Avaliação da memória declarativa emocional em pacientes com epilepsia temporal mesial e pacientes submetidos à lobectomia temporal mesial

ABSTRACTEpileptic seizures generate cognitive and behavioral impacts in individuals who suffer from epilepsy. Declarative memory is one of the cognitive functions that can be affected by epileptic seizures. The main objective of this work was to investigate neurocognitive function, especially the emotional working memory of patients with unilateral mesial temporal lobe epilepsy, and that of patients submitted to unilateral mesial temporal lobectomy. A face recognition test that can simultaneously recruit the frontal lobe (working memory) and mesial temporal lobe (emotional memory) was used to investigate emotional working memory. Our findings showed that the epilepsy factor significantly compromised the performance in the emotional memory test. On the other hand, surgical removal of the epileptic focus promoted an improvement in the emotional working memory of these patients, in addition to the significantly decrease in the number of seizures. ________________________________________________________________________________ RESUMOCrises epilépticas geram impactos comportamentais e cognitivos em indivíduos que sofrem de epilepsia. Uma das funções cognitivas que pode ser afetada pelas crises epilépticas é a memória declarativa. O objetivo do nosso estudo foi investigar funções cognitivas, especialmente a memória operacional emocional de pacientes com epilepsia temporal mesial unilateral e pacientes submetidos a lobectomia temporal mesial unilateral. Para investigar a memória operacional emocional foi utilizado um teste de reconhecimento de faces que pode recrutar simultaneamente o lobo frontal (memória operacional) e o lobo temporal mesial (memória emocional). Nossos resultados demonstram que o fator epilepsia compromete de forma significativa o desempenho no teste de memória emocional. Por outro lado, a remoção cirúrgica do foco epiléptico promoveu uma melhora na memória emocional desses pacientes, além de diminuir o número de crises

15-Deoxy-Δ 12,14

The cyclopentenone prostaglandin 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) is a natural ligand of peroxisome proliferator-activated receptor gamma (PPAR-γ) and a potential mediator of apoptosis in cancer cells. In the present study, we evaluated the effect of 15d-PGJ2 in human thyroid papillary carcinoma cells (TPC-1) using different doses of 15d-PGJ2 (0.6 to 20 μM) to determine IC50 (9.3 μM) via the MTT assay. The supernatant culture medium of the TPC-1 cells that was treated either with 15d-PGJ2 or with vehicle (control) for 24 hours was assessed for IL-6 secretion via CBA assay. RT-qPCR was used to evaluate mRNA expression of IL-6, SOCS1, SOCS3, and STAT3. TPC-1 cells treated with 15d-PGJ2 decreased the secretion and expression of IL-6 and STAT3, while it increased SOCS1 and SOCS3. Overall, we demonstrated that 15d-PGJ2 downregulated IL-6 signaling pathway and led TPC-1 cells into apoptosis. In conclusion, 15d-PGJ2 shows the potential to become a new therapeutic approach for thyroid tumors

Sistemas de Gestión Bibliotecaria desarrollados en Latinoamérica I: La experiencia de Presys y EspaBiblio.

Interview to leaders projects Presys and EspaBiblio

Avaliação da memória operacional, emocional e espacial, em pacientes com epilepsia temporal mesial e pacientes submetidos a lobectomia temporal

