Rôle des anticorps neutralisants autologues dans la guérison spontanée lors d'une infection par le virus de l'hépatite C

Environ 30% des patients infectés par le virus de l hépatite C guérissent spontanément. Le but de ce travailétait (i) d examiner l évolution des protéines d enveloppe en cas de guérison spontanée (ii) de comparerl infectivité des variants présents aux stades précoces (iii) d explorer la capacité neutralisante des anticorps(Ac) vis-à-vis des variants majoritaires et minoritaires. Nous avons sélectionné 2 patients avec une hépatiteaigue C suivie d une guérison très rapide. Pour explorer la capacité d entrée de ces variants et leur aptitude àêtre neutralisés, nous avons produit des pseudo-particules rétrovirales portant les enveloppes de différentsvariants. Pour le 1er patient, une réponse neutralisante autologue était détectable précocement, avec unmaximum entre le 2ème et le 3èmemois suivant la cytolyse. Elle était encore détectable au 30ème mois. Pourle 2ème patient, des Ac vis-à-vis du variant majoritaire étaient détectés dans le sérum prélevé 4 jours après lacytolyse et dans les sérums plus tardifs. Le titre des Ac était maximum au 5ème mois. La réponse neutralisanteest d apparition précoce et persiste même après l élimination virale. Ces observations nous questionnent parrapport au rôle éventuel joué par ces Ac dans les cas de recontamination.Only 30% of Hepatitis C virus infected individuals recover spontaneously. We investigated the mechanismsleading to early HCV clearance. The purpose of this work was: (i) to explore the diversity and the early geneticevolution of the HCV envelope glycoproteins, and the infectivity spectrum of isolated variants and (ii) toanalyze the ability of the autologous neutralizing response to control these variants. We selected two patientswho developed an acute HCV infection. To explore the impact of mutations on infectivity and neutralization,retroviral pseudoparticules were produced with representative E1 and E2 sequences. For the first case, themaximum neutralizing activity was observed in the serum collected between 2 and 3 months post ALT peak,the activity was still detectable after 30 months. For the second case, autologous neutralizing activity wasdetected in every serum collected between 4 days and 13 months after. A gradual increase of neutralizationactivity was observed over time with a maximum 5 to 6 months. We have shown that the neutralizing responsewas detectable at early stages of primoinfection and was sustained beyond the time at which the virus wascleared. These observations raise interesting questions about the role of such antibodies in case of re-exposure.TOURS-Bibl.électronique (372610011) / SudocSudocFranceF

Open-source games for health, multiplayer and gamepads. Co-creating fun care with children with asthma, young adults with cystic fibrosis, elders with COPD

Without air pollution, our life expectancy would increase by 34 months. With a commons-based economy, each and every human could afford medical care (vs one in two today). How not to produce risks? How to foster mutual care? We, people across regions and disciplines, get engaged to value pluralism and collective wisdom. We embrace play as a natural way to experiment, socialize, learn. We document and share our work so that everyone can freely use, study, improve, adapt it. In traditional medtech, corporate experts build a technical tool to manage a disease. Sealed in a black box, its price make it unaffordable to some. At Breathing Games, volunteer, diverse contributors co-create an evolving, rewarding, immersive story to promote holistic health. Free to use, study, improve, its gratuity helps develop solidarity. We present here our games (Asthma Heroes, Asthmonautes, Rise); controllers (Spirotroller enhanced, Spirotroller gaming, Breathing gamepad); creation as research (game testing, controller testing, games co-creation, translations); our next steps (international study, game accessibility via Raspberry Pi, link to air pollution sensor); and our advocacy activities for health as commons

Mutual care taking: collectively creating our respiratory wellbeing with open sciences

Background: Worldwide, 6 people out of 10 have no access to treatment, or are not encouraged to follow it. Air pollution alone kills 7 million people yearly, reduces our life expectancy by 20 months, and costs 6% the gross world product. Devices to assess lung capacity remain often unavailable in low / middle income countries. Actions: We co-create inclusive, open science knowledge: open source breath and air quality controllers, and libre / gratis education to reduce risks and make care fun. Learnings: Awareness: breath as a way to feel life, from childhood. Universal health: mutualizing resources to end poverty. Partnership: reducing barriers with remote participation

Feasibility of Antireflection and Passivation Coatings by Atmospheric Pressure PECVD

AbstractAn innovative method to obtain antireflection and passivation coatings on p-type crystalline silicon solar cells is presented. The method consists in the growth of hydrogenated silicon nitride films (SiNx:H) by atmospheric pressure plasma enhanced chemical vapor deposition (AP-PECVD). Compared to industrial process, using this kind of processing may allow an augmentation of the silicon solar cell production throughput and yield enabling a reduction of the cost of photovoltaic systems. The optical and passivation properties of the SiNx:H films grown by AP-PECVD are determined and compared with the standard low pressure plasma enhanced chemical vapor deposition (LP- PECVD) films. Minority carrier lifetime of 1ms was reached. Solar cells coated with AP-PECVD SiNx:H films were made, and their performances were compared with standard SiNx:H coated cells. The same cell efficiency is obtained for the two groups of cells proving the interest of the AP-PECVD for the solar cell industry

Zooplankton variability and climatic anomalies from 1994 to 2001 in the Balearic Sea (Western Mediterranean)

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