipients to obtain jobs, to supporting employment in quality jobs that offer opportunities for wage and career progression. This paper looks at career aspirations among Australians who have experienced long periods out of the workforce. While much has been learned about what factors are likely to encourage or act as a barrier to career advancement, there has been comparatively less inquiry into how low-wage workers themselves define advancement and their attitudes towards it.his study seeks to fill part of the gap on research in this area using survey and qualitative interview data from the joint Melbourne Institute/ Brotherhood of St Laurence ARC Linkage project ‘Job retention and advancement of disadvantaged jobseekers’

Wars over Wildlife:Green Militarisation and Just War Theory

Militarisation of conservation (sometimes known as 'green militarisation') is an issue of growing international interest. Rhino horn is immensely valuable (in 2013 its value exceeded that of gold or cocaine), and its illegal trade has attracted widespread attention. Conservationists have declared a 'war' on poaching, with extensive military resources deployed to combat it. This sometimes includes operations which are referred to, particularly in the media, as 'shoot-to-kill'. These can be tantamount to extra-judicial killings. We scrutinise this issue using 'Just War' principles, to explore whether the 'war' on poaching meets the criteria expected of armed conflict. Our perspective suggests that it fails both ethical and pragmatic examination. This piece encourages conservation scientists, and the public, to consider which actions are justified in protecting wildlife, and how we should rethink conservation policy to achieve ethical, successful outcomes for both people and wildlife