Tese (doutorado)—Universidade de Brasília, Faculdade de Ciências da Saúde, 2009.As crises epiléticas podem gerar importantes impactos cognitivos e comportamentais nos indivíduos que sofrem de epilepsia. As estruturas do lobo temporal medial são vitais para aquisição e consolidação das memórias declarativas juntamente com áreas corticais adjacentes particularmente da região frontal; portanto, uma das funções cognitivas que pode ser afetada por crises epiléticas é a memória declarativa. Neste sentido, o objetivo principal de nosso trabalho foi investigar a função neurocognitiva, particularmente a memória operacional, emocional e espacial, de pacientes com epilepsia temporal medial unilateral e pacientes submetidos à lobectomia unilateral do lobo temporal medial. Para investigação da memória operacional emocional foi utilizado um teste de reconhecimento de faces que possui a capacidade de recrutar, simultaneamente, a participação do lobo frontal (memória operacional) e do lobo têmporo-medial (memória emocional) e para a investigação da memória operacional espacial foi utilizado o teste de reconhecimento espacial com retardo (TRER). Os dados encontrados demonstraram que o fator epilepsia comprometeu significantemente o desempenho nos testes de memória operacional emocional e espacial. Os dados sugerem, ainda, que a remoção cirúrgica do foco epilético promoveu uma melhora na memória operacional, emocional e espacial, destes pacientes, além de diminuir significativamente o número de crises. _____________________________________________________________________________________ ABSTRACTEpileptic seizures may have important cognitive and behavioral impacts on individuals who suffer from epilepsy. The structures of the medial temporal lobe together with adjacent cortical areas, particularly those in the frontal region, are vital for the acquisition and consolidation of declarative memories. Therefore, one of the cognitive functions that can be affected by seizures is declarative memory. In this sense, the main objective of our work was to investigate neurocognitive function, especially emotional and spatial working memories, of patients with unilateral mesial temporal lobe epilepsy and patients submitted to unilateral lobectomy of the medial temporal lobe. A facial recognition test capable of simultaneously recruiting the frontal (operational memory) and medial temporal lobes (emotional memory) was used to investigate emotional working memory and a spatial recognition test with delay was used to investigate spatial working memory. The results show that the factor epilepsy significantly compromised performance in emotional and spatial working memory tests. The results also show that surgical removal of the epileptic focus improved the emotional and spatial working memories of these patients in addition to significantly reducing the number of seizures

INVESTIGATION OF LUMO-PELVIC RHYTHM IN PEOPLE WITH HIP PAIN

BACKGROUND: Prior studies have shown that aberrant lumbopelvic coordination (LPC) patterns could indirectly suggest alterations in neuromuscular control of trunk motion as well as altered lumbar joint loading. The effects of low back pain (LBP) on LPC have been previously studied yet despite the high incidence of LBP in those with hip-related pain (60%), the combined impact of LBP and hip pain (i.e. spine hip syndrome) on LPC is not well understood. Thus, the main objective of this study is to assess the LPC during a lumbar flexion/extension task in subjects with no hip pain, hip pain and concomitant LBP and hip pain. It is expected that this study will provide insight into the LPC that is associated with combined hip pain and LBP and will aid in developing interventions to optimize LPC to reduce LBP in people with concomitant hip and low back pain. METHODS: The data used in this study will consist of adults with no prior history of spinal or lower extremity surgery, BMI \u3c 35 kg·m-2 and will be derived from our current on-going research studies. These adults will be divided into three groups: 1) asymptomatic, healthy controls 2) hip pain 3) LBP and hip pain. The presence of hip pain and LBP will be self-reported by each participant and the Hip disability and Osteoarthritis Outcome Score (HOOS) survey and the self-report Numeric Rating Scale will be used to assess severity of hip and low back pain, respectively. A 3D gait analysis was performed of each participant while performing seven continuous repetitions of trunk flexion and extension at a self-selected speed while keeping their legs extended. A custom written MATLAB algorithm will be developed and used to assess peak thoracic, pelvic, and lumbar angles, thoracic, pelvic and lumbar range of motion and the lumbar-thoracic ratio (peak thoracic - peak pelvic/peak thoracic) measure. An ANOVA, with necessary adjustments for covariates, will be used to assess between-group differences in biomechanical outcomes with α=0.05 and multiple testing corrections performed as needed. ANTICIPATED RESULTS: It is hypothesized that the participants with hip pain and concomitant hip pain and LBP will exhibit altered LPC patterns compared to the control group. These data will help clinicians understand the potential role of the lumbar spine in development of LBP in those with hip pain and aid in developing interventions to treat people with hip-spine syndrome

Efficient Detection of Proteins Retro-Translocated from the ER to the Cytosol by In Vivo Biotinylation

