Installation of insecticide-treated durable wall lining: evaluation of attachment materials and product durability under field conditions.

BACKGROUND: Insecticide-treated durable wall lining (DL) is a new method of vector control designed to supplement LLINs and overcome two inherent limitations of LLINs and IRS: nightly behavioural compliance and short residual activity, respectively. DL is a deltamethrin-treated polyethylene material, which when used to cover interior house walls, functions as long-lasting IRS. Because the DL concept anticipates minimal upkeep, a primary challenge is how to guarantee correct household installation and in situ longevity for several years. Field trials were undertaken on various wall surfaces in Ghana to identify a logistically feasible, durable and re-usable method for DL wall attachment and to pilot new methods for assessing DL durability. METHODS: Over fifty-five candidate attachment or fixing products, including mechanical fasteners, material anchors and adhesives, were evaluated for their ability to tolerate static loads (simulating long-term installation) and short-term heavy weights (imitating shock damage). Attachment products were also scored using qualitative logistical and feasibility criteria, including ease of preparation, grip of fixing to DL and possibility of re-use. RESULTS: The stress tests provided a standardised, reproducible and reliable system for assessing fixing effectiveness and DL durability, with 64% (14/22) of adhesives and 15% (2/13) of mechanical fasteners failing to meet the minimum requirements of attaching DL to mud walls for set time periods. For most fixings, less outward load (0.2-8.0 kg) was required to detach DL from the wall, compared to downward load (0.2-19.2 kg). Fixings were better able to grip DL onto concrete than clay surfaces. Using a plastic nail cap to increase DL attachment area greatly improved grip and outward load tolerance, more so than varying nail size, length or texture. CONCLUSIONS: Based on a series of systematic stress tests, optimized fixing products for polyethylene DL wall attachment were identified. In parallel, a detailed and adaptable method of DL household installation was developed for routine deployment in malaria endemic areas. These standardized stress tests will form the basis for comparative evaluations of new types of DL textile, which incorporate non-pyrethroid insecticides to control malaria transmitted by resistant mosquito populations

A Coupled Experimental and Computational Approach to Quantify Deleterious Hemodynamics, Vascular Alterations, and Mechanisms of Long-Term Morbidity in Response to Aortic Coarctati

Introduction Coarctation of the aorta (CoA) is associated with morbidity despite treatment. Although mechanisms remain elusive, abnormal hemodynamics and vascular biomechanics are implicated. We present a novel approach that facilitates quantification of coarctation-induced mechanical alterations and their impact on vascular structure and function, without genetic or confounding factors. Methods Rabbits underwent thoracic CoA at 10 weeks of age (~ 9 human years) to induce a 20 mm Hg blood pressure (BP) gradient using permanent or dissolvable suture thereby replicating untreated and corrected CoA. Computational fluid dynamics (CFD) was performed using imaging and BP data at 32 weeks to quantify velocity, strain and wall shear stress (WSS) for comparison to vascular structure and function as revealed by histology and myograph results. Results Systolic and mean BP was elevated in CoA compared to corrected and control rabbits leading to vascular thickening, disorganization and endothelial dysfunction proximally and distally. Corrected rabbits had less severe medial thickening, endothelial dysfunction, and stiffening limited to the proximal region despite 12 weeks of normal BP (~ 4 human years) after the suture dissolved. WSS was elevated distally for CoA rabbits, but reduced for corrected rabbits. Discussion These findings are consistent with alterations in humans. We are now poised to investigate mechanical contributions to mechanisms of morbidity in CoA using these methods

Spectroscopy of globular clusters in the low-luminosity spiral galaxy NGC 45

CONTEXT: Extragalactic globular clusters have been studied in elliptical galaxies and in a few luminous spiral galaxies, but little is known about globular clusters in low-luminosity spirals. AIMS: Past observations with the ACS have shown that NGC 45 hosts a large population of globular clusters (19), as well as several young star clusters. In this work we aim to confirm the bona fide globular cluster status for 8 of 19 globular cluster candidates and to derive metallicities, ages, and velocities. METHODS: VLT/FORS2 multislit spectroscopy in combination with the Lick/IDS system was used to derive velocities and to constrain metallicities and [alpha/Fe] element ratio of the globular clusters. RESULTS: We confirm the 8 globular clusters as bona fide globular clusters. Their velocities indicate halo or bulge-like kinematics, with little or no overall rotation. From absorption indices such as H_beta, H_gamma, and H_delta and the combined [MgFe]' index, we found that the globular clusters are metal-poor [Z/H]<=-0.33 dex and [alpha/Fe]<=0.0 element ratio. These results argue in favor of a population of globular clusters formed during the assembling of the galaxy.Comment: Accepted for publication in Astronomy and Astrophysics. 10 pages, 6 figures. Table 6 and Fig. 6 will only be published in the electronic edition of the A&A journa

