Spectral barcode label for fighting illegal waste dumps

Illegal waste dumping is one of the biggest problem in environment protection. Shipments of waste are left in wastelands, forests or dumped into the sea. These activities are difficult to suppress because technologies available to track the waste, and hence the people responsible, are very limited.(1) This research focuses on developing an effective, low-cost way of labelling and detecting bulk waste material shipments. The technology is based on an easily retrievable material in the form of micro/nano magnets which are used as carrier for the spectral signal transducers (e.g. dyes and pigments). These pigment carrying magnetic particles are chemically and physically stable, can form unique spectral pattern to use as identifier tag. The project is divided into two parts: (i) the modification of the magnet particles with various dyes and pigments to form spectral codes (ii) the development of analytical technique for code readout

Spectral barcode label for fighting illegal waste dumps

Illegal waste dumping is one of the biggest problem in environment protection. Shipments of waste are left in wastelands, forests or dumped into the sea. These activities are difficult to suppress because technologies available to track the waste, and hence the people responsible, are very limited.(1) This research focuses on developing an effective, low-cost way of labelling and detecting bulk waste material shipments. The technology is based on an easily retrievable material in the form of micro/nano magnets which are used as carrier for the spectral signal transducers (e.g. dyes and pigments). These pigment carrying magnetic particles are chemically and physically stable, can form unique spectral pattern to use as identifier tag. The project is divided into two parts: (i) the modification of the magnet particles with various dyes and pigments to form spectral codes (ii) the development of analytical technique for code readou

Photometric detection in flow analysis systems using integrated PEDDs

A novel inexpensive optical-sensing technique has been developed for colorimetric flow analysis. This sensing system employs two LEDs whereby one is used as the light source and the other as a light detector. The LED used as light detector is reverse biased with a 5-V supply so that the photocurrent generated by the incident light discharges the capacitance. Direct digital output is provided by a simple timer circuit that measures the time taken for this discharge process from 5 V (logic 1) to 1.7 V (logic 0). This sensing concept has been applied in flow analysis by constructing an optical flow cell with a pair of LEDs. Calibration of the integrated optical flow cell using a dye resulted in a linear response that obeys the Beer–Lambert law. The flow rate, dynamic range, sensitivity and limits of detection were investigated. The system was also used for pH determination in the range of pH 2.5–6.8 using bromocresol green (BCG). The pKa of BCG was successfully determined by this technique

anchors: Software for Anchoring Vignette Data

When respondents use the ordinal response categories of standard survey questions in different ways, the validity of analyses based on the resulting data can be biased. Anchoring vignettes is a survey design technique intended to correct for some of these problems. The anchors package in R includes methods for evaluating and choosing anchoring vignettes, and for analyzing the resulting data.

Size Control and Fluorescence Labeling of Polydopamine Melanin-Mimetic Nanoparticles for Intracellular Imaging.

As synthetic analogs of the natural pigment melanin, polydopamine nanoparticles (NPs) are under active investigation as non-toxic anticancer photothermal agents and as free radical scavenging therapeutics. By analogy to the widely adopted polydopamine coatings, polydopamine NPs offer the potential for facile aqueous synthesis and incorporation of (bio)functional groups under mild temperature and pH conditions. However, clear procedures for the convenient and reproducible control of critical NP properties such as particle diameter, surface charge, and loading with functional molecules have yet to be established. In this work, we have synthesized polydopamine-based melanin-mimetic nanoparticles (MMNPs) with finely controlled diameters spanning ≈25 to 120 nm and report on the pH-dependence of zeta potential, methodologies for PEGylation, and the incorporation of fluorescent organic molecules. A comprehensive suite of complementary techniques, including dynamic light scattering (DLS), cryogenic transmission electron microscopy (cryo-TEM), X-ray photoelectron spectroscopy (XPS), zeta-potential, ultraviolet-visible (UV-Vis) absorption and fluorescence spectroscopy, and confocal microscopy, was used to characterize the MMNPs and their properties. Our PEGylated MMNPs are highly stable in both phosphate-buffered saline (PBS) and in cell culture media and exhibit no cytotoxicity up to at least 100 μg mL-1 concentrations. We also show that a post-functionalization methodology for fluorophore loading is especially suitable for producing MMNPs with stable fluorescence and significantly narrower emission profiles than previous reports, suggesting they will be useful for multimodal cell imaging. Our results pave the way towards biomedical imaging and possibly drug delivery applications, as well as fundamental studies of MMNP size and surface chemistry dependent cellular interactions

