1 research outputs found
Separation of Isomeric Forms of Urolithin Glucuronides Using Supercritical Fluid Chromatography
Urolithins are gut microbiota metabolites produced in
humans after
consuming foods containing ellagitannins and ellagic acid. Three urolithin
metabotypes have been reported for different individuals depending
on the final urolithins produced. After absorption, they are conjugated
with glucuronic acid (phase II metabolism), and these are the main
circulating metabolites in plasma and reach different tissues. Different
regioisomeric isomers of urolithin glucuronides have been described.
Still, their identification and quantification in humans have not
been properly reported due to resolution limitations in their analysis
by reversed-phase high-performance liquid chromatography. In the present
study, we report a novel method for separating these isomers using
supercritical fluid chromatography. With this method, urolithin A
3- and 8-glucuronide, isourolithin A 3- and 9- glucuronide, and urolithin
B 3-glucuronide (8-hydroxy urolithin 3-glucuronide; 3-hydroxy urolithin
8-glucuronide; 3-hydroxyurolithin 9-glucuronide; 9-hydroxyurolithin
3-glucuronide; and urolithin 3-glucuronide) were separated in less
than 15 min. The proposed method was applied to successfully analyze
these metabolites in urine samples from different volunteers belonging
to different metabotypes