Retro-translocation from the ER to the cytosol of proteins within the secretory pathway takes place on misfolded molecules that are targeted for degradation by the cytosolically located 26S proteasome complex. Retro-translocation occurs also for other proteins (such as calreticulin) that, despite being synthesized and transported to the ER, are in part dislocated to the cytosol. We have taken advantage of the E. coli derived biotin-ligase (BirA) expressed in the cytosol of mammalian cells to specifically biotin-label in vivo proteins within the secretory pathway that undergo retro-translocation. We validated the method using four different proteins that are known to undergo retro-translocation upon different conditions: the human trans-membrane protein MHC class-I a chain (MHC-Ia), the Null Hong Kong mutant of the secretory a1 anti-trypsin (NHKa1AT), the immunoglobulin heavy chain (HC) and the ER chaperone calreticulin (Crt). We observed specific monobiotinylation of cytosolically dislocated molecules, resulting in a novel, reliable way of determining the extent of retrotranslocation

Emotional declarative memory assessment of patients with mesial temporal lobe epilepsy and patients submitted to mesial temporal lobectomy Avaliação da memória declarativa emocional em pacientes com epilepsia temporal mesial e pacientes submetidos à lobectomia temporal mesial

Epileptic seizures generate cognitive and behavioral impacts in individuals who suffer from epilepsy. Declarative memory is one of the cognitive functions that can be affected by epileptic seizures. The main objective of this work was to investigate neurocognitive function, especially the emotional working memory of patients with unilateral mesial temporal lobe epilepsy, and that of patients submitted to unilateral mesial temporal lobectomy. A face recognition test that can simultaneously recruit the frontal lobe (working memory) and mesial temporal lobe (emotional memory) was used to investigate emotional working memory. Our findings showed that the epilepsy factor significantly compromised the performance in the emotional memory test. On the other hand, surgical removal of the epileptic focus promoted an improvement in the emotional working memory of these patients, in addition to the significantly decrease in the number of seizures.Crises epilépticas geram impactos comportamentais e cognitivos em indivíduos que sofrem de epilepsia. Uma das funções cognitivas que pode ser afetada pelas crises epilépticas é a memória declarativa. O objetivo do nosso estudo foi investigar funções cognitivas, especialmente a memória operacional emocional de pacientes com epilepsia temporal mesial unilateral e pacientes submetidos a lobectomia temporal mesial unilateral. Para investigar a memória operacional emocional foi utilizado um teste de reconhecimento de faces que pode recrutar simultaneamente o lobo frontal (memória operacional) e o lobo temporal mesial (memória emocional). Nossos resultados demonstram que o fator epilepsia compromete de forma significativa o desempenho no teste de memória emocional. Por outro lado, a remoção cirúrgica do foco epiléptico promoveu uma melhora na memória emocional desses pacientes, além de diminuir o número de crises

Universal Screening Program in Pregnant Women and Newborns at-Risk for Sickle Cell Disease: First Report from Northern Italy

The implementation of screening programs for early detection of patients with sickle cell disease has become necessary in Italy as a result of the high rate of migration from areas with a high prevalence of the disease (Sub-Saharan Africa, Middle East and the Balkans). Following a pilot study performed in the province of Modena, Italy in 2011\ue2\u80\u932013, an official screening program was established on May 31 2014 for all pregnant women, free-of-charge for the family according to the National Guidelines for Physiological Pregnancy. Hemoglobin (Hb) profiles of pregnant women within 10 weeks of pregnancy, of new mothers at delivery and of the newborns of mothers with variant Hb profiles (newborns at-risk), were evaluated by high performance liquid chromatography (HPLC). Samples from 17,077 new mothers were analyzed and 993 showed alteration of Hb patterns (5.8%) (1.0% Hb AS carriers); of the 1011 at-risk newborns, four (0.4%) carried sickle cell disease and 90 (8.9%) were Hb AS carriers. These data show that early diagnosis of sickle cell disease or carrier status can be obtained in high-risk newborns, providing valuable information on the frequency of these conditions in geographic areas in which the disease is historically rare