Effect of a simple intervention on hand hygiene related diseases in preschools in South Africa: research protocol for an intervention study

Abstract : Introduction Hand hygiene (HH) related illnesses such as diarrhoea and respiratory diseases, contribute to the burden of disease and are included in the top five causes of mortality in children under 5 years in South Africa. Children attending preschools are more susceptible to these infections due to the higher number of children in preschools. HH interventions have shown to reduce HH-related diseases by improving HH practices. In South Africa, there are no documented HH interventions or studies in children under 5 years. The purpose of the study is to determine whether an HH intervention can reduce HH-related diseases among 4–5-year- old preschool children and to improve HH practices in these children, their caregivers and their parents..

The effect of a simple intervention on hand hygiene related diseases in preschools

Abstract: Background Worldwide, hand hygiene related diseases such as diarrhoea and upper respiratory diseases are still reported as a leading cause of death in children under 5 years. Washing hands with soap and water can be considered key to reducing the burden of hand hygiene (HH) related diseases and in so doing can contribute towards improved health in children under five years of age. At the time of the study, hand washing with soap at critical times was still only practiced by 19.0% of people worldwide. The need for water, sanitation and hygiene (WASH) interventions to reduce HH-related diseases has shown to be key in all sectors of the community, be it at a household level, at preschools and schools or in health care settings. These WASH interventions can include supply of infrastructure, provision of the necessary equipment, improved water and sanitation or simple education programmes to encourage communities to wash their hands at critical times. Preschoolers can benefit from HH interventions by reducing the burden of disease and absenteeism. Many school hours are lost due to illness and absenteeism which are counterproductive towards reaching the developmental milestones essential at a young age, to produce a child who can participate actively and productively in later school years. This study made use of a simple, cost-effective and entertaining intervention aimed at parents, preschoolers and their caregivers or teachers in order to determine a reduction of HH-related diseases in preschool children. Methods This study was a controlled intervention study conducted at randomly selected preschools in Ekurhuleni, Gauteng. The schools were randomly placed into an intervention group (IG) and a control group (CG). Data was collected pre- and post-intervention from parents, caregivers and preschool children. Data collection tools included questionnaires for parents and caregivers, observations of HH opportunities in the classroom, hygiene inspections of the preschools and the recording of children’s illnesses by caregivers in a symptom register. Hand ““bag-wash”” samples were collected pre-intervention; post-intervention pre- hand wash and post-intervention post-hand wash. The analysis of the “bag-wash” samples was run in an approved laboratory using strict microbiological specifications. A pilot study was conducted in preschools not included in the main study in order to test the effectiveness of the data collection tools and intervention process. The necessary adjustments were made to the data collection tools and intervention prior to the start of the main study. The intervention was administered to IG preschools and included interactive, entertaining learning activities for the children and electronic messages sent to parents and caregivers. The intervention was conducted using a cost-effective “Magic Box” and GloGerm®. Children were taught to wash hands according to the World Health Organization (WHO) approved method and were then able to see if hands had been wash effectively...D.Tech. (Environmental Health

Numerical optimization of integrating cavities for diffraction-limited millimeter-wave bolometer arrays

Far-infrared to millimeter-wave bolometers designed to make astronomical observations are typically encased in integrating cavities at the termination of feedhorns or Winston cones. This photometer combination maximizes absorption of radiation, enables the absorber area to be minimized, and controls the directivity of absorption, thereby reducing susceptibility to stray light. In the next decade, arrays of hundreds of silicon nitride micromesh bolometers with planar architectures will be used in ground-based, suborbital, and orbital platforms for astronomy. The optimization of integrating cavity designs is required for achieving the highest possible sensitivity for these arrays. We report numerical simulations of the electromagnetic fields in integrating cavities with an infinite plane-parallel geometry formed by a solid reflecting backshort and the back surface of a feedhorn array block. Performance of this architecture for the bolometer array camera (Bolocam) for cosmology at a frequency of 214 GHz is investigated. We explore the sensitivity of absorption efficiency to absorber impedance and backshort location and the magnitude of leakage from cavities. The simulations are compared with experimental data from a room-temperature scale model and with the performance of Bolocam at a temperature of 300 mK. The main results of the simulations for Bolocam-type cavities are that (1) monochromatic absorptions as high as 95% are achievable with <1% cross talk between neighboring cavities, (2) the optimum absorber impedances are 400 Ω/sq, but with a broad maximum from ~150 to ~700 Ω/sq, and (3) maximum absorption is achieved with absorber diameters ≥1.5λ. Good general agreement between the simulations and the experiments was found