Chemical event tracking using a low-cost wireless chemical sensing network

A recently developed low-cost light emitting diode (LED) chemical sensing technique is integrated with a Mica2Dot wireless communications platform to form a deployable wireless chemical event indicator network. The operation of the colorimetric sensing node has been evaluated to determine its reproducibility and limit of detection for an acidic airborne contaminant. A test-scale network of five similar chemical sensing nodes is deployed in a star communication topology at fixed points within a custom built Environmental Sensing Chamber (ESC). Presented data sets collected from the deployed wireless chemical sensor network (WCSN) show that during an acidic event scenario it is possible to track the plume speed and direction, and estimate the concentration of chemical plume by examining the collective sensor data relative to individual sensor node location within the monitored environment

Detection of nitrite by flow injection analysis using a novel paired emitter-detector diode (PEDD) as a photometric detector

An inexpensive flow injection analysis system for determining low concentration levels of nitrite employing the Griess reagent spectrophotometric method is reported. The novel photometric detector applied within this manifold is a highly sensitive, low cost, miniaturized light emitting diode (LED) based flow detector. This colorimetric detector employs two LEDs, operating one as a light source and the other as a light detector. The emitter LED is forward biased and the detector reverse biased. The emitter and detector LED had a λmax of 530 nm and 623 nm respectively. The emission spectrum of the emitter LED efficiently overlapped with the absorbance spectrum of 9 µM NO2 and Griess reagent complex. A simple timer circuit measures the time taken for the photocurrent generated by the emitter LED to discharge the detector LED from 5 V (logic 1) to 1.7 V (logic 0). The Griess reagent method employed for nitrite determination is based on the formation of an azo dye, the intensity of which is directly related to nitrite concentration. The linear range, reproducibility and limit of detection were investigated. Detection limits in the nanomolar range were achieved using the Paired Emitter-Detector Diode (PEDD) flow analysis device. For a comparative study the linear range and limit of detection were also investigated using a platewell reader. Higher sensitivity and improved precision were obtained from the PEDD compared to the commercially available plate well reader

Automatic reaction to a chemical event detected by a low-cost wireless chemical sensing network

A test-scale wireless chemical sensor network (WCSN) has been deployed within a controlled Environmental Chamber (EC). The combined signals from the WCSN were used to initiate a controllable response to the detected chemical event. When a particular sensor response pattern was obtained, a purging cycle was initiated. Sensor data were continuously checked against user-defined action limits, to determine if a chemical event had occurred. An acidic contaminant was used to demonstrate the response of the sensor network. Once the acid plume was simultaneously detected by a number of wireless chemical sensor nodes, an automatic response action, which was the purging of the EC with clean air, was initiated and maintained for a period of time until the WCSN indicated that normal status had been re-established

Wearable gas sensors

Wearable sensing applications have attracted much attention in recent years. The aim of the FP6 funded Proetex project is improving safety and efficiency of emergency personnel by developing integrated wearable sensor systems. This paper describes recent developments in the integration of sensing platforms into wearables for the continuous monitoring of environmentally harmful gases surrounding emergency personnel. Low-power miniature CO and CO2 sensors have been successfully integrated in a jacket collar and boot worn by emergency personnel. These sensors need to provide information about the level of gas in the surrounding environment without obstructing the activities of the wearer. This has been achieved by integrating special pockets on the jacket and boot of fire-fighters. Each sensor is attached to a sensing module for signal accommodation and data transfer. The sensor performance has been evaluated by simulation of real-life situations. These wearable gas sensors will dramatically improve personnel awareness of potential hazard and can function as a personal warning system. In this way, fire-fighter’s jacket and boot not only protect the wearer, but have a second function of providing valuable information on external hazards. The authors gratefully acknowledge the financial support of the Science Foundation Ireland (07/CE/I1147) and the EU project FP6-2004-IST-4-026987. We also acknowledge contribution of University of Pisa (Italy), Zarlink Semiconductor (UK), and Diadora/Invicta Group (Italy)

Development of optical sensing system for detection of Fe ions using conductive polymer actuator based microfluidic pump

In this paper, we present a novel microfluidic optical sensing system by combining a low-power conductive polymer -based microfluidic pump and a microfluidic chip integrated with an optical sensor. A self priming microfluidic pump is developed using a polypyrrole. A microfluidic chip- optical detector module that contained an optical cuvette with LED and photo-diode optical sensing module was fabricated. Integration of the micro pump and the microfluidic chips complete the sensing system. The pump performance and its application in chemical analysis have been demonstrated in the detection of Fe ions