Phonological and orthographic processing in deaf readers during recognition of written and fingerspelled words in Spanish and English

The role of phonological and orthographic access during word recognition, as well as its developmental trajectory in deaf readers is still a matter of debate. This thesis examined how phonological and orthographic information is used during written and fingerspelled word recognition by three groups of deaf readers: 1) adult readers of English, 2) adult and 3) young readers of Spanish. I also investigated whether the size of the orthographic and phonological effects was related to reading skill and other related variables: vocabulary, phonological awareness, speechreading and fingerspelling abilities. A sandwich masked priming paradigm was used to assess automatic phonological (pseudohomophone priming; Experiments 1-3) and orthographic (transposed-letter priming; Experiments 4–6) effects in all groups during recognition of single written words. To examine fingerspelling processing, pseudohomophone (Experiments 7–9) and transposed-letter (Experiments 10-12) effects were examined in lexical decision tasks with fingerspelled video stimuli. Phonological priming effects were found for adult deaf readers of English. Interestingly, for deaf readers of Spanish only those young readers with a small vocabulary size showed phonological priming. Conversely, orthographic masked priming was found in adult deaf readers of English and Spanish as well as young deaf readers with large vocabulary size. Reading ability was only correlated to the orthographic priming effect (in accuracy) in the adult deaf readers of English. Fingerspelled pseudohomophones took longer than control pseudowords to reject as words in the adult deaf readers of English and in the young deaf readers of Spanish with a small vocabulary, suggesting sensitivity to speech phonology in these groups. The findings suggest greater reliance on phonology by less skilled deaf readers of both Spanish and English. Additionally, they suggest greater reliance on phonology during both word and fingerspelling processing in deaf readers of a language with a deeper orthography (English), than by expert readers of a shallow orthography (Spanish)

EVALUACIÓN DE LA RESPUESTA PRODUCTIVA, METABÓLICA Y DE LA CALIDAD DE LA CANAL Y DE LA CARNE DE OVINOS SUPLEMENTADOS CON CROMO ORGÁNICO

El objetivo fue evaluar dosis crecientes de levadura de cromo (LevCr) (T1=0 mg, T2=0.2 mg, T3=0.4 mg y T4=0.6 mg de Cr kg-1 MS) durante 50 días en la dieta de 24 ovinos machos enteros, de la raza Rambouillet (29.2±0.17 kg de PV) con 3.5 meses de edad. El diseño experimental fue completamente al azar con cuatro tratamientos (T) y 6 repeticiones por tratamiento. Se evaluó el crecimiento, concentración de triglicéridos, glucosa en plasma, contendido de Fe, Cu, Cr y Zn en hígado y hueso, contenido de ácidos grasos (AG), características de la canal y calidad de la carne. El crecimiento y las características de la canal no fueron afectados (P>0.05) por el Cr; en las semanas 7 y 11 hubo efecto cuadrático (P0.05) el contenido de Ca, P, Mg y Na en hígado o hueso, se observó una respuesta cuadrática (P<0.05) tanto en hígado como en hueso en el contendido de K, el contenido de Ca en hueso fue afectado (P<0.05) por el nivel de Cr en la dieta. El Cr redujo linealmente (P<0.01) los AG palmítico (C16:0) y esteárico (C18:0), y aumentó linealmente (P<0.01) linoleico (C18:2) y araquidónico (C20:4). Hubo efecto cuadrático (P<0.05) en la grasa intramuscular (GIM) y lineal (P<0.05) en el colágeno por efecto del Cr. Se concluye que el Cr no afectó el crecimiento ni las características de la canal, redujo el contenido de Fe en hígado y hueso, y de Cu en hígado; el Cr afectó el perfil de AG, redujo la GIM y aumentó el colágeno, pero no afectó la terneza de la carne de los ovinos. Palabras clave: ovinos, cromo levadura, carne, crecimiento, ácidos grasos, metabolismo

Effects of dietary chromium-yeast level on growth performance, blood metabolites, meat traits and muscle fatty acids profile, and microminerals content in liver and bone of lambs

To assess the effect of dietary supplement levels of chromium-yeast (Cr-yeast) on growth performance, blood glucose and triglycerides, fatty acid (FA) profile in intramuscular fat, carcase and meat traits, iron, copper, chromium and zinc concentrations in liver and bone, 24 Rambouillet male lambs (29.2 ± 0.17 kg body weight) were randomly assigned to four diets with 0, 0.2, 0.4 and 0.6mg Cr/kg DM. The growth performance trial lasted 49 d. Supplemental Cryeast did not affect growth performance and carcase characteristics (p>.05), but reduced (p<.05) perirenal and intramuscular fat, as well as 3 h post-feeding blood glucose and triglycerides concentration. In liver, Fe and Cu concentration decreased (p<.05), while Cr concentrations in liver increased with increasing Cr-yeast dietary levels. In bone, Fe decreased (p<.05) as Cryeast dietary levels increasing, and Cr-yeast supplementation increased Cr concentrations (p<.05). As Cr-yeast dietary level increased, palmitic (C16:0) and stearic (C18:0) SFA decreased linearly (p<.05), while palmitoleic (C16:1n-7), vaccenic (C18:1n-7), linoleic (C18:2n-6) and arachidic (C20:4) unsaturated fatty acids (UFA) increased linearly (p<.01). In conclusion, Cr-yeast did not affect growth performance and carcase quality, but decreased the perirenal and intramuscular fat, blood glucose and triglyceride content, and Fe and Cu concentrations in liver as increased Cr-yeast levels in the diet. Because supplemental Cr-yeast improved index of atherogenicity and unsaturated to saturated FA ratio in muscle of lambs, it could be of human nutritional interest