Survey of southern Missouri Plethodontidae in Ozark caves

Plethodontidae is a large family of salamanders with a distribution ranging from eastern North Americato northern South America. Three genera and nine species occur in Missouri, most of which are typically found in cavesfor at least part of their lives. These species have been recorded to emerge from hibernation in early April and throughoutMay. This study shares the species abundance and diversity in ten Missouri Ozark caves: seven near St. Louis andthree near Springfield. Each cave was surveyed at least once between November 2021 and September 2022 using visualencounter surveys. Observational data were recorded without handling any individuals to minimize disturbance. Thethree caves near St. Louis had few to no salamanders present in early April, despite optimal weather conditions, buttended to have larger populations throughout the summer. Two caves near Springfield held at least a dozen individualsof multiple species in mid-April. Few individuals were recorded in the most southwestern cave sampled, potentiallydue to pollution or recent flooding. Our findings could aid others studying plethodontid salamanders to determine theprime conditions for emergence from hibernation, optimal habitats for different species in each cave zone, and potentialbiological or chemical impacts that could affect salamander abundance in caves

Biomaterials Influence Macrophage-Mesenchymal Stem Cell Interaction In Vitro

Background: Macrophages and mesenchymal stem cells (MSCs) are important cells in wound healing. We hypothesized that the cross-talk between macrophages and adipose tissue-derived MSCs (ASCs) is biomaterial dependent, thereby influencing processes involved in wound healing. Materials and Methods: The effect of macrophages cultured on polypropylene (PP) or polyethylene terephthalate coated with a collagen film (PET/Col) on ASCs in monolayer or on the same material was examined either through conditioned medium (CM) or in a direct coculture. ASC proliferation, collagen production, and gene expression were examined. As comparison, the effect of macrophages stimulated with lipopolysaccharide (LPS) and interferon gamma (IFN gamma) [M(LPS/IFN gamma)] or interleukin (IL) 4 [M(IL-4)] on ASCs was examined. Results: Macrophage-CM increased collagen deposition, proliferation, and gene expression of MMP1, PLOD2, and PTGS2 in ASCs, irrespective of the material. Culturing ASCs and macrophages in coculture when only macrophages were on the materials induced the same effects on gene expression. When both ASCs and macrophages were cultured on biomaterials, PP induced COL1A1 and MMP1 more than PET/Col. M(LPS/IFN gamma) CM increased PLOD2, MMP1, and PTGS2 and decreased TGFB in ASCs more than the M(IL-4) CM. Conclusion: Biomaterials influence wound healing by influencing the interaction between macrophages and ASCs. We provided more insight into the behavior of different cell types during wound healing. This behavior appears to be biomaterial specific depending on which cell type interacts with the biomaterial. As such, the biomaterial will influence tissue regeneration

Biomaterials Influence Macrophage-Mesenchymal Stem Cell Interaction In Vitro

Background: Macrophages and mesenchymal stem cells (MSCs) are important cells in wound healing. We hypothesized that the cross-talk between macrophages and adipose tissue-derived MSCs (ASCs) is biomaterial dependent, thereby influencing processes involved in wound healing. Materials and Methods: The effect of macrophages cultured on polypropylene (PP) or polyethylene terephthalate coated with a collagen film (PET/Col) on ASCs in monolayer or on the same material was examined either through conditioned medium (CM) or in a direct coculture. ASC proliferation, collagen production, and gene expression were examined. As comparison, the effect of macrophages stimulated with lipopolysaccharide (LPS) and interferon gamma (IFN gamma) [M(LPS/IFN gamma)] or interleukin (IL) 4 [M(IL-4)] on ASCs was examined. Results: Macrophage-CM increased collagen deposition, proliferation, and gene expression of MMP1, PLOD2, and PTGS2 in ASCs, irrespective of the material. Culturing ASCs and macrophages in coculture when only macrophages were on the materials induced the same effects on gene expression. When both ASCs and macrophages were cultured on biomaterials, PP induced COL1A1 and MMP1 more than PET/Col. M(LPS/IFN gamma) CM increased PLOD2, MMP1, and PTGS2 and decreased TGFB in ASCs more than the M(IL-4) CM. Conclusion: Biomaterials influence wound healing by influencing the interaction between macrophages and ASCs. We provided more insight into the behavior of different cell types during wound healing. This behavior appears to be biomaterial specific depending on which cell type interacts with the biomaterial. As such, the biomaterial will influence tissue regeneration