A search for massive UCDs in the Centaurus Galaxy Cluster

We recently initiated a search for ultra-compact dwarf galaxies (UCDs) in the Centaurus galaxy cluster (Mieske et al. 2007), resulting in the discovery of 27 compact objects with -12.2<M_V<-10.9 mag. Our overall survey completeness was 15-20% within 120 kpc projected clustercentric distance. In order to better constrain the luminosity distribution of the brightest UCDs in Centaurus, we continue our search by substantially improving our survey completeness specifically in the regime M_V<-12 mag (V_0<21.3 mag). Using VIMOS at the VLT, we obtain low-resolution spectra of 400 compact objects with 19.3<V_0<21.3 mag (-14<M_V<-12 mag at the Centaurus distance) in the central 25' of the Centaurus cluster, which corresponds to a projected radius of ~150 kpc. Our survey yields complete area coverage within ~120 kpc. For 94% of the sources included in the masks we successfully measure a redshift. Due to incompleteness in the slit assignment, our final completeness in the area surveyed is 52%. Among our targets we find three new UCDs in the magnitude range -12.2<M_V<-12 mag, hence at the faint limit of our survey. One of them is covered by archival HST WFPC2 imaging, yielding a size estimate of r_h <= 8-9 pc. At 95% confidence we can reject the hypothesis that in the area surveyed there are more than 2 massive UCDs with M_V<-12.2 mag and r_eff <=70 pc. Our survey hence confirms the extreme rareness of massive UCDs. We find that the radial distributions of Centaurus and Fornax UCDs with respect to their host clusters' centers agree within the 2 sigma level.Comment: 9 pages, 7 figures, accepted as Research Note for A&

The ISSI international study team on the martian PBL – status report and plan

Dynamical processes in the Martian boundary layer provide the means of communication between surface ice deposits and the free atmosphere, and the means of lifting dust from the surface. The boundary layer is therefore one of the most important components of the Martian climate system. The Martian boundary layer differs from that of the Earth in that it is more strongly forced, it is deeper, and the relative importance of radiative and convective heat fluxes in the lower boundary layer can be quite different. In order to understand the Martian boundary layer, a combination of theoretical, modeling and observational studies are necessary. Interactions between theorists, modelers, and observational scientists are needed to make progress and to provide a basis for analysis of data expected from Phoenix, Mars Science Laboratory, ExoMars and other future landed missions (such as a surface network mission), or missions such as balloons or other aircraft operating in the neutral atmosphere. The prime goal of this project under the auspices of the International Space Science Institute (ISSI) is to review and assess the current knowledge and understanding of Martian planetary boundary layer and its interactions with the surface and free atmosphere. We aim to promote international communication and collaboration to enhance the rate of acquisition of knowledge and understanding. This will be achieved through an International Study Team and publication of overview papers and individual reports on recent advances in this area

The Age Difference between the Globular Cluster Sub-populations in NGC 4472

The age difference between the two main globular cluster sub-populations in the Virgo giant elliptical galaxy, NGC 4472 (M 49), has been determined using HST WFPC2 images in the F555W and F814W filters. Accurate photometry has been obtained for several hundred globular clusters in each of the two main sub-populations, down to more than one magnitude below the turn-over of their luminosity functions. This allows precise determinations of both the mean colors and the turn-over magnitudes of the two main sub-populations. By comparing the data with various population synthesis models, the age-metallicity pairs that fit both the observed colors and magnitudes have been identified. The metal-poor and the metal-rich globular clusters are found to be coeval within the errors ($\sim 3$ Gyr). If one accepts the validity of our assumptions, these errors are dominated by model uncertainties. A systematic error of up to 4 Gyr could affect this result if the blue and the red clusters have significantly different mass distributions. However, that one sub-population is half as old as the other is excluded at the 99% confidence level. The different globular cluster populations are assumed to trace the galaxy's major star-formation episodes. Consequently, the vast majority of globular clusters, and by implication the majority of stars, in NGC 4472 formed at high redshifts but by two distinct mechanisms or in two episodes.Comment: 32 pages, including 12 postscript figures, accepted for publication in the Astronomical Journal, December 1999 issu

Using exomarkers to assess mitochondrial reactive species in vivo

Background: The ability to measure the concentrations of small damaging and signalling molecules such as reactive oxygen species (ROS) in vivo is essential to understanding their biological roles. While a range of methods can be applied to in vitro systems, measuring the levels and relative changes in reactive species in vivo is challenging. Scope of review: One approach towards achieving this goal is the use of exomarkers. In this, exogenous probe compounds are administered to the intact organism and are then transformed by the reactive molecules in vivo to produce a diagnostic exomarker. The exomarker and the precursor probe can be analysed ex vivo to infer the identity and amounts of the reactive species present in vivo. This is akin to the measurement of biomarkers produced by the interaction of reactive species with endogenous biomolecules. Major conclusions and general significance: Our laboratories have developed mitochondria-targeted probes that generate exomarkers that can be analysed ex vivo by mass spectrometry to assess levels of reactive species within mitochondria in vivo. We have used one of these compounds, MitoB, to infer the levels of mitochondrial hydrogen peroxide within flies and mice. Here we describe the development of MitoB and expand on this example to discuss how better probes and exomarkers can be developed. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons and biophysics of membrane proteins. Guest Editor: Christine Winterbourn. Abbreviations: EPR, electron paramagnetic resonance; GFP, green fluorescent protein; 4-HNE, 4-hydroxynonenal; MitoB, 3-(dihydroxyboronyl)benzyltriphenylphosphonium bromide; MitoP, (3-hydroxybenzyl)triphenylphosphonium bromide; ROS, reactive oxygen species; SOD, superoxide dismutase; TPMP, methyltriphenylphosphonium; TPP, triphenylphosphonium catio