Effects of organic chromium supplementation to finishing lambs diet on growth performance, carcass characteristics and meat quality

The objective of this study was to evaluate supplemental organic chromium (Cr) to finishing lambs on growth performance, carcass characteristics, and meat quality. Eighteen Suffolk lambs (age (4.5±0.2) mon; (25.8±3.6) kg body weight (BW)) were randomly assigned to three levels of supplemental organic Cr (0.0, 0.2 and 0.4 mg kg–1 dry matter (DM)) in a complete random design. Growth performance was evaluated for 70 d, and then lambs were slaughtered to study carcass characteristics and chemical composition of meat. Orthogonal contrasts were performed (contrast one-average level 0.2 ppm Cr vs. average level 0.4 ppm Cr; contrast two-level 0 vs. average levels (0.2+0.4) ppm Cr). Orthogonal polynomials were used to estimate the linear and quadratic effects of Cr concentrations. Growth and carcass performance were not affected by supplemental organic Cr. Muscle conformation and leg perimeter linearly increased (P<0.05) as organic Cr level increased in the diet. Kidney fat decreased linearly (P<0.05) as supplemental Cr increased. In Longissimus dorsi (LD), the ash content decreased linearly, and shear force (kg cm–2) increased (P<0.05) as organic Cr level increased in the diet. It is concluded that organic Cr did not affect growth performance, but it improved positively the muscle conformation, reduced kidney fat, whereas in LD there was an increment in shear force in finishing carcass lambs

Shifts in phytoplankton community structure modify bacterial production, abundance and community composition

In recent decades, the phytoplankton community in parts of the Baltic Sea has shifted from diatom dominance to co-occurrence of diatoms and dinoflagellates during the spring bloom. We investigated whether this shift affects bacterial production (BP), abundance and community composition (BCC). Two mesocosm experiments were carried out with water from the SW coast of Finland during the winters of 2012 and 2013. The water was collected before the onset of the spring bloom. Natural seawater was used as a control, and various inocula of diatom and dino flagellate cultures were used as treatments. After the phytoplankton bloom development, BP (thymidine: BPT; leucine: BPL) was significantly higher in the diatom treatments than in the controls and dinoflagellate treatments (BPT and BPL in 2012 and BPL in 2013). In 2013, the BCC was significantly different between the diatom and dinoflagellate treatments and there was a temporal shift in both experiments. Alphaproteobacteria predominated in all treatments at the beginning of the experiments and shifted to flavobacterial (2012) and betaproteobacterial predominance (2013) during the chlorophyll a peak. Towards the end of the experiment, Actinobacteria and Betaproteo bacteria predominated in the diatom treatment in 2012, whereas in 2013 Flavobacteriia (all treatments) predominated together with Gammaproteobacteria and Cytophagia (diatom treatments). The results demonstrated that bacterial physiology and community structure are affected by relatively small changes in the phytoplankton community. Thus, the ongoing changes in the phytoplankton community resulting from co-occurrence of diatoms and dinoflagellates may decrease pelagic remineralization of carbon and reduce organic matter fluxes through the microbial loop.Peer reviewe

Partitioning the Heritability of Tourette Syndrome and Obsessive Compulsive Disorder Reveals Differences in Genetic Architecture

The direct estimation of heritability from genome-wide common variant data as implemented in the program Genome-wide Complex Trait Analysis (GCTA) has provided a means to quantify heritability attributable to all interrogated variants. We have quantified the variance in liability to disease explained by all SNPs for two phenotypically-related neurobehavioral disorders, obsessive-compulsive disorder (OCD) and Tourette Syndrome (TS), using GCTA. Our analysis yielded a heritability point estimate of 0.58 (se = 0.09, p = 5.64e-12) for TS, and 0.37 (se = 0.07, p = 1.5e-07) for OCD. In addition, we conducted multiple genomic partitioning analyses to identify genomic elements that concentrate this heritability. We examined genomic architectures of TS and OCD by chromosome, MAF bin, and functional annotations. In addition, we assessed heritability for early onset and adult onset OCD. Among other notable results, we found that SNPs with a minor allele frequency of less than 5% accounted for 21% of the TS heritability and 0% of the OCD heritability. Additionally, we identified a significant contribution to TS and OCD heritability by variants significantly associated with gene expression in two regions of the brain (parietal cortex and cerebellum) for which we had available expression quantitative trait loci (eQTLs). Finally we analyzed the genetic correlation between TS and OCD, revealing a genetic correlation of 0.41 (se = 0.15, p = 0.002). These results are very close to previous heritability estimates for TS and OCD based on twin and family studies, suggesting that very little, if any, heritability is truly missing (i.e., unassayed) from TS and OCD GWAS studies of common variation. The results also indicate that there is some genetic overlap between these two phenotypically-related neuropsychiatric disorders, but suggest that the two disorders have distinct genetic architectures