Streptococcus suis in German pig holdings—conventional and molecular detection

Streptococcus suis (S. suis) is a zoonotic agent worldwide. Pigs are the main reservoir, mostly asymptomatic. Humans get the infection by contact and consumption of contaminated meat and meat products. In this study, samples from 38 pig carcasses fit for human consumption from 17 holdings were arbitrarily selected. From each carcass, seven tissue samples were taken and examined for the presence of S. suis, using conventional microbiology and PCR. In addition, virulence-associated factors (epf, arcA, sly, mrp) were tested with PCR. More isolates were PCR-positive for S. suis as compared to conventional testing, mostly in samples from the heart and from the mandibular lymphnodes. All isolates were epf negative, combinations of arcA, sly and mrp were found in some isolates. Six isolates were positive for arcA and mrp, five for arcA and sly. For three isolates the triple combination arcA + mrp + sly was found. These isolates originated from different pigs

Tracing back to Sources of MAIC Using Farm records and Lab Techniques

For the implementation of tracing back systems (particular agents, unwanted observation and other), detection techniques should be available. For MAIC, a step-by-step approach was established, combining visual observation, lab based PCR-identification, tracing back to the farm of origin and finally the search for potential ports of entry

Differences in code terminology and frequency of findings in meat inspection of finishing pigs in seven European countries

The overall objectives of meat inspection are to contribute to food safety, animal welfare, and animal health. In the European Union (EU), there is a request for a modernised meat inspection system that addresses these objectives in a more valid, feasible and cost-effective way than does the traditional system. One part of the modernisation deals with the coding system to register meat inspection findings. Although unified standards are set at the EU level for judgement criteria regarding fitness of meat for consumption, different national systems are in force. The question is the extent of the differences and whether there is a basis for harmonisation. To investigate this, information was gathered about the code systems in Denmark, Finland, Germany, Italy, Norway, Portugal and Spain. Moreover, meat inspection data covering pigs slaughtered in 2019 were collected. A comparison of the number of codes available, the terminology and the frequencies of the findings registered was undertaken. Codes with a similar meaning were grouped. Hereby, two lists were compiled showing the most common codes leading to total and to partial condemnation. Substantial variations in the percentage of condemned pigs and in the terms used were identified, and possible reasons behind this are discussed. Moreover, a strengths-weaknesses-opportunities-threats (SWOT)-like analysis was applied to the coding systems. Finally, the reasons for unfitness of meat given in the EU Food Inspection Regulation 2019/627 were compared to the national code lists. The results show the systems in force varied substantially, and each system had its advantages and disadvantages. The diverse terminology observed made it a challenge to compare data between countries. Development of harmonised terminology for meat inspection findings is suggested, enabling comparison of data between abattoirs, regions, and countries, while respecting the national epidemiological situation, the local food safety culture, and the trade agreements in force.Peer reviewe

Differences in code terminology and frequency of findings in meat inspection of finishing pigs in seven European countries

The overall objectives of meat inspection are to contribute to food safety, animal welfare, and animal health. In the European Union (EU), there is a request for a modernised meat inspection system that addresses these objectives in a more valid, feasible and cost-effective way than does the traditional system. One part of the modernisation deals with the coding system to register meat inspection findings. Although unified standards are set at the EU level for judgement criteria regarding fitness of meat for consumption, different national systems are in force. The question is the extent of the differences and whether there is a basis for harmonisation. To investigate this, information was gathered about the code systems in Denmark, Finland, Germany, Italy, Norway, Portugal and Spain. Moreover, meat inspection data covering pigs slaughtered in 2019 were collected. A comparison of the number of codes available, the terminology and the frequencies of the findings registered was undertaken. Codes with a similar meaning were grouped. Hereby, two lists were compiled showing the most common codes leading to total and to partial condemnation. Substantial variations in the percentage of condemned pigs and in the terms used were identified, and possible reasons behind this are discussed. Moreover, a strengths-weaknesses-opportunities-threats (SWOT)-like analysis was applied to the coding systems. Finally, the reasons for unfitness of meat given in the EU Food Inspection Regulation 2019/627 were compared to the national code lists. The results show the systems in force varied substantially, and each system had its advantages and disadvantages. The diverse terminology observed made it a challenge to compare data between countries. Development of harmonised terminology for meat inspection findings is suggested, enabling comparison of data between abattoirs, regions, and countries, while respecting the national epidemiological situation, the local food safety culture, and the trade agreements in force

Neutrophil Paralysis in Plasmodium vivax Malaria

Plasmodium vivax is responsible for approximately 60–80% of the malaria cases in the world, and contributes to significant social and economic instability in the developing countries of Latin America and Asia. The pathogenesis of P. vivax malaria is a consequence of host derived inflammatory mediators. Hence, a better understanding of the mechanisms involved in induction of systemic inflammation during P. vivax malaria is critical for the clinical management and prevention of severe disease. The innate immune receptors recognize Plasmodium sp. and initiate a broad spectrum of host defense mechanisms that mediate resistance to infection. However, the innate immune response is the classic “two-edged sword”, and clinical malaria is associated with high levels of circulating pro-inflammatory cytokines. Our findings show that both monocytes and neutrophils are highly activated during malaria. Monocytes produced high levels of IL-1β, IL-6 and TNF-α during acute malaria. On the other hand, neutrophils were a poor source of cytokines, but displayed an enhanced phagocytic activity and superoxide production. Unexpectedly, we noticed an impaired chemotaxis of neutrophils towards an IL-8 (CXCL8) gradient. We proposed that neutrophil paralysis is in part responsible for the enhanced susceptibility to bacterial infection observed in malaria patients

Streptococcus suis in German pig holdings—conventional and molecular detection

Streptococcus suis (S. suis) is a zoonotic agent worldwide. Pigs are the main reservoir, mostly asymptomatic. Humans get the infection by contact and consumption of contaminated meat and meat products. In this study, samples from 38 pig carcasses fit for human consumption from 17 holdings were arbitrarily selected. From each carcass, seven tissue samples were taken and examined for the presence of S. suis, using conventional microbiology and PCR. In addition, virulence-associated factors (epf, arcA, sly, mrp) were tested with PCR. More isolates were PCR-positive for S. suis as compared to conventional testing, mostly in samples from the heart and from the mandibular lymphnodes. All isolates were epf negative, combinations of arcA, sly and mrp were found in some isolates. Six isolates were positive for arcA and mrp, five for arcA and sly. For three isolates the triple combination arcA + mrp + sly was found. These isolates originated from different pigs.</p

Tracing back to Sources of MAIC Using Farm records and Lab Techniques

For the implementation of tracing back systems (particular agents, unwanted observation and other), detection techniques should be available. For MAIC, a step-by-step approach was established, combining visual observation, lab based PCR-identification, tracing back to the farm of origin and finally the search for potential ports of entry.</p

Persistent human KIT receptor signaling disposes murine placenta to premature differentiation resulting in severely disrupted placental structure and functionality

10.3390/ijms21155503International Journal of Molecular Sciences2115Jan-1