Guiding strand passage: DNA-induced movement of the gyrase C-terminal domains defines an early step in the supercoiling cycle

DNA gyrase catalyzes ATP-dependent negative supercoiling of DNA in a strand passage mechanism. A double-stranded segment of DNA, the T-segment, is passed through the gap in a transiently cleaved G-segment by coordinated closing and opening of three protein interfaces in gyrase. T-segment capture is thought to be guided by the C-terminal domains of the GyrA subunit of gyrase that wrap DNA around their perimeter and cause a DNA-crossing with a positive handedness. We show here that the C-terminal domains are in a downward-facing orientation in the absence of DNA, but swing up and rotate away from the gyrase body when DNA binds. The upward movement of the C-terminal domains is an early event in the catalytic cycle of gyrase that is triggered by binding of a G-segment, and first contacts of the DNA with the C-terminal domains, and contributes to T-segment capture and subsequent strand passag

Boston Hospitality Review: Spring 2018

Table of contents: Sustainability In Hospitality? How Legality and Authenticity Impact the Rationale for Integrating Sustainable Practices By Christian E. Hardigree, J.D. -- The Digital Future of the Tourism & Hospitality Industry By Martin Zsarnoczky -- Cutting Through the Online Hospitality Clutter, Part II: Best Practices for Paid Digital Marketing By Leora Lanz and Namrata Sridhar -- Blending Theory and Practice: Experiential Learning in Hospitality Curriculum: A Case Study of Student Projects for Industry Clients By Michael Oshins and Joel Brown

The positive effect of blue luminescent pathways on urban park visitor’s affective states: A virtual reality online study measuring facial expressions and self-reports

The colour blue often elicits feelings of calmness and contentment, for which evidence has largely been provided in daytime settings. It is unclear whether pathways illuminated in blue, for example, in urban recreational park areas at night confers the same positive impact on night time park visitors. To tackle this open empirical question, we investigated how adding blue self-luminous pavement to park lighting at night time affects park visitors’ emotions compared to conventional white street light illumination. Our goal is to inform design decisions aimed at enhancing the emotional well-being of people outdoors at night in urban environments. Participants’ emotional response was captured at four different time points while watching a video of a walk in a virtual urban park at night, which was lit with white street lights only or with the addition of blue luminescent pavement on the walked paths (between-subject design). To capture visitor’s emotions, we used a simplified version of the Geneva Emotion Wheel (GEW) instrument and online facial expression recognition technology as subjective (self-reports) and objective (physiological) measures of emotion, respectively. The results of the GEW self-reports showed that the addition of a blue self-luminous pavement in a park during night time yielded more positive affect than standard white lighting in park visitors for the first half of the walk. In the second half of the walk through the park, participants’ affective states seemed to equalize between the two lighting conditions. In contrast, sensory data on facial expressions indicated no difference between participants’ emotional states over the whole walk in the two experimental conditions. Consistent with the positive emotional state perceived in the second half of the walk, the state of relaxation experienced after the walk also did not differ between the two lighting conditions. Furthermore, participants’ relaxation judgements after the park walk were more negative overall for females than the more neutral ratings of males. Our results highlight the importance of lighting colour at night for the design of future affect-smart cities that may consider individual and group characteristics with the ultimate intent of promoting public well-being

The role of European National Forest Inventories for international forestry reporting

International audienceAbstractKey messageDespite agreements on definitions, the national data provided for international reporting are lacking comparability. To address this limitation the European National Forest Inventory Network has established criteria to harmonise definitions and to provide tools to transform national data into internationally comparable data.ContextForest reporting presents a series of challenges for countries, owing to diverse processes at international level such as the Global Forest Resources Assessment (FRA), Convention on Biological Diversity, United Nations Framework Convention on Climate Change and its Kyoto Protocol. Further challenges are faced at European level with Forest Europe and policy needs.AimsThe aim of this paper is to provide a comprehensive review of the national and international forest reporting processes and of the role of the National Forest Inventories (NFIs) and the long-associated challenges resulting from a lack of comparability in definitions used. In addition, there is a discussion on the role of the European National Forest Inventory Network (ENFIN) as a facilitator for enhancing harmonization and comparability of national data and the ancillary information required to monitor European forestry-related policies.MethodsNFIs take part to international reporting processes as providers of information. They are correspondent to the FRA process, and then they know very well the context of harmonization. Participating in the ENFIN research projects, NFIs, and particularly authors, conducted a screening exercise on harmonization status at European and World level.ResultsThis review article is a synthesis of the main findings of the abovementioned screening exercise. It highlights the main gaps in terms of comparability of result in international reporting. Thanks to ENFIN harmonization research project, it gives same ways of working as a possible benchmark for the rest of the world.ConclusionBased on the international reporting exercises, their interactions, and impacts on new forestry policy requirements, the need for a strengthened harmonization process can clearly be demonstrated. Due to European policy needs, research work within ENFIN has been initiated to develop tools for building comparable results at international level. This work is an important benchmark particularly for countries outside Europe from which to base future harmonization work