Biomaterials Influence Macrophage-Mesenchymal Stem Cell Interaction In Vitro

Background: Macrophages and mesenchymal stem cells (MSCs) are important cells in wound healing. We hypothesized that the cross-talk between macrophages and adipose tissue-derived MSCs (ASCs) is biomaterial dependent, thereby influencing processes involved in wound healing. Materials and Methods: The effect of macrophages cultured on polypropylene (PP) or polyethylene terephthalate coated with a collagen film (PET/Col) on ASCs in monolayer or on the same material was examined either through conditioned medium (CM) or in a direct coculture. ASC proliferation, collagen production, and gene expression were examined. As comparison, the effect of macrophages stimulated with lipopolysaccharide (LPS) and interferon gamma (IFNγ) [M(LPS/IFNγ)] or interleukin (IL) 4 [M(IL-4)] on ASCs was examined. Results: Macrophage-CM increased collagen deposition, proliferation, and gene expression of MMP1, PLOD2, and PTGS2 in ASCs, irrespective of the material. Culturing ASCs and macrophages in coculture when only macrophages were on the materials induced the same effects on gene expression. When both ASCs and macrophages were cultured on biomaterials, PP induced COL1A1 and MMP1 more than PET/Col. M(LPS/IFNγ) CM increased PLOD2, MMP1, and PTGS2 and decreased TGFB in ASCs more than the M(IL-4) CM. Conclusion: Biomaterials influence wound healing by influencing the interaction between macrophages and ASCs. We provided more insight into the behavior of different cell types during wound healing. This behavior appears to be biomaterial specific depending on which cell type interacts with the biomaterial. As such, the biomaterial will influence tissue regeneration

Bolocam: a millimeter-wave bolometric camera

We describe the design of Bolocam, a bolometric camera for millimeter-wave observations at the Caltech Submillimeter Observatory. Bolocam will have 144 diffraction-limited detectors operating at 300 mK, an 8 arcminute field of view, and a sky noise limited NEFD of approximately 35 mJy Hz^(-1/2) per pixel at λ = 1.4 mm. Observations will be possible at one of (lambda) equals 1.1., 1.4, or 2.1 mm per observing run. The detector array consists of sensitive NTD Ge thermistors bonded to silicon nitride micromesh absorbers patterned on a single wafer of silicon. This is a new technology in millimeter-wave detector array construction. To increase detector packing density, the feed horns will be spaced by 1.26 fλ (at λ = 1.4 mm), rather than the conventional 2fλ . DC stable read out electronics will enable on-the-fly mapping and drift scanning. We will use Bolocam to map Galactic dust emission, to search for protogalaxies, and to observe the Sunyaev- Zel'dovich effect toward galaxy clusters

Applying a WASH risk assessment tool in a rural South African setting to identify risks and opportunities for climate resilient communities

Climate change threatens the health and well-being of populations. We conducted a risk assessment of two climate-related variables (i.e., temperature and rainfall) and associated water, sanitation and hygiene (WASH)-related exposures and vulnerabilities for people living in Mopani District, Limpopo province, South Africa. Primary and secondary data were applied in a qualitative and quantitative assessment to generate classifications of risk (i.e., low, medium, or high) for components of hazard/threat, human exposure, and human vulnerability. Climate-related threats were likely to impact human health due to the relatively high risk of waterborne diseases and WASH-associated pathogens. Vulnerabilities that increased the susceptibility of the population to these adverse outcomes included environmental, human, physical infrastructure, and political and institutional elements. People of low socio-economic status were found to be least likely to cope with changes in these hazards. By identifying and assessing the risk to sanitation services and water supply, evidence exists to inform actions of government and WASH sector partners. This evidence should also be used to guide disaster risk reduction, and climate change and human health adaptation planning.DATA AVAILABILITY STATEMENT : The laboratory sample data are available from the National Health Laboratory Services. All hazard-related data were extracted from publicly available manuscripts as cited in the text.The South African Medical Research Council and the National Research Foundation. The A.P.C. was funded by the South African Medical Research Council.https://www.mdpi.com/journal/ijerphGeography, Geoinformatics and Meteorolog

Current status of Bolocam: a large-format millimeter-wave bolometer camera

We describe the design and performance of Bolocam, a 144-element, bolometric, millimeter-wave camera. Bolocam is currently in its commissioning stage at the Caltech Submillimeter Observatory. We compare the instrument performance measured at the telescope with a detailed sensitivity model, discuss the factors limiting the current sensitivity, and describe our plans for future improvements intended to increase the mapping speed