The rise and fall of the Phytophthora infestans lineage that triggered the Irish potato famine

Phytophthora infestans, the cause of potato late blight, is infamous for having triggered the Irish Great Famine in the 1840s. Until the late 1970s, P. infestans diversity outside of its Mexican center of origin was low, and one scenario held that a single strain, US-1, had dominated the global population for 150 years; this was later challenged based on DNA analysis of historical herbarium specimens. We have compared the genomes of 11 herbarium and 15 modern strains. We conclude that the nineteenth century epidemic was caused by a unique genotype, HERB-1, that persisted for over 50 years. HERB-1 is distinct from all examined modern strains, but it is a close relative of US-1, which replaced it outside of Mexico in the twentieth century. We propose that HERB-1 and US-1 emerged from a metapopulation that was established in the early 1800s outside of the species' center of diversity.Comment: To be published in eLIF

Crystallographic snapshots of sulfur insertion by lipoyl synthase

Lipoyl synthase (LipA) catalyzes the insertion of two sulfur atoms at the unactivated C6 and C8 positions of a protein-bound octanoyl chain to produce the lipoyl cofactor. To activate its substrate for sulfur insertion, LipA uses a [4Fe-4S] cluster and S-adenosylmethionine (AdoMet) radical chemistry; the remainder of the reaction mechanism, especially the source of the sulfur, has been less clear. One controversial proposal involves the removal of sulfur from a second (auxiliary) [4Fe-4S] cluster on the enzyme, resulting in destruction of the cluster during each round of catalysis. Here, we present two high-resolution crystal structures of LipA from Mycobacterium tuberculosis: one in its resting state and one at an intermediate state during turnover. In the resting state, an auxiliary [4Fe-4S] cluster has an unusual serine ligation to one of the irons. After reaction with an octanoyllysine-containing 8-mer peptide substrate and 1 eq AdoMet, conditions that allow for the first sulfur insertion but not the second insertion, the serine ligand dissociates from the cluster, the iron ion is lost, and a sulfur atom that is still part of the cluster becomes covalently attached to C6 of the octanoyl substrate. This intermediate structure provides a clear picture of iron-sulfur cluster destruction in action, supporting the role of the auxiliary cluster as the sulfur source in the LipA reaction and describing a radical strategy for sulfur incorporation into completely unactivated substrates. Keywords: iron–sulfur cluster; radical SAM enzyme; lipoic acidNational Science Foundation (U.S.) (Grant MCB-0543833

Polarization of the WMAP Point Sources

The detection of polarized sources in the WMAP 5-year data is a very difficult task. The maps are dominated by instrumental noise and only a handful of sources show up as clear peaks in the Q and U maps. Optimal linear filters applied at the position of known bright sources detect with a high level of significance a polarized flux P from many more sources, but estimates of P are liable to biases. Using a new technique, named the "filtered fusion technique", we have detected in polarization, with a significance level greater than 99.99% in at least one WMAP channel, 22 objects, 5 of which, however, do not have a plausible low radio frequency counterpart and are therefore doubtful. Estimated polarized fluxes P < 400 mJy at 23 GHz were found to be severely affected by the Eddington bias. The corresponding polarized flux limit for Planck/LFI at 30 GHz, obtained via realistic simulations, is 300 mJy. We have also obtained statistical estimates of, or upper limits to the mean polarization degrees of bright WMAP sources at 23, 33, 41, and 61 GHz, finding that they are of a few percent.Comment: 10 pages, 6 figures. Accepted for publication in Ap

From ADEPT to AristaFlow BPM Suite: A Research Vision has become Reality

During the last decade we have developed the ADEPT next generation process management technology. Its features and its different prototype versions attracted a number of companies. However, an enterprise cannot base the implementation of its process-aware information system (PAIS) on an experimental prototype, especially if maintenance and further development are not assured. At the beginning of 2008, therefore, we founded a spin-off as joint venture with industrial partners to transfer ADEPT into an industrial-strength product version called AristaFlow BPM Suite, and to provide maintenance support for it. The product version is now available for academic and industrial use